A method and apparatus for isolating circulating tumor cells from leukocyte-depleted apheresis fluid
By modifying the microporous array filter membrane and designing a dual-layer filtration device, the problem of efficient separation and high-quality downstream analysis of circulating tumor cells in leukocyte collection fluid was solved, achieving high purity, high capture rate and cell viability preservation, suitable for clinical testing and laboratory research.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
- Filing Date
- 2026-03-26
- Publication Date
- 2026-07-17
AI Technical Summary
Existing technologies for separating circulating tumor cells from leukocyte apheresis fluid suffer from poor sorting characteristics, difficulty in supporting high-quality downstream cell culture and analysis, and in particular, an inability to balance cell heterogeneity and efficient capture, as well as being prone to clogging and complex to operate.
By modifying the microporous array filter membrane, including plasma activation, silanization, streptavidin modification, and biotinylated antibody coating, the pore size is expanded to 15 μm, and antibodies are densely modified on the pore walls. Combined with a dual-layer filtration device, physical size and affinity capture are achieved, clogging is avoided, and capture efficiency is improved.
It achieves high-purity and high-capture-rate isolation of circulating tumor cells, can process large volumes of leukocyte collection fluid, ensures cell viability and supports complex downstream analyses, and is suitable for clinical testing and laboratory research.
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