Nucleic acid extraction apparatus

Abstract

The application discloses a nucleic acid extraction device, which comprises a bin body, a reagent tube carrier and an ultrasonic mechanism. The inside of the bin body is formed with a moving area and a mounting area which are distributed transversely adjacent to each other. The reagent tube carrier is arranged in the moving area and can move in the longitudinal direction. The reagent tube carrier is formed with a containing space for containing a reagent tube. A side wall on the lateral side of the containing space is formed with a probe entrance. The ultrasonic mechanism is arranged in the mounting area in a retractable mode. In the extended state, the ultrasonic mechanism can pass through the probe entrance and extend into the containing space. The extraction mechanism is arranged in the mounting area and located on the longitudinal side of the ultrasonic mechanism. The nucleic acid extraction device has simple structure, reasonable and compact part layout, and is conducive to miniaturization design.

Description

Technical Field

[0001] The field of nucleic acid extraction technology specifically relates to a nucleic acid extraction device. Background Technology

[0002] Nucleic acid extraction equipment (such as nucleic acid extractors) belongs to the field of molecular detection equipment and is widely used in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbiology testing, food safety testing, animal husbandry, and molecular biology research. However, the internal component settings and layout of existing nucleic acid extraction equipment are not reasonable enough, resulting in a large size of nucleic acid extraction equipment, which in turn requires a lot of space and is inconvenient to transport or place directly in a biosafety cabinet. Summary of the Invention

[0003] The purpose of this application is to provide a nucleic acid extraction device with a simple structure, reasonable and compact component layout, which is conducive to miniaturization design.

[0004] To achieve the above objectives, this application provides a nucleic acid extraction device, which includes:

[0005] The warehouse body has horizontally adjacent moving areas and installation areas inside.

[0006] A reagent tube carrier is set in the movable area and can move longitudinally. The reagent tube carrier has a receiving space for accommodating reagent tubes. A probe inlet and outlet are formed on the side wall of the receiving space on the lateral side.

[0007] The ultrasonic mechanism is telescopically mounted in the installation area, and can extend through the probe inlet and outlet and into the receiving space in its extended state.

[0008] The extraction mechanism is located in the installation area and on the longitudinal side of the ultrasonic mechanism.

[0009] In embodiments of this application, the extraction mechanism includes:

[0010] The first driving component is located in the installation area and distributed longitudinally;

[0011] The magnetic rod sleeve assembly is vertically movable in the moving area and is drivenly connected to the first driving member;

[0012] The second driving member is disposed above the first driving member and distributed longitudinally, and in the transverse direction, the second driving member overlaps with the first driving member;

[0013] The magnetic rod is vertically movable in the moving area. The magnetic rod is driven to connect with the second driving member and can partially extend into the magnetic rod sleeve assembly or separate from the magnetic rod sleeve assembly under the driving action of the second driving member.

[0014] In embodiments of this application, the extraction mechanism further includes:

[0015] The upright plate is set vertically in the installation area, with the first driving component set at the bottom end of the upright plate and the second driving component set at the top end of the upright plate.

[0016] The first synchronous belt assembly is vertically arranged on one side of the upright plate and drivenly connected to the first driving component. The first synchronous belt of the first synchronous belt assembly is connected to the magnetic rod sleeve assembly.

[0017] The second synchronous belt assembly is vertically disposed on the side of the upright plate away from the first synchronous belt assembly and is driven and connected to the second driving member. The second synchronous belt of the second synchronous belt assembly is connected to the magnetic rod.

[0018] In an embodiment of this application, a first mounting space for mounting a tensioning mechanism is formed on the upright plate, and the first mounting space is located between the first driving member and the second driving member.

[0019] In embodiments of this application, the nucleic acid extraction device further includes a heating element disposed at the bottom of the accommodating space for heating the reagents.

[0020] In an embodiment of this application, a receiving groove is formed on the heating element, which is recessed from top to bottom and is used to accommodate a portion of the reagent tube.

[0021] In embodiments of this application, the heating element includes a base portion and a stop portion, a receiving groove is formed on the base portion, and the stop portion is disposed on the top of the base portion and is capable of applying a lateral stopping force to the reagent tube.

[0022] In embodiments of this application, the nucleic acid extraction device further includes a clamping mechanism disposed on a reagent tube carrier and used to clamp the reagent tubes.

[0023] In embodiments of this application, the ultrasound mechanism includes:

[0024] The probe mounting base is set in the installation area and located on the longitudinal side of the extraction mechanism;

[0025] An ultrasonic probe is used to output ultrasonic waves to the reagent in the reagent tube. The ultrasonic probe is laterally movable and is mounted on the probe mounting base and faces the reagent tube carrier.

[0026] The drive connecting rod passes through the probe mounting base and forms a threaded connection with the probe mounting base;

[0027] The third driving component is driven and connected to the driving connecting rod.

[0028] In embodiments of this application, the ultrasonic mechanism further includes an elastic buffer assembly disposed on the probe mounting base for shock absorption of the ultrasonic probe.

[0029] As shown in the above technical solution, the nucleic acid extraction device includes a chamber, a reagent tube carrier, an ultrasonic mechanism, and an extraction mechanism. The reagent tube carrier is located in the movable area and can move longitudinally. The reagent tube carrier has a receiving space for accommodating reagent tubes, and a probe inlet / outlet is formed on the side wall of the receiving space on its lateral side. The ultrasonic mechanism is retractably located in the installation area, allowing it to extend through the probe inlet / outlet and into the receiving space in its extended state. The extraction mechanism is located in the installation area and on the longitudinal side of the ultrasonic mechanism. This nucleic acid extraction device has a simple structure. Because the ultrasonic mechanism and the extraction mechanism are located on the same lateral side of the reagent tube carrier, and the extraction mechanism is located on the longitudinal side of the ultrasonic mechanism, it is beneficial to reduce the overall width of the nucleic acid extraction device. This allows for a more compact layout of the components within the chamber, reducing the size of the nucleic acid extraction device and facilitating its miniaturization design. Consequently, the nucleic acid extraction device can be directly placed in a biosafety cabinet.

[0030] Other features and advantages of the embodiments of this application will be described in detail in the following detailed description section. Attached Figure Description

[0031] The accompanying drawings are provided to further illustrate the embodiments of this application and form part of the specification. They are used together with the following detailed description to explain the embodiments of this application, but do not constitute a limitation on the embodiments of this application. Those skilled in the art can obtain other drawings based on the structures shown in these drawings without any inventive effort. In the drawings:

[0032] Figure 1 This is a schematic diagram of the overall structure of the nucleic acid extraction device in the embodiments of this application;

[0033] Figure 2 This is a first-view structural schematic diagram of the reagent tube carrier in an embodiment of this application;

[0034] Figure 3 This is a second-view structural schematic diagram of the reagent tube carrier in an embodiment of this application;

[0035] Figure 4 This is a partial structural diagram of the reagent tube carrier in an embodiment of this application;

[0036] Figure 5 This is a schematic diagram of the ultrasonic mechanism and reagent tube carrier in the embodiments of this application;

[0037] Figure 6 This is a partial schematic diagram of the ultrasonic mechanism in an embodiment of this application;

[0038] Figure 7 This is a schematic diagram of the probe mounting base in an embodiment of this application;

[0039] Figure 8 This is a first-view structural diagram of the extraction mechanism in an embodiment of this application;

[0040] Figure 9 This is a second-view structural diagram of the extraction mechanism in an embodiment of this application;

[0041] Figure 10 This is a third-view structural diagram of the extraction mechanism in the embodiments of this application;

[0042] Figure 11 This is a schematic diagram of the magnetic rod sleeve mounting component in the embodiments of this application;

[0043] Figure 12 This is a schematic diagram of the installation of the magnetic rod in an embodiment of this application.

