A scientific research sample adding and cell culture microplate operation table

Abstract

This invention provides a research sample loading and cell culture microplate operating table, including a base, a folding support, and a sample loading plate positioning frame. The folding support is hinged to the base and the sample loading plate positioning frame at both ends. The top surface of the sample loading plate positioning frame has a slot for placing and positioning the sample loading plate. A built-in background light source within the positioning frame provides background light for the sample loading plate placed in the slot. A sample loading counting device, which can be stored on the top of the base, is hinged to one side of the base. This device includes sequentially hinged counting units, each including a counting base plate with multiple rubber protrusions. This research sample loading and cell culture microplate operating table allows for adjustment of the sample loading plate tilt angle, reducing the difficulty of observation and operational risks during sample loading; it enables timely and effective recording of sample loading, avoiding duplicate loading and omissions; and the addition of a backlight allows for adjustment of the light source color and brightness, overcoming the influence of experimental background, lighting, and the reagents themselves on observation.

Description

Technical Field

[0001] This invention relates to the field of medical research experimental equipment technology, specifically a research sample loading and cell culture microplate operating table. Background Technology

[0002] Microplates are commonly used equipment in medical research experiments, widely applied in cell culture, immunological detection, and monoclonal antibody preparation. Microplates are typically porous, transparent plates made of pure polystyrene, characterized by numerous and densely packed pores. When adding samples, the plate is usually placed flat on a work surface, with the left hand holding the reagent and the right hand adding the sample. However, placing the plate horizontally creates a large blind spot, making it difficult for researchers to directly observe the sample wells, increasing the risk of duplicate or missed samples. Furthermore, with the plate horizontally placed, the researcher's sleeves can dangle, increasing the risk of contaminants falling into the plate and contaminating it.

[0003] Secondly, experiments require adding samples to multiple wells within the microplate, with varying locations and areas. Operators typically rely on visual memory, which easily leads to repeated or missed additions to the same well due to memory or counting errors. Colorless, transparent liquids and reagents further complicate memory and counting. Currently, there is no simple and effective device for recording whether samples have been added to the wells. Adding errors not only affect the accuracy of experimental results but also result in a significant waste of manpower, resources, and time.

[0004] Meanwhile, medical research experiments use a wide variety of reagents in different colors, requiring observation, comparison, and recording of the colors of different reactions during operation. Microwell sample addition plates are transparent, and environmental background and lighting significantly affect the observation of the reagents inside. Often, differences in the experimental environment color and the intensity of the top light source being similar to or conflicting with the color of the added reagent can hinder observation, increasing the difficulty for operators to remember, leading to incorrect, excessive, or missed reagent additions, and ultimately causing experimental failure.

[0005] Based on the above problems, a scientific sample loading and cell culture plate operation table needs to be proposed to reduce the difficulty of sample loading and observation and the risk of plate contamination, overcome the influence of background and light on reagent observation, and avoid errors such as over- or under-loading caused by counting errors. Summary of the Invention

[0006] This invention provides a scientific sample loading and cell culture microplate operating table, which can adjust the tilt angle of the loading plate to reduce the difficulty of observation and operational risks during sample loading; it can record the sample loading in a timely and effective manner to avoid duplicate loading and omission errors; and it adds a backlight to adjust the color and brightness of the light source to overcome the influence of experimental background, light and the reagents themselves on observation.

[0007] To achieve the above-mentioned technical objectives and effects, the present invention solves the above-mentioned problems through the following technical solutions:

[0008] A scientific research sample loading and cell culture microplate operating table includes a base, a folding support, and a sample loading plate positioning frame; the two ends of the folding support are respectively hinged to the top of the base and the back of the sample loading plate positioning frame; the top surface of the sample loading plate positioning frame has a plate placement slot for placing and positioning the sample loading plate, and the sample loading plate positioning frame has a built-in background light source to provide background light for the sample loading plate placed in the plate placement slot.

[0009] A sample counting device is hinged to one side of the base. The sample counting device includes at least one counting unit that is hinged sequentially. The counting units can be folded sequentially so that the sample counting device can be stored on the top of the base. Each counting unit includes a counting base plate with multiple rubber protrusions and a reset plate that is slidably connected to the back of the counting base plate by a reset spring. The rubber protrusions are arranged in a matrix on the counting base plate, and the reset plate has hard reset protrusions that correspond to the number and position of the rubber protrusions on the counting base plate.

