Magnetic nano-enzyme microspheres as well as preparation method and application thereof

By preparing magnetic nanozyme microspheres Fe3O4@dSiO2/Pt, the sensitivity and stability issues of chloramphenicol and hexachlorobenzene detection were solved, realizing highly sensitive rapid detection of chloramphenicol by LFIA and hexachlorobenzene by ELISA, meeting the rapid detection needs of food and environmental safety monitoring.

CN122424876APending Publication Date: 2026-07-21HANGZHOU INST FOR ADVANCED STUDY UCAS
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Patent Information

Application Number
CN202610616774.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-07
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing rapid detection methods for chloramphenicol are not sensitive enough and have weak anti-interference capabilities, while quantitative detection methods for hexachlorobenzene are not efficient enough, making it difficult to meet the needs of rapid, sensitive and accurate food and environmental safety monitoring.

Method used

Using magnetic nanozyme microspheres Fe3O4@dSiO2/Pt as a carrier, a multifunctional composite microsphere probe was constructed by solvothermal method, Winsor III system and in-situ growth method, combined with magnetic enrichment and nanozyme catalysis, for rapid LFIA detection of chloramphenicol and ELISA detection of hexachlorobenzene.

Benefits of technology

The detection sensitivity and stability of chloramphenicol were significantly improved, with a qualitative detection cutoff of 0.1 ng/mL, a quantitative detection limit of 0.0011 ng/mL, and a hexachlorobenzene detection limit of 0.4 ng/mL, achieving detection results with high sensitivity and high stability.

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Abstract

The invention discloses a magnetic nano-enzyme microsphere as well as a preparation method and application thereof, and belongs to the cross technical fields of materials, medicines, rapid detection and the like. The magnetic nano-enzyme microsphere takes a Fe3O4 microsphere as an inner core, and platinum nanoparticles grow in situ after the surface of the Fe3O4 microsphere is coated with dendritic mesoporous silica. According to the magnetic nano-enzyme microspheres, magnetic response separation, high load content and enzyme catalysis amplification are utilized, so that the detection sensitivity and the signal intensity are greatly improved, and the magnetic nano-enzyme microspheres are suitable for high-sensitivity detection of trace chloramphenicol and hexachlorobenzene. The qualitative detection cut-off value of chloramphenicol is 0.1 ng / mL, the quantitative detection sensitivity of chloramphenicol is 0.0011 ng / mL, and the quantitative detection sensitivity of hexachlorobenzene is 0.4 ng / mL.
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