A method for the quantitative determination and characteristic chromatogram construction of twenty-five flavor coral pills by QAMS HPLC and its application

By employing gradient elution and wavelength switching technology in high-performance liquid chromatography, we have successfully achieved simultaneous quantitative detection and characteristic spectrum construction of multiple components in Twenty-Five Flavor Coral Pills. This solves the problems of high detection cost and low efficiency in existing technologies, and improves detection efficiency and quality control capabilities.

CN122430490APending Publication Date: 2026-07-21TIBENTAN RIKEZE ZANGNUO PHARMA CO LTD
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Patent Information

Application Number
CN202610570547.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-04-28
Publication Date
2026-07-21

AI Technical Summary

Technical Problem

Existing technologies are insufficient for simultaneously and efficiently detecting and controlling the quality of multiple active ingredients in Twenty-Five Flavor Coral Pills, and the detection costs are high and the instrument requirements are demanding, making it difficult to popularize.

Method used

High performance liquid chromatography (HPLC) with gradient elution and wavelength switching was used to simultaneously determine the contents of hydroxysaffron yellow A, mangiferin, crocin, linalool, crocin I, crocin II, and glycyrrhizic acid ammonium under a single elution condition, and characteristic chromatograms were constructed.

Benefits of technology

It enables simultaneous quantitative analysis of multiple components, reduces testing costs, improves testing efficiency, simplifies operating procedures, is suitable for promotion in routine laboratories, and significantly enhances testing efficiency and quality control capabilities.

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Abstract

The application discloses a method for quantitative determination and characteristic chromatogram construction of Tibetan medicine twenty-five coral pills by one-sample-multiple-assessment HPLC, and application thereof, and belongs to the technical field of detection and analysis. Chromatographic conditions of the application are as follows: mobile phase A (0.1% phosphoric acid water) - mobile phase B (acetonitrile) gradient elution. Through one injection, the same elution procedure, and switching of detection wavelengths in the process, the content determination of seven index components and the construction of characteristic chromatograms are successfully completed simultaneously. The method enables various effective components of traditional Chinese medicines with different properties and structures to be quantitatively analyzed through one injection in the same mobile phase system. Methodology verification shows that the obtained chromatogram has a smooth baseline and ideal peak separation. The outstanding advantages of the method are that no complicated extraction, concentration or evaporation steps are needed, and the method is simple, fast, accurate, reliable and good in reproducibility. Therefore, the method significantly improves the detection efficiency, reduces the operation cost, and embodies the concept of green analytical chemistry.
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