Primary culture device for tumor cells

Abstract

The application discloses a tumor cell primary culture device, which comprises a cell culture bin, a transfer mechanism and a plurality of cell culture bottles. The cell culture bin comprises a culture bin main body, which is internally provided with a plurality of gas chambers which are arranged at intervals along a horizontal preset direction. Any two adjacent gas chambers are provided with an isolation chamber. Each gas chamber is provided with a first air inlet pipeline, a second air inlet pipeline and a pressure relief pipeline. The isolation chamber is provided with a sealed state, a first communication state and a second communication state. The transfer mechanism is arranged outside the culture bin main body and comprises a moving frame, a moving part and an electromagnet. The moving frame is movable along the horizontal preset direction. The moving part is connected to the moving frame and is movable in the longitudinal direction. The electromagnet is connected to the moving part. One side of the cell culture bottle is provided with a first metal sheet. The moving part can adsorb the cell culture bottle in the gas chamber through the electromagnetic force between the electromagnet and the first metal sheet.

Description

Technical Field

[0001] This application relates to the field of cell culture technology, and in particular to a primary tumor cell culture device. Background Technology

[0002] The Warburg effect in tumor cells refers to the process by which tumor cells preferentially obtain energy by breaking down glucose into lactic acid through glycolysis, even under oxygen-sufficient conditions. Tumor tissues often suffer from oxygen deficiency due to vascular abnormalities. Glycolysis is oxygen-independent, which facilitates their survival in harsh environments. Furthermore, the rate of glycolysis is much higher than that of mitochondrial oxidative phosphorylation, thus providing a large amount of ATP (adenosine triphosphate) in a short time to meet the needs of rapid tumor cell division. Therefore, an anaerobic microenvironment with sufficient carbon dioxide concentration is typically used in the culture of tumor cells to meet their rapid division requirements.

[0003] When culturing tumor cells using existing cell culture chambers, each chamber can only provide a microenvironment with the same carbon dioxide concentration. After the tumor cells have evolved, the current carbon dioxide concentration is no longer sufficient to meet their growth requirements. Therefore, it is necessary to transfer the evolved tumor cells to a chamber with a higher carbon dioxide concentration for continued iterative culture.

[0004] However, each transfer of tumor cells severely affects the carbon dioxide concentration in the current incubator, leading to a large consumption of carbon dioxide gas, which significantly increases the cost of tumor cell culture. At the same time, it also requires operators to perform a lot of repetitive work such as transferring cells and replenishing carbon dioxide gas. Summary of the Invention

[0005] The purpose of this application is to provide a primary tumor cell culture device to achieve lower cost and higher efficiency in culturing tumor cells.

[0006] To achieve the above objectives, this application provides the following technical solution: This application discloses a primary tumor cell culture device, which includes a cell culture chamber, a transfer mechanism, and several cell culture flasks. The cell culture chamber includes a main body, and the interior of the main body is provided with multiple gas chambers spaced apart along a predetermined horizontal direction. An isolation chamber is provided between any two adjacent gas chambers. Each gas chamber is provided with a first air inlet pipe, a second air inlet pipe, and a pressure relief pipe that can communicate with the outside of the main body. Each isolation chamber has a sealed state, a first connected state, and a second connected state. The transfer mechanism is located outside the main body of the culture chamber and includes a movable frame, a movable component, and an electromagnet. The movable frame is located on one side of the main body of the culture chamber and can move along a predetermined horizontal direction. The device reciprocates relative to the cell culture chamber. The moving part is connected to the moving frame and can reciprocate longitudinally relative to the moving frame. An electromagnet is connected to the moving part and is located on the side of the moving part facing the main body of the culture chamber. A first metal plate is provided on the side of the cell culture flask facing the moving part. When the electromagnet is energized, the moving part can adsorb the cell culture flask placed in the gas chamber through the electromagnetic force between the electromagnet and the first metal plate. Wherein: when the isolation chamber is in a sealed state, the isolation chamber is not connected to the gas chambers on both sides; when the isolation chamber is in a first connected state, the isolation chamber is connected to the gas chamber on one side; when the isolation chamber is in a second connected state, the isolation chamber is connected to the gas chamber on the other side.

[0007] In one embodiment, in each gas chamber: a gas concentration detector is installed inside the gas chamber to detect the concentration of carbon dioxide, and the gas concentration detector has a preset concentration threshold; a first air inlet pipe is connected to a carbon dioxide gas source outside the culture chamber body through a first solenoid valve; a second air inlet pipe is connected to a nitrogen gas source outside the culture chamber body through a second solenoid valve; a pressure relief pipe is connected to the outside of the culture chamber body through a pressure detector and a third solenoid valve, and the pressure detector has a preset pressure threshold; the gas concentration detector is electrically connected to the first, second, and third solenoid valves respectively, and the pressure detector is electrically connected to the first, second, and third solenoid valves respectively; when the gas pressure in the gas chamber is lower than the preset pressure threshold, the first and second solenoid valves are in a connected state, and the third solenoid valve is in a closed state; when the gas pressure in the gas chamber is higher than the preset pressure threshold, the first and second solenoid valves are in a closed state, and the third solenoid valve is in a connected state; when the carbon dioxide concentration in the gas chamber is lower than the preset concentration threshold, the first solenoid valve is in a connected state, and the second solenoid valve is in a closed state.

