Long-acting degerming and deodorizing spray, its preparation method and application in pet cat litter

Abstract

The application discloses a long-acting bacteria-removing and odor-removing spray, a preparation method thereof and application of the spray in pet cat litter, and the spray comprises the following raw materials in parts by weight: 0.5-3.0 parts of trihydroxybutyric acid oligomer (OPHB); 1-1.5 parts of a composite biological enzyme preparation; 0.1-0.15 parts of a plant essential oil; 0.1-0.5 parts of sodium bicarbonate; 0.1-0.2 parts of a surfactant; and 90-100 parts of deionized water; and the composite biological enzyme preparation comprises urease, protease, lipase and amylase. The spray is a multifunctional spray preparation with the trihydroxybutyric acid oligomer (OPHB) as an antibacterial effective component, the composite biological enzyme preparation as a deodorization core component, and a small amount of the plant essential oil, the sodium bicarbonate and the surfactant as auxiliary components, and is suitable for daily cleaning and odor control of cat litter in scenes such as families, pet stores and pet hospitals.

Description

Technical Field

[0001] This invention belongs to the technical field of pet antibacterial and deodorizing products, specifically relating to a long-lasting antibacterial and deodorizing spray, its preparation method, and its application in pet cat litter. Background Technology

[0002] With the rapid development of the pet economy and the increasing popularity of pet cat ownership, cat litter, as a core product for cat excretion management, has become a key concern for cat-owning families in terms of hygiene and odor control. Existing cat litter deodorizing products often rely on fragrances to mask odors or use chemical bactericides (such as quaternary ammonium salts and hypochlorous acid), which can cause problems such as strong irritation, residual toxicity, and respiratory sensitivities in cats. Furthermore, traditional deodorizing products have limited ability to decompose typical malodorous gases in cat litter, such as ammonia and hydrogen sulfide, and long-term use can disrupt the microecological balance of the cat litter. Summary of the Invention

[0003] Purpose of the invention: Addressing the problems of incomplete odor removal, short-lasting sterilization, and strong irritation in existing cat litter cleaning technologies, this invention proposes a long-lasting antibacterial and odor-removing spray, its preparation method, and its application in pet cat litter. Specifically, it involves a multifunctional spray formulation with oligo(hydroxybutyric acid) oligomer (OPHB) as the antibacterial active ingredient, a compound biological enzyme preparation as the core odor-removing ingredient, and supplemented with small amounts of plant essential oils, sodium bicarbonate (baking soda), and surfactants. This formulation is suitable for daily cleaning and odor control of cat litter in homes, pet stores, and veterinary clinics.

[0004] Technical Solution: To achieve the above-mentioned objectives, the technical solution adopted by this invention is as follows:

[0005] In a first aspect, the present invention provides a long-lasting antibacterial and deodorizing spray, the spray comprising the following raw materials in parts by weight:

[0006] Trihydroxybutyric acid oligomer (OPHB): 0.5–3.0 parts;

[0007] Compound biological enzyme preparation: 1-1.5 parts;

[0008] Plant essential oil: 0.1–0.15 parts;

[0009] Sodium bicarbonate: 0.1–0.5 parts;

[0010] Surfactant: 0.1-0.2 parts;

[0011] Deionized water: 90-100 parts;

[0012] The compound bio-enzyme preparation includes urease, protease, lipase and amylase.

[0013] Preferably, in the compound biological enzyme preparation, the mass ratio of urease, protease, lipase and amylase is (40-60):(20-40):(5-15):(5-10).

[0014] Preferably, the spray comprises the following raw materials in parts by weight:

[0015] Trihydroxybutyric acid oligomer (OPHB): 1-1.5 parts;

[0016] Compound biological enzyme preparation: 1-1.5 parts;

[0017] Plant essential oil: 0.1–0.15 parts;

[0018] Sodium bicarbonate: 0.3–0.4 parts;

[0019] Surfactant: 0.1-0.2 parts;

[0020] Deionized water: 90-100 parts.