[0044] Explanation of reference numerals in the attached figures

[0045] 1-Moving area; 2-Installation area; 3-Reagent tube carrier; 301-Accommodation space; 302-Probe inlet / outlet; 303-First clearance notch; 304-Second clearance notch; 305-First partition; 306-Second partition; 307-Guide wall; 4-Reagent tube; 401-Cylindrical part; 402-Arcted part; 5-Ultrasonic mechanism; 501-Probe mounting base; 5011-Second installation space; 5012-Installation groove; 5013-Fifth connection part; 5014-Third installation part; 502-Ultrasonic probe; 5021-Second arc-shaped wall; 503- 504-Third driving component; 505-Elastic buffer assembly; 5051-First connecting rod; 5052-Connecting plate; 5053-Third elastic component; 6-Extraction mechanism; 601-First driving component; 602-Magnetic rod sleeve assembly; 6021-Magnetic rod sleeve mounting component; 6022-Insertion hole; 6023-Magnetic rod sleeve; 6024-Annular groove; 603-Second driving component; 604-Magnetic rod; 6041-Guide part; 605-Upright plate; 6051-First mounting space; 606-First synchronous belt assembly; 6061-First synchronous belt; 6062-First 6063 - First driven pulley; 607 - Second synchronous belt assembly; 6071 - Second synchronous belt; 6072 - Second driving pulley; 6073 - Second driven pulley; 608 - Second guide rail; 609 - First slider; 6091 - First connecting part; 6092 - First mounting part; 6093 - Third connecting part; 610 - Second slider; 6101 - Second connecting part; 6102 - Second mounting part; 6103 - Fourth connecting part; 611 - First reset assembly; 6111 - First baffle; 6112 - First sensing element; 612 - Side plate; 613 - Second reset assembly Position component; 6131-Second baffle; 6132-Second sensing element; 7-Heating element; 701-Receiving groove; 702-Base part; 703-Stop part; 704-First arc-shaped wall; 8-Clamping mechanism; 801-First clamping element; 802-Second clamping element; 803-First connecting element; 804-Second connecting element; 9-First guide rail; 10-First heat insulation element; 11-Drive mechanism; 1101-Fourth driving element; 1102-Lead screw; 1103-Threaded connecting block; 12-Second heat insulation element; 13-Leak-proof cover plate; 14-Control circuit board; 15-Pushing part. Detailed Implementation

[0046] The specific embodiments of this application will be described in detail below with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are for illustration and explanation only and are not intended to limit this application.

[0047] Embodiments of this application provide a nucleic acid extraction device, such as... Figures 1-3 and Figures 8-10 As shown, the nucleic acid extraction device includes:

[0048] The warehouse body has a horizontally adjacent moving area 1 and an installation area 2 inside.

[0049] The reagent tube carrier 3 is set in the moving area 1 and can move longitudinally. The reagent tube carrier 3 has a receiving space 301 for accommodating the reagent tube 4. The receiving space 301 has a probe inlet / outlet 302 formed on the side wall of the transverse side.

[0050] The ultrasonic mechanism 5 is telescopically mounted in the installation area 2. The ultrasonic mechanism 5 can pass through the probe inlet / outlet 302 and extend into the receiving space 301 in an extended state.

[0051] Extraction mechanism 6 is set in installation area 2 and located on the longitudinal side of ultrasonic mechanism 5.

[0052] Specifically, the nucleic acid extraction device in this embodiment can be a nucleic acid extractor. The accommodating space 301 is formed by a downward indentation from the top of the reagent tube carrier 3. There are multiple reagent tubes 4, each containing different types of solutions (such as a mixture of lysis buffer and sample, washing solution, buffer solution, etc.). Before the ultrasonic mechanism 5 performs the ultrasonic lysis operation, the reagent tube carrier 3 is controlled to perform a first movement operation to move the reagent tube carrier 3 to a preset lysis position. Then, the ultrasonic mechanism 5 is controlled to extend until part of the ultrasonic mechanism 5 passes through the probe inlet / outlet 302 and contacts the reagent tube 4. After the ultrasonic mechanism 5 activates its ultrasonic function, it outputs ultrasonic waves to the reagent tube 4. The reagent tube 4 then transfers the energy of the ultrasonic waves to the reagents in the reagent tube 4 (such as a mixture of lysis buffer and sample, where the sample contains cells). The high-frequency sound wave energy of the ultrasonic waves can break the cells so that the intracellular substances (such as nucleic acids) flow out, laying the foundation for subsequent nucleic acid extraction.

[0053] After the ultrasonic lysis operation is completed, the ultrasonic mechanism 5 controls the reagent tube carrier 3 to perform a second movement operation, so that the reagent tube 4 containing the lysed nucleic acid moves to the preset extraction position. Then, the extraction mechanism 6 extracts the lysed nucleic acid from the original reagent tube 4 (i.e., the reagent tube 4 that has undergone ultrasonic lysis operation). After the extraction mechanism 6 extracts the nucleic acid, it performs an upward movement operation. Then, the reagent tube carrier 3 is controlled to perform a third movement operation, so that the reagent tube 4 containing the washing solution moves to the preset extraction position. After the reagent tube carrier 3 performs the third movement operation, the extraction mechanism 6 is controlled to perform a downward movement operation, so that the extracted nucleic acid is transferred to the reagent tube 4 containing the washing solution for washing.

[0054] The nucleic acid extraction device in this embodiment has a simple structure. Since the ultrasonic mechanism 5 and the extraction mechanism 6 are located on the same lateral side of the reagent tube carrier 3, and the extraction mechanism 6 is located on the longitudinal side of the ultrasonic mechanism 5, it is beneficial to reduce the overall width of the nucleic acid extraction device, making the components of the nucleic acid extraction device more compactly arranged inside the chamber, which is beneficial to reduce the volume of the nucleic acid extraction device and to realize the miniaturization design of the nucleic acid extraction device. As a result, the nucleic acid extraction device can be directly placed in the biosafety cabinet.

[0055] In one embodiment of this application, such as Figures 8-10 As shown, the extraction mechanism 6 includes:

[0056] The first driving component 601 is disposed in the installation area 2 and distributed along the longitudinal direction;

[0057] The magnetic rod sleeve assembly 602 is vertically movable in the moving area 1 and is drivenly connected to the first driving member 601;

[0058] The second driving member 603 is disposed above the first driving member 601 and distributed longitudinally, and in the transverse direction, the second driving member 603 overlaps with the first driving member 601.

[0059] The magnetic rod 604 is vertically movable in the moving area 1. The magnetic rod 604 is drivenly connected to the second driving member 603 and can partially extend into the magnetic rod sleeve assembly 602 or separate from the magnetic rod sleeve assembly 602 under the driving action of the second driving member 603.

