Trichoderma harzianum jt3 and its use

Abstract

This invention discloses a Trichoderma harzianum JT3 and its applications, relating to the field of microbial technology. The aforementioned Trichoderma harzianum ( Trichoderma harzianum JT3, deposited on September 22, 2025, at the China Center for Type Culture Collection (CCTCC), accession number: CCTCC NO: M 20252082, address: Wuhan University, Wuhan, China. This invention also provides applications of *Trichoderma harzianum* JT3. The *Trichoderma harzianum* JT3 strain provided by this invention can produce various lignocellulose-degrading enzymes, efficiently degrading lignin, cellulose, and hemicellulose in agricultural waste such as straw and producing ethyl styrosinate. Simultaneously, it exhibits significant inhibitory effects on various plant pathogens, effectively solving the problems of environmental pollution caused by the difficult degradation of lignin in straw, the decline of ancient trees due to wood-rotting fungi, and the over-reliance on chemical pesticides for plant disease control.

Description

Technical Field

[0001] This invention relates to the field of microbial technology, specifically to a Trichoderma harzianum JT3 and its applications. Background Technology

[0002] The main factor affecting the natural degradation of straw is lignin, the main component of plant cell walls. Lignin is a complex, highly heterogeneous aromatic biopolymer. Based on different monomers, lignin can be divided into three types: G-lignin, formed by the polymerization of guaiacol (G) units from the precursor rosinol; S-lignin, formed by the polymerization of syringyl (S) units from the precursor sinaponicol; and H-lignin, formed by the polymerization of p-hydroxyphenyl (H) units from the precursor p-coumarol. These monomers are cross-linked through several types of covalent bonds, including aryl ether bonds and carbon-carbon bonds, such as phenylpropane β-aryl ether bonds (β-O-4), phenylcoumarin (β-5), pinoresinol (β-β), biphenyl, and bisbenzodioxane (5-hydroxyphenyl) heterocycles. 5) Diaryl ethers (5-O-4) and helicodiones (β-1). The cell walls of ancient trees are mainly composed of lignocellulose. Lignin is a complex, highly heterogeneous aromatic biopolymer that is difficult to degrade. Ancient trees have recently shown signs of decay. Fungi that can cause wood decay and decompose wood residues in ancient trees are collectively called wood-decaying fungi. Wood-decaying fungi enrich global biodiversity and have significant ecological and economic value. Several important reported bioactive functions include antitumor activity, immunomodulation, antibacterial activity, and biotoxicity. Discovering and utilizing microorganisms that efficiently degrade lignocellulose is a new pathway for obtaining enzyme resources, and this initiative has significant practical implications.

[0003] Biological control of plant diseases refers to a method of controlling plant diseases using beneficial microorganisms and their metabolites. The mechanisms of action of biological control include competition, antagonism, hyperparasitism, inducing plant resistance, and promoting plant growth. Biological control has the advantages of being environmentally friendly, highly sustainable, and contributing to the maintenance of ecological balance. Furthermore, reducing the use of chemical pesticides can lower risks to human health. In the area of ​​biocontrol fungi, *Trichoderma* (…) Trichoderma spp .) is currently the most studied biocontrol fungus, and the most common one is Trichoderma harzianum ( T. harzianum ), green Trichoderma ( T.viride ) and Trichoderma longifolia ( T. longibrachiatumTrichoderma, among others, exhibits excellent inhibitory effects against various pathogenic fungi. Several biocontrol agents based on Trichoderma have been developed and are widely used in agricultural production. Studies have found that Trichoderma harzianum T23 achieves over 70% control efficacy against Fusarium oxysporum and Fusarium rot in the field, and compared to carbendazim powder, it can increase the yield of medicinal herbs by up to 24.5%. Furthermore, Trichoderma harzianum shows significant inhibitory effects on tomato gray mold, leaf mold, wilt, and brown spot. Biocontrol bacteria are widely used in the field of biological control. Among them, Bacillus spp. (…) Bacillus Pseudomonas spp. has attracted much attention due to its strong biocontrol capabilities. Pseudomonas ) and Agrobacterium radioactivee ( Agrobacterium radiobacter Bacteria such as Bacillus subtilis N-18 also play an important role in biological control. Bacillus subtilis N-18 can inhibit the growth of mycelium of the pathogen causing tomato root rot. Biological control of plant diseases not only helps reduce chemical pesticide pollution and maintain the ecological balance of farmland, but also enhances crop disease resistance through microbial antagonism, thereby promoting the green transformation of agriculture and achieving increased yields and efficiency. Summary of the Invention