[0010] In the above scheme, the sample placement frame is mounted on the base via a folding bracket, and the sample is installed in the placement slot opened in the sample placement frame (existing sample placement plates with different numbers of holes have the same external size). The working height and angle of the sample can be adjusted according to the positioning height and angle of the sample placement frame, which can flexibly adapt to the angle requirements of the experimenter. Secondly, the sample placement frame has a built-in background light source to provide background light for the sample, overcoming the influence of background and light on reagent observation.

[0011] Simultaneously, the device is equipped with a sample counting device to record the sample application. The number of rubber protrusions on the sample counting device can be set according to requirements, and its total number of rows and columns is not less than the number of rows and columns of the sample holes in the sample application plate used. After applying the sample to the sample hole, pressing the rubber protrusion on the counting base plate corresponding to the sample hole position can achieve the corresponding counting. After use, by pushing the reset plate upward, the hard reset protrusion will reset all the sample holes on the counting base plate.

[0012] Furthermore, the bottom end of the folding bracket is hinged to the middle of the top surface of the base, the sample counting device is hinged to one side of the base, and a centrifuge tube rack is hinged to the other side; the centrifuge tube rack includes at least one centrifuge tube limiting plate that is hinged in sequence, and the centrifuge tube limiting plate can be folded in sequence to store the centrifuge tube rack on the top of the base; the centrifuge tube limiting plate has multiple centrifuge tube limiting holes; when stored, the sample plate positioning frame is stacked on the sample counting device and the centrifuge tube rack.

[0013] Furthermore, the centrifuge tube limiting plate and the counting substrate are provided with foldable support feet on their side walls, and the support height of each support foot matches the height of the corresponding centrifuge tube limiting plate and the counting substrate when they are laid flat.

[0014] Furthermore, the sample placement frame is stacked on the sample counting device and centrifuge tube rack when stored, and its projection surface is located in the base area; it also includes a protective cover, the bottom of which cooperates with the base, and the sample placement frame, sample counting device, and centrifuge tube rack are covered inside the protective cover when stored.

[0015] Furthermore, the protective cover is equipped with a handle on the top and is locked to the base on both sides by a connecting mechanism; the connecting mechanism consists of positioning pins and hooks respectively located on the protective cover and the base.

[0016] Furthermore, the background light source is a panel LED light with adjustable brightness and color. The side wall of the template positioning frame has an installation groove for the background light source to be placed in. The bottom of the template groove is connected to the installation groove, and the background light source can be detachably installed on the template positioning frame.

[0017] Furthermore, the side wall of the plate placement groove is provided with an arc-shaped loading and unloading hole for easy loading and unloading of the sample plate, and the top surface of the sample plate positioning frame is hinged with a pressure strip to prevent the sample plate from falling off.

[0018] Furthermore, the sample counting device is equipped with two counting units, each of which has 48 arrayed rubber protrusions in a row and column configuration of 6x8. The number and position of the rubber protrusions in the sample counting device composed of the two counting units correspond to the sample holes of the 96-well sample plate.

[0019] The advantages and effects of this invention are:

[0020] 1. The present invention proposes a scientific sample loading and cell culture microplate operating table, wherein the sample loading plate is installed in the plate placement slot opened in the sample loading plate positioning frame. The working height and angle of the sample loading plate can be adjusted according to the positioning height and angle of the sample loading plate positioning frame, which can flexibly adapt to the angle requirements of the experimental personnel for the sample loading plate, reduce the difficulty of sample loading and observation and the risk of well plate contamination.

[0021] 2. The sample placement frame in the solution has a built-in background light source to provide background light for the sample placement. The background light source has brightness and color adjustment functions. By adjusting the background light of the sample placement, the influence of background and light on reagent observation can be overcome.

[0022] 3. The solution includes a sample counting device to record sample additions. After adding sample to the sample hole, pressing the rubber protrusion on the counting base corresponding to the sample hole position enables quick and intuitive counting, avoiding errors such as over- or under-addition due to counting mistakes. After use, pushing the reset plate upwards will quickly reset the sample hole on the counting base using the hard reset protrusion.

[0023] 4. The centrifuge tube rack in the system has the function of holding centrifuge tubes, which can better facilitate the pipetting and sample addition operations and provide convenience for experiments.

