A fluorescent probe for super-resolution imaging and preparation method and application thereof

By designing a novel fluorescent probe compound A-COO-LX, the problem of insufficient photostability in existing technologies was solved, achieving high photostability and long-term super-resolution imaging.

CN122404358APending Publication Date: 2026-07-17CHENGDU UNIV
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Patent Information

Application Number
CN202610600497.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-30
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing HaloTag fluorescent probes have insufficient photostability in super-resolution imaging, are prone to photobleaching, and are difficult to meet the requirements of long-term live cell imaging.

Method used

A novel fluorescent probe compound with the structure A-COO-LX was designed, where A is the rhodamine fluorophore, L is the ethylene glycol chain, and X is a halogen. It was prepared by a specific synthetic method to ensure efficient binding to the HaloTag protein and high photostability.

Benefits of technology

It achieves high fluorescence intensity under high-intensity laser irradiation, is suitable for long-term super-resolution imaging, reduces interference from autofluorescence in biological samples, and improves the photostability of imaging.

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Abstract

本发明涉及荧光探针技术领域，具体涉及一种用于超分辨成像的荧光探针及其制备方法和应用。该荧光探针具有如下通式结构：A‑COO‑L‑X，其中A为如下结构式所示的罗丹明荧光团：，L为‑(CH2CH2O)P‑(CH2)n‑，p为1～3的整数，n为2～12的整数，X为卤素原子。本发明制备的荧光探针与HaloTag融合蛋白的共定位系数达0.89，在STED超分辨成像中连续照射30分钟后荧光强度仅下降约22%，具有较好的靶向特异性和抗光漂白能力，可用于超分辨成像中的HaloTag融合蛋白标记。
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