A fungicidal composition containing thiamethoxam and ipflufenoquin

Abstract

The present application provides a fungicidal active ingredient composition containing thiamethoxam and Ipflufenoquin, wherein the weight ratio of thiamethoxam to Ipflufenoquin in the fungicidal active ingredient composition is 36:1 to 1:36. The fungicidal composition of the present application has synergistic effect on the prevention and treatment of agricultural diseases within the defined ratio range. The present application also relates to a fungicide comprising the fungicidal active ingredient composition, and a method and application of the fungicidal active ingredient composition or the fungicide for preventing and treating agricultural diseases.

Description

Technical Field

[0001] This invention belongs to the agricultural field, specifically relating to a bactericidal composition containing thiamethoxam and Ipflufenoquin and its application. Background Technology

[0002] Thiamethoxam (also known as 1,2-benzisothiazolin-3-one), belonging to the isothiazole heterocyclic compound class, is a novel, highly effective, low-toxicity, broad-spectrum fungicide. Its mechanism of action primarily involves binding to anions or sulfhydryl groups on the surface of pathogen cell membranes, disrupting the pathogen's nuclear structure, damaging protein and cell membrane synthesis systems, interfering with pathogen cell metabolism, inhibiting pathogen reproduction, and ultimately leading to pathogen death. This compound exhibits good control effects against bacterial and fungal diseases affecting crops.

[0003] Ipflufenoquin is a novel quinoline-based broad-spectrum fungicide developed by Nippon Soda Co., Ltd. It has good control effects on dozens of important crop diseases caused by pathogenic fungi such as oomycetes, ascomycetes, deuteromycetes, and basidiomycetes. Its control range covers cucumber downy mildew and powdery mildew, wheat scab, rice blast, apple scab, and grape gray mold, among others.

[0004] CN112189663A discloses a fungicidal and bactericidal composition containing malononitrile oxime ethers and thiamethoxam, which is used to control various diseases such as rice bacterial blight, tomato bacterial wilt, cucumber powdery mildew, and rice bacterial leaf streak, and has a synergistic effect.

[0005] CN114365744A discloses a fungicide composition comprising thiamethoxam and difenoconazole, and specifically discloses the use of the fungicide composition for controlling fungi and bacteria on cereals, legumes, fruit trees and fruits, vegetables, root vegetables, forage grasses, turfgrass or lawns, spice crops, and flowering plants.

[0006] Xia Xiyuan et al. (Synthesis and fungicidal activity of novel fungicide ipflufenoquin, Pesticides, Vol. 62, No. 8) disclosed that the novel fungicide ipflufenoquin has good control effects on a variety of plant diseases such as cucumber downy mildew, cucumber anthracnose, wheat scab, and cucumber powdery mildew.

[0007] Downy mildew of cucumber is a devastating airborne disease caused by a pathogen of the oomycete class. The pathogen is *Pseudomonas columbarium*, an obligate parasite. Infection primarily occurs during the mature stage of cucumber plants, especially after flowering and fruiting. The most prominent symptoms appear on the leaves, initially as small, pale yellow spots. As these spots enlarge, they become polygonal yellow patches due to vein restriction. In humid conditions, a purplish-gray to black mold layer grows on the corresponding areas on the underside of the leaves. This disease causes significant damage to cucumbers. In later stages, the lesions merge, causing the entire leaf to wither and curl, severely weakening the plant's photosynthesis, leading to slow and deformed fruit growth, and even the death of the entire plant.

[0008] Fusarium head blight of wheat is a devastating ear disease that not only reduces yield but also poses a significant threat to human and animal health due to the toxins produced by the pathogen. Initially, water-soaked brown spots appear on the glumes, which later expand, causing partial or complete yellowing of the spikelets. In humid conditions, a pink or orange-yellow mold layer develops on the affected areas, and small black granules may appear in later stages. Infected grains become shriveled and lose their luster. The pathogen overwinters on crop residues such as corn stalks and rice stubble, and the disease's spread is closely related to weather conditions during the flowering period.

