Compositions containing prebiotics, methods of making and use in skin conditioning products

Abstract

This invention provides a prebiotic-containing composition, its preparation method, and its application in skin-conditioning products, relating to the field of prebiotic product technology. The composition comprises, by weight, 30-50 parts xylooligosaccharides, 20-35 parts Sophora japonica flowers, 10-20 parts Pleurotus ostreatus, 10-20 parts astaxanthin, and 4-8 parts PQQ. The Sophora japonica flowers require enzymatic hydrolysis: the powder is sequentially hydrolyzed using β-glucosidase and papain, followed by ultrasonic extraction. The resulting extract is then dried to obtain the Sophora japonica flower extract. The components of this invention work synergistically to deliver significant anti-aging, antioxidant, elasticity-enhancing, fibroblast-increasing, and skin-conditioning effects, making it suitable for use in food or cosmetic preparations.

Description

Technical Field

[0001] This invention belongs to the field of prebiotic product technology, specifically relating to prebiotic-containing compositions, preparation methods, and their application in skin conditioning products. Background Technology

[0002] Aging, also known as aging, is mainly divided into intrinsic and extrinsic aging of the skin. Intrinsic aging, or natural aging, is caused by internal factors within the body. It typically refers to the decline in bodily functions, internal homeostasis, and stress response following normal biological maturation, leading to gradual degenerative changes in skin structure and composition. Intrinsic aging is primarily manifested as wrinkles and sagging skin. Extrinsic aging, on the other hand, is mainly caused by environmental factors, including sun exposure, smoking, and exposure to harmful chemicals. Extrinsic aging is characterized by deep wrinkles, thickened stratum corneum, a dull, lackluster appearance, pigmentation, a yellowish tinge, sagging, and age spots.

[0003] How to effectively remove free radicals from the skin while enhancing the skin's own antioxidant capacity, reducing the formation of fine lines, removing wrinkles, and achieving multiple anti-aging goals is a technical challenge in this field.

[0004] Chinese invention patent CN115501157A discloses a facial mask for acne treatment, acne scar repair, anti-aging, moisturizing, and whitening, and its preparation method. The ingredients include: dragon's blood powder, centella asiatica extract, acetyl hexapeptide-3, acetyl hexapeptide-6, carnosine, vitamin C ethyl ether, astaxanthin, lycopene, prickly pear extract, chrysanthemum extract, lavender extract, rosemary extract, aloe vera extract, 1,2-hexanediol, sodium hyaluronate, grape seed oil, glycerin, niacinamide, arbutin, rose extract, sodium carboxymethyl cellulose, xanthan gum, sorbitan sesquioleate, disodium EDTA, potassium sorbate, citric acid, and deionized water. This facial mask has anti-aging, moisturizing, and whitening effects.

[0005] Chinese invention patent CN105055251A discloses an anti-aging cosmetic and its preparation method, comprising the following components by mass percentage: 10%-20% spirulina phycocyanin, 1%-2% Haematococcus pluvialis extract, 2%-5% vitamin E, 5%-8% sea buckthorn oil, 25%-35% oil phase raw materials, and 30%-55% aqueous phase raw materials, with the sum of all components being 100%. The oil phase raw materials are melted and thoroughly stirred. The aqueous phase raw materials are sterilized by heating while stirring. The oil and aqueous phases are emulsified after being uniformly dispersed. Then, spirulina phycocyanin, Haematococcus pluvialis extract, vitamin E, and sea buckthorn oil are added. After stirring evenly, the mixture is allowed to cool naturally to obtain the anti-aging cosmetic. The anti-aging cosmetic provided by this invention has strong permeability, can improve keratinization, and has moisturizing effects.

[0006] Chinese invention patent CN107549812A discloses a beauty and anti-aging food product made from acerola cherry extract, Haematococcus pluvialis, and fish collagen peptides, and its preparation method. The beauty and anti-aging food product comprises the following ingredients in parts by weight: 20-30 parts acerola cherry extract, 20-30 parts Haematococcus pluvialis powder, and 40-60 parts fish collagen peptides. This invention's food product has beauty and anti-aging effects. Using Haematococcus pluvialis, acerola cherry extract, and fish collagen peptides as raw materials, it promotes skin cell regeneration, slows down skin cell aging, replenishes and maintains skin moisture, reduces skin pigmentation and deposition, and achieves effects such as increasing skin elasticity, anti-wrinkle and anti-aging, smoothing fine lines, fading spots, and whitening and brightening the skin.

[0007] However, many food and cosmetic products on the market that claim to have anti-aging and antioxidant effects contain a large number of chemical additives, resulting in inconsistent quality and poor efficacy; the various ingredients also fail to achieve a good synergistic effect. Furthermore, since skin aging is mostly the result of the combined effects of endogenous and exogenous factors, improving skin texture solely through topical cosmetics or oral food has a weak effect and takes a long time to materialize.