[0008] In one embodiment, each isolation chamber includes a first isolation wall, a second isolation wall, and a third isolation wall, and the interior of each isolation chamber includes a first sealing cavity and a second sealing cavity; in each isolation chamber: the first isolation wall, the second isolation wall, and the third isolation wall extend from the bottom inner wall of the culture chamber body toward the top inner wall of the culture chamber body, and the ends of the three extending in the direction of extension are respectively spaced at a predetermined distance from the top inner wall of the culture chamber body; the first isolation wall, the second isolation wall, and the third isolation wall are arranged sequentially at intervals along a predetermined horizontal direction, so that a first sealing cavity is formed between the first isolation wall and the second isolation wall, and a second sealing cavity is formed between the second isolation wall and the third isolation wall; when the isolation chamber is in a sealed state, the first sealing cavity and the second sealing cavity are not connected to their corresponding gas chambers; when the isolation chamber is in a first connected state, the first sealing cavity is connected to its corresponding gas chamber, and the second sealing cavity is not connected to its corresponding gas chamber; when the isolation chamber is in a second connected state, the first sealing cavity is not connected to its corresponding gas chamber, and the second sealing cavity is connected to its corresponding gas chamber.

[0009] In one embodiment, each isolation chamber further includes a fourth isolation wall and a fifth isolation wall, and the interior of each isolation chamber also includes a third sealing cavity; in each isolation chamber: the fourth isolation wall and the fifth isolation wall extend from the top inner wall of the culture chamber body toward the bottom inner wall of the culture chamber body, and the ends of their extension directions are respectively spaced at a predetermined distance from the bottom inner wall of the culture chamber body; the fourth isolation wall is located between the first isolation wall and the second isolation wall, and the fifth isolation wall is located between the second isolation wall and the third isolation wall, so that a third sealing cavity is formed between the fourth isolation wall and the fifth isolation wall, and the third sealing cavity is connected to the first sealing cavity and the second sealing cavity respectively; when the isolation chamber is in a sealed state, the first sealing cavity and the second sealing cavity are not connected to their corresponding gas chambers; when the isolation chamber is in a first connected state, the third sealing cavity is connected to the gas chamber corresponding to the first sealing cavity through the first sealing cavity, and the second sealing cavity is not connected to its corresponding gas chamber; when the isolation chamber is in a second connected state, the first sealing cavity is not connected to its corresponding gas chamber, and the third sealing cavity is connected to the gas chamber corresponding to the second sealing cavity through the second sealing cavity.

[0010] In one embodiment, in each isolation chamber: a first sealed chamber contains a first liquid medium, and a second sealed chamber contains a second liquid medium; when the isolation chamber is in a sealed state, the liquid level of the first liquid medium is higher than the bottom of the fourth isolation wall, and the liquid level of the second liquid medium is higher than the bottom of the fifth isolation wall, so that the first sealed chamber, the second sealed chamber, and the third sealed chamber are not in communication with each other; when the isolation chamber is in a first connected state, the liquid level of the first liquid medium is lower than the bottom of the fourth isolation wall, and the liquid level of the second liquid medium is higher than the bottom of the fifth isolation wall, so that the first sealed chamber is in communication with the third sealed chamber, and the second sealed chamber is not in communication with the third sealed chamber; when the isolation chamber is in a second connected state, the liquid level of the first liquid medium is higher than the bottom of the fourth isolation wall, and the liquid level of the second liquid medium is lower than the bottom of the fifth isolation wall, so that the first sealed chamber is not in communication with the third sealed chamber, and the second sealed chamber is in communication with the third sealed chamber.

[0011] In one embodiment, the culture chamber body further includes a liquid storage chamber for containing liquid media. A liquid pump and a fourth solenoid valve are installed within the liquid storage chamber, with the pumping line of the liquid pump connected to the fourth solenoid valve. In each isolation chamber: a first inlet pipe and a first outlet pipe are installed in the first sealed chamber; the first inlet pipe is connected to the fourth solenoid valve, and the first outlet pipe is connected to the liquid storage chamber via a fifth solenoid valve. A second inlet pipe and a second outlet pipe are installed in the second sealed chamber; the second inlet pipe is connected to the fourth solenoid valve, and the second outlet pipe is connected to the liquid storage chamber via a sixth solenoid valve. The liquid pump pumps liquid media into the first and second inlet pipes respectively via the fourth solenoid valve. After flowing into the first and second sealed chambers, the liquid media forms the first liquid media and the second liquid media respectively.