[0009] Currently, there are no reports of existing technologies involving the combination of thiamethoxam and Ipflufenoquin. Summary of the Invention

[0010] The purpose of this invention is to screen effective compound agents for common but difficult-to-control diseases in agricultural production, such as cucumber downy mildew and wheat scab, using existing technologies. Through research, the inventors discovered that the combination of thiamethoxam and Ipflufenoquin has a significant synergistic effect, effectively controlling various agricultural diseases, including cucumber downy mildew and wheat scab, and based on this, this invention was completed.

[0011] One aspect of this invention relates to a bactericidal active ingredient composition, wherein the bactericidal active ingredient composition contains two active ingredients: thiamethoxam and Ipflufenoquin.

[0012] In the bactericidal active ingredient composition of the present invention, the weight ratio of thiamethoxam to Ipflufenoquin is 36:1 to 1:36, preferably 18:1 to 1:18. For example: 18:1, 17:1, 16:1, 15:1, 14:1, 13:1, 12:1, 11:1, 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1:11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18.

[0013] In a preferred embodiment of the present invention, the weight ratio of thiamethoxam to Ipflufenoquin is further preferably 9:1 to 1:9, and even more preferably 4.5:1 to 1:4.5.

[0014] In addition to the two active ingredients, thiamethoxam and Ipflufenoquin, the bactericidal active ingredient composition of the present invention may also contain other known bactericides, such as: Triazoles: Tebuconazole, Hexaconazole, Propiconazole, Prothioconazole, Triadimefon, Bisbenzyltriazole, Cycloconazole, Tebuconazole, Zimbabenazole, Oxytetracycline, Cyproconazole, Flusilazole, Tebuconazole, Cycloconazole, Paclobutrazol, Tebuconazole, Silozoline, Flufenoxuron; Methoxyacrylates: azoxystrobin, pyraclostrobin, oxadiazon, pyraclostrobin, difenoconazole, cyproconazole, pyraclostrobin; Carboxamides; Benzoyl sulfadiazine, Benzoyl sulfadiazine, Mefenoxam, Cyclopyralid, Cyclopyralid, Fluopyram, Furazolidone, Isothiazamide, Propyrazosulfuron, Metalaxyl, Metalaxyl-M, Mefenoxam, Bromofluopyram, Fluopyram, Thiamethoxam; Carboxymorpholines: dimethomorpholine, flumethomorpholine; Benzimidazoles: Carbendazim, Benomyl, Thiamethoxam, Mefenoxam, Thiamethoxam; Dicarboxyimide derivatives: iprodione, procymidone, vinclozolin; Amides: thiamethoxam, flumorpholine, dimethomorpholine, silthiamethoxam, chlorpyrifos; Pyrroles: Seed dressing pyrrole, fludioxonil; Pyrimidine derivatives: pyrimethanil, fluazolidinium, sulfadiazine, cyprodinil, fluazolidinium, isopyram, pyrimethanil, pyrimethanil, chlorpyrifos, chlorpyrifos, dimethoprim; Carbamates: Mancozeb, Mancozeb, Mancozeb, Mancozeb, Mancozeb, Zinc thiram, Mancozeb, Zinc thiram; Organochlorine compounds: chlorothalonil, bacteriostatic agent, dichlorophenol, sulfadiazine, hexachlorobenzene, pendimethalin, pentachlorophenol and its salts, tetrachlorophthalide, pentachloronitrobenzene, methyl thiophanate.

[0015] In a preferred embodiment of the present invention, the bactericidal active ingredient composition consists of only two active ingredients: thiamethoxam and Ipflufenoquin.