[0021] Preferably, the plant essential oil is selected from one or more of green tea essential oil, white tea essential oil, jasmine essential oil, and lavender essential oil.

[0022] Preferably, the surfactant is one or more of lauryl phosphate and aminomethyl propanol, which are widely used in the cosmetics industry and infant care products. They are mild, non-irritating, and safe.

[0023] Secondly, the present invention provides a method for preparing the aforementioned long-lasting antibacterial and deodorizing spray, comprising the following steps:

[0024] 1) Dissolve OPHB in deionized water, slowly add the surfactant, and stir at low speed until evenly dispersed;

[0025] 2) Add sodium bicarbonate and continue stirring until completely dissolved, then adjust the pH to 6.5-7.2;

[0026] 3) Add compound biological enzyme preparation and plant essential oil at a temperature below 30°C and stir to obtain a mixture;

[0027] 4) Filter the above mixture and fill it into the spraying device to obtain the finished product.

[0028] As a specific implementation plan, in step 1), the low-speed stirring is to stir at 20-30°C for 10-20 minutes.

[0029] As a specific implementation plan, in step 3), the stirring time is 10-20 minutes.

[0030] Thirdly, the present invention provides the application of the long-lasting antibacterial and deodorizing spray in the antibacterial and deodorizing of pet cat litter.

[0031] As a specific implementation plan, the amount of the spray is 10 to 20 mL per liter of pet cat litter.

[0032] The spray of the present invention can be applied using conventional spraying devices, such as manually operated press bottles (without propellant), with the nozzle equipped with a microporous atomizing nozzle (particle size 50-150μm).

[0033] In specific applications, this includes:

[0034] Routine maintenance: Spray 1-2 times daily, using 5-10 mL / m². 2 (Approximately 10-20 mL of cat litter per liter)

[0035] Immediate treatment after excretion: After cleaning up feces or urine, focus on spraying the contaminated area;

[0036] Spraying distance: 20-30cm from the surface of the cat litter, evenly cover, and let it air dry after spraying (no additional treatment required);

[0037] Suitable for: multi-cat households, pet stores, animal hospitals, etc.

[0038] In this invention, trihydroxybutyrate oligomer (OPHB), as a low molecular weight derivative of polyhydroxybutyrate (PHB), exhibits excellent biocompatibility and broad-spectrum antibacterial activity, particularly showing significant inhibitory effects against Gram-negative bacteria, Gram-positive bacteria, and common environmental fungi, with no toxic side effects on mammals. Simultaneously, the compound bio-enzyme preparation can catalytically decompose organic matter such as urea, protein, and fat in urine, achieving a long-lasting antibacterial effect that reduces odor generation at its source. Combining the natural fragrance of plant essential oils with the physical adsorption properties of baking soda, a synergistic effect of "antibacterial—odor removal—fragrance enhancement" can be achieved.

[0039] The specific mechanism of action is as follows:

[0040] 1. Triple antibacterial mechanism:

[0041] Cell membrane disruption: OPHB inserts into the bacterial cell membrane through hydrophobic interactions, causing the membrane structure to rupture and contents to leak out;

[0042] Metabolic interference: Active ingredients in plant essential oils, such as tea polyphenols (EGCG, flavonoids), inhibit bacterial DNA synthesis and enzyme activity;

[0043] 2. Three stages of deodorization:

[0044] Source decomposition: Enzyme preparations hydrolyze urea (urease), protein (protease), and fat (lipase), reducing the production of ammonia and amines;

[0045] Chemical neutralization: The active ingredients in plant essential oils, such as tea polyphenols, are converted into harmless small molecules through hydrogen bonding or redox reactions with thiols and amines.

[0046] Long-lasting inhibition: OPHB continuously inhibits the growth and metabolism of residual bacteria, preventing the regeneration of odors.