[0060] Specifically, the installation area 2 and the moving area 1 extend longitudinally inside the chamber. The nucleic acid extraction device also includes a first guide rail 9, which is set in the moving area 1. The reagent tube holder 3 is movably set on the first guide rail 9. The reagent tube 4 containing the sample is also pre-placed with magnetic beads that can adsorb nucleic acids.

[0061] In this embodiment, both the first driving member 601 and the second driving member 603 can be stepper motors. Under the driving action of the first driving member 601, the magnetic rod sleeve assembly 602 moves up and down above the moving area 1; under the driving action of the second driving member 603, the magnetic rod 604 can move up and down above the moving area 1. By changing the respective movement speeds of the first driving member 601 and the second driving member 603, the magnetic rod sleeve assembly 602 and the magnetic rod 604 can move individually or together.

[0062] Before nucleic acid extraction, a portion of the magnetic rod sleeve assembly 602 is pre-inserted into the reagent tube 4 to be extracted. Then, the second driving component 603 is controlled to move, causing the magnetic rod 604 to move downwards until it reaches its designated position. After the magnetic rod 604 reaches its position, a portion of it extends into the magnetic rod sleeve assembly 602 to pick up the magnetic beads in the reagent tube 4. After the magnetic beads are picked up, the first driving component 601 and the second driving component 603 are controlled to rotate together, causing the magnetic rod 604 and the magnetic rod sleeve assembly 602 (on which magnetic beads are adsorbed, and on which nucleic acid is adsorbed) to move upwards at the same speed. After the magnetic rod sleeve assembly 602 detaches from the reagent tube 4 containing the adsorbed nucleic acid, the reagent tube moves upwards. The tube carrier 3 moves the reagent tube 4 containing the washing solution directly below the magnetic rod sleeve assembly 602. After the reagent tube carrier 3 is in place, the first drive member 601 and the second drive member 603 are controlled to rotate together again, so that the magnetic rod 604 and the magnetic rod sleeve assembly 602 (with magnetic beads adsorbed on them, and nucleic acid adsorbed on the magnetic beads) move downward at the same speed, thereby allowing the magnetic rod sleeve assembly 602 with magnetic beads adsorbed to extend into the reagent tube 4 below it. After the magnetic rod 604 and the magnetic rod sleeve assembly 602 move downward into place, the second drive member 603 is controlled to rotate, thereby causing the magnetic rod 604 to move upward and away from the magnetic rod sleeve assembly 602, so as to release the magnetic beads and clean the magnetic beads with nucleic acid adsorbed.

[0063] The extraction mechanism 6 in this embodiment has a simple structure. Since the first driving member 601 and the second driving member 603 are distributed vertically at intervals, and the second driving member 603 overlaps with the first driving member 601 in the horizontal direction, the overall width of the first driving member 601 and the second driving member 603 is consistent with the width of the first driving member 601 (or the width of the second driving member 603), which is beneficial to reducing the overall width of the extraction mechanism 6. Furthermore, since the first driving member 601 and the second driving member 603 are both arranged along the longitudinal direction, the width occupied by the first driving member 601 and the second driving member 603 inside the extraction mechanism 6 can be further reduced, thereby making the layout between the components of the extraction mechanism 6 more compact, which is beneficial to reducing the volume of the extraction mechanism 6 and the nucleic acid extraction device.

[0064] Furthermore, in this embodiment, the magnetic flux of the magnetic rod 604 is 5000 Gauss. The magnetic rod 604 with this magnetic flux can effectively adsorb the nano-sized magnetic beads in the reagent tube 4, thereby achieving the effect of highly extracting nucleic acids.

[0065] In one embodiment of this application, the extraction mechanism 6 further includes:

[0066] A vertical plate 605 is set vertically in the installation area 2. A first driving component 601 is set at the bottom end of the vertical plate 605, and a second driving component 603 is set at the top end of the vertical plate 605.

[0067] The first synchronous belt assembly 606 is vertically disposed on one side of the upright plate 605 and drivenly connected to the first driving member 601. The first synchronous belt 6061 of the first synchronous belt assembly 606 is connected to the magnetic rod sleeve assembly 602.

[0068] The second synchronous belt assembly 607 is vertically disposed on the side of the upright plate 605 away from the first synchronous belt assembly 606 and is drivenly connected to the second driving member 603. The second synchronous belt 6071 of the second synchronous belt assembly 607 is connected to the magnetic rod 604.

[0069] Specifically, the upright plate 605 has a first mounting hole and a second mounting hole located at the bottom and top ends, respectively. The second mounting hole is located directly above the first mounting hole. The first synchronous belt assembly 606 includes a first driving pulley 6062, a first driven pulley 6063, and a first synchronous belt 6061. The first driving pulley 6062 is disposed on the driving end of the first driving member 601 passing through the first mounting hole. The first driven pulleys 6063 are spaced above the first driving pulley 6062. The first synchronous belt 6061 is sleeved on the outside of the first driving pulley 6062 and the first driven pulley 6063. When the first driving member 601 rotates, it drives the first synchronous belt 6061. The rotation of the first synchronous belt 6061 causes the magnetic rod sleeve assembly 602 to move up and down. Similarly, the second synchronous belt assembly 607 includes a second driving wheel 6072, a second driven wheel 6073, and a second synchronous belt 6071. The second driving wheel 6072 is disposed on the driving end of the second driving member 603 passing through the second mounting hole. The second driven wheels 6073 are spaced above the second driving wheel 6072. The second synchronous belt 6071 is sleeved on the outside of the second driving wheel 6072 and the second driven wheel 6073. When the second driving member 603 rotates, it drives the second synchronous belt 6071 to rotate, thereby causing the magnetic rod 604 to move up and down. The arrangement of the first synchronous belt 6061 and the second synchronous belt 6071 can reduce the noise of the extraction mechanism 6 during use and effectively improve the user experience of the extraction mechanism 6 and the nucleic acid extractor.

[0070] Furthermore, the first synchronous belt assembly 606 and the second synchronous belt assembly 607 are located on both sides of the upright plate 605, the first synchronous belt assembly 606 is located below the second driving member 603, and the second synchronous belt assembly 607 is located above the first driving member 601. The above arrangement makes full use of the space below the second driving member 603 and further improves the compactness of the layout between the components of the extraction mechanism 6.

[0071] In one embodiment of this application, a first mounting space 6051 for mounting a tensioning mechanism is formed on the upright plate 605, and the first mounting space 6051 is located between the first driving member 601 and the second driving member 603.

[0072] Specifically, the nucleic acid extraction device also includes a tensioning mechanism for tensioning the first synchronous belt 6061 and / or the second synchronous belt 6071, which helps ensure the accuracy of the up-and-down movement of the magnetic rod 604 and the magnetic rod sleeve 6023. In this embodiment, the tensioning mechanism is located in the first installation space 6051 and between the first synchronous belt assembly 606 and the second synchronous belt assembly 607, further improving the utilization rate of the internal space of the extraction mechanism 6 and making the multiple components of the extraction mechanism 6 more compact.

[0073] In one embodiment of this application, the extraction mechanism 6 further includes:

[0074] The second guide rail 608 is vertically installed on the side wall of the upright plate 605 near the moving area 1;

[0075] The first slider 609 is movably disposed on the second guide rail 608. The first slider 609 has a first connecting part 6091 connected to the first synchronous belt 6061 and a first mounting part 6092 for mounting the magnetic rod sleeve assembly 602.