[0024] 5. The sample loading and counting device and centrifuge tube rack in the design adopt a modular design, which can be folded and stored on top of the base. Together with the retracted sample loading plate positioning frame, they form a compact storage unit. With the addition of the protective cover and the base, the device can form a portable and mobile scientific sample loading and cell culture microplate operating table. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 First-view unfolded view of the scientific sample loading and cell culture microplate operating table of this invention;

[0027] Figure 3 A second-view unfolded view of the scientific sample loading and cell culture microplate operating table of this invention;

[0028] Figure 4 This is a structural diagram of the storage structure of the scientific sample loading and cell culture microplate operating table of the present invention;

[0029] Figure 5 This is an exploded view of the counting unit.

[0030] Drawing number identifier:

[0031] 1. Base, 2. Folding bracket, 3. Sample positioning frame, 31. Plate slot, 32. Arc-shaped pick-and-place hole, 33. Pressure strip, 4. Background light source, 5. Counting unit, 51. Counting base plate, 511. Rubber protrusion, 512. Support foot, 52. Reset plate, 521. Hard reset protrusion, 53. Reset spring, 6. Centrifuge tube limiting plate, 61. Centrifuge tube limiting hole, 7. Protective cover, 8. Connecting mechanism, 9. Sample loading plate. Detailed Implementation

[0032] The present invention will be further described below with reference to the embodiments, but the present invention is not limited to these embodiments.

[0033] The scientific sample loading and cell culture microplate operating table described in this embodiment is as follows: Figures 1-4 As shown, its main body includes a base 1, a folding bracket 2, a sample placement frame 3, a background light source 4, a sample counting device, a centrifuge tube rack, and a protective cover 7. The bottom end of the folding bracket 2 is hinged to the middle of the top surface of the base 1, and the top end is hinged to the back of the sample placement frame 3. When the folding bracket 2 is folded and stored, the sample placement frame 3 moves closer to the base 1. The interior of the folding bracket 2 and the hinged parts with the base 1 and the sample placement frame 3 use a damping pivot to achieve rotational positioning.

[0034] The top surface of the template positioning frame 3 has a template slot 31 for inserting and positioning the template 9. Since templates with different numbers of holes are of the same size, there is no need to consider the size of the slot 31. The template 9 can be locked in the slot by a pressure strip 33 hinged to the top surface of the template positioning frame 3, preventing the template from falling out. Arc-shaped pick-and-place holes 32 are provided on opposite side walls of the template slot 31 for easy removal. The template can be quickly grasped and placed by inserting a finger into the arc-shaped pick-and-place holes 32.

[0035] The background light source 4 is detachably installed within the sample placement frame 3 to provide background light for the sample placed in the placement slot 31. In this embodiment, the background light source 4 is a panel LED light with adjustable brightness and color. The side wall of the sample placement frame 3 has a mounting slot for the background light source 4 to be placed, and the background light source 4 is detachably installed in the slot. The bottom of the placement slot 31 is connected to the mounting slot, and the light emitted by the background light source 4 can illuminate the sample through the bottom of the placement slot 31. To ensure that the entire surface of the sample receives uniform illumination, the light-emitting surface of the background light source 4 is larger than and covers the bottom surface of the sample 9.

[0036] As attached Figure 2 , 5 As shown, the sample counting device is hinged to the left side of the base. The sample counting device includes multiple counting units 5 that are hinged sequentially. The counting units 5 can be folded sequentially so that the sample counting device can be stored on the top of the base 1. Each counting unit 5 includes a counting base plate 51, a reset plate 52, and a reset spring 53. The edge of the counting base plate 51 is a rigid solid structure, and the middle part is a rubber structure with a matrix of rubber protrusions 511. After the counting unit 5 in the sample counting device is unfolded, the total number of rows and columns of its rubber protrusions 511 is not less than the number of rows and columns of the sample plate to be tested. Taking the upper left corner rubber protrusion 511 and the upper left corner sample hole of the sample plate as the corresponding base points, each rubber protrusion 511 corresponds to a sample hole. When a sample is added to the sample hole, the corresponding rubber protrusion 511 is pressed down.

[0037] In this embodiment, the sample counting device employs two counting units 5. Each counting unit 5 has an array of 48 rubber protrusions 511 arranged in a 6x8 grid. The number and position of the rubber protrusions 511 in the sample counting device composed of the two counting units 5 correspond to the sample holes of the 96-well sample plate. Simultaneously, reference lines A and B are drawn on the counting substrate 51 on the left side after unfolding, facilitating the operator's division of the 12-well and 24-well sample plates. The operator can quickly determine the rubber protrusion 511 area corresponding to the 12-well and 24-well sample plates based on the reference lines. The sample counting device described in this embodiment is applicable to 96-well, 24-well, and 12-well sample plates, and the reference lines can be set according to the required sample plate.