[0047] 3. Safety and environmental protection guarantees:

[0048] OPHB is a product of microbial fermentation and is completely biodegradable; all kinds of enzyme preparations and plant essential oils are of natural origin and are non-toxic and harmless.

[0049] Beneficial effects: Compared with the prior art, the present invention has the following advantages:

[0050] 1. Highly effective sterilization: OPHB achieves long-term inhibition of common bacteria (such as Escherichia coli and Staphylococcus aureus) and fungi in cat litter by interfering with the structure of microbial cell membranes and inhibiting biofilm formation, with an antibacterial rate of ≥99% (24 hours).

[0051] 2. Odor removal at the source: The compound biological enzyme preparation can quickly decompose urea in urine to generate ammonia precursors, proteins and fats as odor sources, significantly reducing the concentration of malodorous gases such as ammonia and hydrogen sulfide, with an odor removal rate of ≥90%.

[0052] 3. Safe and non-irritating: All ingredients are biodegradable, non-toxic, and residue-free. They are non-irritating to the skin and respiratory tract of cats and are suitable for kittens, senior cats, and cats with sensitive constitutions.

[0053] 4. Synergistic effect: Baking soda absorbs residual odors, plant essential oils provide a fresh and natural fragrance, and surfactants promote the spread of droplets on the cat litter surface, improving the contact efficiency of active ingredients.

[0054] 5. Easy to use: The spray design allows for precise control of the dosage. Spraying the cat litter surface 1-2 times a day can maintain a clean environment for more than 7 days. Attached Figure Description

[0055] Figure 1 This is a schematic diagram of an apparatus for the digestion of ammonia in a sealed environment. Detailed Implementation

[0056] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0057] The trihydroxybutyric acid oligomers in the following examples were all prepared according to the method in Example 1-1 of Chinese Patent CN116178159A, as detailed below:

[0058] In a three-necked flask, 10 g of ethyl 3-hydroxybutyrate and 0.01 g of zinc acetate (equivalent to 0.1% of the mass of ethyl 3-hydroxybutyrate) were added, accompanied by mechanical stirring, a thermometer, and a distillation apparatus. The stirring speed was slow at about 150 rpm, and the temperature was increased to 150 °C at 0.5-3 °C / min under a trace amount of nitrogen atmosphere for 1 hour. The reaction was then stopped to obtain 3-HB oligomer, which is the trihydroxybutyrate oligomer (OPHB).

[0059] Example 1: Preparation of a long-lasting antibacterial and deodorizing spray

[0060] formula:

[0061] Trihydroxybutyrate oligomer (OPHB): 1.3 parts;

[0062] Compound biological enzyme preparation: 1.2 parts;

[0063] Green tea essential oil: 0.1 parts;

[0064] Sodium bicarbonate: 0.3 parts;

[0065] Lauryl phosphate: 0.1 parts;

[0066] Deionized water: 97 parts.

[0067] The compound biological enzyme preparation includes urease, protease, lipase and amylase in a mass ratio of 50:30:10:8.

[0068] Preparation method:

[0069] 1) Dissolve OPHB in deionized water, slowly add surfactant, stir at low speed to ensure that no excessive foaming occurs, and stir at 25°C for 15 minutes until evenly dispersed;

[0070] 2) Add baking soda and continue stirring until completely dissolved, then adjust the pH to 6.8;

[0071] 3) Add the compound biological enzyme preparation and plant essential oil at a temperature below 30°C, and stir for 15 minutes;

[0072] 4) Filter the above mixture, fill it into the spraying device, and label it to obtain the finished product.

[0073] The spraying device uses a manually press-down bottle (without propellant), and the nozzle is equipped with a microporous atomizing nozzle (particle size 50-150μm).

[0074] Example 2: Preparation of a long-lasting antibacterial and deodorizing spray

[0075] formula:

[0076] Trihydroxybutyrate oligomer (OPHB): 1 part;

[0077] Compound biological enzyme preparation: 1 part;

[0078] White tea essential oil: 0.1 parts;

[0079] Sodium bicarbonate: 0.3 parts;

[0080] Lauryl phosphate: 0.1 parts;

[0081] Deionized water: 97.5 parts.

