Triene mycin photoaffinity probe, preparation method and application

The preparation method of trienemycin photoaffinity probe has solved the problem of low efficiency in traditional separation processes, and achieved high-yield preparation of intermediates and identification of trienemycin targets, thus meeting the needs of scientific research and the market.

CN122255113APending Publication Date: 2026-06-23NORTHWEST A & F UNIV +1
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Patent Information

Application Number
CN202610279022.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-09
Publication Date
2026-06-23

AI Technical Summary

Technical Problem

Existing technologies are insufficient for the efficient and large-scale production of trienemycin. Traditional separation processes are costly and have low synthesis efficiency, failing to meet the needs of scientific research and the market.

Method used

The preparation method of trienemycin photoaffinity probes includes using Trienomycinol as a substrate, reacting it with iodinated bispyridine for substitution, then removing the propionic protection, selectively esterifying the negative probe with Fmoc-D-alanine and condensing it with cyclohexane, and identifying the target by combining limited enzyme digestion mass spectrometry (LiP-MS).

Benefits of technology

The yield of intermediate preparation was improved, the preparation time was shortened, and the direct binding target of trienemycin was successfully identified, with high reliability of the results.

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Abstract

The invention discloses a trienomycin photoaffinity probe as well as a preparation method and application thereof, and the preparation method comprises the following steps: taking Trienomycin as a substrate, carrying out substitution reaction on Trienomycin and iodo-diaziridine, and then removing propylidene protection to obtain a corresponding photoaffinity negative probe; the method comprises the following steps: carrying out selective esterification on Fmoc-D-alanine and a negative probe 11-site hydroxyl group, then removing an Fmoc protecting group on nitrogen, and carrying out condensation with cyclohexanecarboxylic acid to obtain a corresponding photoaffinity positive probe trienamycin protein target, and comprises the following steps: (1) enriching an intermediate Trienomycin and synthesizing a trienamycin photoaffinity positive probe; and (2) completing target identification of the trienamycin through the photoaffinity probe, and performing molecular biology research on the target. The separated trienamycin intermediate, the synthesized trienamycin probe derivative and the identified trienamycin protein target have the advantages of high originality, high yield and the like.
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