[0076] The second slider 610 is movably disposed on the second guide rail 608 and located above the first slider 609. The second slider 610 has a second connecting part 6101 connected to the second synchronous belt 6071 and a second mounting part 6102 for mounting the magnetic rod 604.

[0077] Specifically, the first slider 609 further includes a third connecting part 6093 vertically and movably disposed on the second guide rail 608. One end of the first connecting part 6091 is connected to the first synchronous belt 6061, and the other end of the first connecting part 6091 is connected to the third connecting part 6093. The first mounting part 6092 is flat and L-shaped with the third connecting part 6093. When the first driving member 601 rotates, it drives the first synchronous belt 6061 to rotate. The first synchronous belt 6061 then drives the first slider 609 to move up and down along the second guide rail 608 through the first connecting part 6091. The first slider 609 then drives the magnetic rod sleeve assembly 602 to move up and down synchronously. Similarly, the second slider 610 also includes a fourth connecting part 6103 vertically and movably disposed on the second guide rail 608. One end of the second connecting part 6101 is connected to the second synchronous belt 6071, and the other end of the second connecting part 6101 is connected to the fourth connecting part 6103. The second mounting part 6102 is flat and L-shaped with the fourth connecting part 6103. When the second driving member 603 rotates, it drives the second synchronous belt 6071 to rotate. The second synchronous belt 6071 then drives the second slider 610 to move up and down along the second guide rail 608 through the second connecting part 6101. The second slider 610 then drives the magnetic rod 604 to move up and down synchronously. The above-mentioned structure is simple and helps to reduce the production and manufacturing costs of the extraction mechanism 6 and the nucleic acid extraction equipment as a whole.

[0078] In one embodiment of this application, the magnetic rod sleeve assembly 602 includes:

[0079] A magnetic rod sleeve mounting part 6021 is provided on the first mounting part 6092, and an insertion hole 6022 for the magnetic rod 604 to pass through is formed on the magnetic rod sleeve mounting part 6021.

[0080] The magnetic rod sleeve 6023 is disposed in the reagent tube 4 and is detachably connected to the magnetic rod sleeve mounting part 6021.

[0081] Specifically, in this embodiment, the magnetic rod sleeve mounting component 6021 can be selected as a magnetic rod sleeve 6023 adapter and is detachably connected to the first mounting part 6092 via a bolt assembly. The bottom end of the magnetic rod sleeve mounting component 6021 passes downward through the first mounting part 6092. The interior of the magnetic rod sleeve 6023 forms a receiving cavity, and multiple protrusions are formed on the inner wall of the receiving cavity at circumferential intervals. An annular groove 6024 for receiving the multiple protrusions is formed on the outer peripheral wall of the magnetic rod sleeve mounting component 6021. The magnetic rod sleeve 6023 is pre-placed in the reagent tube 4, and the reagent... After the tube 4 moves into the interior of the nucleic acid extraction device along with the reagent tube carrier, the reagent tube 4 with the magnetic rod sleeve 6023 moves to directly below the magnetic rod sleeve mounting piece 6021. The first driving member 601 drives the first slider 609 to move the magnetic rod sleeve mounting piece 6021 downward. After the magnetic rod sleeve mounting piece 6021 moves downward into place, part of the magnetic rod sleeve mounting piece 6021 extends downward into the receiving cavity of the magnetic rod sleeve 6023, and the protrusion is engaged in the annular groove 6024. The connection between the magnetic rod sleeve 6023 and the magnetic rod sleeve mounting piece 6021 is achieved in the above manner.

[0082] In one embodiment of this application, the insertion hole 6022 is funnel-shaped, wider at the top and narrower at the bottom, and has a guide section at the top, which facilitates the magnetic rod 604 to pass through the magnetic rod sleeve mounting part 6021 quickly and smoothly, which helps to improve the smoothness of use of the extraction mechanism 6.

[0083] In one embodiment of this application, a guide portion 6041 is formed at the end of the magnetic rod 604 away from the second mounting portion 6102. The guide portion 6041 is in the shape of an inverted frustum, which helps to further improve the smoothness of the magnetic rod 604 passing down through the magnetic rod sleeve mounting member 6021.

[0084] In one embodiment of this application, the extraction mechanism 6 further includes a second reset component 613 for resetting the second drive member 603, the second reset component 613 including:

[0085] The second baffle 6131 is disposed on the second slider 610;

[0086] The second sensing element 6132 is disposed on the upright plate 605 and located on the same side as the first synchronous belt assembly 606, and is used to detect the second baffle 6131.

[0087] Specifically, the extraction mechanism 6 also includes a processor, which is communicatively connected to the second sensor 6132 and the second drive unit 603. The second baffle 6131 is disposed on the vertical sidewall of the second slider 610 near the first synchronous belt assembly 606. The second sensor 6132 can be a photoelectric sensor and is communicatively connected to the second drive unit 603. When the second slider 610 moves into position, the second sensor 6132 extends into the second detection groove of the second sensor 6132. The potential of the second sensor 6132 changes and sends the potential change to the processor. After receiving the change, the processor can determine that the second sensor 6132 has detected the second baffle 6131. ​​The processor then controls the second drive unit 603 to reset. The above settings ensure the accuracy of the movement of the second drive unit 603 and the magnetic rod 604, thereby ensuring the accuracy of the extraction mechanism 6 when performing the extraction operation.

[0088] In one embodiment of this application, the extraction mechanism 6 further includes a side plate 612 vertically disposed on the side of the upright plate 605 near the moving area 1, and a first reset assembly 611 for resetting the first driving member 601. The first reset assembly 611 includes:

[0089] The first baffle plate 6111 is disposed on the first slider 609 and located on the side of the first slider 609 near the side plate 612;

[0090] The first sensing element 6112 is disposed on the side plate 612 and is used to detect the first baffle 6111.

[0091] Specifically, the processor is communicatively connected to the first sensor 6112 and the first drive unit 601. The first baffle 6111 is disposed on the vertical sidewall of the first slider 609 near the side plate 612. The first sensor 6112 can be a photoelectric sensor and is communicatively connected to the first drive unit 601. When the first slider 609 moves into position, the first baffle 6111 extends into the first detection groove of the first sensor 6112. The potential of the first sensor 6112 changes and sends the potential change to the processor. After receiving the change, the processor can determine that the first sensor 6112 has detected the first baffle 6111. The processor then controls the first drive unit 601 to reset. The above settings ensure the accuracy of the movement of the first drive unit 601 and the magnetic rod sleeve assembly 602, and further ensure the accuracy of the extraction mechanism 6 when performing the extraction operation.

[0092] In one embodiment of this application, such as Figures 2-5 As shown, the nucleic acid extraction device also includes a heating element 7 disposed at the bottom of the accommodating space 301 for heating the reagents in the reagent tube 4.