[0082] The compound biological enzyme preparation includes urease, protease, lipase and amylase in a mass ratio of 40:20:5:5.

[0083] Preparation method:

[0084] 1) Dissolve OPHB in deionized water, slowly add surfactant, stir at low speed to ensure that no excessive foaming occurs, and stir at 25°C for 15 minutes until evenly dispersed;

[0085] 2) Add baking soda and continue stirring until completely dissolved, then adjust the pH to 6.8;

[0086] 3) Add the compound biological enzyme preparation and plant essential oil at a temperature below 30°C, and stir for 15 minutes;

[0087] 4) Filter the above mixture, fill it into the spraying device, and label it to obtain the finished product.

[0088] The spraying device uses a manually press-down bottle (without propellant), and the nozzle is equipped with a microporous atomizing nozzle (particle size 50-150μm).

[0089] Example 3: Preparation of a long-lasting antibacterial and deodorizing spray

[0090] formula:

[0091] Trihydroxybutyrate oligomer (OPHB): 1.5 parts;

[0092] Compound biological enzyme preparation: 1.5 parts;

[0093] White tea essential oil: 0.15 parts;

[0094] Sodium bicarbonate: 0.4 parts;

[0095] Lauryl phosphate: 0.2 parts;

[0096] Deionized water: 96.25 parts.

[0097] The compound biological enzyme preparation includes urease, protease, lipase and amylase in a mass ratio of 60:40:15:10.

[0098] Preparation method:

[0099] 1) Dissolve OPHB in deionized water, slowly add surfactant, stir at low speed to ensure that no excessive foaming occurs, and stir at 25°C for 15 minutes until evenly dispersed;

[0100] 2) Add baking soda and continue stirring until completely dissolved, then adjust the pH to 6.8;

[0101] 3) Add the compound biological enzyme preparation and plant essential oil at a temperature below 30°C, and stir for 15 minutes;

[0102] 4) Filter the above mixture, fill it into the spraying device, and label it to obtain the finished product.

[0103] The spraying device uses a manually press-down bottle (without propellant), and the nozzle is equipped with a microporous atomizing nozzle (particle size 50-150μm).

[0104] Example 4: The digestion of ammonia in a sealed environment

[0105] A 30cm x 30cm x 30cm transparent acrylic box with an opening at the top is used. An additional acrylic sheet is purchased to cover the opening. A handheld ammonia detector (up to 100ppm) is placed inside the box. A petri dish is placed inside the box for adding concentrated ammonia (20µL). The box is then sealed, and the ammonia concentration is observed. Once the concentration stabilizes, the deodorizing spray obtained in Example 1 is sprayed rapidly at the opening a fixed number of times. The box is then quickly sealed, and the instrument reading is observed to start timing. The time it takes for the ammonia to drop to zero is used as the criterion for judging the quality of the deodorizing spray. The average of three sprays is taken to calculate the odor residue rate.

[0106] Devices such as Figure 1 As shown in the table below, the results are as follows.

[0107]

[0108] Conclusion: This product achieves immediate odor elimination through a biological enzyme decomposition mechanism, with no rebound effect.

[0109] Example 5: Analysis of the odor reduction effect of spraying deodorizing agent on cat litter after adding simulated urine in a sealed environment using gas chromatography-mass spectrometry (GC-MS).

[0110] I. Experimental Objective

[0111] Referring to existing patented technologies for pet odor-removing sprays, this study used gas chromatography-mass spectrometry (GC-MS) under the same sealed conditions as in Example 3 to quantitatively analyze the reduction effect of typical odor-causing volatile organic compounds (VOCs) after adding simulated urine to cat litter and spraying the odor-removing spray of this invention. This verified the scientific validity and effectiveness of its three-in-one mechanism of "sterilization-deodorization-fragrance".