[0016] In this invention, the combination of thiamethoxam and Ipflufenoquin, two active ingredients, exhibits a significant synergistic effect in the control of agricultural diseases. This synergistic effect is particularly pronounced in the control of cucumber downy mildew and wheat scab, making it the preferred formulation of this invention.

[0017] Another aspect of the present invention relates to a fungicide, wherein the fungicide comprises the fungicide active ingredient composition described in the present invention, and agriculturally acceptable adjuvants and / or carriers.

[0018] In one embodiment of the present invention, the total weight percentage of bactericidal active ingredients in the bactericide is 1-80%, preferably 5-60%, and more preferably 5-40%.

[0019] In one embodiment of the present invention, the bactericide can be prepared into agriculturally usable formulations such as emulsifiable concentrates, wettable powders, water-dispersible granules, suspension concentrates, microemulsions, and water-in-oil emulsions, preferably prepared as a suspension concentrate.

[0020] In this invention, the processing and preparation methods of the pesticide formulations are all well known to those skilled in the art, and the processing equipment used is also commonly used in pesticide formulation processing.

[0021] In one embodiment of the present invention, the agriculturally acceptable adjuvant and / or carrier includes one or more of the following: solvent, cosolvent, dispersant, wetting agent, emulsifier, thickener, binder, antifreeze, defoamer, stabilizer, disintegrant, and filler. Wherein: The solvent includes one or more of the following: dimethylformamide, dimethyl sulfoxide, acetone, xylene, dichloromethane, ethyl acetate, cyclohexane, cyclohexanone, N-methylpyrrolidone, coconut oil fatty acid methyl ester, cyclohexanol, methyl glycol ether, and acetonitrile.

[0022] The co-solvent includes one or more of methanol, ethanol, isopropanol, and n-butanol.

[0023] The dispersant comprises one or more of the following: lignin sulfonate, nonylphenol polyoxyethylene ether, polyoxyethylene alkyl aryl ether, polyoxyethylene lanolin alcohol, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, alkyl naphthalene sulfonate, sodium salt of naphthalene sulfonic acid formaldehyde condensate, sodium p-hydroxyphenyl lignin sulfonate, fatty alcohol polyoxyethylene ether sulfate, alkylphenol polyoxyethylene ether formaldehyde condensate, alkyl dimethyl benzyl ammonium salt, p-methoxy fatty amide benzene sulfonic acid, p-methoxy fatty amide benzene sulfonate, and polyoxyethylene sorbitan fatty acid ester.

[0024] The wetting agent includes one or more of the following: sodium dodecyl sulfate, calcium dodecylbenzene sulfonate, sodium lauroyl sarcosinate, fatty alcohol polyoxyethylene ether, dioctyl sulfosuccinate, polyoxyethylene alkyl aryl ether phosphate, trisiloxane polyoxyethylene ether, sodium isooctyl succinate sulfonate, sodium oleoyl methyl taurate, triphenylethylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, polyoxyethylene fatty acid ester, and sorbitan fatty acid ester.

[0025] The emulsifier includes one or more of the following: lignin sulfonate, sodium dodecyl sulfate, sodium dodecylbenzene sulfonate, calcium dodecylbenzene sulfonate, naphthalene sulfonate formaldehyde condensate, styrene-phenyl polyoxyethylene ether, fatty alcohol polyoxyethylene ether, polyoxyethylene alkyl ether, polyoxyethylene sorbitan fatty acid ester, nonylphenol polyoxyethylene ether phosphate, styrene polyoxyethylene ether ammonium sulfate, sorbitan monostearate, and dehydrated sorbitan monostearate polyoxyethylene ether.

[0026] The thickener includes one or more of the following: carboxymethyl cellulose, sodium carboxymethyl cellulose, gum arabic, xanthan gum, gelatin, magnesium aluminum silicate, and sodium alginate.

[0027] The adhesive includes one or more of gum arabic, xanthan gum, starch, carboxymethyl cellulose, carboxyethyl cellulose, and gelatin.

