Coil pipe type fermentation device and continuous solid state fermentation system

Abstract

The application discloses a coil pipe type fermentation device and a continuous solid-state fermentation system, relates to the technical field of solid-state fermentation, and can realize disassembly, offline high-temperature and high-pressure sterilization, and complete sealing of the whole device, so that bacterial contamination is completely avoided from sterilization and fermentation environment of the device, pure culture of strains is realized, and the quality and yield of fermentation products are improved. Meanwhile, the sterilization mode is flexible, supports two modes of assembly after offline sterilization and online sterilization after assembly, is suitable for different use scenes, can effectively avoid bacterial contamination, and realizes pure culture of strains. The coil pipe and the flexible stirring assembly are matched, the device volume is greatly reduced, the space utilization is improved, and the whole link of test, pilot test and production is adapted. Through multi-stage coil pipe series connection design and independent temperature control and parameter regulation of each coil pipe, fermentation conditions can be accurately controlled according to different fermentation stages. Finally, continuous, automatic and accurate operation of solid-state fermentation is realized, and fermentation production efficiency and product stability are improved.

Description

Technical Field

[0001] This invention relates to the field of solid-state fermentation technology, and more particularly to a coil-type fermentation device and a continuous solid-state fermentation system. Background Technology

[0002] Solid-state fermentation is a microbial fermentation process that uses a solid substrate as a nutrient carrier and takes place under conditions with little or no free water. With its advantages such as high raw material utilization, relatively low equipment investment, and easy product separation, it occupies a pivotal position in bioengineering fields such as feed fermentation, food brewing, and the preparation of biopharmaceutical active ingredients. As bioengineering technology continues to develop, the application scenarios of solid-state fermentation are constantly expanding, and various industries are increasingly demanding higher production efficiency, controllability of the fermentation process, purity of microbial cultures, and adaptability of equipment.

[0003] However, existing solid-state fermentation equipment and processes still have many technical shortcomings, making it difficult to meet the needs of modern fermentation production: First, existing devices are mostly monolithic structures, making it impossible to disassemble and sterilize components offline. Some dead-end areas are difficult to sterilize completely, resulting in poor sterilization effects and easy contamination by other microorganisms. This prevents the pure cultivation of the microbial strain, thus affecting the quality and yield of the fermentation product. Second, the production mode is intermittent, lacking a continuous microbial cultivation and material fermentation system. The microbial cultivation and fermentation process are disconnected, requiring multiple manual inoculations and feedings, leading to low production efficiency and susceptibility to contamination from manual operation. Third, the space utilization of the equipment... Low utilization rate: Traditional fermenters are mostly vertical or horizontal chamber structures, which are bulky and only suitable for large-scale industrial production. They are difficult to adapt to the small-batch fermentation needs of laboratory experiments and pilot-scale stages, and the equipment has poor versatility between the experimental and production stages. Finally, the mixing effect is poor. The stirring shaft of existing fermentation equipment is mostly a rigid structure, which cannot be adapted to irregularly shaped fermentation chambers. There are gaps between the stirring blades and the chamber wall, and the material is easy to stick to the chamber wall and stirring shaft. Moreover, it can only achieve single rotation stirring, and the material propulsion speed and mixing intensity adjustment methods are limited. The mass transfer and heat transfer efficiency is low, resulting in uneven mixing of fermentation substrate and inoculum, and insufficient fermentation process.

[0004] In conclusion, there is an urgent need for a coil-type fermentation device and a continuous solid-state fermentation system to solve the above problems. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a coil-type fermentation device and a continuous solid-state fermentation system to solve the problems mentioned in the background section.

[0006] This invention proposes a coil-type fermentation device, including a temperature control tank, a coil, a stirring head, and a flexible stirring assembly. The coil is disposed inside the temperature control tank, and its two ends are respectively inserted into the top and bottom side walls of the temperature control tank. The flexible stirring assembly is disposed inside the coil. The stirring head is installed at the end of the flexible stirring assembly away from the temperature control tank.

[0007] The flexible stirring assembly includes a flexible stirring shaft, a sliding screw, and a magnet; the flexible stirring shaft extends from the inside of the coil to the outside, and the sliding screw is connected to the end of the flexible stirring shaft away from the temperature control tank, and the sliding screw can slide along the axial direction of the flexible stirring shaft; the magnet is fixedly installed on the outer wall of the end of the sliding screw away from the flexible stirring shaft.