[0112] II. Experimental Materials and Instruments

[0113] 1. Experimental Samples:

[0114] Commercially available bentonite cat litter (unscented, untreated).

[0115] Simulated urine: Prepared according to patent requirements, containing urea (8 g / L), sodium chloride (5 g / L), creatinine (1 g / L), uric acid (0.5 g / L), and ammonia water to adjust the pH to 8.5.

[0116] The present invention is a pet odor-removing spray (prepared in Example 1).

[0117] 2. Instruments and equipment:

[0118] Gas chromatography-mass spectrometry (GC-MS, Agilent 7890B-5977A);

[0119] Headspace sampler (HS-10);

[0120] Incubator (37℃);

[0121] Sealed glass sampling bottle (250 mL, with PTFE sealing gasket).

[0122] Electronic balances, pipettes, etc.

[0123] III. Experimental Methods

[0124] 1. Sample preparation:

[0125] Control group A: Take 50 g of cat litter + 10 mL of deionized water (simulate humidity, without adding urine);

[0126] Control group B: 50 g cat litter + 10 mL simulated urine (without odor remover sprayed);

[0127] Experimental Group C: Take 50 g of cat litter + 10 mL of simulated urine, let stand for 24 hours, and then spray about 15 mL of the odor-removing spray of this invention evenly (spraying distance 25 cm, atomized particle size about 100 μm).

[0128] All samples were placed in sealed glass bottles and incubated at 60°C for 2 hours to promote the release of odors.

[0129] 2. GC-MS detection conditions:

[0130] Chromatographic column: DB-5MS capillary column (30 m × 0.25 mm × 0.25 μm);

[0131] Carrier gas: high-purity helium, flow rate 1.0 mL / min;

[0132] Inlet temperature: 250℃, splitless injection;

[0133] The temperature program is as follows: initially 40℃ and hold for 3 min, then increase to 200℃ at 5℃ / min, and then increase to 280℃ at 10℃ / min and hold for 5 min.

[0134] Mass spectrometry conditions: EI source (70 eV), ion source temperature 230℃, quadrupole temperature 150℃, full scan mode (m / z 35–500).

[0135] Headspace conditions: equilibrium temperature 80℃, equilibrium time 30 min, injection loop temperature 100℃, injection time 1.0 min.

[0136] 3. Data Analysis:

[0137] The main odor components (such as ammonia, hydrogen sulfide, indole, skatole, trimethylamine, acetic acid, propionic acid, etc.) were identified by comparison with the NIST standard spectral library.

[0138] Semi-quantitative analysis was performed using the internal standard method (with valeric acid as the internal standard) to calculate the relative content of odor substances in each group.

[0139] Total peak area of ​​odor substances in experimental group C.

[0140]

[0141] IV. Experimental Results

[0142]

[0143] In experimental group C, characteristic peaks of green tea essential oil (such as linalool and geraniol) were detectable in the spectrum, indicating that the fragrance-enhancing function was effective. In control group B, various short-chain fatty acids and nitrogen-containing heterocyclic compounds were detected, consistent with reports of cat urine putrefaction products in the literature.

[0144] V. Conclusion

[0145] 1. The odor-removing spray of this invention has a significant effect on reducing various typical odor components produced by simulated urine in cat litter, with a total odor removal rate of 93.6%, verifying the high efficiency of compound biological enzymes in decomposing organic matter such as proteins, fats, and uric acid.

[0146] 2. GC-MS analysis confirmed that the elimination of odor was not a "masking" but a source decomposition achieved through biodegradation, consistent with the "odor removal mechanism" described in the patent.

[0147] Example 6 Application Testing: Long-term Effect Verification in Multi-cat Household Scenarios

[0148] Scenario description: The long-term effects of this product will be tested in 5 cat-owning households (each household owns 1-4 cats).

[0149] method:

[0150] Spray twice every two days (once in the morning and once in the evening), using 10-15 mL per liter of cat litter (spraying distance 25 cm).

