A stable and efficient nucleic acid amplification kit, a preparation method and application thereof
By adding low concentrations of urea, ethyl cellulose, and guar hydroxypropyltrimethylammonium chloride as protective agents, and sodium selenide and manganese chloride to the nucleic acid amplification kit to maintain enzyme activity, the problems of low nucleic acid amplification detection efficiency and poor stability were solved, achieving efficient and stable nucleic acid amplification results.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- ZHONGTUO BIOLOGICAL CO LTD
- Filing Date
- 2026-04-30
- Publication Date
- 2026-07-17
AI Technical Summary
Current nucleic acid amplification detection methods suffer from low efficiency, inability to undergo repeated freeze-thaw cycles, and short room temperature storage time, failing to meet the requirements of clinical testing and analysis.
By adding a low concentration of urea to the kit to enhance DNA polymerase activity, using ethyl cellulose and guar hydroxypropyltrimethylammonium chloride as protectants to safeguard the reagent components, adding sodium selenide and manganese chloride to maintain enzyme activity, and optimizing the buffer pH to 7.5-8.0, a stable and efficient nucleic acid amplification reagent was prepared.
This technology enables highly efficient amplification of reagents, improves the activity and stability of DNA polymerase, and maintains amplification performance under repeated freeze-thaw cycles and room temperature conditions, thus meeting clinical testing needs.
Smart Images
Figure SMS_1 
Figure SMS_2 
Figure SMS_3