[0008] Therefore, there is a need to develop a new composition that is green, free of chemical reagents, non-irritating, and highly effective in anti-aging, anti-oxidation, promoting skin metabolism, and improving skin texture. This composition can be used not only in cosmetics but also in food, achieving the dual benefits of internal and external use. Summary of the Invention

[0009] This invention addresses the problems existing in the prior art by providing a prebiotic-containing composition, its preparation method, and its application in skin conditioning products. The composition is formulated using xylooligosaccharides, sophora japonica flowers, concentrated pleurum chinense powder, astaxanthin, and PQQ (pyrroloquinoline quinone disodium salt). The resulting composition has significant anti-aging, antioxidant, wrinkle-removing, and skin-conditioning effects.

[0010] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0011] First, the present invention provides a composition containing prebiotics, comprising, by weight, the following components: 30-50 parts xylooligosaccharide (XOS), 20-35 parts Sophora japonica flower, 10-20 parts Pleurotus ostreatus, 10-20 parts astaxanthin and 4-8 parts PQQ; When preparing the composition, the Sophora japonica flower components need to undergo enzymatic hydrolysis. Specifically, the Sophora japonica flower powder is enzymatically hydrolyzed with β-glucosidase and papain in sequence, and then extracted by ultrasonication. The resulting extract is then dried to obtain the Sophora japonica flower extract.

[0012] Preferably, the composition comprises, by weight, the following components: 35-45 parts xylooligosaccharide, 25-32 parts Sophora japonica flower, 13-17 parts Pleurotus ostreatus, 13-17 parts astaxanthin and 5-6 parts PQQ.

[0013] More preferably, the composition comprises, by weight, 40 parts xylooligosaccharide, 28 parts Sophora japonica flower, 15 parts Pleurotus ostreatus, 15 parts astaxanthin and 5.5 parts PQQ.

[0014] Preferably, the golden top pine mushroom is pulverized powder or concentrated golden top pine mushroom powder.

[0015] Preferably, the astaxanthin is not limited to Haematococcus pluvialis astaxanthin.

[0016] Preferably, the mass ratio of β-glucosidase to papain is 30-50:1.

[0017] More preferably, the mass ratio of β-glucosidase to papain is 40:1.

[0018] Preferably, the β-glucosidase has an enzyme activity of 1000-2000 U / g; and the papain has an enzyme activity of 100-300 U / mg.

[0019] More preferably, the β-glucosidase has an enzyme activity of 1100 U / g; and the papain has an enzyme activity of 200 U / mg.

[0020] Preferably, the conditions for the enzymatic hydrolysis are: (a) when using β-glucosidase for enzymatic hydrolysis, the hydrolysis temperature is 40-60℃, the hydrolysis pH is 4.5-5.5, and the hydrolysis time is 6-12h; (b) when using papain for enzymatic hydrolysis, the hydrolysis temperature is 55-65℃, the hydrolysis pH is 6.0-7.5, and the hydrolysis time is 4-8h.

[0021] More preferably, the conditions for the enzymatic hydrolysis are: (a) when using β-glucosidase for enzymatic hydrolysis, the hydrolysis temperature is 45-55℃, the hydrolysis pH is 4.5-5.0, and the hydrolysis time is 8-10h; (b) when using papain for enzymatic hydrolysis, the hydrolysis temperature is 55-60℃, the hydrolysis pH is 6.5-7.0, and the hydrolysis time is 6-8h.

[0022] More preferably, the conditions for the enzymatic hydrolysis are: (a) when using β-glucosidase for enzymatic hydrolysis, the hydrolysis temperature is 50°C, the hydrolysis pH is 4.8, and the hydrolysis time is 10h; (b) when using papain for enzymatic hydrolysis, the hydrolysis temperature is 60°C, the hydrolysis pH is 6.8, and the hydrolysis time is 6h.

[0023] Preferably, the method for preparing the Sophora japonica flower extract is as follows: (1) Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis. The enzymatic hydrolysis temperature is 40-60℃, the enzymatic hydrolysis pH is 4.5-5.0, and the enzymatic hydrolysis time is 6-12h to obtain the first enzymatic hydrolysate. (2) Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.0-7.5, and carry out the second enzymatic hydrolysis at a temperature of 55-65℃ for 4-8 hours to obtain the enzymatic hydrolysate mixture; The amount of β-glucosidase added is 5‰-15‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 30-50:1. (3) The enzymatically hydrolyzed mixture was subjected to ultrasonic treatment for 5-10 minutes, filtered, and the filtrate was dried to obtain Sophora japonica extract.

[0024] More preferably, in step (1), the weakly acidic aqueous solution is selected from at least one of citric acid aqueous solution, acetic acid aqueous solution, and malic acid aqueous solution.

[0025] More preferably, in step (2), the components used to adjust the pH are not limited to sodium phosphate, sodium acetate, sodium carbonate, sodium hydroxide, or potassium hydroxide.

[0026] More preferably, the method for preparing the Sophora japonica extract is as follows: (1) Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis. The enzymatic hydrolysis temperature is 45-55℃, the enzymatic hydrolysis pH is 4.5-5.0, and the enzymatic hydrolysis time is 8-10h to obtain the first enzymatic hydrolysate. (2) Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.5-7.0, and carry out the second enzymatic hydrolysis at a temperature of 55-60℃ for 6-8 hours to obtain the enzymatic hydrolysate mixture; The amount of β-glucosidase added is 8‰-12‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 35-45:1. (3) The enzymatically hydrolyzed mixture was subjected to ultrasonic treatment for 5-10 minutes, filtered, and the filtrate was dried to obtain Sophora japonica extract.