[0008] The stirring head includes a connecting shaft, a speed-regulating motor, and an electromagnetic coil. The speed-regulating motor is located at the end of the stirring head furthest from the flexible stirring assembly. The output end of the speed-regulating motor is fixedly connected to the connecting shaft, which is inserted into the end of the sliding screw furthest from the flexible stirring shaft. The other end of the sliding screw is connected to the flexible stirring shaft, thereby enabling the speed-regulating motor to control the rotation of the flexible stirring assembly inside the coil. An electromagnetic coil is located on the outer wall of the connecting shaft near the magnet. The charging and discharging of the electromagnetic coil allows the magnet to move closer to or away from the coil. The sliding screw slides along the axial direction of the flexible stirring shaft, causing it to vibrate axially. The stirring head can achieve both radial rotation and axial vibration of the flexible stirring assembly.

[0009] In a preferred embodiment of the present invention, flexible stirring blades are fixedly installed on the outer wall of the flexible stirring shaft.

[0010] In a preferred embodiment of the present invention, two feeding ports are fixedly installed on one side of the top of the coil, and the feeding ports are located at one end of the flexible stirring shaft.

[0011] In a preferred embodiment of the present invention, a discharge port is installed at the lower end of the coil, and a vent is provided at one end of the discharge port.

[0012] In a preferred embodiment of the present invention, a thermometer is fixedly installed on the outer wall of the temperature control tank.

[0013] In a preferred embodiment of the present invention, the flexible stirring assembly further includes a vent valve, which is fixedly installed at one end of the flexible stirring shaft.

[0014] In a preferred embodiment of the present invention, the flexible stirring shaft is fitted to the coil body, and the flexible stirring blades are bent and closely attached to the inner wall of the coil.

[0015] In a preferred embodiment of the present invention, the flexible stirring shaft is made of materials including metal, rubber, and plastic, and can bend with the coil when rotating.

[0016] In a preferred embodiment of the present invention, the flexible stirring blade is made of metal, rubber, or plastic.

[0017] In a preferred embodiment of the present invention, a flange is installed at the end of the coil away from the temperature control tank for easy disassembly and assembly.

[0018] The present invention also provides a continuous solid-state fermentation system, including a seed silo, a seed tank, a fermentation silo, and a coil-type fermentation device, wherein a plurality of coil-type fermentation devices are provided, such as the coil-type fermentation device described above, and the plurality of coil-type fermentation devices are connected in sequence.

[0019] The seed hopper is located upstream of the seed tank, which is located upstream of the flexible stirring assembly. Corresponding to the seed tank, a fermentation hopper is also located upstream of the flexible stirring assembly. The seed tank and the fermentation hopper are respectively connected to the two feeding ports.

[0020] In a preferred embodiment of the present invention, the seed tank includes a speed-regulating motor, a feed inlet, a guide cylinder, a stirring screw, and a jacket; the speed-regulating motor is fixedly installed on the top of the seed tank, and the output end of the speed-regulating motor is fixedly connected to the stirring screw, which extends from the top of the seed tank to the interior of the seed tank, and the guide cylinder is fitted around the outer side of the bottom of the stirring screw; the top of the seed tank is provided with a feed inlet, and the bottom of the outer wall of the seed tank is fitted with a jacket.

[0021] In a preferred embodiment of the present invention, the top of the seed tank is also provided with a sterile gas inlet.

[0022] In a preferred embodiment of the present invention, the seed container is further equipped with a thermometer, which is inserted into the inside of the seed container.

[0023] In a preferred embodiment of the present invention, a first feeding screw is provided at the bottom of the seed tank, a second speed-regulating motor is provided at one end of the first feeding screw, and the connection between the seed tank and the flexible stirring assembly is provided at the end of the first feeding screw away from the second speed-regulating motor.

[0024] In a preferred embodiment of the present invention, two adjacent coil-type fermentation devices are connected, and the upstream discharge port is connected to the downstream feed port of the flexible stirring assembly.