[0028] The antifreeze includes one or more of ethylene glycol, propylene glycol, glycerol, and polyethylene glycol.

[0029] The defoamer includes one or more of the following: butyl phosphate, isobutyl phosphate, n-octanol, and silicone.

[0030] The stabilizer includes one or more of the following: triphenyl phosphite, epoxidized soybean oil, epoxidized linseed oil, dialkyl succinate sulfonate, butyl glycidyl ether, etc.

[0031] The disintegrant includes one or more of the following: sodium bicarbonate, ammonium sulfate, sodium carboxymethyl starch, sodium sulfate, calcium sulfate, and urea.

[0032] The filler / carrier includes solid filler / carrier and liquid filler / carrier, specifically including one or more of the following: kaolin, bentonite, diatomaceous earth, attapulgite, montmorillonite, calcium carbonate, clay, water, soybean oil, corn oil, castor oil, rapeseed oil, and mineral oil.

[0033] In one embodiment of the present invention, the bactericide of the present invention is preferably prepared in the form of a suspension. When the bactericide of the present invention is prepared as a suspension, the content of each component can be: 5-25% of the bactericidal active ingredient composition, 2-8% of the dispersant, 2-8% of the wetting agent, 3-5% of the thickener, 3-5% of the antifreeze agent, 0.1-2% of the defoamer, and water to make up to 100%. The bactericidal active ingredient composition and its formulation are as described above.

[0034] In the preparation of the suspension, the adjuvants used are as described above. In a preferred embodiment, in the preparation of the suspension, the dispersant is preferably sodium naphthalenesulfonate formaldehyde condensate; the wetting agent is preferably trisiloxane polyoxyethylene ether, such as trisiloxane polyoxyethylene ether (8), and the weight ratio of sodium naphthalenesulfonate formaldehyde condensate to trisiloxane polyoxyethylene ether is 1:1-1.5, preferably 1:1.2-1.3, and more preferably 1:1.2. Through formulation screening experiments, the inventors have found that in the preparation of the suspension, the selection of the above-mentioned dispersant, wetting agent, and their proportions can significantly improve the suspension rate of the active ingredients thiamethoxam and Ipflufenoquin in the fungicide, which is very advantageous.

[0035] Another aspect of the present invention relates to the use of the bactericidal active ingredient composition or the fungicide of the present invention for the prevention and control of agricultural diseases. Specifically, the bactericidal active ingredient composition or the fungicide is brought into contact with the agricultural crop / plant or the pathogenic microorganism causing the agricultural disease before or after the occurrence of the agricultural disease.

[0036] Applicable crops / plants include: corn, rice, wheat, barley, rye, oats, sorghum, cotton, soybeans, mung beans, kidney beans, peanuts, buckwheat, sugar beets, rapeseed, sunflowers, sugarcane, tobacco, eggplant, tomato, potato, cucumber, pumpkin, zucchini, watermelon, winter melon, turnip, cabbage, kale, broccoli, apple, pear, peach, grape, plum, apricot, etc. Cucumbers and wheat are preferred.