[0027] More preferably, the method for preparing the Sophora japonica extract is as follows: (1) Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis at a temperature of 50℃, a pH of 4.8, and a time of 10h to obtain the first enzymatic hydrolysate. (2) Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.8, and carry out the second enzymatic hydrolysis at 60℃ for 6 hours to obtain the enzymatic hydrolysate mixture; The amount of β-glucosidase added is 10‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 40:1. (3) The enzymatically hydrolyzed mixture was subjected to ultrasonic treatment for 8 minutes, filtered, and the filtrate was dried to obtain Sophora japonica extract.

[0028] Then, the present invention provides a method for preparing the above composition, comprising the steps of: S1. Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis. The enzymatic hydrolysis temperature is 40-60℃, the enzymatic hydrolysis pH is 4.5-5.0, and the enzymatic hydrolysis time is 6-12h to obtain the first enzymatic hydrolysate. S2. Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.0-7.5, and carry out the second enzymatic hydrolysis at a temperature of 55-65℃ for 4-8 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 5‰-15‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 30-50:1. S3. The enzymatically hydrolyzed mixture is ultrasonicated for 5-10 minutes, filtered, and the filtrate is dried to obtain Sophora japonica extract. S4. Mix xylooligosaccharides, sophora japonica extract, pleurotus ostreatus, astaxanthin, and PQQ to obtain a composition.

[0029] Preferably, the method for preparing the composition includes the following steps: S1. Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis. The enzymatic hydrolysis temperature is 45-55℃, the enzymatic hydrolysis pH is 4.5-5.0, and the enzymatic hydrolysis time is 8-10h to obtain the first enzymatic hydrolysate. S2. Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.5-7.0, and carry out the second enzymatic hydrolysis at a temperature of 55-60℃ for 6-8 hours to obtain an enzymatic hydrolysate mixture. The amount of β-glucosidase added is 8‰-12‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 35-45:1. S3. The enzymatically hydrolyzed mixture is ultrasonicated for 5-10 minutes, filtered, and the filtrate is dried to obtain Sophora japonica extract. S4. Mix xylooligosaccharides, sophora japonica extract, pleurotus ostreatus, astaxanthin, and PQQ to obtain a composition.

[0030] More preferably, the method for preparing the composition includes the following steps: S1. Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis at a temperature of 50℃, a pH of 4.8, and a time of 10 hours to obtain the first enzymatic hydrolysate. S2. Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.8, and carry out the second enzymatic hydrolysis at 60℃ for 6 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 10‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 40:1. S3. The enzymatically hydrolyzed mixture was ultrasonicated for 8 minutes, filtered, and the filtrate was dried to obtain Sophora japonica extract. S4. Mix xylooligosaccharides, sophora japonica extract, pleurotus ostreatus, astaxanthin, and PQQ to obtain a composition.

[0031] Preferably, the mixing described in step S4 is carried out at room temperature.

[0032] Finally, the present invention provides the use of the above composition in the preparation of food and / or cosmetics.

[0033] Preferably, the food has the effects of anti-aging, anti-oxidation, improving skin elasticity, improving acne, improving skin moisture, regulating intestinal flora, and lubricating the intestines and relieving constipation.

[0034] Preferably, the cosmetic is a cosmetic with anti-aging, antioxidant, skin elasticity-enhancing, acne-improving, and skin moisture-enhancing effects.

[0035] Preferably, the cosmetics are not limited to essence water, essence liquid, essence lotion, emulsion, face cream, face mask, isolation cream, sunscreen, hand cream, and facial cleanser.

[0036] Compared with the prior art, the present invention has the following beneficial effects: 1. The composition of the present invention, through the synergistic use of xylooligosaccharides, Sophora japonica flowers, Pleurotus ostreatus, astaxanthin, and PQQ, delivers significant anti-aging, antioxidant, and elasticity-enhancing effects, increases the number of fibroblasts, and regulates skin texture. Specifically, Pleurotus ostreatus, astaxanthin, PQQ, and Sophora japonica flower extracts synergistically enhance the anti-aging effect.

[0037] 2. In the composition of the present invention, the use of prebiotic xylooligosaccharides can stimulate the expression of epidermal growth factor, promote epidermal cell renewal and repair of damaged tissue, and promote collagen formation; xylooligosaccharides can regulate the skin microbiome, inhibit the activity of Cutibacterium in the skin, and help improve skin condition, regulate skin texture, shrink facial pores and improve skin aging problems; xylooligosaccharides interact with Sophora japonica extract, Pleurotus ostreatus, astaxanthin, and PQQ to bring about significant anti-aging, antioxidant, wrinkle removal, improved elasticity, skin texture regulation, acne improvement and skin moisture improvement effects.