[0012] In one embodiment, a second metal plate is provided at the bottom of each cell culture flask, a first proximity switch is provided at the top of the first isolation wall corresponding to the second metal plate, a second proximity switch is provided at the top of the second isolation wall corresponding to the second metal plate, and a third proximity switch is provided at the top of the third isolation wall corresponding to the second metal plate; a first liquid level sensor and a second liquid level sensor are arranged longitudinally at intervals inside the first sealed cavity, and the bottom end of the fourth isolation wall is located between the first liquid level sensor and the second liquid level sensor; a third liquid level sensor and a fourth liquid level sensor are arranged longitudinally at intervals inside the second sealed cavity, and the bottom end of the fifth isolation wall is located between the third liquid level sensor and the fourth liquid level sensor; the first proximity switch and the second liquid level sensor are electrically connected to the fifth solenoid valve respectively, the second proximity switch, the first liquid level sensor, and the third liquid level sensor are electrically connected to the fourth solenoid valve respectively, and the third proximity switch and the third liquid level sensor are electrically connected to the sixth solenoid valve respectively: when the first proximity switch, the second proximity switch, and the third proximity switch are not triggered, the isolation chamber is in a sealed state; when the second proximity switch is not triggered, the isolation chamber is sealed; when the second proximity switch is not triggered, the isolation chamber is sealed. When the metal strip triggers the first proximity switch, the fifth solenoid valve is activated, and the first liquid medium flows into the storage chamber through the first outlet pipe. When the second liquid level sensor is triggered, the fifth solenoid valve is closed, and the isolation chamber is in the first connected state, connecting the first and third sealed chambers. When the second metal strip triggers the second proximity switch, the fourth solenoid valve is activated, and the liquid medium flows into the first sealed chamber through the first inlet pipe. When the first liquid level sensor is triggered, the fourth solenoid valve is closed, and the isolation chamber is in a sealed state. When the second metal strip triggers the second proximity switch and the first liquid level sensor is triggered, the sixth solenoid valve is activated, and the second liquid medium flows into the storage chamber through the second outlet pipe. When the fourth liquid level sensor is triggered, the sixth solenoid valve is closed, and the isolation chamber is in the second sealed state, connecting the second and third sealed chambers. When the second metal strip triggers the third proximity switch, the fourth solenoid valve is activated, and the liquid medium flows into the second sealed chamber through the second inlet pipe. When the third liquid level sensor is triggered, the fourth solenoid valve is closed, and the isolation chamber is in a sealed state.

[0013] In one embodiment, the interior of each gas chamber further includes several uniformly arranged culture flask racks, each culture flask rack being equipped with a fourth proximity switch; and each cell culture flask body being equipped with a third metal plate corresponding to the fourth proximity switch.

[0014] In one embodiment, the transfer mechanism further includes a first drive motor and a first lead screw. The first lead screw extends along a horizontal preset direction and is connected to the output shaft of the first drive motor. The movable frame is connected to the first lead screw so that the first drive motor can drive the movable frame to reciprocate along the horizontal preset direction through the first lead screw.

[0015] In one embodiment, the transfer mechanism further includes a second drive motor and a second lead screw. The second lead screw is disposed on the movable frame and extends longitudinally. The second lead screw is connected to the output shaft of the second drive motor, and the movable member is connected to the second lead screw so that the second drive motor can drive the movable member to reciprocate longitudinally through the second lead screw.

[0016] Analysis reveals that this invention discloses a primary tumor cell culture device. During use, multiple gas chambers arranged at intervals can have progressively adjusted carbon dioxide gas concentrations. Each gas chamber maintains a constant internal carbon dioxide gas concentration and pressure through a first inlet pipe, a second inlet pipe, and a pressure relief pipe. Isolation chambers between any two adjacent gas chambers are cyclically switched between a sealed state, a first connected state, and a third connected state via a liquid medium. Electromagnets in the transfer mechanism allow cell culture flasks within the gas chambers to be transferred from outside the cell culture chamber. When tumor cells divide to the upper limit of the current microenvironment, the cell culture flask can be transferred to the next gas chamber with a higher carbon dioxide concentration without opening the chamber door to continue cell culture. This effectively solves the technical problem of excessive carbon dioxide consumption due to cell culture flask transfer, significantly reducing the cost of tumor cell culture and minimizing repetitive labor for operators during cell transfer. Attached Figure Description

[0017] The accompanying drawings, which form part of this application, are used to provide a further understanding of this application. The illustrative embodiments and descriptions of this application are used to explain this application and do not constitute an undue limitation of this application. Wherein: Figure 1 A schematic diagram of the structure of the primary tumor cell culture device provided in the embodiments of this application within a cell culture incubator; Figure 2 A schematic diagram of the structure of the cell culture chamber in the primary tumor cell culture device provided for embodiments of this application; Figure 3 A schematic cross-sectional view of the cell culture chamber in a primary tumor cell culture apparatus provided for embodiments of this application; Figure 4 for Figure 3 A partially enlarged structural diagram of section A in the middle; Figure 5 for Figure 3 A partially enlarged structural diagram of section B in the middle section; Figure 6 for Figure 3 A partially enlarged structural diagram of section C in the middle; Figure 7 A schematic diagram of the transfer mechanism in a primary tumor cell culture device provided for an embodiment of this application, viewed from a first angle; Figure 8 for Figure 7 A partially enlarged structural diagram of section D in the middle section; Figure 9 A schematic diagram of the transfer mechanism in a primary tumor cell culture device provided for embodiments of this application, viewed from a second angle; Figure 10 A schematic diagram of the structure of a cell culture flask in a primary tumor cell culture apparatus provided for an embodiment of this application.

