A compound functional disinfection and bacteriostasis cotton piece used before acne removal and a preparation method thereof

Abstract

The present application relates to the technical field of skin care, and discloses a compound functional disinfection and bacteriostasis cotton sheet used before acne removal and a preparation method, which comprises a substrate layer, the substrate layer is composed of chitosan-hyaluronic acid composite fibers, and the substrate layer has a temperature-responsive release function; a bacteriostasis layer is loaded on the surface or inside of the substrate layer, and the bacteriostasis layer contains thymol with a mass fraction of 0.2% and zinc ion chelate with a mass fraction of 0.5%. The cotton sheet can target and kill acne-causing microorganisms, the bacteriostasis component has no compatibility contraindication with commonly used acne-removing components, and the efficacy of subsequent products is ensured. The intelligent gradient release of the bacteriostasis component is realized by relying on the temperature responsiveness of the substrate, the utilization rate of the components is improved, and the problem of easy loss of effective components is solved. The skin microenvironment can be adjusted to be a physiological weak acid, the alcohol system is abandoned, and a repair layer is matched, so that the skin barrier is repaired gently and non-stimulatingly, and the four mechanisms crack the technical problem that the bacteriostasis effect and skin tolerance are difficult to balance.

Description

Technical Field

[0001] This invention relates to the field of skin care technology, specifically to a multifunctional disinfectant and antibacterial cotton pad for use before acne treatment and its preparation method. Background Technology

[0002] Acne (commonly known as pimples) is a common chronic inflammatory disease of the hair follicles and sebaceous glands. Its pathogenesis is complex, mainly related to abnormal keratinization of the hair follicle opening, excessive sebum secretion, proliferation of microorganisms such as Propionibacterium acnes, and inflammatory responses. In clinical treatment, cleansing and pre-treating the skin before using acne treatment products is of great significance for unclogging hair follicles, removing sebum and keratin debris, inhibiting pathogenic microorganisms, promoting the penetration of subsequent products, and improving efficacy.

[0003] Existing acne pretreatment products have the following technical shortcomings:

[0004] Traditional disinfectant products mostly use a single alcohol system, achieving instant sterilization through rapid evaporation. However, while high-concentration alcohol removes sebum, it also excessively removes moisture from the stratum corneum, damaging the skin barrier function. This, in turn, stimulates the sebaceous glands to compensate by secreting more oil, creating a vicious cycle of "the more you wash, the oilier it gets." This not only fails to help control acne but may even worsen existing acne.

[0005] There are incompatibilities between the antibacterial ingredients in existing products and the core ingredients for subsequent acne treatment. For example, certain cationic surfactants or antibiotics are prone to neutralization, precipitation, or redox reactions when in contact with commonly used acne-fighting active ingredients such as salicylic acid and benzoyl peroxide, leading to the inactivation or degradation of the active ingredients and seriously affecting the efficacy of subsequent treatment products.

[0006] The technology for loading and releasing active ingredients onto cotton pad carriers is still imperfect. Existing products generally achieve an encapsulation rate of less than 65% for active ingredients, leading to degradation or adsorption of these ingredients onto the carrier fibers during storage, resulting in loss. This not only wastes active raw materials but also makes it difficult to ensure sufficient and uniform application of active ingredients to the target area during use, affecting the actual effectiveness of pretreatment. Therefore, we propose a multifunctional disinfectant and antibacterial cotton pad for use before acne treatment and its preparation method to address the aforementioned problems. Summary of the Invention

[0007] To address the shortcomings of existing technologies, this invention provides a multifunctional disinfectant and antibacterial cotton pad for use before acne treatment, which solves the problem that traditional acne pretreatment products with a single alcohol system easily damage the skin barrier and induce compensatory sebum secretion.

[0008] To achieve the above objectives, the present invention provides the following technical solution: a composite functional disinfectant and antibacterial cotton pad for use before acne treatment and a method for its preparation, comprising:

[0009] The substrate layer is composed of chitosan-hyaluronic acid composite fibers and has a temperature-responsive release function.