[0038] A reset plate 52 is slidably connected to a groove on the back of the counting substrate 51. A reset spring 53 is provided between the counting substrate 51 and the reset plate 52. The reset plate 52 has hard reset protrusions 521 corresponding to the number and position of the rubber protrusions 511 on the counting substrate 51. During the experiment, after adding a sample to the sample hole on the sample plate, the rubber protrusions 511 on the counting substrate 51 corresponding to the sample hole position can be pressed downwards with a finger to achieve fast, simple, intuitive and effective counting. After the experiment, the reset plate 52 is pressed upwards to overcome the elastic force of the reset spring 53, and the hard reset protrusions 521 push the rubber protrusions 511 upwards to reset them.

[0039] As attached Figure 2 , 3 As shown, the sample counting device is hinged to one side of the base 1, and a centrifuge tube rack is hinged to the other side. The centrifuge tube rack includes at least one centrifuge tube limiting plate 6 that is hinged sequentially. The centrifuge tube limiting plate 6 can be folded sequentially to store the centrifuge tube rack on the top of the base 1. Centrifuge tube limiting holes 61 of different sizes and models can be opened on the centrifuge tube limiting plate 6. In the embodiment, there are two centrifuge tube limiting plates that are hinged sequentially. Each centrifuge tube limiting plate has centrifuge tube limiting holes 61 corresponding to three types of centrifuge tubes, namely one 50ml, two 15ml, and three 1.5ml.

[0040] The centrifuge tube limiting plate 6 and the counting substrate 51 are provided with foldable support feet 512 on their side walls. The support height of each support foot 512 matches the height of the corresponding centrifuge tube limiting plate 6 and the counting substrate 51 when laid flat. In the embodiment, the support feet 512 are arranged symmetrically in pairs, including foot rods hinged to the side walls of the centrifuge tube limiting plate 6 and the counting substrate 51, and connecting rods connecting the foot rods to the side walls of the centrifuge tube limiting plate 6 and the counting substrate 51.

[0041] The cross-sectional dimensions of the sample loading positioning frame 3 are smaller than those of the base 1. When stored, the folding bracket 2 is folded, and the sample loading positioning frame 3 is horizontally stacked on the stored sample loading counting device and centrifuge tube rack. The projection surface of the sample loading positioning frame 3 is located within the area of ​​the base 1. The protective cover 7, which mates with the stepped base 1, can enclose the sample loading positioning frame 3, the sample loading counting device, and the centrifuge tube rack, achieving a protective effect. Simultaneously, the top of the protective cover 7 is equipped with a handle, and both sides are locked to the base 1 via connecting mechanisms 8. The device can form a portable and mobile scientific sample loading and cell culture microplate operating table. In this embodiment, the connecting mechanism 8 consists of positioning pins and hooks respectively located on the protective cover 7 and the base 1, ensuring simple operation and stable connection.

[0042] Using the scientific sample loading and cell culture microplate operating table described in this embodiment, first place the entire unit on the workbench and open the connecting mechanism 8 to remove the protective cover 7. Place the sample loading plate 9 into the plate placement slot 31 of the sample loading plate positioning frame 3 and lock it in place using the pressure strip 33. Adjust the folding bracket 2 to bring the sample loading plate 9 to the target height and angle, and turn on the background light source 4 to adjust it to the required brightness and color.

[0043] When using the sample counting device, unfold each counting unit 5, open the support foot 512, and lay it flat on the side of the base 1. Select the desired working area of ​​the rubber protrusion 511, add the sample to the sample hole on the sample plate, and then press down the rubber protrusion 511 on the counting base plate 51 corresponding to the sample hole position with your finger to achieve fast, simple, intuitive and effective counting.

[0044] When using a centrifuge tube rack, unfold each centrifuge tube limiting plate 6, open the support feet 512, lay it flat on the side of the base 1, and place the centrifuge tubes into the centrifuge tube limiting holes 61 to achieve the limiting position. After using the operating table, reset the rubber protrusions 511 using the reset plate 52, and then store the unfolded parts.