[0018] Explanation of reference numerals in the attached figures: 1. Cell culture chamber; 101. Culture chamber body; 102. Gas chamber; 102a. First gas inlet pipe; 102b. Gas concentration detector; 102c. First solenoid valve; 102d. Second gas inlet pipe; 102e. Second solenoid valve; 102f. Pressure relief pipe; 102g. Gas pressure detector; 102h. Third solenoid valve; 102i. Culture flask rack; 102j. Fourth proximity switch; 102k. Chamber door; 103. Isolation chamber; 103a. First sealed chamber; 10 3b. Third sealing cavity; 103c. Second sealing cavity; 103d. First isolation wall; 103e. Second isolation wall; 103f. Third isolation wall; 103g. Fourth isolation wall; 103h. Fifth isolation wall; 103i. First proximity switch; 103j. Second proximity switch; 103k. Third proximity switch; 103l. First liquid level sensor; 103m. Second liquid level sensor; 103n. Third liquid level sensor; 103o. Fourth liquid level sensor; 103p. First liquid inlet. Piping; 103q, First outlet pipe; 103r, Fifth solenoid valve; 103s, Second inlet pipe; 103t, Second outlet pipe; 103u, Sixth solenoid valve; 104, Storage chamber; 104a, Injection pipe; 104b, Drainage pipe; 105, Pump; 105a, Pumping pipe; 106, Fourth solenoid valve; 2, Transfer mechanism; 201, Moving frame; 202, Moving part; 203, Electromagnet; 204, First drive motor; 205, First lead screw; 206. 207. First transmission nut; 208. First guide rail; 209. First slider; 210. Second drive motor; 211. Second lead screw; 212. Second transmission nut; 213. Guide rod; 214. Guide slider; 215. Connector; 216. Second guide rail; 217. Second slider; 3. Cell culture chamber; 4. Cell culture flask; 401. Flask body; 402. Flask cap; 403. First metal sheet; 404. Third metal sheet; 405. Second metal sheet; X: Horizontal preset direction; Y: Longitudinal direction. Detailed Implementation

[0019] The present application will now be described in detail with reference to the accompanying drawings and embodiments. Various examples are provided by way of explanation and not by way of limitation. In fact, those skilled in the art will recognize that modifications and variations can be made to the present application without departing from the scope or spirit thereof. For example, a feature shown or described as part of one embodiment may be used in another embodiment to produce yet another embodiment. Therefore, it is desirable that the present application encompass such modifications and variations that fall within the scope of the appended claims and their equivalents.

[0020] In the description of this application, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," and "bottom," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this application and do not require that this application be constructed and operated in a specific orientation, and therefore should not be construed as limiting this application. The terms "connected," "linked," and "set up" used in this application should be interpreted broadly. For example, they can refer to fixed connections or detachable connections; direct connections or indirect connections through intermediate components; wired connections, radio connections, or wireless communication signal connections. Those skilled in the art can understand the specific meaning of the above terms according to the specific circumstances.

[0021] The accompanying drawings illustrate one or more examples of this application. The detailed description uses numerical and alphabetic designations to refer to features in the drawings. Similar or analogous designations in the drawings and description have been used to refer to similar or analogous parts of this application. As used herein, the terms “first,” “second,” and “third,” etc., are used interchangeably to distinguish one component from another and are not intended to indicate the location or importance of individual components.

[0022] like Figures 1 to 10 As shown in the embodiments of this application, a primary tumor cell culture device is provided, including at least one cell culture chamber 1, a transport mechanism, and a plurality of cell culture flasks 4 whose functions are matched with the above two. This culture device can be used independently or as... Figure 1 As shown, place it in an existing cell culture incubator 3 for use.

[0023] Specifically, with Figures 2 to 6 For example, the cell culture chamber 1 has four gas chambers 102 inside its main body 101. The four gas chambers 102 are spaced apart along a preset horizontal direction X. Each gas chamber 102 is provided with an independent door 102k. An isolation chamber 103 is provided between any two adjacent gas chambers 102.

[0024] Each gas chamber 102 is provided with a first air inlet pipe 102a, a second air inlet pipe 102d and a pressure relief pipe 102f. Each gas chamber 102 is provided with a gas concentration detector 102b for detecting the carbon dioxide concentration inside the gas chamber 102 and a pressure detector 102g for detecting the gas pressure inside the gas chamber 102. In each gas chamber 102, a first inlet pipe 102a is connected to a carbon dioxide gas source outside the culture chamber body 101 via a first solenoid valve 102c, allowing carbon dioxide gas to be introduced into the gas chamber 102 through the first solenoid valve 102c and the first inlet pipe 102a; a second inlet pipe 102d is connected to a nitrogen gas source outside the culture chamber body 101 via a second solenoid valve 102e, allowing nitrogen gas to be introduced into the gas chamber 102 through the second solenoid valve 102e and the second inlet pipe 102d; a pressure relief pipe 102f is connected to the outside of the culture chamber body 101 via a pressure detector 102g and a third solenoid valve 102h, allowing gas in the gas chamber 102 to be discharged to the outside of the culture chamber body 101 through the third solenoid valve 102h and the pressure relief pipe 102f, thereby maintaining the internal pressure of the gas chamber 102.

[0025] In each gas chamber 102, a gas concentration detector 102b has a preset carbon dioxide concentration threshold, and a gas pressure detector 102g has a preset pressure threshold. When the gas pressure in the gas chamber 102 is lower than the preset pressure threshold, the first solenoid valve 102c and the second solenoid valve 102e are in a connected state, and the third solenoid valve 102h is in a closed state, so that the first inlet pipe 102a can introduce carbon dioxide gas into the gas chamber 102, and the second inlet pipe 102d can introduce nitrogen gas into the gas chamber 102, thereby maintaining the gas pressure in the gas chamber 102 at the preset pressure threshold; when the gas pressure in the gas chamber 102 is higher than the preset pressure threshold, the first solenoid valve 102c and the second solenoid valve 102e... When the gas chamber 102 is in a closed state, the third solenoid valve 102h is in a connected state, so that the gas chamber 102 can discharge gas through the pressure relief pipe 102f, thereby maintaining the gas pressure in the gas chamber 102 at a preset pressure threshold. When the carbon dioxide concentration in the gas chamber 102 is lower than the preset carbon dioxide concentration threshold, the first solenoid valve 102c is in a connected state and the second solenoid valve 102e is in a closed state, so that the first air inlet pipe 102a can introduce carbon dioxide gas into the gas chamber 102, thereby maintaining the carbon dioxide concentration in the gas chamber 102.