[0010] An antibacterial layer is loaded on the surface or inside the substrate layer, the antibacterial layer comprising 0.2% thymol by mass and 0.5% zinc ion chelate by mass.

[0011] A buffer layer, dispersed in the substrate layer, the buffer layer comprising polymethyl methacrylate microspheres encapsulated with citric acid / sodium citrate buffer solution;

[0012] A repair layer is disposed on the outer surface of the substrate layer, the repair layer comprising ceramide NP inclusions.

[0013] Preferably, the mass ratio of chitosan to hyaluronic acid in the chitosan-hyaluronic acid composite fiber is 7:3, the molecular weight of the chitosan is 100,000 Daltons, and the degree of deacetylation is 85%.

[0014] Preferably, the zinc ion chelate is a chelate of Zn²⁺ and EDTA, wherein the molar ratio of zinc ions to EDTA in the antibacterial layer is 1:1.5-2.5.

[0015] Preferably, the mass fraction of the citric acid / sodium citrate buffer in the buffer layer is 5%, and the particle size of the polymethyl methacrylate microspheres is 30-150 μm.

[0016] Preferably, the mass fraction of ceramide NP inclusions in the repair layer is 1%, and the ceramide NP inclusions are prepared by phase inversion.

[0017] Preferably, the chitosan-hyaluronic acid composite fiber is temperature responsive, with a release rate of 20% for the antibacterial component at 25°C and 85% at 37°C.

[0018] Preferably, the polymethyl methacrylate microspheres in the buffer layer rupture upon contact with the skin, releasing a citrate buffer solution to maintain the skin surface pH at 5.5-6.0.

[0019] This invention provides a method for preparing a composite functional disinfectant and antibacterial cotton pad for use before acne treatment, comprising the composite functional disinfectant and antibacterial cotton pad for use before acne treatment as described in any one of claims 1-7, characterized by comprising the following steps:

[0020] S1. Chitosan and hyaluronic acid are dissolved in 1% acetic acid solution at a mass ratio of 7:3, and chitosan-hyaluronic acid composite fiber is prepared by wet spinning process.

[0021] S2. Using electrospinning technology, polymethyl methacrylate microspheres coated with citric acid / sodium citrate buffer solution are combined with spinning solution to form a nanofiber membrane.

[0022] S3. The composite fiber prepared in S1 is impregnated in a solution containing 0.2% thymol and 0.5% zinc ion chelate by impregnation method for 30 minutes at a temperature of 40°C, so that the antibacterial components are loaded onto the fiber surface.

[0023] S4. The nanofiber membrane prepared in S2 is combined with the composite fiber loaded with the antibacterial layer in S3, and a 1% ceramide NP encapsulation prepared by phase inversion method is coated on the outer surface of the composite fiber to form a repair layer.

[0024] S5. Electron beam sterilization is used with a sterilization dose of 15 kGy, resulting in a microbial survival rate of less than 0.01%.

[0025] Preferably, in step S2, the diameter of the nanofiber membrane is 200 nm.

[0026] Preferably, in step S2, the polymethyl methacrylate microspheres have a particle size of 30-150 μm and the encapsulated citric acid / sodium citrate buffer has a mass fraction of 5%.

[0027] Beneficial effects

[0028] This invention provides a multifunctional disinfectant and antibacterial cotton pad for use before acne treatment and its preparation method. Compared with the prior art, it has the following beneficial effects:

[0029] This multifunctional disinfectant and antibacterial cotton pad, used before acne treatment, and its preparation method, utilize thymol and zinc ion-EDTA chelate to form an antibacterial layer. This layer can target and destroy the biofilm of Propionibacterium acnes for highly effective disinfection. Furthermore, this antibacterial component has no incompatibility with commonly used acne-treating ingredients such as salicylic acid and benzoyl peroxide, and will not undergo neutralization or degradation reactions, effectively ensuring the efficacy of subsequent acne-treating products. Simultaneously, leveraging the temperature-responsive characteristics of chitosan-hyaluronic acid composite fibers, it achieves intelligent gradient release of the antibacterial component, reducing component degradation loss during storage and ensuring sufficient component application to the target area upon skin contact, significantly improving component utilization and solving the problems of low encapsulation rate and easy loss of effective ingredients in existing products.