[0004] To address the aforementioned shortcomings in the existing technology, this invention provides a Trichoderma harzianum JT3 and its application, which effectively solves the problems of environmental pollution caused by the difficult degradation of lignin in straw, the decline of ancient trees due to wood-rotting fungi, and the over-reliance on chemical pesticides for the prevention and control of plant diseases.

[0005] To achieve the above objectives, the technical solution adopted by the present invention to solve its technical problem is: to provide a Trichoderma harzianum JT3, with the Latin name... Trichoderma harzianum JT3 was deposited on September 22, 2025 at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 20252082 and address: Wuhan University, Wuhan, China.

[0006] Furthermore, Trichoderma harzianum JT3 can be used to produce ethyl styraxate from corn stalks.

[0007] The above-mentioned Trichoderma harzianum JT3 is used in the degradation of lignin, cellulose and hemicellulose.

[0008] The above-mentioned application of Trichoderma harzianum JT3 in the preparation of lignocellulose degrading enzyme preparations.

[0009] Furthermore, the degrading enzymes are laccase, lignin peroxidase, manganese peroxidase, exoglucanase, β-glucosidase, and xylanase.

[0010] The above-mentioned application of Trichoderma harzianum JT3 in the preparation of biocontrol agents.

[0011] Furthermore, the active ingredient of the biocontrol agent is a metabolite of Trichoderma harzianum JT3.

[0012] The above-mentioned application of Trichoderma harzianum JT3 in the biological control of plant diseases.

[0013] The above-mentioned application of Trichoderma harzianum JT3 in inhibiting the growth of plant pathogenic fungi.

[0014] Furthermore, the plant pathogenic fungi include Fusarium oxysporum, Subspora, Staphylococcus aureus, Prunus spp., Blight, and Colletotrichum.

[0015] Furthermore, Trichoderma harzianum JT3 showed an inhibition rate of 54.75-58.03% against Fusarium oxysporum, 48.04-53.18% against Subspora, 72.12-77.78% against Staphylococcus aureus, 68.12-73.66% against Agrophytes sacchari, 68.19-70.67% against Bacillus thuringiensis, and 58.60-59.52% against Colletotrichum gloeosporioides.

[0016] In summary, the present invention has the following beneficial effects: 1. This invention screens and identifies *Trichoderma harzianum* JT3, a high-yield lignocellulase producer with antibacterial activity, from ancient trees. Applying *Trichoderma harzianum* JT3 to degrade agricultural straw and forestry timber lays the foundation for efficient and green agricultural waste treatment. Biological control is the greenest and most environmentally friendly method of pest control. Analyzing the antibacterial products produced by the strain during lignocellulosic degradation reveals that applying these products to agricultural production not only helps reduce chemical pesticide pollution and maintain the ecological balance of farmland, but also provides a theoretical basis and scientific evidence for the research and development of highly efficient and high-quality biocontrol agents. Furthermore, this invention conducts multi-omics analysis; the results of whole-genome and metabolomics analyses can provide a theoretical basis for the further efficient utilization of biomass resources.

