Application of a transport gene NtABCG6 in promoting genetic transformation efficiency of multiple species

By overexpressing or editing the NtABCG6 gene in plants, the transport of substances and homeostasis of the microenvironment are regulated, which solves the problems of low regeneration efficiency and poor stability of existing plant genetic transformation systems and achieves a highly efficient, stable and widely applicable regeneration promotion effect.

CN122405660APending Publication Date: 2026-07-17GUIZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUIZHOU UNIV
Filing Date
2026-05-29
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Existing plant genetic transformation systems suffer from low regeneration efficiency, long cycles, strong genotype dependence, and poor stability. Furthermore, traditional transcriptional regulation strategies have developmental toxicity and species limitations, making it difficult to achieve efficient, stable, and widely applicable regeneration promotion.

Method used

Using the NtABCG6 gene as a key module, we constructed a recombinant expression vector for overexpression or gene editing to regulate material transport and microenvironment homeostasis, promote callus and shoot differentiation, and improve regeneration efficiency and stability.

Benefits of technology

It significantly accelerated the time from Agrobacterium infection to obtaining positive seedlings, shortened the regeneration cycle, improved the efficiency of multi-species genetic transformation and gene editing, and did not cause obvious developmental defects or infertility.

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Abstract

The application belongs to the technical field of genetic engineering, and particularly relates to a transport gene NtABCG6 gene and application thereof in promoting genetic transformation efficiency of multiple species. The nucleotide sequence of the NtABCG6 gene is shown as SEQ ID No. 1. By expressing the NtABCG6 gene, the transition of callus to bud differentiation can be significantly accelerated, and the period from agrobacterium infection to obtaining a rooting positive plant is shortened. Stable regeneration promotion effects are exhibited in Nicotiana tabacum, tomato and potato, and a new strategy is provided for constructing a high-efficiency, stable and low-intervention plant genetic transformation system.
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