[0030] It also achieves a synergistic effect of skin microenvironment regulation and barrier repair, while significantly improving the overall effect of pre-treatment before acne treatment. When the polymethyl methacrylate microspheres in the buffer layer come into contact with the skin, they rupture, releasing a citric acid / sodium citrate buffer solution. This maintains the skin's surface pH within a physiologically appropriate, slightly acidic range, protecting the skin's own defense capabilities and preventing the re-proliferation of acne-causing microorganisms. This invention abandons the traditional single alcohol disinfection system, combining it with a repair layer containing ceramide NP encapsulations, significantly reducing damage to the stratum corneum and effectively repairing the skin barrier while being gentle and non-irritating. Through a four-pronged mechanism of targeted elimination, dynamic pH adjustment, intelligent ingredient release, and barrier repair, it achieves comprehensive pre-treatment of the skin before acne treatment, successfully solving the technical challenge of balancing the antibacterial effect and skin tolerance in existing pre-treatment products. Detailed Implementation

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] A multifunctional disinfectant and antibacterial cotton pad for use before acne treatment includes a base layer, an antibacterial layer, a buffer layer, and a repair layer. These functional layers work synergistically to achieve multiple pretreatment effects.

[0033] Substrate layer: Composed of chitosan-hyaluronic acid composite fibers, with a mass ratio of chitosan to hyaluronic acid of 7:3. The molecular weight of chitosan is 100,000 Daltons, and the degree of deacetylation is 85%. The substrate layer has a temperature-responsive release function, with a release rate of 20% for antibacterial components at 25℃ and 85% at 37℃, enabling gradient intelligent release of antibacterial components.

[0034] Antibacterial layer: Loaded on the surface or inside of the substrate layer, the antibacterial layer contains 0.2% by mass of thymol and 0.5% by mass of zinc ion chelate; the zinc ion chelate is a chelate of Zn²⁺ and EDTA, wherein the molar ratio of zinc ions to EDTA is 1:1.5-2.5, which can target and destroy the biofilm structure of Propionibacterium acnes to achieve highly efficient antibacterial effect;

[0035] Buffer layer: Dispersed in the substrate layer, the buffer layer contains polymethyl methacrylate microspheres encapsulated with citric acid / sodium citrate buffer; the mass fraction of citric acid / sodium citrate buffer is 5%, and the particle size of polymethyl methacrylate microspheres is 30-150μm. The microspheres rupture upon contact with the skin, releasing the citric acid buffer, so that the pH value of the skin surface is maintained in the weakly acidic physiological range of 5.5-6.0.

[0036] Repair layer: Located on the outer surface of the substrate layer, the repair layer contains 1% by mass of ceramide NP inclusions. The ceramide NP inclusions are prepared by phase inversion method, which can repair the skin barrier and reduce skin irritation while inhibiting bacteria and disinfecting.

[0037] This invention provides a method for preparing a multifunctional disinfectant and antibacterial cotton pad for use before acne treatment, comprising the following steps:

[0038] S1. Chitosan and hyaluronic acid are dissolved in 1% acetic acid solution at a mass ratio of 7:3, and chitosan-hyaluronic acid composite fiber is prepared by wet spinning process to serve as the substrate layer of cotton sheet.

[0039] S2. Using electrospinning technology, polymethyl methacrylate microspheres coated with citric acid / sodium citrate buffer are combined with spinning solution to form a nanofiber membrane with a fiber diameter of 200 nm; wherein the particle size of polymethyl methacrylate microspheres is 30-150 μm, and the mass fraction of citric acid / sodium citrate buffer is 5%;

[0040] S3. The composite fiber prepared in S1 is impregnated in a solution containing 0.2% thymol and 0.5% zinc ion chelate by impregnation method for 30 minutes at a temperature of 40°C, so that the antibacterial components are fully loaded on the surface and inside of the fiber to form an antibacterial layer.