[0025] In a preferred embodiment of the present invention, a windbreak is installed between the upstream discharge port and the downstream feed port of the flexible stirring assembly.

[0026] In a preferred embodiment of the present invention, an electric valve is installed at the end of the coiled fermentation device that is away from the rest of the coiled fermentation devices.

[0027] In a preferred embodiment of the present invention, a conveying assembly is provided between the seed silo and the seed tank.

[0028] In a preferred embodiment of the present invention, a conveying component is provided between the fermentation silo and the flexible stirring component.

[0029] In a preferred embodiment of the present invention, the conveying assembly includes a speed-regulating motor four and a feeding screw two. The speed-regulating motor four is disposed at one end of the feeding screw two, and the end of the feeding screw two away from the speed-regulating motor four is connected to the downstream.

[0030] In a preferred embodiment of the present invention, the continuous solid-state fermentation system is provided with two modes: offline sterilization followed by assembly and online sterilization followed by assembly. When the continuous solid-state fermentation system is in the online sterilization followed by assembly mode, gas is introduced and pressure is maintained after online sterilization.

[0031] Compared with the prior art, the technical solution of this application has the following beneficial effects:

[0032] 1. The coil-type fermentation device and continuous solid-state fermentation system presented in this invention can achieve offline high-temperature and high-pressure sterilization by setting each unit to be disassembled, thereby achieving pure culture of microorganisms with a low rate of contamination. The entire device is a fully sealed structure, which can be circulated with sterile gas to achieve micro-positive pressure operation. It completely avoids contamination of microorganisms from both the sterilization of the device and the fermentation environment, solving the problem of poor sterilization effect of existing devices, realizing pure culture of microorganisms, and improving the quality and yield of fermentation products. At the same time, the sterilization method is flexible, supporting two modes: offline sterilization followed by assembly and online sterilization after assembly, which can adapt to different usage scenarios and effectively avoid contamination of microorganisms, achieving pure culture of microorganisms.

[0033] 2. The coil-type fermentation device and continuous solid-state fermentation system presented in this invention can achieve continuous fermentation by setting up a seed tank, completing the continuous feeding of culture medium, continuous cultivation of inoculum and stable output. Combined with the continuous feeding of the silo unit and the continuous fermentation of the coil fermentation body, a full-process continuous operation system is constructed, which is "from seed cultivation, through material mixing, continuous fermentation, to quantitative output". This replaces the traditional intermittent feeding and inoculation mode, reduces manual operation links, not only improves production efficiency, but also avoids the pollution risk introduced by manual operation.

[0034] 3. The coil-type fermentation device and continuous solid-state fermentation system presented in this invention, by setting up multi-stage series coils, with each fermentation stage independently equipped with a temperature control tank, can precisely control the temperature of different fermentation stages. At the same time, each fermentation stage is equipped with independent gas inlet and outlet, inoculum, and material replenishment port, which can adjust the gas composition, dissolved oxygen, material moisture, and pH of each fermentation stage. This achieves independent and precise control of key parameters at different stages of fermentation, solves the defects of single-parameter control in existing devices, meets the differentiated fermentation conditions required at different stages of solid-state fermentation, and improves the metabolic efficiency of inoculum and the fermentation effect.

[0035] 4. The coil-type fermentation device and continuous solid-state fermentation system presented in this invention significantly reduce the footprint of the device and improve space utilization by replacing the traditional vertical or horizontal cavity structure with a coil-type fermentation main body. At the same time, the diameter, length and number of series stages of the coil can be flexibly designed according to fermentation needs, and the volume of the seed tank and the material silo can also be selected as needed. It can be adapted to different scenarios such as small-batch laboratory experiments, pilot-scale amplification and large-scale industrial production, realizing the device's versatility in both experimental and production stages and reducing the equipment investment costs in research and development and production.