[0037] Agricultural diseases that can be controlled include those commonly occurring on the crops, such as those caused by Oomycetes fungi (e.g., *Plasmodiophora*). Plasmodium phylum ), genus Arachycetes ( Physoderma ), Saprolegnia ( Saprolegnia ), genus Amygdalina ( Achlya ), Pythium ( Pythium Phytophthora ( ) Phytophthora ), genus *Peronospora* Downy mildew ), genus Pseudomonas ( Downy mildew ), Monopodial molds ( Plasmopara ), genus *Platycodon* ( Bremen ), Genus *Dactylogyrus* Sclerospora Diseases caused by fungi of the Zygomycota (such as Rhizopus spp.); diseases ... Rhizopus Mucor ( ) I am sick. ), genus *Pterocarya* Absidia ), genus *Cereus* ( Choanephora Diseases caused by fungi of the Ascomycota (such as the genus *Exocystis*). Taphrina ), Fungiales ( Eurotiales Aspergillus ( ) Aspergillus ), Penicillium ( Penicillium ), Powdery mildew ( Flower shop ), Monofilamentae ( Spheroid ), genus *Cyclocarya* ( Podosphere Powdery mildew ( Erysipelas ), genus *Cypripedium* Microsphere ), genus *Hylocereus* Hook ), genus *Symplocos* ( Phylactinia ), Long-beaked Shell ( Ceratocystis ), Gibberellins ( Gibberella ), Black Rot Species ( Waltz ), genus Cocci ( Mycosphaerella ), Coccidioides ( Guignard ), Black Star Fungus ( Ventura ), genus *Streptococcus* Monilinia asexual state is Necklaces ), Sclerotium genus ( Sclerotinia Diseases caused by fungi of the Basidiomycota (such as *Phyllostachys* genus); diseases caused by fungi of the Basidiomycota phylum (such as *Phyllostachys* genus). Powdery mildew ), genus *Russula* ( Gymnosporangium ), genus *Scattered rust* ( Melampsora ), genus Laminaria ( Phakopsora ), Ustilago maydis genus ( I burn. ), genus *Solanum* Tilletia ), Ustilago maydis genus ( Sphacelotheca ), genus *Septata* ( Septobasidium ), Exobasidiomycetes ( Exobasidium ), genus *Apocynum* Thanatephorus Armillaria mellea ( Armillaria Diseases caused by fungi (such as Rhizoctonia spp.) within the Deuteromycetes; diseases caused by fungi of the Deuteromycetes (such as Rhizoctonia spp.). Rhizoctonia ), Sclerotium genus ( Sclerotium ), genus *Platymonas* ( Necklaces ), genus *Pyrrosia* Oedipus ), Botrytis ( Botrytis ), genus *Pseudomonas* Ramularia Fusarium ( ) Fusarium ), Pyreus ( Firefly ), Penicillium ( Penicillium Aspergillus ( ) Aspergillus ), Verticillium ( Verticillium wilt Cercospora ( Cercospora Alternaria, Cladosporium Cladosporium ), Black Star Spore ( Sword ), genus *Helicobacter* ( Drechslera ), Helicobacterium genus ( Bipolar ), genus Helicobacter ( Exerohyl Diseases caused by fungi such as *Pseudomonas copaifolia*. Preferred for the control of cucumber downy mildew (*Pseudomonas copaifolia*). Pseudoperonospora cubensis )) and wheat scab (Fusarium graminearum ( Fusarium grasses )).

[0038] In the applications described in this invention, the application method, timing, and dosage of the bactericidal active ingredient composition or bactericide are all determined by those skilled in the art.

[0039] Another aspect of the present invention relates to a method for using the bactericidal active ingredient composition or the bactericide of the present invention to control agricultural diseases. Specifically, the bactericidal active ingredient composition or the bactericide is brought into contact with the agricultural crop / plant or the pathogenic microorganism causing the agricultural disease before or after the occurrence of the agricultural disease.

[0040] In the method described in this invention, the applicable crops / plants and the agricultural diseases used for prevention and control are the same as those described above.

[0041] Beneficial effects The fungicidal composition containing thiamethoxam and Ipflufenoquin described in this invention exhibits a significant synergistic effect in the prevention and control of agricultural diseases, especially downy mildew of cucumber and scab of wheat. Detailed Implementation

[0042] The present invention will be further described below with reference to embodiments, but the present invention is not limited thereto.

[0043] All reagents used in the following embodiments of the present invention can be obtained commercially available or in-house. Furthermore, unless otherwise specified, all percentages mentioned in the following embodiments are weight percentages.