[0041] S4. The nanofiber membrane prepared in S2 is combined with the composite fiber loaded with the antibacterial layer in S3, so that the buffer layer is dispersed in the substrate layer; and 1% ceramide NP inclusions prepared by phase inversion method are coated on the outer surface of the composite fiber to form a repair layer, thus obtaining the initial cotton pad product.

[0042] S5. The initial cotton pads are sterilized using electron beam sterilization at a dose of 15 kGy, resulting in a microbial survival rate of less than 0.01% on the cotton pads, thus obtaining the finished disinfectant and antibacterial cotton pads.

[0043] Example 1;

[0044] Chitosan-hyaluronic acid composite fiber substrate, with a chitosan to hyaluronic acid mass ratio of 7:3, chitosan molecular weight of 100,000 Daltons, and degree of deacetylation of 85%; antibacterial solution containing 0.2% thymol and 0.5% zinc ion chelate, with a zinc ion to EDTA molar ratio of 1:2; polymethyl methacrylate (PMMA) microspheres (particle size 50-100 μm) encapsulated with 5% citric acid / sodium citrate buffer; and 1% ceramide NP inclusions (prepared by phase inversion method).

[0045] Chitosan-hyaluronic acid fiber substrate with a size of 15cm×15cm was cut and placed in a plasma treatment device with a power of 200W and a treatment time of 2 minutes to activate the surface of the substrate and enhance the surface activity of the substrate and the loading and binding ability of subsequent antibacterial components.

[0046] The plasma-activated fiber substrate was impregnated in an antibacterial solution containing 0.2% thymol and 0.5% zinc ion chelate. The impregnation temperature was controlled at 40°C and the impregnation time was 30 minutes, so that the antibacterial components were fully and evenly loaded on the surface and inside of the substrate to form an antibacterial layer.

[0047] A nanofiber membrane (fiber diameter 200nm) prepared by PMMA microspheres coated with 5% citric acid / sodium citrate buffer was hot-pressed with a fiber substrate loaded with an antibacterial layer. The hot-pressing pressure was set to 0.3MPa and the hot-pressing temperature was 50℃, so that the buffer layer was uniformly dispersed in the substrate layer, and the composite molding of the substrate layer, antibacterial layer and buffer layer was completed.

[0048] After composite molding, a 1% ceramide NP inclusion body prepared by phase inversion method is uniformly coated on the outer surface of the fiber to form a repair layer, thus obtaining the crude cotton sheet.

[0049] The crude cotton pads are sterilized by electron beam with a sterilization dose of 15 kGy to ensure that the survival rate of microorganisms on the cotton pads is less than 0.01%. After sterilization, the cotton pads are cut into 5cm×5cm square pieces to obtain the standard pre-acne treatment multi-functional disinfection and antibacterial cotton pads.

[0050] Example 2:

[0051] Chitosan-hyaluronic acid composite fiber substrate (parameters same as in Example 1); antibacterial solution containing 0.2% thymol and 0.5% zinc ion chelate (parameters same as in Example 1); PMMA microspheres encapsulated with 5% citric acid / sodium citrate buffer (parameters same as in Example 1); 1% ceramide NP inclusions (prepared by phase inversion method); 0.1% tea tree oil microcapsules; special solvent and auxiliaries for supercritical CO2 extraction;

[0052] The plasma activation treatment of a 15cm×15cm chitosan-hyaluronic acid fiber substrate was completed according to the parameters of step 1 in Example 1.

[0053] A nanoscale active ingredient dispersion system was prepared using supercritical CO2 extraction technology. The extraction pressure was set at 30 MPa and the extraction temperature at 40 °C. 0.1% tea tree oil microcapsules were mixed with an antibacterial solution containing 0.2% thymol and 0.5% zinc ion chelate to obtain a composite antibacterial dispersion.

[0054] The plasma-activated fiber substrate is immersed in the above-mentioned composite antibacterial dispersion, with the immersion temperature controlled at 40°C and the immersion time at 30 minutes, so that the composite antibacterial components are loaded onto the surface and interior of the substrate to form a reinforced antibacterial layer.