[0045] The microplate operating table for scientific sample loading and cell culture described in this embodiment allows for the rapid installation and adjustment of the sample loading plate in terms of height and angle, and provides the background light required for observation, overcoming the influence of background and light on reagent observation. The sample loading counting device enables timely and effective recording of sample loading, avoiding duplicate loading and omissions, thus facilitating scientific sample loading and cell culture.

[0046] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. For those skilled in the art, various changes, modifications, substitutions, and variations of these embodiments without departing from the principles and spirit of the present invention still fall within the protection scope of the present invention.

Claims

1. A scientific sample loading and cell culture microplate operating table, characterized in that: It includes a base (1), a folding bracket (2), and a sample positioning frame (3); the two ends of the folding bracket (2) are respectively hinged to the top of the base (1) and the back of the sample positioning frame (3); the top surface of the sample positioning frame (3) is provided with a plate slot (31) for placing and positioning the sample; the sample positioning frame (3) has a built-in background light source (4) to provide background light for the sample placed in the plate slot (31); A sample counting device is hinged to one side of the base (1). The sample counting device includes at least one counting unit (5) that is hinged in sequence. The counting unit (5) can be folded in sequence so that the sample counting device can be stored on the top of the base (1). Each counting unit (5) includes a counting base plate (51) with multiple rubber protrusions (511). A reset plate (52) is slidably connected to the back of the counting base plate (51) by a reset spring (53). The rubber protrusions (511) are arranged in a matrix on the counting base plate (51). The reset plate (52) is provided with hard reset protrusions (521) that correspond to the number and position of the rubber protrusions (511) on the counting base plate (51).

2. The scientific sample loading and cell culture microplate work station of claim 1 wherein: The bottom end of the folding bracket (2) is hinged to the middle of the top surface of the base (1), the sample counting device is hinged to one side of the base (1), and the centrifuge tube rack is hinged to the other side; the centrifuge tube rack includes at least one centrifuge tube limiting plate (6) that is hinged in sequence, and the centrifuge tube limiting plate (6) can be folded in sequence so that the centrifuge tube rack is stored on the top of the base (1); the centrifuge tube limiting plate (6) has multiple centrifuge tube limiting holes (61); when stored, the sample plate positioning frame (3) is stacked on the sample counting device and the centrifuge tube rack.

3. The scientific sample loading and cell culture microplate operating table according to claim 2, characterized in that: The centrifuge tube limiting plate (6) and the counting base plate (51) are provided with foldable support feet (512) on their side walls. The support height of each support foot (512) matches the height of the corresponding centrifuge tube limiting plate (6) and the counting base plate (51) when they are laid flat.

4. The scientific sample loading and cell culture microplate operating table according to claim 2, characterized in that: When the sample loading positioning frame (3) is stored, it is stacked on the sample loading counting device and centrifuge tube rack, and its projection surface is located in the area of ​​the base (1); It also includes a protective cover (7), the bottom of which is fitted with the base (1). When storing, a sample positioning frame (3), a sample counting device, and a centrifuge tube rack are placed inside the protective cover (7).

5. The scientific sample loading and cell culture microplate operating table according to claim 4, characterized in that: The protective cover (7) is equipped with a handle on the top and is locked to the base (1) on both sides by a connecting mechanism (8); the connecting mechanism (8) consists of a positioning pin and a hook respectively located on the protective cover (7) and the base (1).

6. The scientific sample loading and cell culture microplate operating table according to claim 1, characterized in that: The background light source (4) is a panel LED light with adjustable brightness and color. The side wall of the template positioning frame (3) is provided with an installation groove for the background light source (4) to be placed. The bottom of the placement groove (31) is connected to the installation groove. The background light source (4) can be detachably installed on the template positioning frame (3).

7. The scientific sample loading and cell culture microplate operating table according to claim 1, characterized in that: The side wall of the plate placement groove (31) is provided with an arc-shaped loading and unloading hole (32) for easy loading and unloading of the sample plate, and the top surface of the sample plate positioning frame (3) is hinged with a pressure strip (33) to prevent the sample plate from falling.

8. The scientific sample loading and cell culture microplate operating table according to claim 1, characterized in that: The sample counting device is provided with two counting units (5), each counting unit (5) is provided with 48 arrayed rubber protrusions (511), and the number of rows and columns is 6X8; the number and position of the rubber protrusions (511) included in the sample counting device composed of the two counting units (5) correspond to the sample holes of the 96-hole sample plate.

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