Construction method and application of nicotiana benthamiana chassis for synthesizing isoflavone compounds

By co-expressing key enzyme genes in Tobacco Benzoinus to construct an isoflavone synthesis chassis, the high cost and environmental pollution problems of microbial platforms have been solved, achieving efficient synthesis of isoflavone compounds and high yield of various isoflavones, providing a green and sustainable production solution.

CN122278902APending Publication Date: 2026-06-26SOUTHERN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Patent Information

Application Number
CN202411927003.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-12-25
Publication Date
2026-06-26

AI Technical Summary

Technical Problem

Existing microbial biosynthesis platforms suffer from high costs, biosafety and environmental pollution issues, making it difficult to efficiently synthesize isoflavones. Furthermore, plant extraction and chemical synthesis are limited by low abundance and structural complexity.

Method used

In *Nicotiana benthamiana*, the encoding genes for phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), chalcone reductase (CHR), chalcone isomerase (CHI), isoflavone synthase (IFS), and 2-hydroxyisoflavone dehydratase (HID) were co-expressed using expression vectors to construct an isoflavone synthesis chassis. Isoflavone compounds were then synthesized via Agrobacterium-mediated transformation.

Benefits of technology

Large-scale and efficient synthesis of isoflavones has been achieved, with a significant increase in yield, including high production of various isoflavones such as daidzein, genistein, and puerarin. This addresses the shortcomings of the microbial platform and provides a green and sustainable production solution.

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Abstract

The invention relates to the technical field of biology, in particular to a construction method and application of a nicotiana benthamiana chassis for synthesizing isoflavone compounds. According to the method, an isoflavone synthesis upstream channel is reconstructed in the nicotiana benthamiana, a key rate-limiting enzyme for synthesizing isoflavone in the nicotiana benthamiana is further identified, isoenzyme screening is further performed, a plurality of key genes are co-expressed by using 2A peptide, and a flavonoid synthesis transcription factor is further introduced, so that the isoflavone is synthesized. The nicotiana benthamiana chassis for synthesizing the isoflavone compound is constructed. The nicotiana benthamiana chassis can be used for efficiently synthesizing daidzein and genistein. Meanwhile, on the basis of a built daidzein and genistein high-yield Bensi tobacco platform, various isoflavone compounds, such as daidzein, genistein, puerarin, formononetin, chickpein A and medipterocarpin, can be further synthesized, a road is opened up for industrialization of biosynthesis of various active isoflavone plants, and the method has wide application prospects. Therefore, the method has a wide application prospect.
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