[0044] I. Combined toxicity test 1. Combined virulence determination of thiamethoxam and Ipflufenoquin against cucumber downy mildew. Test pathogenic fungus: *Pseudomonas copaiformis* ( Pseudoperonospora cubensis The pathogen is the fungus that causes cucumber downy mildew. Fresh diseased cucumber leaves were collected, and downy mildew spores were washed off the underside of the leaves with distilled water at 4°C. A suspension was prepared (concentration approximately 1×10⁻⁶ spores per milliliter). 6 (1 spore), store at 4℃ for later use.

[0045] Test reagents: Thiamethoxam technical grade, Ipflufenoquin technical grade. Prepare stock solutions of the reagents, then mix them according to the predetermined proportions in Table 1, and prepare 5 concentration gradients with an aqueous solution containing 1% Tween-80 for later use.

[0046] Experimental Methods: Cucumber seedlings at the 4-6 leaf stage were selected and grown in pots in the laboratory. Fresh spore suspension was sprayed onto the underside of the leaves, with 6 pots per treatment and 2 seedlings per pot. 24 hours after inoculation, the solution was sprayed evenly onto both sides of the leaves until completely moistened, and allowed to air dry naturally. A 1% Tween-80 aqueous solution was used as a control. The seedlings were then placed in alternating light / dark conditions for 12 hours each, at a temperature of 20±1℃ and a relative humidity of 90%. Each treatment was replicated three times. After 7 days, the inoculated leaves were graded based on the disease incidence in the blank control. The grading method was as follows: Level 0: No disease; Grade 1: The area of ​​lesions accounts for less than 5% of the total leaf area; Grade 3: The lesion area accounts for 6% to 10% of the total leaf area; Level 5: The lesion area accounts for 11%-25% of the total leaf area; Level 7: The lesion area accounts for 26%-50% of the total leaf area; Level 9: The lesion area accounts for more than 50% of the total leaf area.

[0047] Based on the survey results, the disease index and prevention and control effects of each treatment were calculated:

[0048]

[0049] Regression analysis was performed based on the logarithmic values ​​of each drug concentration and the corresponding efficacy probability values ​​to calculate the EC50 of each drug. 50 Then, following Sun Yunpei's method, the EC values ​​for each treatment were measured. 50 Convert to Actual Toxicity Index (ATI); calculate the Theoretical Toxicity Index (TTI) based on the formulation ratio of the mixture, and calculate the Co-toxicity Coefficient (CTC) of the mixture according to the following formula. The results are shown in Table 1 below.

[0050]

[0051]

[0052] =

[0053]

[0054]

[0055] If the co-toxicity coefficient is greater than 120, it indicates a synergistic effect; if the co-toxicity coefficient is less than 80, it indicates an antagonistic effect; if the co-toxicity coefficient is between 80 and 120, it indicates an additive effect.

[0056] Table 1 Results of Indoor Toxicity Measurement

[0057] Table 1 shows that thiamethoxam and Ipflufenoquin have a synergistic effect on cucumber downy mildew in the range of 36:1 to 1:36, and the synergistic effect is more obvious in the range of 9:1 to 1:9. In particular, the synergistic effect is most obvious in the range of 4.5:1 to 1:4.5, which is the most preferred range of the invention.

[0058] 2. Combined virulence determination of thiamethoxam and Ipflufenoquin against Fusarium graminearum, the causal agent of wheat blight. Tested pathogenic fungus: Fusarium graminearum ( Fusarium gramineae This bacterium is the pathogen that causes wheat scab.

[0059] Test reagents: Thiamethoxam technical grade, Ipflufenoquin technical grade.

[0060] Experimental method: The toxicity of the agent to the pathogenic fungus was determined by the growth rate method, referring to the agricultural industry standard NY / T1156.2006.

