Screening method of hydrolytic acidification bacteria, and compounding method and application of bacterial agent thereof

By screening and compounding highly efficient hydrolysis and acidification strains, the problem of insufficient microbial activity in the hydrolysis and acidification process was solved, achieving efficient pretreatment of recalcitrant wastewater and improving its biodegradability.

CN122357679APending Publication Date: 2026-07-10LIAONING UNIVERSITY
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Patent Information

Application Number
CN202610504841.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-16
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

Existing hydrolysis acidification processes suffer from insufficient microbial activity, a simple microbial community structure, poor adaptability to complex substrates, and low hydrolysis efficiency, making it difficult to effectively treat high-concentration, recalcitrant wastewater.

Method used

A screening method for hydrolytic acidifying bacteria was adopted. Through enrichment culture, gradient dilution, specific screening and compounding, highly efficient starch, protein and oil degrading strains were obtained and combined into a hydrolytic acidifying bacterial agent for the treatment of recalcitrant organic wastewater.

Benefits of technology

It achieves simultaneous and directional degradation of three substrates, significantly improves the biodegradability of wastewater, adapts to complex working conditions, and is suitable for large-scale promotion.

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Abstract

This invention belongs to the field of microbial engineering and wastewater treatment technology, specifically relating to a screening method for hydrolytic acidifying bacteria, a method for compounding bacterial agents, and their application. This invention involves screening hydrolytic acidifying bacteria from a sludge-water mixture in a hydrolysis acidification tank, constructing a combination of strains with synergistic degradation effects, preparing a compound bacterial agent, and applying it to wastewater with poor biodegradability. The main steps include: sample sourcing and treatment, initial strain screening, specific screening of functional strains, compounding of bacterial agents, application in hydrolysis acidification, and capacity testing. The screening and compounding method of this invention provides a reliable way to improve the biodegradability of recalcitrant wastewater and has promising prospects for water treatment applications.
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