[0036] 5. The coil-type fermentation device and continuous solid-state fermentation system presented in this invention, by setting up a flexible stirring unit, allows the flexible stirring shaft to bend freely along the coil trajectory, and the flexible stirring blades to fit tightly against the inner wall of the coil in a natural state, eliminating dead zones in the stirring. At the same time, the stirring unit has dual functions of rotational stirring and axial vibration. Rotational stirring achieves material mixing and propulsion, while axial vibration achieves scraping and anti-sticking. Moreover, the stirring intensity and material propulsion speed can be flexibly adjusted through various parameters to adapt to fermentation substrates with different viscosities and particle sizes, significantly improving the uniformity of mixing between materials and inoculum, improving the mass and heat transfer conditions during the fermentation process, and avoiding the problem of incomplete fermentation caused by material sticking. Attached Figure Description

[0037] Figure 1 This is a schematic diagram of the structure of a coil-type fermentation device and a continuous solid-state fermentation system according to the present invention;

[0038] Figure 2 This is a schematic diagram of the structure of the seed tank in a coil-type fermentation device and a continuous solid-state fermentation system according to the present invention.

[0039] Figure 3 This is a schematic diagram of the structure of the stirring head and flexible stirring assembly in the coil-type fermentation device and continuous solid-state fermentation system of the present invention.

[0040] In the diagram: 1. Seed silo; 2. Seed tank; 21. Speed-regulating motor 1; 22. Feed inlet; 23. Guide cylinder; 24. Stirring screw; 25. Jacket; 26. Thermometer 1; 27. Feed screw 1; 28. Speed-regulating motor 2; 29. ​​Sterile gas inlet; 3. Fermentation silo; 4. Stirring head; 41. Connecting shaft; 42. Speed-regulating motor 3; 43. Electromagnetic coil; 5. Windproof device; 6. Vent; 7. Thermometer 2; 8. Electric valve; 9. Coil; 10. Flexible stirring assembly; 101. Feed port; 102. Vent valve; 103. Flexible stirring shaft; 104. Flexible stirring blade; 105. Sliding screw; 106. Magnet; 11. Temperature control tank; 12. Transmission assembly; 121. Speed-regulating motor 4; 122. Feed screw 2; 13. Discharge port; 14. Flange. Detailed Implementation

[0041] First, it should be noted that in different described embodiments, the same components are given the same reference numerals or the same component names. The disclosure contained throughout this specification can be applied semantically to the same components having the same reference numerals or the same component names. The location descriptions selected in the specification, such as upper, lower, lateral, etc., also refer to the directly described and illustrated figures and are semantically applied to the new location when the location changes.

[0042] Example 1:

[0043] Please refer to the following: A coil-type fermentation device and a continuous solid-state fermentation system. Figures 1-3 The coil 9 is disposed inside the temperature control tank 11, and the two ends of the coil 9 are respectively inserted into the top side wall and the bottom side wall of the temperature control tank 11; the flexible stirring assembly 10 is disposed inside the coil 9; the stirring head 4 is installed at the end of the flexible stirring assembly 10 away from the temperature control tank 11.

[0044] like Figure 3 As shown, the flexible stirring assembly 10 includes a flexible stirring shaft 103, a sliding screw 105, a vent valve 102, and a magnet 106. The flexible stirring shaft 103 extends from the inside of the temperature control tank 11 to the outside of the temperature control tank 11. The sliding screw 105 is connected to one end of the flexible stirring shaft 103 away from the temperature control tank 11, and the sliding screw 105 can slide along the axial direction of the flexible stirring shaft 103. The magnet 106 is fixedly installed on the outer wall of the end of the sliding screw 105 away from the flexible stirring shaft 103. A flexible stirring blade 104 is fixedly installed on the outer wall of the flexible stirring shaft 103. The vent valve 102 is fixedly installed on one side of the flexible stirring shaft 103.

[0045] like Figure 3As shown, the stirring head 4 includes a connecting shaft 41, a speed-regulating motor 42, and an electromagnetic coil 43. The speed-regulating motor 42 is located at the end of the stirring head 4 away from the flexible stirring assembly 10. The output end of the speed-regulating motor 42 is fixedly connected to the connecting shaft 41. The connecting shaft 41 is inserted into the end of the sliding screw 105 away from the flexible stirring shaft 103. An electromagnetic coil 43 is provided on the outer wall of the connecting shaft 41 near the magnet 106. The magnet 106 can move away from or closer to the electromagnetic coil 43 by charging and discharging the electromagnetic coil 43. The sliding screw 105 slides along the flexible stirring shaft 103, controlling the connection position between the connecting shaft 41 and the flexible stirring assembly 10, thereby realizing the movement of the coil 9 inside the temperature control tank 11 controlled by the speed-regulating motor 42.