[0055] According to the hot pressing parameters in step 3 of Example 1, the PMMA microsphere nanofiber membrane and the fiber substrate with the reinforced antibacterial layer are hot-pressed together to achieve the combination of the buffer layer, the substrate layer, and the reinforced antibacterial layer.

[0056] 1% ceramide NP encapsulation was mixed with the remaining nano-sized tea tree oil microcapsule dispersion and uniformly coated on the outer surface of the composite-molded fiber to form a reinforced repair layer, thus obtaining the crude cotton pad product.

[0057] Following the sterilization parameters in step 5 of Example 1, the crude cotton pads were subjected to electron beam sterilization. After sterilization, they were cut into 5cm×5cm square cotton pads to obtain the finished product of the enhanced pre-acne treatment multi-functional disinfectant and antibacterial cotton pads.

[0058] Performance testing was conducted on the cotton pads prepared in Examples 1 and 2 above, with a control group consisting of traditional alcohol-based acne-treating cotton pads. The test results are as follows:

[0059] Antibacterial properties: The minimum inhibitory concentration (MIC) of the cotton pad in Example 1 against Propionibacterium acnes was 0.015 mg / mL, which was 83% lower than that of traditional products, and the antibacterial rate was ≥99.5%; The cotton pad in Example 2, due to the addition of tea tree oil microcapsules, had a MIC reduced to 0.012 mg / mL and an antibacterial rate increased to over 99.8%.

[0060] Skin tolerance: 50 volunteers with acne-prone skin were selected for trial testing. After using the cotton pads in Example 1, the transepidermal water loss (TEWL) value of the volunteers' skin was reduced by 41.7% compared with the control group. The TEWL value of the cotton pads in Example 2 was reduced by 45.2% compared with the control group. Neither cotton pad showed obvious skin irritation, and the incidence of irritation was much lower than that of traditional products.

[0061] Ingredient stability: The two cotton pads were placed in an accelerated testing environment of 40℃ / 75%RH. After 30 days, the retention rate of active ingredients was tested. The retention rate of active ingredients in the cotton pad of Example 1 was 94.3%, and the retention rate of active ingredients in the cotton pad of Example 2 was 92.7%, both of which are far higher than the average encapsulation / retention rate of 65% of existing products.

[0062] This solution utilizes a thymol-zinc ion-EDTA chelate to form an antibacterial layer, which can target and destroy the biofilm of Propionibacterium acnes for highly effective elimination. Furthermore, this antibacterial component has no incompatibilities with commonly used acne-fighting ingredients such as salicylic acid and benzoyl peroxide, and will not undergo neutralization or degradation reactions, effectively ensuring the efficacy of subsequent acne-fighting products. Simultaneously, leveraging the temperature-responsive characteristics of chitosan-hyaluronic acid composite fibers, it achieves intelligent gradient release of the antibacterial component, reducing degradation loss during storage and ensuring sufficient component application to the target area upon skin contact, significantly improving component utilization and solving the problems of low encapsulation rate and easy loss of effective ingredients in existing products.

[0063] It also achieves a synergistic effect of skin microenvironment regulation and barrier repair, while significantly improving the overall effect of pre-treatment before acne treatment. When the polymethyl methacrylate microspheres in the buffer layer come into contact with the skin, they rupture, releasing a citric acid / sodium citrate buffer solution. This maintains the skin's surface pH within a physiologically appropriate, slightly acidic range, protecting the skin's own defense capabilities and preventing the re-proliferation of acne-causing microorganisms. This invention abandons the traditional single alcohol disinfection system, combining it with a repair layer containing ceramide NP encapsulations, significantly reducing damage to the stratum corneum and effectively repairing the skin barrier while being gentle and non-irritating. Through a four-pronged mechanism of targeted elimination, dynamic pH adjustment, intelligent ingredient release, and barrier repair, it achieves comprehensive pre-treatment of the skin before acne treatment, successfully solving the technical challenge of balancing the antibacterial effect and skin tolerance in existing pre-treatment products.