[0093] Specifically, before nucleic acid extraction, the heating element 7 heats the reagent (a mixture of lysis buffer and sample) in the reagent tube 4, which helps the cells in the sample release nucleic acid more quickly, laying the foundation for subsequent nucleic acid extraction. Positioning the heating element 7 at the bottom of the accommodating space 301 achieves an integrated design of the reagent tube carrier 3 and the heating element 7, improving the space utilization of the accommodating space 301. It heats the reagent while reducing the overall space occupied by the reagent tube carrier 3 and the heating element 7, which helps to further reduce the size of the nucleic acid extraction equipment. Furthermore, in this embodiment, the heating element 7 can heat the reagent, improving the efficiency of ultrasonic lysis. That is, the nucleic acid extraction equipment in this embodiment can also use a lysis method that primarily uses ultrasonic lysis and secondarily uses heating lysis to release nucleic acid from cells, which is beneficial for improving the nucleic acid extraction effect. By adjusting the ultrasonic frequency, it is possible to prevent the large amount of heat generated by ultrasound from affecting the nucleic acid yield and integrity. The nucleic acid extraction equipment in this embodiment can also individually heat and lyse reagents suitable for separate heating to release nucleic acid from cells, which helps to further expand the applicability of cell-to-nucleic acid release.

[0094] In one embodiment of this application, a receiving groove 701 is formed on the heating element 7, which is recessed from top to bottom and is used to accommodate part of the reagent tube 4. After the reagent tube 4 is placed, the bottom of the reagent tube 4 falls into the receiving groove 701. The setting of the receiving groove 701 reduces the shaking of the reagent tube 4 inside the receiving space 301, improves the stability of the reagent tube 4 after placement, and is beneficial to improving the heating and nucleic acid extraction effect.

[0095] In one embodiment of this application, the heating element 7 includes a base portion 702 and a stop portion 703. A receiving groove 701 is formed on the base portion 702, and the stop portion 703 is disposed on the top of the base portion 702 and is capable of applying a lateral stopping force to the reagent tube 4.

[0096] Specifically, in this embodiment, the stop part 703 can apply a lateral stopping force to the reagent tube 4. The ultrasonic mechanism 5 contacts the reagent tube 4 from the side away from the stop part 703 and outputs ultrasonic waves. When the ultrasonic mechanism 5 outputs ultrasonic waves, the reagent tube 4 tends to move away from the ultrasonic mechanism 5 under the action of ultrasonic waves. In this case, the stop part 703 acts as a stop for the reagent tube 4. That is, the ultrasonic force and the lateral stopping force applied by the ultrasonic mechanism 5 are located on the opposite radial sides of the reagent tube 4. Therefore, the setting of the stop part 703 can further enhance the stability of the reagent tube 4 when it contacts the ultrasonic mechanism 5, which is beneficial to further improve the ultrasonic pyrolysis effect.

[0097] In one embodiment of this application, the stop portion 703 has a first arc-shaped wall 704 formed on the side facing the reagent tube 4, which can fit against the outer peripheral wall of the reagent tube 4. The first arc-shaped wall 704 can make the force on the reagent tube 4 more uniform when it comes into contact with the stop portion 703, and can avoid the reagent tube 4 from being damaged due to concentrated force.

[0098] In one embodiment of this application, the heating element 7 includes a heat conductor and a heating element (not shown in the figure). The heating element dissipates heat, and the heat conductor is connected to the heating element and transfers the heat to the reagent. The base portion 702 and the stop portion 703 are both formed on the heat conductor. Furthermore, in this embodiment, the heat conductor is made of aluminum alloy, which is inexpensive and has the advantage of fast heat conduction, effectively ensuring the heating effect of the heating element 7 on the reagent.

[0099] In one embodiment of this application, the nucleic acid extraction device further includes a first heat insulation member 10 embedded in the reagent tube carrier 3 and located below the heating member 7.

[0100] Specifically, in this embodiment, the reagent tube holder 3 can be made of aluminum alloy. The reagent tube holder 3 made of this material is lightweight and inexpensive. The first heat insulation element 10 can isolate the heat transfer from the heating element 7, preventing the heat from the heating element 7 from being transferred to the reagent tube holder 3 and causing damage to other components connected to the reagent tube holder 3, thus extending the service life of the nucleic acid extraction equipment. Furthermore, the height of the top surface of the first heat insulation element 10 is greater than or equal to the height of the bottom wall of the accommodating space 301. When the height of the top surface of the first heat insulation element 10 is greater than the height of the bottom wall of the accommodating space 301, the first heat insulation element 10 will not interfere with the reagent tube 4 located on one side of the heating element 7 (e.g., the side along the length of the heating element 7).

[0101] In one embodiment of this application, such as Figure 4 As shown, the nucleic acid extraction device also includes a second heat insulation member 12 disposed below the first heat insulation member 10 and used to push the first heat insulation member 10 upward. Specifically, in this embodiment, other components (such as control circuit boards) are also disposed below the second heat insulation member 12. The second heat insulation member 12 can further prevent the heat of the heating member 7 from being transferred downward and protect the components located below the second heat insulation member 12.

[0102] In one embodiment of this application, such as Figure 4As shown, the nucleic acid extraction device also includes a leak-proof cover 13 disposed below the second heat insulation member 12. Specifically, the control circuit board 14 is used to control the nucleic acid extraction device. The leak-proof cover 13 is disposed between the second heat insulation member 12 and the control circuit board 14. The leak-proof cover 13 can prevent the solution that accidentally leaks from the reagent tube 4 from dripping down through the bottom wall of the receiving space 301 onto the control circuit board 14, thus providing good protection for the control circuit board 14 and further improving the reliability of the nucleic acid extraction device.

[0103] Furthermore, the nucleic acid extraction equipment also includes a door that can be flipped up and located at the outlet, such as... Figure 3 As shown, the reagent tube carrier 3 is also provided with a pushing part 15 on the vertical side wall facing the compartment door for applying a pushing force to the compartment door. Specifically, the pushing part 15 protrudes from the vertical side wall of the reagent tube carrier 3 facing the compartment door in the direction facing the compartment door. When the reagent tube carrier 3 moves out of the compartment with the reagent tube 4, the pushing part 15 contacts the compartment door before the reagent tube carrier 3 and applies a pushing force to the compartment door as the reagent tube carrier 3 continues to move. Under the action of the above-mentioned pushing force, the compartment door can be flipped relative to the compartment body and exposed to the compartment opening, thereby allowing the reagent tube carrier 3 and the reagent tube 4 to exit the compartment smoothly, avoiding the situation where the compartment door is difficult to push open.

[0104] In one embodiment of this application, the nucleic acid extraction device further includes a first guide rail 9, which is disposed inside the chamber, and a reagent tube carrier 3 is movably disposed on the first guide rail 9 and can be moved to the outside of the chamber.

[0105] Specifically, the chamber has an outlet, and the first guide rail 9 is located inside the chamber (not shown in the figure) and aligned with the outlet. The nucleic acid extraction device also includes a drive mechanism 11 that is driven and connected to the reagent tube carrier 3. Under the drive of the drive mechanism 11, the reagent tube carrier 3 can pass through the outlet and move to the outside of the chamber. The above arrangement allows the reagent tube carrier 3 to be moved to the outside of the chamber when it is necessary to place or replace the reagent tube 4. In this case, since the reagent tube carrier 3 is stably and reliably connected to the drive mechanism 11, it will not tip over. This makes it convenient for the operator to directly put the reagent tube 4 into the receiving space 301 or take the reagent tube 4 out of the receiving space 301 without having to first move the reagent tube carrier 3 out of the chamber and then take the reagent tube 4 off the reagent tube carrier 3. This improves the convenience of installing or replacing the reagent tube 4 and helps to improve the efficiency of nucleic acid extraction.