[0046] The working principle of the flexible mixing unit is as follows: the speed-regulating motor 342 drives the flexible mixing shaft 103 to rotate, and the flexible mixing blades 104 rotate with the flexible mixing shaft 103 to mix the materials. At the same time, the deformation angle of the flexible mixing blades 104 changes with the rotation speed, pushing the materials along the main body of the coil fermentation towards the discharge end. Meanwhile, the sliding screw 105 drives the flexible mixing shaft to perform axial reciprocating vibration at a frequency of 10-50Hz. The flexible mixing blades 104 scrape and rebound with the vibration, further improving the mixing effect of the materials and preventing material adhesion. By adjusting the speed of the speed-regulating motor 342, the frequency of axial vibration, and by replacing the flexible mixing blades 104 with different spacing, length, hardness, and bending direction, the mixing intensity and material propulsion speed can be flexibly controlled to adapt to the needs of different fermentation substrates and fermentation stages, providing good mass and heat transfer conditions for materials and inoculum.

[0047] like Figure 3 As shown, two feeding ports 101 are fixedly installed on one side of the top of the coil 9, and the feeding ports 101 are located at one end of the flexible stirring shaft 103; a flange 14 is installed at the end of the coil 9 away from the temperature control tank 11, and the two ends can be disassembled and sterilized separately using the flange 14, which is convenient for disassembly and assembly.

[0048] like Figure 1 As shown, a discharge port 13 is installed at the bottom of the temperature control tank 11, and a vent 6 is provided at one end of the discharge port 13; a thermometer 7 is fixedly installed on the outer wall of the temperature control tank 11.

[0049] In use, the flexible stirring shaft 103 fits into the main body of the coil 9, and the flexible stirring blade 104 is bent and closely attached to the inner wall of the coil 9.

[0050] Furthermore, the flexible stirring shaft 103 is made of materials including metal, rubber, and plastic, and can bend with the coil 9 when rotating; the flexible stirring blade 104 is made of metal, rubber, and plastic.

[0051] Example 2:

[0052] Please refer to a continuous solid-state fermentation system. Figures 1-3 It includes a seed silo 1, a seed tank 2, a fermentation silo 3, and a coil-type fermentation device. Several coil-type fermentation devices are provided, such as the coil-type fermentation device described above, and several coil-type fermentation devices are connected in sequence.

[0053] like Figure 1 As shown, the seed hopper 1 is located upstream of the seed tank 2, the seed tank 2 is located upstream of the flexible stirring assembly 10, and a fermentation hopper 3 is also located upstream of the flexible stirring assembly 10, corresponding to the seed tank 2; the seed tank 2 and the fermentation hopper 3 are respectively connected to the two feeding ports 101.

[0054] like Figure 2 As shown, the seed tank 2 includes a speed-regulating motor 21, a feed inlet 22, a guide cylinder 23, a stirring screw 24, and a jacket 25. The speed-regulating motor 21 is fixedly installed on the top of the seed tank 2, and the output end of the speed-regulating motor 21 is fixedly connected to the stirring screw 24. The stirring screw 24 extends from the top of the seed tank 2 to the interior of the seed tank 2, and the guide cylinder 23 is fitted onto the outer side of the bottom of the stirring screw 24. The feed inlet 22 is provided on the top of the seed tank 2, and the jacket 25 is fitted onto the bottom of the outer wall of the seed tank 2. A sterile gas inlet 29 is also provided on the top of the seed tank 2. A thermometer 26 is also installed in the seed tank 2 and is inserted into the interior of the seed tank 2.

[0055] like Figure 2 As shown, a feed screw 27 is provided at the bottom of the seed tank 2, and a speed-regulating motor 28 is provided at one end of the feed screw 27. The connection between the seed tank 2 and the flexible stirring assembly 10 is provided at the end of the feed screw 27 away from the speed-regulating motor 28.

[0056] like Figure 1 As shown, two adjacent coil fermentation devices are connected, and a windbreak 5 is installed between the upstream discharge port 13 and the downstream feed port 101 of the flexible stirring assembly 10; an electric valve 8 is installed at the end of the coil fermentation device away from the other coil fermentation devices.