[0061] Prepare a stock solution of the drug, then mix it according to the predetermined ratio in Table 2, and prepare five concentration gradients with an aqueous solution containing 1% Tween-80. Add the solutions to PDA medium at 45-50℃, with a drug-to-medium volume ratio of 1:9. Mix thoroughly and pour into sterile petri dishes. After cooling, form drug-containing mediums with different concentration gradients. Each concentration is repeated three times. An aqueous solution containing 1% Tween-80 is used as a control. Then, under aseptic conditions, inoculate the pathogenic bacterial pellets into the center of the drug-containing medium. Incubate the petri dishes in an incubator at 25±1℃ and approximately 85% humidity. When the colony radius on the control plate reaches approximately 2 / 3 of the plate radius, measure the colony diameter of each treatment using the cross-cross method, and calculate the growth inhibition rate of each drug treatment on the pathogen using the following formula:

[0062] Then, regression analysis was performed based on the logarithmic values ​​of each agent concentration and the corresponding efficacy probability values ​​to calculate the EC50 of each agent. 50 The co-toxicity coefficient (CTC) of each mixture was calculated using the aforementioned Sun Yunpei method. The results are shown in Table 2 below.

[0063] Table 2 Results of Indoor Toxicity Measurement

[0064] Table 2 shows that thiamethoxam and Ipflufenoquin have a synergistic effect on wheat scab in the range of 36:1 to 1:36, and the synergistic effect is more obvious in the range of 9:1 to 1:9. In particular, the synergistic effect is most obvious in the range of 4.5:1 to 1:4.5, which is the most preferred range of the invention.

[0065] II. Formulation Examples Example 1 Thiamethoxam 1%, Ipflufenoquin 9%, nonylphenol polyoxyethylene ether (10) 3%, calcium dodecylbenzenesulfonate 4%, carboxymethyl cellulose 4%, ethylene glycol 5%, n-octanol 0.5%, water balance.

[0066] The preparation method is as follows: The prescribed amounts of thiamethoxam, Ipflufenoquin, nonylphenol polyoxyethylene ether (10), calcium dodecylbenzenesulfonate, n-octanol and some water are added to a shear mill and dispersed and sheared at 1500 r / min for 30 min. Then, the mixture is transferred to a sand mill, zirconia beads are added and the mixture is ground at 500 r / min for 3 h. Finally, the prescribed amounts of carboxymethyl cellulose, ethylene glycol and the remaining water are added and mixed evenly to obtain the suspension.

[0067] Example 2 Thiamethoxam 2%, Ipflufenoquin 9%, sodium p-hydroxyphenyl lignin sulfonate 4%, siloxane polyoxyethylene ether (8) 4%, gum arabic 4%, propylene glycol 5%, silicone 0.5%, water balance.

[0068] The preparation method is the same as in Example 1.

[0069] Example 3 Thiamethoxam 9%, Ipflufenoquin 2%, sodium naphthalenesulfonic acid formaldehyde condensate 4%, triphenylethylphenol polyoxyethylene ether (15) 3%, xanthan gum 4%, ethylene glycol 5%, silicone 0.5%, water balance.

[0070] The preparation method is the same as in Example 1.

[0071] Example 4 Thiamethoxam 6%, Ipflufenoquin 6%, sodium naphthalenesulfonic acid formaldehyde condensate 3%, trisiloxane polyoxyethylene ether (8) 3.6%, magnesium aluminum silicate 4%, propylene glycol 5%, n-octanol 0.5%, water balance.

[0072] The preparation method is the same as in Example 1.

[0073] Example 5 Thiamethoxam 9%, Ipflufenoquin 1%, sodium naphthalenesulfonic acid formaldehyde condensate 5%, trisiloxane polyoxyethylene ether (8) 2%, magnesium aluminum silicate 4%, propylene glycol 5%, n-octanol 0.5%, water balance.

[0074] The preparation method is the same as in Example 1.