[0038] 3. The present invention uses β-glucosidase and papain in sequence to enzymatically hydrolyze Sophora japonica flowers, which is beneficial to improve the dissolution of effective components in Sophora japonica flowers. The gentle treatment of Sophora japonica flowers can retain the activity of effective components, resulting in significantly improved anti-aging and antioxidant effects. Detailed Implementation

[0039] The following non-limiting embodiments are intended to enable those skilled in the art to gain a more comprehensive understanding of the present invention, but do not limit the invention in any way. The following content is merely an exemplary description of the scope of protection of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of protection of the present invention.

[0040] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0041] The present invention will be further described below by way of specific embodiments. Unless otherwise specified, all chemical reagents used in the embodiments of the present invention are obtained through conventional commercial means.

[0042] In this invention, the *Pleurotus ostreatus* used is *Pleurotus ostreatus* powder, specifically concentrated *Pleurotus ostreatus* powder. The concentrated *Pleurotus ostreatus* powder used can be a commercially available product or a product obtained through water extraction.

[0043] In this invention, the astaxanthin used is Haematococcus pluvialis astaxanthin, which can be a commercially available product or a product obtained by alcohol extraction from Haematococcus pluvialis.

[0044] In the following specific embodiments, the concentrated powder of *Pleurotus ostreatus* is the product obtained by water extraction of *Pleurotus ostreatus* fruiting bodies, followed by spray drying. The astaxanthin is *Haematococcus pluvialis* astaxanthin, obtained by alcohol extraction of *Haematococcus pluvialis*. The total content of xylobiose, xylotriose, and xylotetraose in the xylooligosaccharides is ≥80%, and the xylooligosaccharides are sourced from Henan Yichangqing Biotechnology Co., Ltd. (derived from corn cobs). The content of PQQ (disodium pyrroloquinoline quinone) (on a dry basis) is ≥98.0%. The mannose is purchased from Xuzhou Cyber ​​Biotechnology Co., Ltd. The CAS number of the lycopene is 502-65-8, and the chemical formula is C. 40 H 56 .

[0045] In the following specific embodiments, the enzymes are diluted before use to ensure that the enzyme activity is within the following ranges: the β-glucosidase activity is 1100 U / g; the bromelain activity is 200 U / mg; and the papain activity is 200 U / mg. Products from different manufacturers do not significantly affect the efficacy.

[0046] Example 1 A composition containing prebiotics comprises, by weight: 40 parts xylooligosaccharide, 28 parts Sophora japonica flower, 15 parts Pleurotus ostreatus concentrate, 15 parts Haematococcus pluvialis astaxanthin, and 5.5 parts PQQ.

[0047] Sophora japonica flowers were prepared into Sophora japonica flower extract. The preparation method was as follows: (1) Pulverize the Sophora japonica flowers, mix the Sophora japonica flower powder with β-glucosidase, add citric acid aqueous solution, carry out the first enzymatic hydrolysis, the enzymatic hydrolysis temperature is 50℃, the enzymatic hydrolysis pH is 4.8, the enzymatic hydrolysis time is 10h, and the first enzymatic hydrolysate is obtained; (2) The first enzymatic hydrolysate was mixed with papain, and the pH was adjusted to 6.8 with sodium phosphate. The second enzymatic hydrolysis was carried out at 60°C for 6 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 10‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 40:1. (3) The enzymatically hydrolyzed mixture was sonicated for 8 minutes, filtered, and the filtrate was freeze-dried to obtain Sophora japonica extract.

[0048] The composition was obtained by mixing xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ.

[0049] Example 2 A composition containing prebiotics comprises, by weight: 35 parts xylooligosaccharide, 25 parts Sophora japonica flower, 13 parts Pleurotus ostreatus concentrate powder, 13 parts Haematococcus pluvialis astaxanthin and 5 parts PQQ.

[0050] Sophora japonica flowers were prepared into Sophora japonica flower extract. The preparation method was as follows: (1) Pulverize the Sophora japonica flowers, mix the Sophora japonica flower powder with β-glucosidase, add citric acid aqueous solution, carry out the first enzymatic hydrolysis, the enzymatic hydrolysis temperature is 60℃, the enzymatic hydrolysis pH is 5, the enzymatic hydrolysis time is 6h, and the first enzymatic hydrolysate is obtained. (2) Mix the first enzymatic hydrolysate with papain, adjust the pH to 7.5 with sodium phosphate, and carry out the second enzymatic hydrolysis at 65℃ for 4 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 5‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 30:1. (3) The enzymatic hydrolysate was sonicated for 10 min, filtered, and the filtrate was freeze-dried to obtain Sophora japonica extract.

[0051] The composition was obtained by mixing xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ.

[0052] Example 3 A composition containing prebiotics comprises, by weight: 45 parts xylooligosaccharide, 32 parts Sophora japonica flower, 17 parts Pleurotus ostreatus concentrate, 17 parts Haematococcus pluvialis astaxanthin, and 6 parts PQQ.

