Engineered adenosine kinase variants
By engineering adenosine kinase and introducing specific amino acid sequence substitutions, the incompatibility problem in the enzyme cascade reaction was solved, the conversion efficiency of nucleoside substrates was improved, and the homeostasis of intracellular nucleotide levels was maintained.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- CODEXIS INC
- Filing Date
- 2024-10-11
- Publication Date
- 2026-07-10
AI Technical Summary
Existing adenosine kinases have incompatibility issues in enzyme cascade reactions, affecting reaction efficiency and stability, and making it difficult to effectively maintain nucleotide levels and concentration gradients across cell membranes.
By engineering adenosine kinase and introducing substitutions or sets of substitutions for specific amino acid sequences, its properties can be improved to meet the requirements of enzyme cascade reactions, thereby enhancing its efficiency in converting nucleoside substrates into nucleoside monophosphates.
It improves the properties of adenosine kinase, increases the efficiency of nucleoside substrate conversion to nucleoside monophosphate, solves the incompatibility problem in enzyme cascade reactions, and maintains the homeostasis of intracellular nucleotide levels.
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