[0026] Each isolation chamber 103 is composed of a first isolation wall 103d, a second isolation wall 103e, a third isolation wall 103f, a fourth isolation wall 103g, and a fifth isolation wall 103h. The first isolation wall 103d, the second isolation wall 103e, and the third isolation wall 103f extend from the bottom inner wall of the culture chamber body 101 toward the top inner wall of the culture chamber body 101, and the ends of the three extending in the direction of extension are respectively spaced at a predetermined distance from the top inner wall of the culture chamber body 101. The first isolation wall 103d, the second isolation wall 103e, and the third isolation wall 103f are arranged sequentially at intervals along a predetermined horizontal direction X, so that a first sealing cavity 103a is formed between the first isolation wall 103d and the second isolation wall 103e, and a second sealing cavity 103c is formed between the second isolation wall 103e and the third isolation wall 103f. The fourth isolation wall 103g and the fifth isolation wall 103h extend from the top inner wall of the culture chamber body 101 toward the bottom inner wall of the culture chamber body 101, and the ends of the two extending in the direction of extension are respectively spaced at a predetermined distance from the bottom inner wall of the culture chamber body 101. The fourth isolation wall 103g is located between the first isolation wall 103d and the second isolation wall 103e, and the fifth isolation wall 103h is located between the second isolation wall 103e and the third isolation wall 103f, so that a third sealing cavity 103b is formed between the fourth isolation wall 103g and the fifth isolation wall 103h, and the third sealing cavity 103b is connected to the first sealing cavity 103a and the second sealing cavity 103c, respectively.

[0027] The liquid storage chamber 104 inside the culture chamber body 101 is used to contain liquid medium, which can be distilled water. The liquid storage chamber 104 is equipped with a liquid pump 105, a liquid injection pipeline 104a for injecting liquid medium into the liquid storage chamber 104, and a liquid discharge pipeline 104b for discharging liquid medium from the liquid storage chamber 104. In each isolation chamber 103, a first inlet pipe 103p and a first outlet pipe 103q are provided in the first sealed chamber 103a. The first inlet pipe 103p is connected to the pumping pipe 105a of the liquid pump 105 through a fourth solenoid valve 106, and the first outlet pipe 103q is connected to the storage chamber 104 through a fifth solenoid valve 103r. A second inlet pipe 103s and a second outlet pipe 103t are provided in the second sealed chamber 103c. The second inlet pipe 103s is connected to the pumping pipe 105a of the liquid pump 105 through a fourth solenoid valve 106, and the second outlet pipe 103t is connected to the storage chamber 104 through a sixth solenoid valve 103u.

[0028] The transfer mechanism 2 includes a movable frame 201, a movable component 202, and an electromagnet 203. The movable frame 201 is located on the side of the culture chamber body 101 facing away from the chamber door 102k, and is screwed to the first lead screw 205 via a first transmission nut 206, and slidably connected to the first guide rail 207 via a first slider 208. The first lead screw 205 and the first guide rail 207 are spaced apart and extend along a predetermined horizontal direction X. The first lead screw 205 is connected to the output shaft of the first drive motor 204, so that the first drive motor 204 can drive the movable frame 201 to reciprocate along the predetermined horizontal direction X via the first lead screw 205. The movable component 202 is located between the movable frame 201 and the culture chamber body 101. The movable component 202 is slidably connected to the second guide rail 215 via a second slider 216, and also slidably connected to the guide rod 212 via a guide slider 213. The guide slider 213 is connected to the second transmission nut 211 via a connector 214. The second transmission nut 211 is screwed to the second lead screw 210. The second lead screw 210, the second guide rail 215, and the guide rod 212 extend longitudinally along the Z direction. The second lead screw 210 is connected to the output shaft of the second drive motor 209, so that the second drive motor 209 can drive the movable component 202 to reciprocate longitudinally along the Z direction via the second lead screw 210. An electromagnet 203 is disposed on the movable component 202, so that the movable component 202 can drive the electromagnet 203 to reciprocate longitudinally along the Z direction.

[0029] Each gas chamber 102 is equipped with multiple culture flask racks 102i for placing cell culture flasks 4, and each culture flask rack 102i is equipped with a third proximity switch 103k 102j. In each isolation chamber 103, the top of the first isolation wall 103d is equipped with a first proximity switch 103i, the top of the second isolation wall 103e is equipped with a second proximity switch 103j, and the top of the third isolation wall 103f is equipped with a third proximity switch 103k. The interior of the first sealed chamber 103a is equipped with a first liquid level sensor 103l and a second liquid level sensor 103m spaced along the longitudinal direction Z, and the bottom end of the fourth isolation wall 103g is located between the first liquid level sensor 103l and the second liquid level sensor 103m. The interior of the second sealed chamber 103c is equipped with a first liquid level sensor 103l and a second liquid level sensor 103m spaced along the longitudinal direction Z. A third liquid level sensor 103n and a fourth liquid level sensor 103o are provided, and the bottom end of the fifth isolation wall 103h is located between the third liquid level sensor 103n and the fourth liquid level sensor 103o; the first proximity switch 103i and the second liquid level sensor 103m are electrically connected to the fifth solenoid valve 103r, the second proximity switch 103j, the first liquid level sensor 103l and the third liquid level sensor 103n are electrically connected to the fourth solenoid valve 106, and the third proximity switch 103k and the third liquid level sensor 103n are electrically connected to the sixth solenoid valve 103u.