[0106] In one embodiment of this application, such as Figure 8As shown, the drive mechanism 11 includes a fourth drive member 1101, a lead screw 1102, and a threaded connecting block 1103. The fourth drive member 1101 is arranged longitudinally in the installation area 2 and located between the installation area 2 and the reagent tube carrier 3. The lead screw 1102 is arranged longitudinally and is driven and connected to the drive output end of the fourth drive member 1101. One lateral end of the threaded connecting block 1103 is sleeved on the lead screw 1102 and forms a threaded connection with the lead screw 1102. The reagent tube carrier 3 is connected to the other lateral end of the threaded connecting block 1103.

[0107] Specifically, in this embodiment, the fourth driving component 1101 can be a motor. When the fourth driving component 1101 rotates, it drives the lead screw 1102 to rotate. The threaded connecting block 1103 moves the reagent tube carrier 3 along the axial direction of the lead screw 1102, thereby driving the reagent tube carrier 3 to move on the first guide rail 9. The driving mechanism 11 in this embodiment has a simple structure, makes full use of the space inside the chamber, and has a reasonable and compact layout, which is conducive to further reducing the overall volume of the nucleic acid extraction equipment.

[0108] In one embodiment of this application, the nucleic acid extraction device further includes;

[0109] Temperature sensing element, used to detect the temperature of the heat conductor of heating element 7;

[0110] A temperature protection switch is used to control the heating element to stop heating when the temperature of the heating element 7 exceeds the preset temperature.

[0111] Specifically, the temperature detection element (not shown in the figure) can be a temperature sensor and is located inside the heat conductor of the heating element 7. The temperature detection element helps improve the accuracy of temperature control of the liquid to be heated and improves the efficiency of nucleic acid lysis. The temperature protection switch (not shown in the figure) is located on the heating element of the heating element 7 and is communicatively connected to the heating element. It can directly detect the temperature of the heating element and control the heating element to stop heating if the temperature of the heating element exceeds the preset temperature, thus forming overheat protection to prevent the reagent tube 4 from being damaged due to excessive temperature and to prevent the solution to be heated from being volatilized and causing environmental pollution due to excessive temperature.

[0112] In one embodiment of this application, such as Figures 2-5As shown, the nucleic acid extraction device also includes a clamping mechanism 8 disposed on the reagent tube carrier 3 and used to clamp the reagent tube 4. The clamping mechanism 8 includes a first clamping member 801, a second clamping member 802, a connecting assembly, and an elastic component disposed on the connecting assembly. The first clamping member 801 is laterally movable and disposed on the reagent tube carrier 3 and located on one side of the receiving space 301. The second clamping member 802 is laterally movable and disposed on the reagent tube carrier 3 and located on the other side of the receiving space 301. The connecting assembly is laterally movable and disposed on the reagent tube carrier 3, with its two ends connected to the first clamping member 801 and the second clamping member 802 respectively. One end of the elastic component abuts against the first clamping member 801, and the other end of the elastic component abuts against the reagent tube carrier 3.

[0113] When installing reagent tube 4, the operator first places the bottom of reagent tube 4 into the receiving space 301. Then, the operator continues to apply a downward force to reagent tube 4. Under the action of the above force, reagent tube 4 continues to move downward. During the downward movement of reagent tube 4, reagent tube 4 will exert a pushing force on the second clamping member 802 away from the direction of the first clamping member 801. The first clamping member 801, the second clamping member 802, and the connecting assembly as a whole move towards the direction of the second clamping member 802, and the elastic component is compressed. After the reagent tube 4 moves downward into place, under the elastic force of the elastic component, the second clamping member 802 continues to apply a pushing force to reagent tube 4. The second clamping member 802 and the vertical sidewall of the receiving space 301 away from the second clamping member 802 work together to clamp reagent tube 4 to ensure the stability of reagent tube 4 after installation, so that reagent tube 4 is in a stable state during ultrasonic lysis, ensuring smooth ultrasonic lysis operation, and improving the effect and efficiency of ultrasonic lysis.

[0114] When reagent tube 4 needs to be removed, the operator applies a pushing force towards the second clamp 802 to the first clamp 801, further compressing the elastic component to move the first clamp 801, the second clamp 802, and the connecting component as a whole towards the direction of the second clamp 802. This releases the clamping effect of the second clamp 802 and the vertical sidewall of the accommodating space 301 away from the second clamp 802 on the reagent tube 4. Then, an upward force is applied to the reagent tube 4 to remove it, making it easy to remove and improving the convenience and reliability of the nucleic acid extraction equipment.

[0115] The nucleic acid extraction device in this embodiment has a simple structure. The clamping mechanism 8 can ensure that the reagent tube 4 is in a stable state during the ultrasonic lysis process, thereby improving the effect and efficiency of ultrasonic lysis. It can also make the reagent tube 4 easy and quick to remove, thus improving the reliability, ease of use and user experience of the nucleic acid extraction device.

[0116] In one embodiment of this application, a first partition 305 and a second partition 306 are formed on the reagent tube carrier 3 at its longitudinal ends and between the first clamping member 801 and the second clamping member 802. The connecting assembly includes a first connector 803 and a second connector 804. The first connector 803 is movably inserted through the first partition 305 and its two ends are respectively connected to the first clamping member 801 and the second clamping member 802. The second connector 804 is movably inserted through the second partition 306 and its two ends are respectively connected to the first clamping member 801 and the second clamping member 802. The elastic assembly includes a first elastic member and a second elastic member. The first elastic member is sleeved on the outside of the first connector 803 and its two ends abut against the first clamping member 801 and the first partition 305, respectively. The second elastic member is sleeved on the outside of the second connector 804 and its two ends abut against the first clamping member 801 and the second partition 306, respectively.

[0117] Specifically, a first clearance notch 303 is formed on one lateral side of the reagent tube carrier 3, and a second clearance notch 304 is formed on the other lateral side of the reagent tube carrier 3, which is opposite to the first clearance notch 303. The first clearance notch 303 and the second clearance notch 304 are both through the length direction (i.e., longitudinal direction) of the reagent tube carrier 3 and are both connected to the accommodating space 301. The reagent tube carrier 3 has a first partition 305 between the first longitudinal end of the first clearance notch 303 and the first longitudinal end of the second clearance notch 304, and a second partition 306 between the second longitudinal end of the first clearance notch 303 and the second longitudinal end of the second clearance notch 304.

[0118] The first clamping member 801 is located at the position of the first clearance notch 303, and the second clamping member 802 is located at the position of the second clearance notch 304. One end of the first connecting member 803 is connected to the first clamping member 801, and the other end of the first connecting member 803 (such as a bolt) passes through the first partition 305 and is connected to the second clamping member 802. One end of the second connecting member 804 (such as a bolt) is connected to the first clamping member 801, and the other end of the second connecting member 804 passes through the second partition 306 and is connected to the second clamping member 802. The accommodating space 301 is between the first clamping member 801, the second clamping member 802, the first partition 305, and the second partition 306. Both the first elastic member and the second elastic member can be selected as springs. When the first elastic member and the second elastic member are compressed or return to their original state, the first clamping member 801, the second clamping member 802, the first connecting member 803, and the second connecting member 804 as a whole can move laterally relative to the reagent tube carrier 3.