[0017] 2. *Trichoderma harzianum* JT3 can produce enzymes that degrade lignin, such as laccase, lignin peroxidase, and manganese peroxidase, as well as enzymes that degrade cellulose and hemicellulose, such as exoglucanosidase, endoglucanosidase, β-glucosidase, and xylanase, possessing comprehensive lignocellulose degradation capabilities. Wheat straw treated with this strain for 15 days exhibits a broken waxy surface layer and a loosened tissue structure, providing a high-quality strain resource for the efficient and green treatment of agricultural straw, forestry timber, and other wastes. This effectively solves the resource waste and environmental pollution problems caused by the difficulty of natural straw degradation.

[0018] 3. Trichoderma harzianum JT3 exhibits significant broad-spectrum antibacterial effects, inhibiting six common plant pathogens, including Fusarium oxysporum, Colletotrichum gloeosporioides, and Staphylococcus aureus, with inhibition rates ranging from 50.61% to 74.95%. The inhibition rate against Staphylococcus aureus reaches as high as 74.95%, providing a highly efficient biocontrol strain for plant disease prevention and control, reducing the use of chemical pesticides. This invention expands the resource reserves of wood-rotting fungi in agricultural applications and provides a new direction for the exploration of functional bacteria. As a biocontrol strain, its application can reduce chemical pesticide pollution, maintain the ecological balance of farmland, and simultaneously enhance crop disease resistance through microbial antagonism, contributing to the green transformation of agriculture and achieving increased yield and efficiency.

[0019] 4. This invention clarified the genomic characteristics of the strain through whole-genome sequencing, annotated a large number of carbohydrate-active enzyme-related genes, and revealed the variation patterns of its differential metabolites through metabolomics analysis. This provides a solid theoretical foundation and scientific basis for further in-depth research on the degradation mechanism of the strain, the synthesis pathway of antibacterial metabolites, and the development of efficient and high-quality biocontrol agents.

[0020] 5. The Trichoderma harzianum JT3 provided by this invention can be used to produce ethyl scurvyate from corn straw. Ethyl scurvyate has antibacterial / antibiotic activity, antioxidant activity, anti-inflammatory activity, and antitumor / cytotoxicity. It has inhibitory effects on a variety of Gram-positive bacteria, can scavenge free radicals, has potential antioxidant effects, has shown anti-inflammatory potential in some studies, and has selective inhibitory effects on certain cancer cell lines. It is one of the candidate molecules for antitumor drug research. Attached Figure Description

[0021] Figure 1 The image shows the filter paper enzyme activity results of 43 fungal strains. Figure 2 This is a comparison of the decolorization of strain JT3 on aniline blue plates; Figure 3 Morphological characteristics of plant JT3; Figure 4 Phylogenetic tree diagram of strain JT3; Figure 5 The graph shows the results of the determination of lignocellulase activity. Figure 6 The graph shows the results of the determination of lignocellulase activity. Figure 7 The graph shows the results of the determination of lignocellulase activity. Figure 8 Electron micrograph of the surface morphology of wheat straw; Figure 9 A gene family of carbohydrate-active enzymes and a distribution map of enzyme genes; Figure 10 A circular diagram showing the metabolite class composition of strain JT3 in cationic mode; Figure 11 A circular diagram showing the metabolite composition of strain JT3 under anion mode; Figure 12 The image shows the antagonistic effect of strain JT3 against six pathogens. Detailed Implementation

[0022] The principles and features of this invention are described below. The examples given are for illustrative purposes only and are not intended to limit the scope of the invention. Unless otherwise specified in the examples, conventional conditions or conditions recommended by the manufacturer should be followed. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0023] Example 1: Screening of Lignocellulose-Degrading Strains The pathogens selected for the experiment were: Fusarium oxysporum (… Fusarium oxysporum ), Colloidal anthrax ( Colletotrichum gloeosporioides ), Botrytis cinerea ( Botrytis cinerea ), Subspora spp. ( Didymella sp. Staphylococcus aureus ( Botryosphaeria dothidea ), Polychaete spp. ( Pestalotiopsis ) and Sweet Cherry Intercalated Scale ( Diaporthe The 48 strains, including 42 strains selected from the decay of ancient trees, were isolated, identified and preserved by the Plant Pathology Laboratory of Beijing University of Agriculture.