[0030] In each cell culture flask 4, a first metal plate 403 is provided on the side of the flask body 401 facing away from the cap 402, corresponding to the electromagnet 203. When the electromagnet 203 is energized, the moving part 202 can adsorb the cell culture flask 4 placed in the gas chamber 102 through the electromagnetic force between the electromagnet 203 and the first metal plate 403. A second metal plate 405 is provided at the bottom of the flask body 401. During the transfer process of the cell culture flask 4 driven by the transfer mechanism 2, the second metal plate 405 can trigger the first proximity switch 103i, the second proximity switch 103j, and the third proximity switch 103k respectively. A third metal plate 404 is provided on one side of the flask body 401, corresponding to the third proximity switch 103k102j, so that the third proximity switch 103k102j can detect the placement of the cell culture flask 4 in the cell culture chamber 1 through the third metal plate 404.

[0031] In each isolation chamber 103, during the transfer process where the transfer mechanism 2 drives the cell culture flask 4 via the electromagnet 203: when the second metal plate 405 does not trigger the first proximity switch 103i, the second proximity switch 103j, and the third proximity switch 103k, the isolation chamber 103 is in the sealed state, and the gas chamber 102 where the cell culture flask 4 is located is not connected to either the first sealed chamber 103a or the second sealed chamber 103c; when the second metal plate 405 triggers the first proximity switch 103i, the fifth solenoid valve 103r is connected, and the first liquid medium in the first sealed chamber 103a flows into the storage chamber 104 through the first outlet pipe 103q; when the liquid level of the first liquid medium is detected by the second liquid level sensor 103m... When the fifth solenoid valve 103r is closed, the isolation chamber 103 is in the first connected state. At this time, the current gas chamber 102 is connected to the third sealed chamber 103b through the first sealed chamber 103a. The cell culture flask 4 can be moved to the third sealed chamber 103b via the electromagnet 203 through the first sealed chamber 103a. When the second metal plate 405 triggers the second proximity switch 103j, the fourth solenoid valve 106 connects to the first liquid inlet pipe 103p. The liquid medium in the storage chamber 104 flows into the first sealed chamber 103a through the liquid pump 105 and the first liquid inlet pipe 103p. When the liquid level of the first liquid medium in the first sealed chamber 103a is detected by the first liquid level sensor 103l, the fourth solenoid valve 106 is closed, and the isolation chamber 103 is in the first connected state. 03 is in the sealed state, at which time neither the first sealing cavity 103a nor the second sealing cavity 103c is connected to the third sealing cavity 103b; when the second metal plate 405 triggers the second proximity switch 103j, and the liquid level of the first liquid medium in the first sealing cavity 103a is detected by the first liquid level sensor 103l, the sixth solenoid valve 103u is connected, and the second liquid medium in the second sealing cavity 103c flows into the storage chamber 104 through the second liquid outlet pipe 103t; when the second liquid medium is detected by the fourth liquid level sensor 103o, the sixth solenoid valve 103u is closed, and the isolation chamber 103 is in the second sealed state. At this time, the third sealing cavity 103b is connected to the next gas chamber 102 through the second sealing cavity 103c. The cell culture flask 4 can be moved to the next gas chamber 102 via the second sealed chamber 103c through the electromagnet 203. When the second metal plate 405 triggers the third proximity switch 103k, the fourth solenoid valve 106 connects the second liquid inlet pipe 103s. The liquid medium in the liquid storage chamber 104 flows into the second sealed chamber 103c through the liquid pump 105 and the second liquid inlet pipe 103s. When the second liquid medium in the second sealed chamber 103c is detected by the third liquid level sensor 103n, the fourth solenoid valve 106 closes, and the isolation chamber 103 is in a sealed state. At this time, the first sealed chamber 103a, the second sealed chamber 103c, the third sealed chamber 103b, and the gas chambers 102 on both sides of the isolation chamber 103 are not connected to each other.

[0032] As can be seen from the above description, the embodiments of the present invention achieve the following technical effects: The primary tumor cell culture device provided in the above embodiments of this application allows for stepped carbon dioxide gas concentration settings within a plurality of gas chambers 102 arranged at intervals. Each gas chamber 102 maintains a constant internal carbon dioxide gas concentration and pressure through a first inlet pipe 102a, a second inlet pipe 102d, and a pressure relief pipe 102f. An isolation chamber 103 between any two adjacent gas chambers 102 achieves cyclic switching between a sealed state, a first connected state, and a third connected state via a liquid medium. The cell culture flask 4 within the gas chambers 102 can be transferred from outside the cell culture chamber 1 using an electromagnet 203 in the transfer mechanism 2. When tumor cells divide to the upper limit of the current microenvironment culture, the cell culture flask 4 can be transferred to the next gas chamber 102 with a higher carbon dioxide gas concentration without opening the chamber door 102k to continue cell culture. This effectively solves the technical problem of excessive carbon dioxide gas consumption due to cell culture flask 4 transfer, significantly reducing the cost of tumor cell culture and minimizing repetitive labor for operators during cell transfer.