[0119] When the reagent tube 4 is installed, the reagent tube 4 moves downward continuously and applies a pushing force to the second clamping member 802 in a direction away from the first clamping member 801. The first clamping member 801, the second clamping member 802, the first connecting member 803, and the second connecting member 804 move as a whole toward the direction of the second clamping member 802, and the first elastic member and the second elastic member are compressed. After the reagent tube 4 moves downward into place, under the elastic force of the first elastic member and the second elastic member, the second clamping member 802 continues to apply a pushing force to the reagent tube 4. The second clamping member 802 and the vertical sidewall of the accommodating space 301 on the side away from the second clamping member 802 work together to clamp the reagent tube 4. When it is necessary to remove the reagent tube 4, the operator applies a pushing force towards the second clamp 802 to the first clamp 801, further compressing the first elastic member and the second elastic member so that the first clamp 801, the second clamp 802, the first connector 803 and the second connector 804 move as a whole towards the direction of the second clamp 802, so as to release the clamping effect of the second clamp 802 and the vertical sidewall of the accommodating space 301 away from the second clamp 802 on the reagent tube 4. Then, an upward force is applied to the reagent tube 4 to remove the reagent tube 4.

[0120] Furthermore, a guide wall 307 for guiding the reagent tube 4 is formed on the side wall of the receiving space 301 near the second clamping member 802. When the operator installs the reagent tube 4, the reagent tube 4 can enter the receiving space 301 more easily under the guidance of the guide wall 307.

[0121] In one embodiment of this application, such as Figure 6 As shown, the ultrasonic mechanism 5 includes:

[0122] The probe mounting base 501 is set in the mounting area 2 and located on the longitudinal side of the extraction mechanism 6;

[0123] The ultrasonic probe 502 is used to output ultrasonic waves to the reagent in the reagent tube 4. The ultrasonic probe 502 is laterally movable on the probe mounting base 501 and faces the reagent tube carrier 3.

[0124] The drive connecting rod 503 passes through the probe mounting base 501 and forms a threaded connection with the probe mounting base 501.

[0125] The third driving component 504 is driven to connect with the driving connecting rod 503.

[0126] Specifically, the probe mounting base 501 includes a fifth connecting part 5013 and a third mounting part 5014 disposed at the bottom end of the fifth connecting part 5013. The ultrasonic probe 502 is mounted on the third mounting part 5014. The third driving member 504 can be a motor. The driving connecting rod 503 is connected to the driving end of the third driving member 504. The setting direction of the driving connecting rod 503 is consistent with the moving direction of the ultrasonic probe 502. The fifth connecting part 5013 is sleeved on the driving connecting rod 503 and forms a threaded connection with the driving connecting rod 503. When the motor rotates, it drives the driving connecting rod 503 to rotate together. The fifth connecting part 5013 moves along the axial direction of the driving connecting rod 503. When the fifth connecting part 5013 moves, it drives the third mounting part 5014 and the ultrasonic probe 502 connected to the third mounting part 5014 to move together, thereby causing the ultrasonic probe 502 to move closer to or further away from the reagent tube 4, so as to perform ultrasonic lysis of the reagent in the reagent tube 4.

[0127] If ultrasonic lysis of reagents in multiple reagent tubes 4 is required, the reagent tube carrier 3 is moved after the reagent in one reagent tube 4 has been ultrasonically lysed, so that the next reagent tube 4 containing the reagent to be ultrasonically lysed is aligned with the ultrasonic probe 502. This eliminates the need for multiple ultrasonic probes 502 to ultrasonically lyse the reagents in multiple reagent tubes 4 one by one, simplifying the structure of the ultrasonic mechanism 5, reducing its volume, and also helping to reduce the manufacturing cost of the nucleic acid extraction equipment. Furthermore, in this embodiment, the multiple reagent tubes 4 are integrally formed, that is, the multiple reagent tubes 4 are integrated into a reagent strip.

[0128] In one embodiment of this application, the ultrasonic probe 502 has a second arcuate wall 5021 formed on the side facing the reagent tube 4 for fitting against the outer peripheral wall of the reagent tube 4.

[0129] Specifically, since the ultrasonic probe 502 is attached to the reagent tube 4 through the second arc-shaped wall 5021, the contact area between the two is relatively large (compared to the point contact between the ultrasonic probe 502 and the reagent tube 4). Therefore, the ultrasonic probe 502 can directly transmit ultrasonic energy to the reagent tube 4 through the second arc-shaped wall 5021. The reagent tube 4 receives the ultrasonic energy directly and concentratedly at the contact point with the second arc-shaped wall 5021. That is, the ultrasonic energy is focused at the position in the reagent tube 4 corresponding to the second arc-shaped wall 5021 and transmitted to the reagent more efficiently, thereby increasing the intensity of the ultrasonic energy received by the reagent and thus improving the efficiency of cell ultrasonic lysis in the reagent.

[0130] In one embodiment of this application, the ultrasonic mechanism 5 further includes an elastic buffer component 505 disposed on the probe mounting base 501 for shock absorption of the ultrasonic probe 502. The elastic buffer component 505 can buffer the impact between the ultrasonic probe 502 and the reagent tube 4 when the third driving member 504 fails to drive or the ultrasonic probe 502 moves, thereby avoiding rigid impact between the ultrasonic probe 502 and the reagent tube 4, reducing the vibration of the ultrasonic probe 502 and / or the reagent tube 4, protecting the ultrasonic mechanism 5, and helping to extend the service life of the ultrasonic mechanism 5 and the pyrolysis device.

[0131] In one embodiment of this application, such as Figure 7 As shown, a second mounting space 5011 is formed on the probe mounting base 501, and a mounting groove 5012 is formed on the side wall of the second mounting space 5011 facing the reagent tube 4. The elastic buffer assembly 505 includes:

[0132] The first connecting rod 5051 is inserted into the probe mounting base 501 and passes through the second mounting space 5011;

[0133] The second connecting rod is horizontally spaced from the first connecting rod 5051. The second connecting rod is inserted into the probe mounting base 501 and passes through the second mounting space 5011.

[0134] The connecting plate 5052 is movably disposed on the first connecting rod 5051 and the second connecting rod and located in the second mounting space 5011. The end of the ultrasonic probe 502 away from the reagent tube 4 passes through the mounting groove 5012 and the connecting plate 5052 in sequence and extends in the direction away from the reagent tube 4.

[0135] The third elastic element 5053 is sleeved on the outside of the first connecting rod 5051. One end of the third elastic element 5053 abuts against the connecting plate 5052, and the other end of the third elastic element 5053 abuts against the side wall of the second installation space 5011 away from the reagent tube 4.

[0136] The fourth elastic element is sleeved on the outside of the second connecting rod. One end of the fourth elastic element abuts against the connecting plate 5052, and the other end of the fourth elastic element abuts against the side wall of the second mounting space 5011 away from the reagent tube 4.