[0064] It should be noted that: all contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0065] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A compound functional disinfection and bacteriostasis cotton sheet for acne treatment before use and a preparation method, characterized in that, include: The substrate layer is composed of chitosan-hyaluronic acid composite fibers and has a temperature-responsive release function. An antibacterial layer is loaded on the surface or inside the substrate layer, the antibacterial layer comprising 0.2% thymol by mass and 0.5% zinc ion chelate by mass. A buffer layer, dispersed in the substrate layer, the buffer layer comprising polymethyl methacrylate microspheres encapsulated with citric acid / sodium citrate buffer solution; A repair layer is disposed on the outer surface of the substrate layer, the repair layer comprising ceramide NP inclusions.

2. The composite functional disinfectant and antibacterial cotton pad for use before acne treatment according to claim 1, characterized in that: The chitosan-hyaluronic acid composite fiber has a chitosan to hyaluronic acid mass ratio of 7:3, the chitosan has a molecular weight of 100,000 Daltons and a degree of deacetylation of 85%.

3. The composite functional disinfectant and antibacterial cotton pad for use before acne treatment according to claim 1, characterized in that: The zinc ion chelate is a chelate of Zn²⁺ and EDTA, wherein the molar ratio of zinc ions to EDTA in the antibacterial layer is 1:1.5-2.

5.

4. The composite functional disinfection and bacteriostasis cotton sheet for acne treatment before use according to claim 1, characterized in that: The buffer layer contains 5% citric acid / sodium citrate buffer by mass, and the polymethyl methacrylate microspheres have a particle size of 30-150 μm.

5. The composite functional disinfectant and antibacterial cotton pad for use before acne treatment according to claim 1, characterized in that: The mass fraction of ceramide NP inclusions in the repair layer is 1%, and the ceramide NP inclusions are prepared by phase inversion method.

6. The composite functional disinfection and bacteriostasis cotton sheet for acne treatment before use according to claim 1, characterized in that: The chitosan-hyaluronic acid composite fiber is temperature responsive, with a release rate of 20% for the antibacterial component at 25°C and 85% at 37°C.

7. The composite functional disinfection and bacteriostasis cotton sheet for acne treatment before use according to claim 1, characterized in that: The polymethyl methacrylate microspheres in the buffer layer rupture upon contact with the skin, releasing a citrate buffer solution to maintain the skin surface pH at 5.5-6.

0.

8. A method for preparing the composite functional disinfection and bacteriostasis cotton sheet for use before acne treatment, comprising the composite functional disinfection and bacteriostasis cotton sheet for use before acne treatment according to any one of claims 1-7, characterized in that, Includes the following steps: S1. Chitosan and hyaluronic acid are dissolved in 1% acetic acid solution at a mass ratio of 7:3, and chitosan-hyaluronic acid composite fiber is prepared by wet spinning process. S2. Using electrospinning technology, polymethyl methacrylate microspheres coated with citric acid / sodium citrate buffer solution are combined with spinning solution to form a nanofiber membrane. S3. The composite fiber prepared in S1 is impregnated in a solution containing 0.2% thymol and 0.5% zinc ion chelate by impregnation method for 30 minutes at a temperature of 40°C, so that the antibacterial components are loaded onto the fiber surface. S4. The nanofiber membrane prepared in S2 is combined with the composite fiber loaded with the antibacterial layer in S3, and a 1% ceramide NP encapsulation prepared by phase inversion method is coated on the outer surface of the composite fiber to form a repair layer. S5. Electron beam sterilization is used with a sterilization dose of 15 kGy, resulting in a microbial survival rate of less than 0.01%.

9. The method for preparing the multifunctional disinfection and sterilization cotton sheet for acne treatment before use according to claim 8, characterized in that: In step S2, the diameter of the nanofiber membrane is 200 nm.

10. The method for preparing the multifunctional disinfection and sterilization cotton sheet for acne treatment before use according to claim 8, characterized in that: In step S2, the polymethyl methacrylate microspheres have a particle size of 30-150 μm and the encapsulated citric acid / sodium citrate buffer has a mass fraction of 5%.