A method for creating a lodging-resistant wheat with improved plant type by using a CRISPR / Cas gene editing system
By targeting and knocking out the TaNPT1 protein in wheat using the CRISPR/Cas12i3-5M gene editing system, combined with T5E exonuclease treatment, the problem of poor lodging resistance in wheat was solved, and the stem structure and compressive strength were improved, thereby increasing wheat yield and quality.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- INSTITUTE OF CROP SCIENCE CHINESE ACADEMY OF AGRICULTURAL SCIENCES
- Filing Date
- 2025-01-16
- Publication Date
- 2026-07-17
AI Technical Summary
Existing wheat varieties have poor lodging resistance, leading to a decline in yield and quality. Furthermore, current gene editing technologies have low efficiency in wheat editing, making it difficult to effectively improve stem structure and compressive strength.
By using the CRISPR/Cas12i3-5M gene editing system to target and knock out the expression and activity of TaNPT1 protein in wheat, combined with T5E exonuclease treatment, the compressive strength and lodging resistance of the stems were improved. Efficient gene editing was achieved using CRISPR/Cas9 and CRISPR/T5E-Cas12i3-5M tools.
Without affecting wheat yield, it significantly improves wheat's lodging resistance, increases stem diameter and lignin content, enhances stem compressive strength, reduces tiller number, improves plant type, and increases yield and disease and pest resistance.
Smart Images
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