[0057] like Figure 1As shown, a transmission assembly 12 is provided between the seed silo 1 and the seed tank 2; a transmission assembly 12 is provided between the fermentation silo 3 and the flexible stirring assembly 10; the transmission assembly 12 includes a speed-regulating motor 121 and a feeding screw 122, the speed-regulating motor 121 is located at one end of the feeding screw 122, and the end of the feeding screw 122 away from the speed-regulating motor 121 is connected to the downstream.

[0058] Furthermore, the continuous solid-state fermentation system is equipped with two modes: offline sterilization followed by assembly and online sterilization followed by assembly.

[0059] When the continuous solid-state fermentation system is in the online sterilization mode after assembly, gas is introduced to maintain pressure after online sterilization.

[0060] When the continuous solid-state fermentation system is in offline sterilization and assembly mode, the seed silo 1 is filled with material, sterilized offline, and connected to the seed tank 2. Simultaneously, the fermentation silo 3 is filled with material, sterilized, and connected to the stirring head 4. A certain amount of solid material is added to the seed tank 2 for inoculation, stirring, and temperature control. After the seeds reach a certain stage of growth, the feed screw 27 is controlled to allow the material to enter the main body of the fermentation coil 9 at a certain speed. Simultaneously, the feed screw 122 of the fermentation silo 3 is controlled to allow the material and seeds to enter the main body of the fermentation coil 9 at a certain speed. By adjusting the flexible stirring speed and axial vibration frequency, and selecting the spacing, length, hardness, and bending direction of the flexible stirring blades 104, the stirring intensity and material propulsion speed are controlled.

[0061] The main body of the fermentation coil 9 is located within the temperature control tank 11. It is equipped with inlet / outlet ports for microorganisms and materials, sampling ports, gas inlets / outlets, and temperature, pH, gas composition, and material composition detection devices at appropriate locations. The fermentation coil 9 can be connected in multiple stages, allowing independent temperature control and the introduction of different gases at different stages. It can also be supplemented with appropriate materials or microorganisms, and water can be added to adjust moisture content or pH value can be adjusted using acid / alkali. This enables independent and precise control of fermentation parameters at different fermentation stages. After fermentation is completed under specific conditions, the material is discharged via an electric valve 8 or a discharge device. The entire system is closed, preventing material contamination from the external environment.

[0062] A certain amount of solid material is added to the continuous seed tank 2, sterilized off-site, and then inoculated after assembly. Alternatively, inoculation can be performed in a sterile room followed by assembly. The temperature and stirring speed are controlled to cultivate the inoculum. Once the inoculum reaches a certain standard, the feed screw 122 above the seed hopper 1 is opened, allowing material to be fed into the seed tank 2 at a certain speed. After further cultivation for a certain period, the feed screw 27 is adjusted to allow material to enter the main body of the coil 9 through the stirring head 4 at a certain speed. By adjusting the temperature, stirring speed, hopper feeding speed, and seed output speed of the seed tank 2, the seed state is controlled, providing a stable inoculum for fermentation.

[0063] The fermentation materials and inoculum enter the stirring head 4 separately, and together they enter the main body of the fermentation coil 9 under the push of the sliding screw 105. The sliding screw 105 is equipped with a magnet 106, which can vibrate axially under external electromagnetic action, thereby driving the stirring vibration.

[0064] The flexible mixing assembly 10 consists of a flexible mixing shaft 103 and flexible mixing blades 104. The flexible mixing shaft 103 is fitted into the main body of the coil 9, and the flexible mixing blades 104 are bent and closely attached to the inner wall of the coil 9. When rotating, the flexible mixing blades 104 mix the materials, and the changing angle propels the materials. By adjusting the mixing speed, axial vibration frequency, spacing, length, hardness, and bending direction of the flexible mixing blades 104, the mixing intensity and material propulsion speed can be adjusted. This provides better mass transfer and heat transfer conditions for various materials.