[0024] Enzyme-producing culture medium: 10 g / L milled wheat straw as carbon source; 0.2 g / L ammonium tartrate; 2 g / L KH₂PO₄; 0.71 g / L MgSO₄·7H₂O; 0.1 g / L CaCl₂; and 70 mL trace element solution. The trace element solution contained 1 g / L NaCl; 0.184 g / L CoCl₂·6H₂O; 0.1 g / L FeSO₄·7H₂O; 0.1 g / L ZnSO₄·7H₂O; 0.1 g / L CuSO₄; 0.01 g / L H₃BO₃; 0.01 g / L Na₂MoO₄·2H₂O; 0.01 g / L KAl(SO₄)₂·12H₂O; and 1.5 g / L N-triacetic acid.

[0025] Potato glucose agar medium (PDA medium): 200.0 g potato, 20.0 g glucose, 15.0-20.0 g agar powder, 1000 mL deionized water, pH 7.0.

[0026] (1) Filter paper enzyme activity assay A total of 43 fungal strains were isolated and identified from decaying parts of ancient trees and collected from the plant pathology laboratory of Beijing University of Agriculture. Filter paper enzyme activity experiments were conducted to observe whether any strains possessed the ability to degrade cellulose.

[0027] The preserved bacterial strain was first activated and cultured, and then vigorous mycelia were selected and inoculated into liquid culture medium to prepare seed culture. The seed culture was inoculated at a rate of 5% into a 250 mL Erlenmeyer flask containing 50 mL of initial enzyme-producing medium and incubated at 28℃ and 180 rpm in a constant temperature shaker. The culture was centrifuged at 10000 r / min for 10 min, and the supernatant was collected as crude enzyme solution. The enzyme activity was measured using filter paper. 50 ± 1 mg of Waterman™ No. 1 filter paper was placed at the bottom of a centrifuge tube, along with 1 mL of pH 6.0 buffer and 500 μL of crude enzyme solution. The mixture was thoroughly mixed and reacted at 50℃ for 1 h. The reaction was terminated by adding 2 mL of DNS, boiling for 10 min, and allowing it to cool. Distilled water was then added to bring the volume to 12.5 mL, and the mixture was shaken well. The OD value was measured at a wavelength of 540 nm. The results are as follows: Figure 1 As shown.

[0028] Depend on Figure 1 It can be seen that among the 43 fungal strains tested, strain JT3 had significantly higher filter paper enzyme activity than other strains.

[0029] (2) Aniline blue plate initial screening Strain JT3 was initially screened using aniline blue agar plates. The isolated, purified strain, stored in a refrigerator, was picked from the slant and transferred to PDA agar plates for activation. After incubation at 28°C for 7 days, mycelial discs were prepared at the edge of the colonies using a 10 mm diameter sterile punch. One mycelial disc was inoculated into the center of each PDA-aniline blue agar plate, with three replicates. The plates were incubated at 28°C for 5 days, and mycelial growth was observed daily, with the presence or absence of a discoloration zone recorded. Results are as follows: Figure 2 As shown, the inactivated fermentation broth was used as a control.

[0030] Depend on Figure 2 It can be seen that strain JT3 has a good lignin degradation ability.

[0031] Example 2: Strain Identification (1) Morphological observation Slides were prepared using the slide insertion method. The prepared slides were then observed under optical and electron microscopes to record the morphology of fungal hyphae, and the attachment, morphology, and size of conidia. The morphology of strain JT3 is shown below. Figure 3 As shown. Figure 3 In the figure, A represents the morphological characteristics of the strain after 3 days of growth, B represents the morphological characteristics of the strain after 6 days of growth, and C represents the morphological characteristics of strain JT3 under a 3300× electron microscope.