[0053] Sophora japonica flowers were prepared into Sophora japonica flower extract. The preparation method was as follows: (1) Pulverize the Sophora japonica flowers, mix the Sophora japonica flower powder with β-glucosidase, add citric acid aqueous solution, carry out the first enzymatic hydrolysis, the enzymatic hydrolysis temperature is 40℃, the enzymatic hydrolysis pH is 4.5, the enzymatic hydrolysis time is 12h, and the first enzymatic hydrolysate is obtained; (2) The first enzymatic hydrolysate was mixed with papain, and the pH was adjusted to 6 with sodium phosphate. The second enzymatic hydrolysis was carried out at 55°C for 8 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 15‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 50:1. (3) The enzymatic hydrolysate was sonicated for 5 minutes, filtered, and the filtrate was freeze-dried to obtain Sophora japonica extract.

[0054] The composition was obtained by mixing xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ.

[0055] Example 4 A composition containing prebiotics comprises, by weight: 30 parts xylooligosaccharide, 20 parts Sophora japonica flower, 10 parts Pleurotus ostreatus concentrate powder, 10 parts Haematococcus pluvialis astaxanthin and 4 parts PQQ.

[0056] Sophora japonica flowers were prepared into Sophora japonica flower extract. The preparation method was as follows: (1) Pulverize the Sophora japonica flowers, mix the Sophora japonica flower powder with β-glucosidase, add citric acid aqueous solution, carry out the first enzymatic hydrolysis, the enzymatic hydrolysis temperature is 45℃, the enzymatic hydrolysis pH is 4.5, the enzymatic hydrolysis time is 10h, and the first enzymatic hydrolysate is obtained. (2) The first enzymatic hydrolysate was mixed with papain, and the pH was adjusted to 7.0 with sodium phosphate. The second enzymatic hydrolysis was carried out at 55°C for 8 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 12‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 35:1. (3) The enzymatic hydrolysate was sonicated for 5 minutes, filtered, and the filtrate was freeze-dried to obtain Sophora japonica extract.

[0057] The composition was obtained by mixing xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ.

[0058] Example 5 A composition containing prebiotics comprises, by weight: 50 parts xylooligosaccharide, 35 parts Sophora japonica flower, 20 parts Pleurotus ostreatus concentrate powder, 20 parts Haematococcus pluvialis astaxanthin, and 8 parts PQQ.

[0059] Sophora japonica flowers were prepared into Sophora japonica flower extract. The preparation method was as follows: (1) Pulverize the Sophora japonica flowers, mix the Sophora japonica flower powder with β-glucosidase, add citric acid aqueous solution, carry out the first enzymatic hydrolysis, the enzymatic hydrolysis temperature is 55℃, the enzymatic hydrolysis pH is 5.0, the enzymatic hydrolysis time is 8h, and the first enzymatic hydrolysate is obtained; (2) Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.5 with sodium phosphate, and carry out the second enzymatic hydrolysis at 60℃ for 6 hours to obtain the enzymatic hydrolysate mixture; The amount of β-glucosidase added is 8‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 45:1. (3) The enzymatic hydrolysate was sonicated for 10 min, filtered, and the filtrate was freeze-dried to obtain Sophora japonica extract.

[0060] The composition was obtained by mixing xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ.

[0061] Comparative Example 1 Unlike Example 1, the composition does not contain the component Haematococcus pluvialis astaxanthin.

[0062] A composition containing prebiotics comprises, by weight: 40 parts xylooligosaccharide, 28 parts Sophora japonica flower, 15 parts Pleurotus ostreatus concentrate powder and 5.5 parts PQQ.

[0063] The method for preparing Sophora japonica flower extract is the same as in Example 1.

[0064] The combination was prepared by mixing xylooligosaccharides, sophora japonica extract, pleurotus ostreatus concentrate, and PQQ.

[0065] Comparative Example 2 Unlike Example 1, the composition does not contain the component Pleurotus ostreatus concentrate.

[0066] A composition comprising, by weight, 40 parts xylooligosaccharide, 28 parts Sophora japonica flower, 15 parts Haematococcus pluvialis astaxanthin and 5.5 parts PQQ.

[0067] The method for preparing Sophora japonica flower extract is the same as in Example 1.

[0068] Xylooligosaccharides, Sophora japonica flower extract, Haematococcus pluvialis astaxanthin, and PQQ were mixed to obtain a composition.

[0069] Comparative Example 3 Unlike Example 1, xylooligosaccharides were replaced with oligomannose. Oligomannose, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ were mixed to obtain a composition.

[0070] Everything else is the same as in Example 1.

[0071] Comparative Example 4 Unlike Example 1, the astaxanthin from Haematococcus pluvialis was replaced with lycopene. Xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, lycopene, and PQQ were mixed to obtain the composition.

[0072] Everything else is the same as in Example 1.

[0073] Comparative Example 5 Unlike Example 1, the composition has a different component ratio.

[0074] A composition containing prebiotics comprises, by weight: 40 parts xylooligosaccharide, 8 parts Sophora japonica flower, 30 parts Pleurotus ostreatus concentrate powder, 8 parts Haematococcus pluvialis astaxanthin and 2.5 parts PQQ.

[0075] Sophora japonica flowers were prepared into Sophora japonica flower extract, and the preparation method was the same as in Example 1.

[0076] The composition was obtained by mixing xylooligosaccharides, Sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin, and PQQ.

