Probe as well as preparation method and application thereof

By designing a time-responsive dual-lock activation probe, utilizing the time-response of MMP-specific peptides and pH-sensitive polymer layers, the false-positive problem of boundary identification in tumor surgery by existing probes is solved, achieving highly specific identification and accurate resection of the tumor invasion front.

CN122321180APending Publication Date: 2026-07-03PEOPLES HOSPITAL OF ZHENGZHOU
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Patent Information

Application Number
CN202610629840.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-09
Publication Date
2026-07-03

AI Technical Summary

Technical Problem

Existing probes have a high false positive rate in tumor surgery and cannot accurately identify tumor boundaries, especially the distinction between the invasion front and the inflammatory and necrotic areas, leading to inaccurate resection range.

Method used

A time-responsive dual-lock activation probe was designed, comprising a near-infrared II fluorescent core, a gold nanoshell, an MMP-specific peptide, and a pH-sensitive polymer layer. Fluorescence recovery was achieved through time-series events of enzymatic cleavage and acid response, ensuring specific activation at the forefront of tumor invasion.

Benefits of technology

It achieves highly specific identification of the tumor invasion front, reduces the false positive rate, can identify tiny invasive lesions with a diameter ≤1mm, has an imaging depth ≥5mm, and has a response speed suitable for use during surgery.

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Abstract

The invention relates to the field of biological nanotechnology and molecular imaging, and discloses a probe as well as a preparation method and application thereof. The probe sequentially comprises a near-infrared second-region (NIR-II) fluorescent core, a gold nano shell layer, an MMP response peptide fragment layer and a pH sensitive polymer layer from inside to outside. The probe is in a silent state in a circulating system by utilizing the quenching effect of the gold shell layer on core fluorescence. The core design of the probe is that through the physical shielding effect of the peptide fragment layer, it is ensured that the probe must be cut and unlocked through tumor high-expression MMP enzyme firstly, and then the polymer layer is triggered in a slightly acidic environment to be protonated and fall off, so that fluorescence can be recovered. According to the invention, the non-specific activation of a single response probe in an inflammation or necrosis region is effectively overcome, the imaging contrast ratio of the tumor invasion leading edge is remarkably improved, and a high-sensitivity and deep-penetration visual navigation means is provided for precise tumor surgery excision.
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