Biological macromolecule photocatalyst based on y-shaped DNA and its application in electrochemical nucleic acid biosensing detection of colorectal cancer
By using a biomacromolecule photocatalyst based on Y-type DNA and combining it with the ATRP reaction, the sensitivity and accuracy issues of miRNA-21 detection have been resolved, enabling efficient and low-cost detection for early colorectal cancer screening.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- WENZHOU KANGRUI BAIOU BIOTECHNOLOGY CO LTD
- Filing Date
- 2026-03-03
- Publication Date
- 2026-07-17
AI Technical Summary
Existing miRNA-21 detection methods suffer from low sensitivity, high cost, and the toxicity of metal catalysts can affect detection accuracy. Furthermore, residual ATRP catalysts can interfere with the current signal.
Using a biomacromolecule photocatalyst based on Y-type DNA, a Y-type DNA structure was synthesized through catalytic hairpin self-assembly technology. The DNA surface was then modified with methylene blue, and a ferrocene probe was modified on the electrode surface using the ATRP reaction, enabling ultrasensitive quantitative detection of miRNA-21.
It achieves high-precision, low-cost, and rapid electrochemical quantitative detection of miRNA-21, simplifies the construction process, and is suitable for early colorectal cancer screening.
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