[0077] Comparative Example 6 Unlike Example 1, the preparation method of the Sophora japonica extract is different.

[0078] Sophora japonica flowers were prepared into Sophora japonica flower extract. The preparation method was as follows: (1) Pulverize the Sophora japonica flowers, mix the Sophora japonica flower powder with bromelain, adjust the pH to 6.8 with sodium phosphate, carry out enzymatic hydrolysis, the enzymatic hydrolysis temperature is 60℃, and the enzymatic hydrolysis time is 6h to obtain the enzymatic hydrolysate; (2) Mix the enzymatic hydrolysate with β-glucosidase, add citric acid aqueous solution, and carry out enzymatic hydrolysis at 50℃, pH=4.8, and time for 10h to obtain the enzymatic hydrolysate mixture; The amount of β-glucosidase added is 10‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to bromelain is 40:1. (3) The enzymatically hydrolyzed mixture was sonicated for 8 minutes, filtered, and the filtrate was freeze-dried to obtain Sophora japonica extract.

[0079] Everything else is the same as in Example 1; xylooligosaccharide, sophora japonica extract, Pleurotus ostreatus concentrate, Haematococcus pluvialis astaxanthin and PQQ are mixed to obtain the composition.

[0080] Comparative Example 7 Unlike Example 1, the composition does not contain the components Pleurotus ostreatus concentrate and Haematococcus pluvialis astaxanthin.

[0081] The composition consists of the following components by weight: 40 parts xylooligosaccharide, 28 parts sophora japonica flower and 5.5 parts PQQ.

[0082] The preparation method for preparing Sophora japonica flower extract is the same as in Example 1.

[0083] The xylooligosaccharide, sophora japonica extract and PQQ were mixed to obtain a composition.

[0084] Experiment 1 Evaluation of superoxide anion radical scavenging capacity Experimental methods: (1) Using ethanol as a solvent, the compositions of Examples 1-5 and Comparative Examples 1-7 were respectively prepared into sample solutions with a mass concentration of 1%; (2) Take 4.5 mL of Tris-HCl buffer solution (0.05 mol / L, pH 8.2) and preheat it in a water bath at 25°C for 30 min; then add 1 mL of sample solution and 0.4 mL of pyrogallol solution (25 mmol / L), mix well, react in a water bath at 25°C for 5 min, and add 1 mL of HCl (8 mmol / L) to terminate the reaction; (3) Using Tris-HCl buffer solution as a reference, the absorbance value was measured at 299 nm; 1 mL of ethanol was used as a blank control group.

[0085] Superoxide anion free radical scavenging rate (%) = [1-(A / A0)]×100%, where A0 is the absorbance value of the blank control group and A is the absorbance value of the sample group.

[0086] The results of the superoxide anion free radical scavenging rate are shown in Table 1.

[0087] Table 1

[0088] In Table 1, each comparative example group is compared with the Example 1 group. # P < 0.05 ## P < 0.01, indicating a significant difference.

[0089] As can be seen from Table 1, the composition of the present invention can achieve a scavenging rate of 96.2%-99.3% against superoxide anion free radicals, proving that the composition of the present invention has good antioxidant properties.

[0090] Comparative examples and Comparative Examples 1, 2, and 7 show that in the composition of the present invention, each component is indispensable and interacts with the others. Astaxanthin and Pleurotus ostreatus synergistically enhance the scavenging rate of superoxide anion free radicals, thereby improving the antioxidant performance of the composition. Comparative examples and Comparative Examples 3-4 show that the prebiotics and active components in the composition cannot be arbitrarily replaced. Replacing any component will weaken the composition's ability to scavenge superoxide anion free radicals. The synergistic antioxidant performance of the various components in the composition of the present invention is more significant. Comparative examples and Comparative Example 5 show that only when the ratio is within the range of the present invention can a high antioxidant performance be achieved. Ratios outside the present invention reduce the composition's ability to scavenge superoxide anion free radicals. Comparative examples and Comparative Example 6 show that the extraction method of Sophora japonica extract has a significant impact on the scavenging rate of superoxide anion free radicals. The Sophora japonica extract obtained by stepwise enzymatic hydrolysis using a specific type of complex enzyme interacts with other components in the composition, resulting in a significantly enhanced antioxidant effect.

[0091] Experiment 2: Detection of fibroblast proliferation performance Experimental method: Fibroblasts in the logarithmic growth phase were subjected to a 1×10⁻⁶ thiocyanate incubation. 5Cells were seeded at a density of [number] cells / mL in 96-well plates and divided into 13 groups. Twelve groups corresponded to the compositions provided in Examples 1-5 and Comparative Examples 1-7, respectively, with the remaining group serving as a blank control group. The test samples (compositions from Examples 1-5 and Comparative Examples 1-7) were added to the cell wells to achieve a concentration of 5 μg / mL for the active ingredient in each composition. An equal volume of DMEM culture medium was added to the blank control group. The treated fibroblasts were incubated at 5% CO2 and 37°C for 72 hours under constant temperature and humidity. Cell counts were performed in each group using a cell counter, and the cell numbers were recorded.