[0137] Specifically, the second mounting space 5011 is formed on the third mounting part 5014. The first connecting rod 5051 and the second connecting rod are located on both sides of the ultrasonic probe 502. The first end of the first connecting rod 5051 is engaged with the side wall of the second mounting space 5011 near the reagent tube 4. The second end of the first connecting rod 5051 passes through the side wall of the second mounting space 5011 near the reagent tube 4, the connecting plate 5052, and the side wall of the second mounting space 5011 away from the reagent tube 4 in sequence. The first end of the second connecting rod is engaged with the second mounting space. On the side wall of the second mounting space 5011 near the reagent tube 4, the second end of the second connecting rod passes sequentially through the side wall of the second mounting space 5011 near the reagent tube 4, the connecting plate 5052, and the side wall of the second mounting space 5011 away from the reagent tube 4. The connecting plate 5052 is sleeved on the outside of the ultrasonic probe 502 and is tightly connected to the ultrasonic probe 502. The connecting plate 5052 and the ultrasonic probe 502 can move along the axial direction of the first connecting rod 5051 and the second connecting rod. The third elastic element 5053 and the fourth elastic element can both be selected as springs. If the ultrasonic probe 502 makes a movement error and impacts the reagent tube 4, the ultrasonic probe 502 can move away from the reagent tube 4 along with the connecting plate 5052. At this time, the third elastic element 5053 and the fourth elastic element are compressed and absorb the impact energy between the ultrasonic probe 502 and the reagent tube 4, preventing the impact energy from continuing to be transmitted and causing damage to other parts of the ultrasonic mechanism 5.

[0138] In one embodiment of this application, such as Figure 5 As shown, the reagent tube 4 includes a cylindrical portion 401 and an arcuate portion 402 located at the bottom end of the cylindrical portion 401. In the vertical direction, the position of the ultrasonic probe 502 corresponds to the position of the cylindrical portion 401 near the arcuate portion 402. When there is a small amount of reagent in the reagent tube 4, the above arrangement allows the ultrasonic energy transmitted by the ultrasonic probe 502 to act on the reagent as much as possible, instead of first transmitting it to the part of the reagent tube 4 that is not covered by the reagent, and then having the ultrasonic energy transmitted from the part of the reagent tube 4 to the part that is covered by the reagent before acting on the reagent (for example, if the liquid level of the reagent in the reagent tube 4 is low, and the position of the ultrasonic probe 502 is higher than the liquid level of the reagent, then after the ultrasonic energy is transmitted to the position of the reagent tube 4 corresponding to the ultrasonic probe 502, the reagent tube 4 still needs to transmit the ultrasonic energy downwards before it can act on the reagent). This helps to reduce the loss of ultrasonic energy.

[0139] In the description of this application, it should be understood that the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0140] In this application, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection, an electrical connection, or a connection that allows communication between components; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components, unless otherwise expressly limited. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0141] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "example," "specific example," or "some examples," etc., refer to specific features, structures, materials, or characteristics described in connection with that embodiment or example, which are included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples. Moreover, without contradiction, those skilled in the art can combine and integrate the different embodiments or examples described in this specification, as well as the features of different embodiments or examples.

[0142] Although embodiments of this application have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting this application. Those skilled in the art can make changes, modifications, substitutions and variations to the above embodiments within the scope of this application.

Claims

1. A nucleic acid extraction device, characterized in that, The nucleic acid extraction device includes: The container body has a moving area (1) and an installation area (2) that are distributed laterally adjacent to each other inside the container body; A reagent tube carrier (3) is provided in the movable area (1) and can move longitudinally. A receiving space (301) for accommodating reagent tubes (4) is formed on the reagent tube carrier (3). A probe inlet / outlet (302) is formed on the side wall of the receiving space (301) on the lateral side. An ultrasonic mechanism (5) is telescopically disposed in the mounting area (2), and the ultrasonic mechanism (5) is able to pass through the probe inlet / outlet (302) and extend into the receiving space (301) in an extended state; The extraction mechanism (6) is disposed in the installation area (2) and located on the longitudinal side of the ultrasonic mechanism (5).

2. The nucleic acid extraction device according to claim 1, characterized in that, The extraction mechanism (6) includes: A first driving element (601) is disposed in the mounting area (2) and distributed longitudinally; The magnetic rod sleeve assembly (602) is vertically movable in the moving area (1) and is drivenly connected to the first driving member (601); The second driving member (603) is disposed above the first driving member (601) and distributed longitudinally. In the transverse direction, the second driving member (603) overlaps with the first driving member (601). A magnetic rod (604) is vertically movable in the moving area (1). The magnetic rod (604) is driven to connect with the second driving member (603) and can partially extend into the interior of the magnetic rod sleeve assembly (602) or separate from the magnetic rod sleeve assembly (602) under the driving action of the second driving member (603).

3. The nucleic acid extraction device according to claim 2, characterized in that, The extraction mechanism (6) also includes: A vertical plate (605) is vertically arranged in the installation area (2), the first driving member (601) is arranged at the bottom end of the vertical plate (605), and the second driving member (603) is arranged at the top end of the vertical plate (605); The first synchronous belt assembly (606) is vertically disposed on one side of the upright plate (605) and drivenly connected to the first driving member (601). The first synchronous belt (6061) of the first synchronous belt assembly (606) is connected to the magnetic rod sleeve assembly (602). The second synchronous belt assembly (607) is vertically disposed on the side of the upright plate (605) away from the first synchronous belt assembly (606) and is drivenly connected to the second driving member (603). The second synchronous belt (6071) of the second synchronous belt assembly (607) is connected to the magnetic rod (604).

4. The nucleic acid extraction device according to claim 3, characterized in that, The upright plate (605) has a first mounting space (6051) for mounting the tensioning mechanism, and the first mounting space (6051) is located between the first driving member (601) and the second driving member (603).

5. The nucleic acid extraction device according to claim 1, characterized in that, The nucleic acid extraction device also includes a heating element (7) disposed at the bottom of the containing space (301) for heating the reagent in the reagent tube (4).

6. The nucleic acid extraction device according to claim 5, characterized in that, The heating element (7) has a recessed groove (701) that is recessed from top to bottom and is used to accommodate part of the reagent tube (4).

7. The nucleic acid extraction device according to claim 6, characterized in that, The heating element (7) includes a base portion (702) and a stop portion (703). The receiving groove (701) is formed on the base portion (702), and the stop portion (703) is disposed on the top of the base portion (702) and can apply a lateral stopping force to the reagent tube (4).

8. The nucleic acid extraction device according to claim 1, characterized in that, The nucleic acid extraction device also includes a clamping mechanism (8) disposed on the reagent tube carrier (3) and used to clamp the reagent tube (4).

9. The nucleic acid extraction device according to claim 1, characterized in that, The ultrasonic mechanism (5) includes: A probe mounting base (501) is disposed in the mounting area (2) and located on one longitudinal side of the extraction mechanism (6); An ultrasonic probe (502) is used to output ultrasonic waves to the reagent in the reagent tube (4). The ultrasonic probe (502) is laterally movable and is mounted on the probe mounting base (501) and faces the reagent tube carrier (3). The drive connecting rod (503) passes through the probe mounting base (501) and forms a threaded connection with the probe mounting base (501); The third driving component (504) is drivingly connected to the driving connecting rod (503).

10. The nucleic acid extraction device according to claim 9, characterized in that, The ultrasonic mechanism (5) further includes an elastic buffer assembly (505) disposed on the probe mounting base (501) and used to dampen the ultrasonic probe (502).

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