[0075] Then, the suspension rate of the prepared suspensions from Examples 1-5 was determined. The method for determining the suspension rate was as follows: 1 mL of the prepared suspension sample was added to a beaker containing 50 mL of standard hard water (342 mg / L) and stirred with a glass rod. Then, the entire sample was washed into a 250 mL graduated cylinder with standard hard water and diluted to the mark. The stopper was then placed on the cylinder, and the cylinder was inverted 30 times within 1 minute, with the bottom of the cylinder as the axis. The stopper was then opened, and the cylinder was placed vertically in a constant temperature water bath at 30°C for 30 minutes. Then, 9 / 10 (225 mL) of the contents was removed using a pipette. During the aspiration, the pipette was moved down the inner wall of the graduated cylinder as the liquid level dropped to avoid disturbing the lower sediment. The content of the active ingredient in the sample and the 25 mL of suspension remaining at the bottom of the graduated cylinder was determined. The experiment was repeated in triplicate. The suspension rate of the sample was calculated according to the following formula: Suspension rate = [(m1-m2) / m1] × (10 / 9) In the formula: m1 represents the mass of the effective component in the suspension sample; m2 represents the mass of the active ingredient remaining in the 25 mL suspension at the bottom of the graduated cylinder; The results are shown in Table 3 below.

[0076] Table 3 Results of suspension rate determination

[0077] As can be seen from the results in Table 3, the suspension rates of the suspending agents prepared in this invention are all greater than 80%. However, in Examples 4-5, when sodium naphthalenesulfonate formaldehyde condensate was used as the dispersant and trisiloxane polyoxyethylene ether was used as the wetting agent, the suspension rates of thiamethoxam and Ipflufenoquin were both greater than 90%. Furthermore, in Example 4, when the ratio of sodium naphthalenesulfonate formaldehyde condensate to trisiloxane polyoxyethylene ether was 3:3.6 (i.e., 1:1.2), the suspension rates of thiamethoxam and Ipflufenoquin were both greater than 95%, which is the most preferred embodiment of the invention.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A bactericidal active ingredient composition, characterized in that, The bactericidal active ingredient composition contains two active ingredients, thiamethoxam and Ipflufenoquin, with a weight ratio of thiamethoxam to Ipflufenoquin of 36:1 to 1:

36.

2. The bactericidal active ingredient composition according to claim 1, characterized in that, The weight ratio of thiamethoxam to Ipflufenoquin is 18:1 to 1:

18.

3. A bactericide, characterized in that, The fungicide comprises the fungicide active ingredient composition according to any one of claims 1-2, and agriculturally acceptable adjuvants and / or carriers.

4. The bactericide according to claim 3, characterized in that, The total weight percentage of active ingredients in the bactericide is 1-80%.

5. The bactericide according to claim 3, characterized in that, The agriculturally acceptable adjuvants and / or carriers include one or more of the following: solvents, cosolvents, dispersants, wetting agents, emulsifiers, thickeners, binders, antifreeze agents, defoamers, stabilizers, disintegrants, and fillers.

6. The bactericide according to claim 3, characterized in that, The bactericide can be formulated as one or more of the following: emulsifiable concentrate, wettable powder, water-dispersible granules, suspension concentrate, microemulsion, and water-in-oil emulsion.

7. The bactericide according to claim 6, characterized in that, The bactericide can be formulated as a suspension.

8. The use of the bactericidal active ingredient composition according to any one of claims 1-2 or the bactericide according to any one of claims 3-7 for the prevention and control of agricultural diseases, characterized in that, Before or after an agricultural disease occurs, the fungicide composition or the fungicide is brought into contact with the agricultural crop / plant or the pathogenic microorganism that causes the agricultural disease.

9. A method for controlling agricultural diseases using the bactericidal active ingredient composition according to any one of claims 1-2 or the bactericide according to any one of claims 3-7, characterized in that, Before or after an agricultural disease occurs, the fungicide composition or the fungicide is brought into contact with the agricultural crop / plant or the pathogenic microorganism that causes the agricultural disease.

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