[0065] The flexible stirring shaft 103 is fitted into the main body of the coil 9, and the flexible stirring blades 104 are bent and tightly attached to the inner wall of the coil 9. The flexible stirring shaft 103 is made of materials including metal, rubber, and plastic, and can be flexible and continuous or segmented, and can bend with the coil 9 when rotating. The flexible stirring blades 104 are made of metal, rubber, or plastic, and are tightly attached to the inner wall of the coil 9. When the flexible stirring shaft 103 rotates, it supports the flexible stirring shaft 103 to be located at the center of the coil 9, and at the same time, it bends to a certain extent under the resistance of the material, and rebounds when the flexible stirring shaft 103 vibrates.

[0066] The pusher screw of the stirring head 4 adopts a sleeve shaft structure and is equipped with a magnet 106. Under the action of the external electromagnetic coil 43, it can slide axially, drive flexible stirring vibration, prevent material sticking, and produce a better stirring effect.

[0067] Each unit can be disassembled and sterilized offline at high temperature and pressure, achieving pure culture of microorganisms with low contamination rate. The entire device is a fully sealed structure, allowing the introduction of sterile gas to achieve micro-positive pressure operation, completely avoiding contamination from both the sterilization of the device and the fermentation environment. This solves the problem of poor sterilization effect of existing devices, achieving pure culture of microorganisms and improving the quality and yield of fermentation products. At the same time, the sterilization method is flexible, supporting both offline sterilization followed by assembly and online sterilization after assembly, adapting to different usage scenarios and effectively avoiding contamination of microorganisms to achieve pure culture of microorganisms. By configuring a continuous seed culture tank, continuous culture and stable output of microorganisms are achieved. Continuous fermentation can be achieved, completing continuous feeding of culture medium, continuous culture and stable output of microorganisms. Combined with the continuous feeding of the silo unit and the continuous fermentation of the main fermentation unit in coil 9, a full-process continuous operation system is constructed, "from seed culture, through material mixing, continuous fermentation, to quantitative output", replacing the traditional intermittent feeding and inoculation mode, reducing manual operation links, improving production efficiency, and avoiding the contamination risk introduced by manual operation.

[0068] The combination of coil 9 and flexible stirring assembly 10 significantly reduces the size of the device, improves space utilization, and is suitable for all stages of testing, pilot production, and manufacturing. Through the multi-stage series design of coil 9 and the independent temperature control and parameter adjustment of each coil 9 stage, each fermentation stage is independently equipped with a temperature control tank 11, which can accurately control the temperature of different fermentation stages. At the same time, each fermentation stage is equipped with independent gas inlet and outlet and inoculum / material replenishment port, which can adjust the gas composition, dissolved oxygen, material moisture and pH of each fermentation stage. This enables independent and precise control of key parameters at different stages of fermentation, solves the defects of single parameter control in existing devices, meets the differentiated fermentation conditions required at different stages of solid-state fermentation, and improves the metabolic efficiency of inoculum and fermentation effect.

[0069] The coil-type fermentation unit replaces the traditional vertical or horizontal cavity structure, significantly reducing the footprint of the device and improving space utilization. At the same time, the diameter, length, and number of series stages of the coil 9 can be flexibly designed according to fermentation needs, and the volume of the seed tank 2 and the material silo can also be selected as needed. It can be adapted to different scenarios such as small-batch laboratory experiments, pilot-scale amplification, and large-scale industrial production, realizing the device's versatility in both experimental and production stages and reducing the equipment investment costs in research and development and production.

[0070] The flexible stirring shaft 103 can bend freely along the trajectory of the coil 9, and the flexible stirring blades 104 are in close contact with the inner wall of the coil 9 in their natural state, eliminating any dead corners in the stirring. At the same time, the stirring unit has dual functions of rotational stirring and axial vibration. Rotational stirring achieves material mixing and propulsion, while axial vibration achieves scraping and anti-sticking. Moreover, the stirring intensity and material propulsion speed can be flexibly adjusted through various parameters to adapt to fermentation substrates with different viscosities and particle sizes, greatly improving the uniformity of mixing between materials and inoculum, improving the mass and heat transfer conditions during the fermentation process, and avoiding the problem of incomplete fermentation caused by material sticking.

[0071] By utilizing the multi-parameter adjustment and axial vibration design of the flexible stirring blade 104, the problem of material adhesion is solved, the mixing and mass and heat transfer effects of materials are improved, and the continuous, automated and precise operation of solid-state fermentation is finally realized, thereby improving fermentation production efficiency and product stability.

