Seed island porphyra-based mutagenesis breeding method and application thereof

By using ARTP mutagenesis and damage-induced single-spore release technology, the thallus of Porphyra yezoensis on seed islands was segmented and mutated, solving the problem of low mutagenesis rate in existing Porphyra breeding, achieving efficient and targeted breeding results, and obtaining stable mutants.

CN122397618APending Publication Date: 2026-07-17SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SANYA INST OF OCEANOGRAPHY OCEAN UNIV OF CHINA
Filing Date
2026-05-29
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing seaweed breeding technologies, mutation breeding methods suffer from weak targeting, low mutation rate, and immature efficient mutation systems. There is a lack of efficient mutation breeding methods for seed island seaweed, making it difficult to quickly obtain mutants of the target traits.

Method used

The thallus of *Porphyra yezoensis* was segmented and mutated using ARTP mutagenesis combined with damage-induced single-spore release technology. After being cultured in the dark, the thallus was mechanically broken down to a specific cell size, mutants were screened, and the mutagenesis conditions were optimized to improve the mutation rate and stability.

Benefits of technology

It significantly shortens the mutagenesis cycle, increases the mutation rate and directionality, obtains stable mutants, and improves the efficiency and success rate of laver breeding, making it suitable for rapid breeding of seed island laver.

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Abstract

The application provides a mutagenesis breeding method based on seed island Porphyra and application thereof, and belongs to the technical field of breeding.The mutagenesis breeding method based on seed island Porphyra provided by the application comprises the following steps: selecting immature seed island Porphyra thalli for segmentation to obtain segmented leaves; the segmented leaves are subjected to ARTP mutagenesis, the leaves after mutagenesis are subjected to light-avoiding culture, and then mechanical crushing is performed to 20-40 cell sizes, and then the mutants are screened.The application combines the single spore dissemination technology induced by damage and the ARTP technology, uses the seed island Porphyra thalli as mutagenesis materials for mutagenesis, greatly shortens the mutagenesis period and reduces the workload, has the advantages of high efficiency, high mutation rate and good specificity, and can obtain stable mutants.
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