[0033] The above are merely some embodiments of this application and are not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A primary tumor cell culture device, characterized in that, include: A cell culture chamber includes a culture chamber body. The culture chamber body has multiple gas chambers spaced apart along a predetermined horizontal direction inside. An isolation chamber is provided between any two adjacent gas chambers. Each gas chamber is provided with a first air inlet pipe, a second air inlet pipe, and a pressure relief pipe that can communicate with the outside of the culture chamber body. Each isolation chamber has a sealed state, a first connected state, and a second connected state. A transfer mechanism is disposed outside the main body of the culture chamber. It includes a movable frame, a movable component, and an electromagnet. The movable frame is located on one side of the main body of the culture chamber and can reciprocate relative to the cell culture chamber along the horizontal preset direction. The movable component is connected to the movable frame and can reciprocate relative to the movable frame along the longitudinal direction. The electromagnet is connected to the movable component and is located on the side of the movable component facing the main body of the culture chamber. as well as If the cell culture flask is a stem cell culture flask, a first metal plate is provided on the side of the flask body facing the moving part. When the electromagnet is energized, the moving part can adsorb the cell culture flask placed in the gas chamber through the electromagnetic force between the electromagnet and the first metal plate. in: When the isolation chamber is in the sealed state, the isolation chamber is not in communication with the gas chambers on both sides; When the isolation chamber is in the first connected state, the isolation chamber is connected to the gas chamber on one side of it; When the isolation chamber is in the second connected state, the isolation chamber is connected to the gas chamber on the other side.

2. The primary tumor cell culture device according to claim 1, characterized in that, In each of the gas chambers: The gas chamber is equipped with a gas concentration detector for detecting the concentration of carbon dioxide. The gas concentration detector has a preset concentration threshold. The first air intake pipe is connected to a carbon dioxide gas source outside the culture chamber body through a first solenoid valve; The second air inlet pipe is connected to a nitrogen source outside the culture chamber body via a second solenoid valve; The pressure relief pipeline is connected to the outside of the culture chamber body through a pressure detector and a third solenoid valve. The pressure detector has a preset pressure threshold. The gas concentration detector is electrically connected to the first solenoid valve, the second solenoid valve, and the third solenoid valve, respectively. The gas pressure detector is electrically connected to the first solenoid valve, the second solenoid valve, and the third solenoid valve, respectively. When the gas pressure in the gas chamber is lower than the preset pressure threshold, the first solenoid valve and the second solenoid valve are in a connected state, and the third solenoid valve is in a closed state. When the gas pressure in the gas chamber is higher than the preset pressure threshold, the first solenoid valve and the second solenoid valve are in a closed state, and the third solenoid valve is in a connected state. When the carbon dioxide concentration in the gas chamber is lower than the preset concentration threshold, the first solenoid valve is in the connected state and the second solenoid valve is in the closed state.

3. The primary tumor cell culture device according to claim 1, characterized in that, Each of the isolation chambers includes a first isolation wall, a second isolation wall, and a third isolation wall, and the interior of each of the isolation chambers includes a first sealing cavity and a second sealing cavity; In each of the isolation chambers: The first isolation wall, the second isolation wall, and the third isolation wall extend from the bottom inner wall of the culture chamber body toward the top inner wall of the culture chamber body, and the ends of the three walls in the extension direction are respectively spaced at a predetermined distance from the top inner wall of the culture chamber body; The first isolation wall, the second isolation wall, and the third isolation wall are arranged sequentially at intervals along the predetermined horizontal direction, so that the first isolation wall and the second isolation wall form the first sealing cavity, and the second isolation wall and the third isolation wall form the second sealing cavity; When the isolation chamber is in the sealed state, neither the first sealed chamber nor the second sealed chamber is in communication with its corresponding gas chamber. When the isolation chamber is in the first connected state, the first sealed chamber is connected to its corresponding gas chamber, and the second sealed chamber is not connected to its corresponding gas chamber. When the isolation chamber is in the second connected state, the first sealing chamber and its corresponding gas chamber are not connected to each other, while the second sealing chamber and its corresponding gas chamber are connected.

4. The primary tumor cell culture device according to claim 3, characterized in that, Each of the isolation chambers further includes a fourth isolation wall and a fifth isolation wall, and the interior of each of the isolation chambers further includes a third sealed cavity; In each of the isolation chambers: The fourth and fifth isolation walls extend from the top inner wall of the culture chamber body toward the bottom inner wall of the culture chamber body, and the ends of the two walls in the extension direction are respectively spaced at a predetermined distance from the bottom inner wall of the culture chamber body. The fourth isolation wall is located between the first isolation wall and the second isolation wall, and the fifth isolation wall is located between the second isolation wall and the third isolation wall, so that the third sealing cavity is formed between the fourth isolation wall and the fifth isolation wall, and the third sealing cavity is in communication with the first sealing cavity and the second sealing cavity respectively; When the isolation chamber is in the sealed state, neither the first sealed chamber nor the second sealed chamber is in communication with its corresponding gas chamber. When the isolation chamber is in the first connected state, the third sealing chamber is connected to the gas chamber corresponding to the first sealing chamber through the first sealing chamber, and the second sealing chamber is not connected to its corresponding gas chamber. When the isolation chamber is in the second connected state, the first sealing chamber and its corresponding gas chamber are not connected to each other, and the third sealing chamber is connected to the gas chamber corresponding to the second sealing chamber through the second sealing chamber.

