A monkeypox virus rpa-sandwich lateral flow chromatographic detection kit and method based on bifunctional amplification products
By functionalizing RPA amplification primers, bifunctional amplification products carrying biotin-labeled and tagged nucleic acid sequences were formed, solving the problems of antibody dependence and equipment dependence of existing monkeypox virus detection methods. This enabled rapid and simple detection of monkeypox virus nucleic acid, which is suitable for field and primary healthcare institutions.
CN122405907APending Publication Date: 2026-07-17JINGZHOU FIRST PEOPLES HOSPITAL
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- JINGZHOU FIRST PEOPLES HOSPITAL
- Filing Date
- 2026-06-10
- Publication Date
- 2026-07-17
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Figure CN122405907A_ABST
Abstract
本发明公开了一种基于双功能扩增产物的猴痘病毒RPA‑侧流层析检测试剂盒及方法。所述试剂盒包括针对猴痘病毒F3L基因的RPA引物组、核酸功能化胶体金探针和侧流层析试纸条；其中，上游引物5'端通过间隔臂连接标签核酸序列，下游引物5'端带有生物素标记。经RPA扩增形成的双功能扩增产物可与核酸功能化胶体金探针特异性结合，并被检测线捕获显色，从而实现猴痘病毒核酸的快速可视化检测。本发明无需依赖抗体识别体系，具有体系设计简洁、操作简便、检测快速和结果直观等优点；在实施例条件下，检测下限可达50拷贝 / 反应，在猴痘病毒现场筛查、基层检测和应急监测中具有较好的应用前景。
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