[0072] The continuous fermentation seed tank 2 can precisely control the culture process of the strain through precise temperature control by the jacket 25, speed regulation mixing by the stirring screw 24, and continuous feeding of the culture medium, ensuring that the strain is always in the best growth state. At the same time, the seed tank 2 can achieve continuous and stable output of the strain, providing a uniform source of strain for the fermentation body of the coil 9, avoiding the problem of unstable fermentation effect caused by the fluctuation of the strain state in the traditional fermentation process, and ensuring the consistency of fermentation product yield and quality.

[0073] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A coil-type fermentation device, characterized in that, The device includes a temperature control tank (11), a coil (9) is disposed inside the temperature control tank (11), and the two ends of the coil (9) are respectively inserted into the top side wall and the bottom side wall of the temperature control tank (11); the flexible stirring assembly (10) is disposed inside the coil (9); and the stirring head (4) is installed at the end of the flexible stirring assembly (10) away from the temperature control tank (11).

2. The coil-type fermentation device according to claim 1, characterized in that: The flexible stirring assembly (10) includes a flexible stirring shaft (103), a sliding screw (105), and a magnet (106); the flexible stirring shaft (103) extends from the inside of the control tube (9) to the outside, and the sliding screw (105) is connected to one end of the flexible stirring shaft (103) away from the temperature control tank (11), and the sliding screw (105) can slide along the axial direction of the flexible stirring shaft (103); the magnet (106) is fixedly installed on the outer wall of the end of the sliding screw (105) away from the flexible stirring shaft (103).

3. The coil-type fermentation device according to claim 2, characterized in that: The stirring head (4) includes a connecting shaft (41), a speed-regulating motor (42), and an electromagnetic coil (43). The speed-regulating motor (42) is located at one end of the stirring head (4) away from the flexible stirring assembly (10). The output end of the speed-regulating motor (42) is fixedly connected to the connecting shaft (41). The connecting shaft (41) is inserted into one end of the sliding screw (105) away from the flexible stirring shaft (103). An electromagnetic coil (43) is provided on the outer wall of the connecting shaft (41) near the magnet (106).

4. The coil-type fermentation device according to claim 2, characterized in that: Flexible stirring blades (104) are fixedly installed on the outer wall of the flexible stirring shaft (103).

5. The coil-type fermentation device according to claim 4, characterized in that: Two feeding ports (101) are fixedly installed on one side of the top of the coil (9), and the feeding ports (101) are located at one end of the flexible stirring shaft (103).

6. The coil-type fermentation apparatus according to claim 5, characterized in that: The lower end of the coil (9) is equipped with a discharge port (13), and one end of the discharge port (13) is provided with a vent (6).

7. A continuous solid-state fermentation system, characterized in that: It includes a seed silo (1), a seed tank (2), a fermentation silo (3), and a coil-type fermentation device. Several coil-type fermentation devices are provided, as described in claim 6, and several coil-type fermentation devices are connected in sequence.

8. The continuous solid-state fermentation system according to claim 7, characterized in that: The seed hopper (1) is located upstream of the seed tank (2), which is located upstream of the flexible stirring assembly (10). Corresponding to the seed tank (2), a fermentation hopper (3) is also located upstream of the flexible stirring assembly (10). The seed tank (2) and the fermentation hopper (3) are respectively connected to the two feeding ports (101).

9. The continuous solid-state fermentation system according to claim 8, characterized in that: Two adjacent coil fermentation devices are connected, with the upstream discharge port (13) connected to the downstream feed port (101) of the flexible stirring assembly (10); a windbreak (5) is installed between the upstream discharge port (13) and the downstream feed port (101) of the flexible stirring assembly (10); an electric valve (8) is installed at the end of the coil fermentation device away from the other coil fermentation devices.

10. The continuous solid-state fermentation system according to claim 9, characterized in that: The continuous solid-state fermentation system has two modes: offline sterilization followed by assembly and online sterilization after assembly. When the continuous solid-state fermentation system is in the online sterilization after assembly mode, gas is introduced and pressure is maintained after online sterilization.

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