5. The primary tumor cell culture apparatus according to claim 4, characterized in that, In each of the isolation chambers: The first sealed cavity contains a first liquid medium, and the second sealed cavity contains a second liquid medium; When the isolation chamber is in the sealed state, the liquid level of the first liquid medium is higher than the bottom of the fourth isolation wall, and the liquid level of the second liquid medium is higher than the bottom of the fifth isolation wall, so that the first sealing chamber, the second sealing chamber and the third sealing chamber are not in communication with each other; When the isolation chamber is in the first connected state, the liquid level of the first liquid medium is lower than the bottom of the fourth isolation wall, and the liquid level of the second liquid medium is higher than the bottom of the fifth isolation wall, so that the first sealing chamber is connected to the third sealing chamber, and the second sealing chamber is not connected to the third sealing chamber. When the isolation chamber is in the second connected state, the liquid level of the first liquid medium is higher than the bottom of the fourth isolation wall, and the liquid level of the second liquid medium is lower than the bottom of the fifth isolation wall, so that the first sealing chamber is not connected to the third sealing chamber, and the second sealing chamber is connected to the third sealing chamber.

6. The primary tumor cell culture apparatus according to claim 5, characterized in that, The main body of the culture chamber also includes a liquid storage chamber for containing liquid media. The liquid storage chamber is equipped with a liquid pump and a fourth solenoid valve, and the pumping pipeline of the liquid pump is connected to the fourth solenoid valve. In each of the isolation chambers: The first sealed cavity is provided with a first liquid inlet pipe and a first liquid outlet pipe. The first liquid inlet pipe is connected to the fourth solenoid valve, and the first liquid outlet pipe is connected to the liquid storage chamber through a fifth solenoid valve. The second sealing cavity is provided with a second liquid inlet pipe and a second liquid outlet pipe. The second liquid inlet pipe is connected to the fourth solenoid valve, and the second liquid outlet pipe is connected to the liquid storage chamber through a sixth solenoid valve. The liquid pump pumps the liquid medium into the first liquid inlet pipe and the second liquid inlet pipe respectively through the fourth solenoid valve. After the liquid medium flows into the first sealing cavity and the second sealing cavity, it forms the first liquid medium and the second liquid medium respectively.

7. The primary tumor cell culture apparatus according to claim 6, characterized in that, Each cell culture flask is provided with a second metal plate at the bottom, a first proximity switch is provided at the top of the first isolation wall corresponding to the second metal plate, a second proximity switch is provided at the top of the second isolation wall corresponding to the second metal plate, and a third proximity switch is provided at the top of the third isolation wall corresponding to the second metal plate. The first liquid level sensor and the second liquid level sensor are arranged longitudinally at intervals inside the first sealed cavity, and the bottom end of the fourth isolation wall is located between the first liquid level sensor and the second liquid level sensor. The second sealed cavity is provided with a third liquid level sensor and a fourth liquid level sensor arranged longitudinally at intervals, and the bottom end of the fifth isolation wall is located between the third liquid level sensor and the fourth liquid level sensor; The first proximity switch and the second liquid level sensor are electrically connected to the fifth solenoid valve, the second proximity switch, the first liquid level sensor, and the third liquid level sensor are electrically connected to the fourth solenoid valve, and the third proximity switch and the third liquid level sensor are electrically connected to the sixth solenoid valve. When the first proximity switch, the second proximity switch, and the third proximity switch are all not triggered, the isolation chamber is in the sealed state; When the second metal plate triggers the first proximity switch, the fifth solenoid valve is connected, and the first liquid medium flows into the liquid storage chamber through the first liquid outlet pipe. When the second liquid level sensor is triggered, the fifth solenoid valve is closed, the isolation chamber is in the first connected state, and the first sealing chamber is connected to the third sealing chamber. When the second metal plate triggers the second proximity switch, the fourth solenoid valve connects to the first liquid inlet pipe, and the liquid medium flows into the first sealed cavity through the first liquid inlet pipe. When the first liquid level sensor is triggered, the fourth solenoid valve closes, and the isolation chamber is in the sealed state. When the second metal plate triggers the second proximity switch and the first liquid level sensor is triggered, the sixth solenoid valve is connected, and the second liquid medium flows into the storage chamber through the second liquid outlet pipe. When the fourth liquid level sensor is triggered, the sixth solenoid valve is closed, the isolation chamber is in the second sealed state, and the second sealed chamber is connected to the third sealed chamber. When the second metal plate triggers the third proximity switch, the fourth solenoid valve connects to the second liquid inlet pipe, and the liquid medium flows into the second sealed cavity through the second liquid inlet pipe. When the third liquid level sensor is triggered, the fourth solenoid valve closes, and the isolation chamber is in the sealed state.

8. The primary tumor cell culture apparatus according to claim 1, characterized in that, Each of the gas chambers also includes several evenly arranged culture bottle racks, and each culture bottle rack is equipped with a fourth proximity switch; Each of the cell culture flasks has a third metal plate on its body corresponding to the fourth proximity switch.

9. The primary tumor cell culture apparatus according to claim 1, characterized in that, The transfer mechanism further includes a first drive motor and a first lead screw. The first lead screw extends along the preset horizontal direction and is connected to the output shaft of the first drive motor. The movable frame is connected to the first lead screw so that the first drive motor can drive the movable frame to reciprocate along the preset horizontal direction through the first lead screw.

10. The primary tumor cell culture apparatus according to claim 1, characterized in that, The transfer mechanism further includes a second drive motor and a second lead screw. The second lead screw is disposed on the movable frame and extends longitudinally. The second lead screw is connected to the output shaft of the second drive motor. The movable member is connected to the second lead screw so that the second drive motor can drive the movable member to reciprocate longitudinally through the second lead screw.

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