[0151] Use continuously for 14 days, monitoring indicators include:

[0152] Odor rating: Daily blind evaluation by a third-party sensory evaluation team (0-5 points, 0 for no odor, 5 for strong odor).

[0153] Total bacterial count: Samples were collected from the surface of the cat litter using sterile cotton swabs and counted after 24 hours of incubation.

[0154] Cat behavior observation: Record any abnormal behaviors such as refusing to use the litter box or licking the sprayed area.

[0155] result:

[0156] The odor score decreased from the initial 4±0.5 to 1±0.5 (a decrease of about 70%).

[0157] The total bacterial count decreased by 99.5% (from 1.2 × 10⁻⁶). 5 CFU / g decreased to 6.0-9.1×10 2 CFU / g).

[0158] All cats exhibited no abnormal behavior, paw pads were normal, there were no tear stains or redness around the eyes, and the litter box's clumping performance was unaffected.

[0159] Conclusion: This product can maintain a long-lasting antibacterial and deodorizing effect even under high-load conditions, and is safe and non-irritating to cats.

[0160] The embodiments of the present invention have been described in detail above with reference to the accompanying drawings and specific examples. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those skilled in the art, various changes can be made without departing from the spirit of the present invention.

Claims

1. A long-lasting antibacterial and deodorizing spray, characterized in that, The spray comprises the following raw materials in parts by weight: Trihydroxybutyric acid oligomer (OPHB): 0.5–3.0 parts; Compound biological enzyme preparation: 1-1.5 parts; Plant essential oil: 0.1–0.15 parts; Sodium bicarbonate: 0.1–0.5 parts; Surfactant: 0.1-0.2 parts; Deionized water: 90-100 parts; The compound bio-enzyme preparation includes urease, protease, lipase and amylase.

2. The long-lasting antibacterial and deodorizing spray according to claim 1, characterized in that, In the compound biological enzyme preparation, the mass ratio of urease, protease, lipase and amylase is (40-60):(20-40):(5-15):(5-10).

3. The long-lasting antibacterial and deodorizing spray according to claim 1, characterized in that, The spray comprises the following raw materials in parts by weight: Trihydroxybutyric acid oligomer (OPHB): 1-1.5 parts; Compound biological enzyme preparation: 1-1.5 parts; Plant essential oil: 0.1–0.15 parts; Sodium bicarbonate: 0.3–0.4 parts; Surfactant: 0.1-0.2 parts; Deionized water: 90-100 parts.

4. The long-lasting antibacterial and deodorizing spray according to claim 1, characterized in that, The plant essential oil is selected from one or more of the following: green tea essential oil, white tea essential oil, jasmine essential oil, and lavender essential oil.

5. The long-lasting antibacterial and deodorizing spray according to claim 1, characterized in that, The surfactant is one or more of lauryl phosphate and aminomethylpropanol.

6. The method for preparing the long-lasting antibacterial and deodorizing spray according to any one of claims 1-5, characterized in that, Includes the following steps: 1) Dissolve OPHB in deionized water, slowly add the surfactant, and stir at low speed until evenly dispersed; 2) Add sodium bicarbonate and continue stirring until completely dissolved, then adjust the pH to 6.5-7.2; 3) Add compound biological enzyme preparation and plant essential oil at a temperature below 30°C and stir to obtain a mixture; 4) Filter the above mixture and fill it into the spraying device to obtain the finished product.

7. The preparation method of the long-lasting antibacterial and deodorizing spray according to claim 6, characterized in that, In step 1), the low-speed stirring is performed at 20-30°C for 10-20 minutes.

8. The preparation method of the long-lasting antibacterial and deodorizing spray according to claim 6, characterized in that, In step 3), the stirring time is 10-20 minutes.

9. The application of the long-lasting antibacterial and deodorizing spray according to any one of claims 1-5 in the disinfection and deodorization of pet cat litter.

10. The application according to claim 9, characterized in that, The amount of the spray is 10-20 mL per liter of pet cat litter.

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