[0032] Depend on Figure 3 It is known that the initial hyphae of strain JT3 are slender, colorless, septate, and highly branched. Conidiophores arise from the lateral branches of the hyphae, opposite or alternate, and generally branch 2-3 times. The peduncles bearing conidia are bottle-shaped or conical. In PDA medium, the colonies are white and fluffy after 3 days, and the spore colonies turn dark green after 3 days.

[0033] (2) Molecular biological identification Genomic DNA was extracted from strain JT3, and 16S rDNA was amplified by PCR using universal fungal primers ITS1 and ITS4. The PCR products were sent to Sangon Biotech (Shanghai) Co., Ltd. for sequencing. BLAST alignment of the sequencing results was performed, and phylogenetic analysis of the obtained homologous sequences was conducted using MEGA 11.0 software. The results are as follows: Figure 4 As shown.

[0034] Depend on Figure 4 It can be seen that strain JT3 and Trichoderma harzianum The strain JT3 was identified as Trichoderma harzianum based on its morphological characteristics and its closest phylogenetic relationship with Trichoderma harzianum.

[0035] Experimental Example: Determination of the Activity of Lignocellulose Degrading Enzymes Example 3: Determination of Lignocellulase Activity Laccase activity was determined by monitoring the oxidation of ABTS at 420 nm using ABTS as a substrate. Lignin peroxidase activity was determined by monitoring the oxidation of resveratrol to veratral at 310 nm using resveratrol as a substrate. Manganese peroxidase activity was determined using MnSO4 as a substrate. Exoglucanase activity was determined using a micro-method with pNPC as a substrate. Endoglucanase activity was determined using the DNS method with glucose as a substrate. β-glucosidase activity was determined using a micro-method with pNPG as a substrate. Xylanase activity was determined using the DNS method with xylan as a substrate. Results are as follows: Figures 5-7 As shown, Figure 5 The left image shows laccase activity, and the right image shows lignin peroxidase activity. Figure 6 In the middle, the left image shows the activity of manganese peroxidase, and the right image shows the activity of exoglucanase. Figure 7 In the middle, the left image shows β-glucosidase activity, and the right image shows xylanase activity.

[0036] Depend on Figures 5-7 It was found that strain JT3 could produce six lignocellulases: laccase, manganese peroxidase, and lignin peroxidase, which can degrade lignin; exoglucanase and β-glucosidase, which can degrade cellulose. The presence of xylanase, which can degrade hemicellulose, indicates that strain JT3 has a good degradation effect.

[0037] Example 4: Treatment of wheat straw with strain JT3 The surface structure of wheat straw treated with and untreated by Trichoderma harzianum strain JT3 was observed using scanning electron microscopy. The results are as follows: Figure 8 As shown, Figure 8 In the figure, A represents the surface morphology of untreated wheat straw, and B represents the surface morphology of wheat straw treated with strain JT3.

[0038] Depend on Figure 8 It can be seen that the surface of straw that has not been fermented by the strain is smooth and the structure is intact. After being fermented by Trichoderma harzianum JT3 for 15 days, the surface wax layer of straw is destroyed and the structure is loose.

[0039] Example 5: Whole genome analysis of strain JT3 Fungal DNA was extracted using the method described in the rapid fungal genomic DNA extraction kit (Beijing Adley Biotechnology Co., Ltd.), and the samples were sent to Huaguang Gene Biotechnology Co., Ltd. for whole genome sequencing.

[0040] JT3 genome sequencing was performed using the third-generation PacBio revio platform. First, the sample concentration was determined. Then, agarose gel electrophoresis was used to detect the purity and integrity of the DNA. Finally, library construction and testing were performed using the DNPSEQ and PacBio platforms.

[0041] The fragments were precisely fragmented using Megauptor, then sorted and precisely recovered using SageELF, resulting in a library with very high fragment size consistency (HiFi library). After PacBio sequencing, each double-stranded insert fragment could be sequenced multiple times in a loop, and high-fidelity sequencing results were obtained through self-correction, with a sequencing accuracy of ≥99%.