[0092] The experiment was repeated three times, and the data were statistically analyzed to obtain the effect of the composition on fibroblast proliferation. The fibroblast number results are shown in Table 2.

[0093] Table 2

[0094] In Table 2, each comparative example group is compared with the group in Example 1. # P < 0.05 ## P < 0.01, indicating a significant difference.

[0095] As shown in Table 2, the compositions of the embodiments of the present invention can significantly promote the proliferation of fibroblasts, with the number of fibroblasts increasing by 70%-95%. The components in the compositions of the present invention work synergistically to effectively promote cell growth. In contrast, the growth of fibroblasts in Comparative Examples 1, 2, and 7 was significantly reduced. Furthermore, the technical effects show that astaxanthin and *Pleurotus ostreatus* have a synergistic effect in promoting fibroblast proliferation in the compositions of the present invention. In Comparative Examples 3 and 4, where the prebiotic component was replaced and the astaxanthin component was replaced, the growth of fibroblasts was also significantly reduced, indicating that each component in the compositions of the present invention is indispensable and works synergistically to significantly influence fibroblast proliferation. The comparison of the technical effects of the embodiments and Comparative Example 5 shows that when the proportions of each component are outside the range of the present invention, the proliferation of fibroblasts in the composition is limited. The comparison of the technical effects of the embodiments and Comparative Example 6 shows that the extraction method of *Sophora japonica* extract has a significant impact on the proliferation of fibroblasts. The present invention uses a specific type of compound enzyme for stepwise enzymatic hydrolysis of *Sophora japonica*, which can significantly increase the number of fibroblasts.

[0096] Experiment 3: Anti-aging animal experiments Mouse animal experiments: Seventy-seven 16-month-old female ICR mice, weighing 33-45g, were selected and routinely fed. The mice were randomly divided into 11 groups of 7 mice each: model control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Example 5 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 4 group, Comparative Example 5 group, and Comparative Example 7 group. The dosage of the gavage composition for the Example and Comparative Example groups was 0.1g / kg; the model control group was administered the same volume of physiological saline. Gavage was continued for 60 days. After 60 days of gavage, sufficient serum samples were collected by enucleation and stored at -20℃ for later use.

[0097] Serum MDA (malondialdehyde) levels, SOD (superoxide dismutase), and GSH-Px (glutathione peroxidase) activities were detected using a kit-based method.

[0098] The results of MDA content, SOD activity and GSH-Px activity are shown in Table 3.

[0099] Table 3

[0100] Table 3 shows the comparison between each experimental group and the model control group. △ P < 0.05 △△ P < 0.01 indicates a significant difference; compared with the Example 1 group, each comparative group... # P < 0.05 ## P < 0.01, indicating a significant difference.

[0101] As shown in Table 3, compared with the model control group, the composition of the present invention significantly reduced serum MDA content and significantly increased SOD and GSH-Px activities in the mouse anti-aging experiment, demonstrating significantly improved anti-aging and antioxidant properties. The anti-oxidative and anti-aging efficacy of the present invention is significantly superior to that of the comparative examples. Comparison with Comparative Examples 1, 2, 4, and 7 shows that specific components and proportions in the composition of the present invention have a significant impact on the technical effects. The technical effects of Comparative Examples 1, 2, and 7 demonstrate that *Pleurotus ostreatus* and astaxanthin in the composition of the present invention have a synergistic effect, resulting in significant anti-aging efficacy. Comparison with Comparative Example 5 shows that the specific proportions of each component in the composition of the present invention have significant antioxidant and anti-aging effects.

[0102] Experiment 4: Skin elasticity effect test Volunteers: 56 female volunteers aged 30-40 years were randomly divided into 8 groups of 7 people each. One group was the blank control group, which applied only ordinary moisturizing products (glycerin cosmetics). The remaining 7 groups were the experimental groups: Example 1, Example 4, Example 5, Comparative Example 1, Comparative Example 2, Comparative Example 3 and Comparative Example 7, which applied the corresponding composition (1g composition mixed with 2g water).

[0103] Experimental method: After washing your face every morning and evening, apply 1g of the corresponding composition to the entire face. After 2 days, observe the face for any redness or allergic reactions. Then, conduct an 8-week application experiment.

[0104] Testing instrument: Elasticity tester, used to test skin firmness and elasticity.

[0105] Test principle: Based on the principles of suction and stretching, a constant negative pressure is applied to the test skin in the vertical direction, and then the negative pressure is removed. The displacement change of the test skin is measured within a specified time period to obtain the skin elasticity characterization value. The R2 value represents the total elasticity of the skin; an increase in the R2 value indicates an increase in skin elasticity.

[0106] The results of the change rate of R2 value (△D56) after 8 weeks are shown in Table 4. △D56 (%) = (total skin elasticity on day 56 - total skin elasticity on day 0) / total skin elasticity on day 0 × 100%.

[0107] Table 4

[0108] In Table 4, compared with Group 1 of Example, each group... # P < 0.05 ## P < 0.01, indicating a significant difference.

[0109] As shown in Table 4, after using the composition of the present invention, the skin elasticity characterization value R2 increased by 13.30%-15.72% compared with before use (day 0). Compared with the comparative example and the blank control group, the skin elasticity of the present invention was significantly improved. The results indicate that the composition of the present invention has a significant effect on increasing skin elasticity and can effectively resist aging, regulate skin texture, and improve skin elasticity.

[0110] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, and is not intended to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions made by those skilled in the art to the technical solution of the present invention do not depart from the essence and scope of the technical solution of the present invention.

Claims

1. A composition comprising a prebiotic, characterized in that, By weight, the components include: 30-50 parts xylooligosaccharides, 20-35 parts Sophora japonica flowers, 10-20 parts Pleurotus ostreatus, 10-20 parts astaxanthin, and 4-8 parts PQQ. When preparing the composition, the Sophora japonica flower components need to undergo enzymatic hydrolysis. Specifically, the Sophora japonica flower powder is enzymatically hydrolyzed with β-glucosidase and papain in sequence, and then extracted by ultrasonication. The resulting extract is then dried to obtain the Sophora japonica flower extract.

2. The composition of claim 1, wherein, By weight, the components include: 35-45 parts xylooligosaccharides, 25-32 parts Sophora japonica flowers, 13-17 parts Pleurotus ostreatus, 13-17 parts astaxanthin, and 5-6 parts PQQ.

3. The composition according to claim 2, characterized in that, By weight, the components include: 40 parts xylooligosaccharide, 28 parts sophora japonica flower, 15 parts golden oyster mushroom, 15 parts astaxanthin and 5.5 parts PQQ.

4. The composition according to claim 1, characterized in that, The mass ratio of β-glucosidase to papain is 30-50:

1.

5. The composition according to claim 4, characterized in that, The mass ratio of β-glucosidase to papain is 40:

1.

6. The composition according to claim 1, characterized in that, The conditions for the enzymatic hydrolysis treatment are as follows: (a) when using β-glucosidase for enzymatic hydrolysis, the hydrolysis temperature is 40-60℃, the hydrolysis pH is 4.5-5.5, and the hydrolysis time is 6-12h; (b) when using papain for enzymatic hydrolysis, the hydrolysis temperature is 55-65℃, the hydrolysis pH is 6.0-7.5, and the hydrolysis time is 4-8h.

7. The composition according to claim 6, characterized in that, The conditions for the enzymatic hydrolysis treatment are as follows: (a) when using β-glucosidase for enzymatic hydrolysis, the hydrolysis temperature is 45-55℃, the hydrolysis pH is 4.5-5.0, and the hydrolysis time is 8-10h; (b) when using papain for enzymatic hydrolysis, the hydrolysis temperature is 55-60℃, the hydrolysis pH is 6.5-7.0, and the hydrolysis time is 6-8h.

8. The composition according to claim 1, characterized in that, The preparation method of the Sophora japonica extract is as follows: (1) Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis. The enzymatic hydrolysis temperature is 40-60℃, the enzymatic hydrolysis pH is 4.5-5.0, and the enzymatic hydrolysis time is 6-12h to obtain the first enzymatic hydrolysate. (2) Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.0-7.5, and carry out the second enzymatic hydrolysis at a temperature of 55-65℃ for 4-8 hours to obtain the enzymatic hydrolysate mixture; The amount of β-glucosidase added is 5‰-15‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 30-50:

1. (3) The enzymatically hydrolyzed mixture was subjected to ultrasonic treatment for 5-10 minutes, filtered, and the filtrate was dried to obtain Sophora japonica extract.

9. A method for preparing the composition according to any one of claims 1-8, characterized in that, Including the following steps: S1. Mix Sophora japonica powder with β-glucosidase, add a weakly acidic aqueous solution, and carry out the first enzymatic hydrolysis. The enzymatic hydrolysis temperature is 40-60℃, the enzymatic hydrolysis pH is 4.5-5.0, and the enzymatic hydrolysis time is 6-12h to obtain the first enzymatic hydrolysate. S2. Mix the first enzymatic hydrolysate with papain, adjust the pH to 6.0-7.5, and carry out the second enzymatic hydrolysis at a temperature of 55-65℃ for 4-8 hours to obtain the enzymatic hydrolysate mixture. The amount of β-glucosidase added is 5‰-15‰ of the mass of Sophora japonica flower powder, and the mass ratio of β-glucosidase to papain is 30-50:

1. S3. The enzymatically hydrolyzed mixture is ultrasonicated for 5-10 minutes, filtered, and the filtrate is dried to obtain Sophora japonica extract. S4. Mix xylooligosaccharides, sophora japonica extract, pleurotus ostreatus, astaxanthin, and PQQ to obtain a composition.

10. The use of the composition according to any one of claims 1-8 in the preparation of food and / or cosmetics, characterized in that, The food mentioned above has the effects of anti-aging, anti-oxidation, improving skin elasticity, improving acne, improving skin moisture, regulating intestinal flora, and lubricating the intestines and relieving constipation. The cosmetics mentioned are cosmetics with anti-aging, antioxidant, skin elasticity improvement, acne improvement, and skin moisture improvement effects.

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