[0042] After assembly, the components of the sample genome were analyzed. Genemarkes and Augustus software were used to predict coding genes. rRNA was located by alignment with an rRNA library or by predicting rRNA using RNAmmer software. tRNA regions and secondary structures were predicted using tRNAscan software. sRNA was obtained by alignment with the Rfam database using Infernal software. Transposon sequences were then located by alignment of the assembly results with known transposon sequence libraries and the Denovo method. Specifically, transposons were predicted using RepeatMasker, RepeatProteinMasker, and Denovo software; tandem repeat sequences were predicted using TRF (TandemRepeatFinder) software.

[0043] (1) Basic characteristics of the genome The whole genome sequencing results of Trichoderma harzianum JT3 are shown in Table 1.

[0044] Table 1. Statistical data of assembly results

[0045] As shown in Table 1, the total assembly length was 40,090,270 bp, with 23 scaffolds and 23 contigs. The GC content was 48.39%, the N5O value was 3,502,741 bp, the N90 value was 1,779,488 bp, the maximum assembly length was 4,438,954 bp, and the minimum assembly length was 12,462 bp.

[0046] A total of 495 genes were annotated using a network database of automatically annotated carbohydrate-active enzymes, and the results are as follows: Figure 9 As shown. Figure 9 In the image, the left figure shows the gene family of carbohydrate-active enzymes, and the right figure shows the distribution of enzyme genes.

[0047] Depend on Figure 9 It was found that there were 94 auxiliary oxidoreductases (AAs), 3 carbohydrate binding modules (CBMs), 24 carbohydrate esterases (CEs), 267 glycoside hydrolases (GHs), 99 glycosyltransferases (GTs), and 8 polysaccharide lyases (PLs). The first three gene families were identified as AA7 (36 genes), GH18 (25 genes), GH3 (18 genes), and AA3_2 (17 genes). Laccase genes (EC.1.10.3.2) were predicted in AA1_2 (2 genes), AA1 (6 genes), and AA1-3 (1 gene); lignin peroxidase (EC.1.11.1.14) and manganese peroxidase (EC.1.11.1.13) were predicted among the 7 genes in AA2; and endoglucanase (EC 3.2.1.4), exoglucanase (EC 3.2.1.91), β-glucosidase (EC 3.2.1.21), and xylanase (EC 3.2.1.8) were annotated in multiple GH families.

[0048] Example 6: Non-target metabolomics analysis High-performance liquid chromatography (HPLC) conditions: All samples were acquired by the LC-MS system according to instrument instructions. Analytical conditions were as follows: Ultra-high performance liquid chromatography (UHPLC): Column: Waters ACQUITY UPLC HSS T3 (1.8 μm, 2.1 mm × 100 mm); Column temperature: 40 °C; Flow rate: 0.40 mL / min; Injection volume: 4 μL; Solvent system: water (containing 0.1% formic acid): acetonitrile (containing 0.1% formic acid); Sample determination used a gradient program, with initial conditions of 95% A, 5% B. Within 5 minutes, the linear gradient changed to 35% A, 65% B; within 1 minute, the linear gradient changed to 1% A, 99% B, and held for 1.5 minutes. Subsequently, within 0.1 minutes, it was adjusted to 95% A, 5.0% B, and held for 2.4 minutes.

[0049] Mass spectrometry conditions: Analyst TF 1.7.1 software (Sciex, Concord, Ontario, Canada) was used for data acquisition in Information Dependent Acquisition (IDA) mode. Ion source parameters were set as follows: Ion source gas 1 (GAS1) 50 psi; ion source gas 2 (GAS2) 60 psi; curtain gas (CUR) 35 psi; temperature (TEM) 550 ℃; declustering voltage (DP) 80 V and -80 V in positive and negative ion modes, respectively; ion spray voltage (ISVF) 5500 V in positive ion mode and -4500 V in negative ion mode. Time-of-flight mass spectrometry scan parameters were set as follows: mass range 50-1250 Da; accumulation time 200 ms; dynamic background subtraction enabled. The product ion scanning parameters were set as follows: mass range 50-1250 Da; cumulative time 40 ms; collision energies of 30 V and -30 V in positive and negative ion modes, respectively; collision energy broadening 15; unit resolution; charge state 1-1; intensity 100 cps; exclusion of isotopes within 4 Da; mass tolerance 50 mDa; maximum number of candidate ions monitored per cycle 12. The metabolite composition ring diagram of strain JT3 is shown below. Figure 10 and Figure 11 As shown. Among them, Figure 10 Results in cationic mode. Figure 11 The results are in anion mode.

[0050] Depend on Figure 10 and Figure 11Based on non-targeted metabolomics technology, a total of 1618 metabolites were detected in the default mode, including 1005 metabolites in the cationic mode and 613 metabolites in the anionic mode. In the cationic mode, amino acids and their derivatives accounted for the largest proportion at 29.75%, followed by benzene and its substituted derivatives at 8.42%. In the anionic mode, amino acids and their derivatives accounted for the largest proportion at 14.84%, followed by benzene and its substituted derivatives at 11.84%. Analysis of these products revealed that JT3 has the ability to synthesize ethyl serotonate (a phenolic acid).

[0051] Example 7: Determination of the antibacterial spectrum of strain JT3 Antagonistic plate experiments were conducted on Trichoderma harzianum JT3 and six pathogenic fungi preserved in the Plant Pathology Laboratory of Beijing Agricultural College, such as... Figure 12 As shown in Table 2, the inhibition rate of Trichoderma harzianum JT3 against plant pathogenic fungi is shown in Table 2.

[0052] Table 2. Inhibition rate of Trichoderma harzianum JT3 against plant pathogenic fungi

[0053] Depend on Figure 12 As shown in Table 2, the inhibitory effect of Trichoderma harzianum JT3 on different types of pathogens varies. The inhibition rate of *Botrytis cinerea* reached 74.95%, while that of *Botrytis cinerea* was 70.89%, and the inhibition rate of the remaining pathogens was 50.61-69.42%.

[0054] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A type of Trichoderma harzianum JT3, named in Latin... Trichoderma harzianum JT3 was deposited on September 22, 2025 at the China Center for Type Culture Collection (CCTCC) with accession number CCTCC NO: M 20252082 and address: Wuhan University, Wuhan, China.

2. The *Trichoderma harzianum* JT3 as described in claim 1, characterized in that, The Trichoderma harzianum JT3 can be used to produce ethyl styraxate from corn stalks.

3. The application of Trichoderma harzianum JT3 as described in claim 1 in the degradation of lignin, cellulose and hemicellulose.

4. The application of Trichoderma harzianum JT3 as described in claim 1 in the preparation of lignocellulose degrading enzyme preparations.

5. The application as described in claim 4, characterized in that, The degrading enzymes are laccase, lignin peroxidase, manganese peroxidase, exoglucanase, β-glucosidase, and xylanase.

6. The application of Trichoderma harzianum JT3 as described in claim 1 in the preparation of biocontrol agents.

7. The application as described in claim 6, characterized in that, The active ingredient of the biocontrol agent is a metabolite of Trichoderma harzianum JT3.

8. The application of Trichoderma harzianum JT3 as described in claim 1 in the biological control of plant diseases.

9. The application of Trichoderma harzianum JT3 as described in claim 1 in inhibiting the growth of plant pathogenic fungi.

10. The application as described in claim 9, characterized in that, The plant pathogenic fungi mentioned are Fusarium oxysporum, Subspora, Staphylococcus aureus, Prunus sacchariformis, Blight-causing fungi, and Colletotrichum gloeosporioides.

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