Anti-irritation composition of a composite antimicrobial agent, wet wipe preparation and use

Abstract

The application provides an anti-irritation composition of a composite antibacterial agent, a wet wipe solution and application, the anti-irritation composition comprising 0.05-0.15% of luteolin, 2-3% of a composite solubilizer and 0.05-0.15% of aminomethyl propanol; the anti-irritation composition of the composite antibacterial agent comprising 0.05-0.15% of luteolin, 1.0-3.0% of a composite solubilizer, 0.05-0.15% of aminomethyl propanol and 0.05-0.15% of a first antibacterial agent; and the solution comprising 10-50% of the above anti-irritation composition, 0.5-0.7% of a wet wipe solubilizer and 0.7-1.0% of a second antibacterial agent. The application increases the solubility of luteolin in pure water, improves the stability of the solution, significantly reduces the irritation of the chemical antibacterial agent on the basis of not reducing the content of the chemical antibacterial agent, retains the antibacterial activity of the chemical antibacterial agent and has good antibacterial effect.

Description

Technical Field

[0001] This invention relates to the field of medical and health preparation technology, and more specifically, to an anti-irritant composition of a compound antibacterial agent, a wet wipe formulation, and its application. Background Technology

[0002] Most commercially available antibacterial and preservative products use chemically synthesized antibacterial agents or preservatives. While these ingredients have some antibacterial effect, they generally have strong irritant properties and can easily cause skin discomfort. Long-term use can negatively impact skin health. Directly reducing the antibacterial agent content would reduce the product's irritation, but it would also decrease its antibacterial effect.

[0003] In research on anti-irritant ingredients derived from natural plants, honeysuckle, aloe vera, and ginseng are relatively common, and their applications are well-established. Existing literature on luteolin mainly focuses on its extraction process and its applications in antioxidant, antiviral, and pharmaceutical fields; research on the anti-irritant properties of luteolin remains relatively limited.

[0004] Luteolin has extremely low solubility in pure water, but its solubility increases under alkaline conditions. However, strongly alkaline conditions can easily irritate the skin. Moreover, as a plant extract, luteolin contains unstable active substances that have low solubility and are prone to precipitation, which can also lead to poor anti-irritant effects.

[0005] Therefore, developing an anti-irritant composition containing luteolin to reduce the skin irritation of chemical antibacterial agents while maintaining good antibacterial effects has significant practical implications and application prospects.

[0006] In view of this, the present invention is hereby proposed. Summary of the Invention

[0007] The purpose of this invention is to address the shortcomings of existing technologies. The primary objective of this invention is to provide a luteolin anti-irritant composition that increases the solubility of luteolin in pure water, improves solution stability, and solves the problem of unstable luteolin solution.

[0008] The second objective of this invention is to provide an anti-irritant composition, wet wipe formulation, and application of luteolin compound antibacterial agent. Without reducing the content of chemical antibacterial agents, luteolin can significantly reduce the irritation of chemical antibacterial agents while retaining their antibacterial activity, resulting in good antibacterial effects.

[0009] In order to achieve the above-mentioned objectives of the present invention, the following technical solution is adopted: A luteolin anti-irritant composition comprising 0.05-0.15 wt% luteolin, 2-3 wt% compound solubilizer, 0.05-0.15 wt% aminomethylpropanol, and the balance being pure water.

[0010] Furthermore, the composite solubilizer is at least two of 1,2-propanediol, PEG-40 hydrogenated castor oil, and glycerin.

[0011] Furthermore, the luteolin content is 0.05~0.1wt%.

[0012] Furthermore, the composite solubilizer is 1,2-propanediol and PEG-40 hydrogenated castor oil.

[0013] An anti-irritant composition of a luteolin complex antibacterial agent comprises 0.05-0.15 wt% luteolin, 1.0-3.0 wt% combined solubilizer, 0.05-0.15 wt% aminomethylpropanol, 0.05-0.15 wt% first antibacterial agent, and the balance being pure water.

[0014] Furthermore, the combined solubilizer is at least two of 1,2-propanediol, PEG-40 hydrogenated castor oil, and glycerin.

[0015] Furthermore, the first antibacterial agent is at least one of benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin.

[0016] Furthermore, the luteolin content is 0.05~0.1wt%.

[0017] Furthermore, the combined solubilizer is 1,2-propanediol and PEG-40 hydrogenated castor oil.

[0018] Furthermore, the first antibacterial agent is benzalkonium chloride.

[0019] A luteolin compound antibacterial agent for anti-irritant wipes formulation comprises 10-50 wt% of the above-mentioned luteolin anti-irritant composition, 0.5-0.7 wt% of wipes solubilizer, 0.7-1.0 wt% of a second antibacterial agent, and the balance being pure water; the luteolin content in the formulation is 0.005-0.05 wt%.

[0020] Furthermore, the wet wipe solubilizer is at least one of 1,2-propanediol and glycerol.

[0021] Furthermore, the second antibacterial agent is at least one of benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin.

[0022] Furthermore, the wet wipe solubilizer is 1,2-propanediol and glycerol.

[0023] Furthermore, the second antibacterial agent includes benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin.

[0024] Furthermore, the luteolin anti-irritant composition contains 0.1 wt% luteolin, and the luteolin content in the prepared solution is 0.01~0.05 wt%.

[0025] The application of the above-mentioned luteolin anti-irritant composition, or the above-mentioned luteolin complex antibacterial agent anti-irritant composition, or the above-mentioned luteolin complex antibacterial agent anti-irritant wipe preparation in the preparation of wipes.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. The luteolin-based anti-irritant composition, the luteolin-based compound antibacterial agent anti-irritant composition, and the wet wipe formulation of the present invention contain the natural active ingredient luteolin, are mild and non-irritating, and have multiple effects such as anti-inflammatory and antioxidant properties. The natural active ingredient luteolin in the luteolin-based compound antibacterial agent anti-irritant composition and wet wipe formulation of the present invention can significantly reduce the irritation of chemical antibacterial agents.

[0027] 2. Luteolin has extremely low solubility in pure water, but its solubility increases under alkaline conditions. However, strongly alkaline conditions can easily irritate the skin. The luteolin anti-irritant composition of this invention uses a system of 1,2-propanediol, PEG-40 hydrogenated castor oil, and aminomethylpropanol, which increases the solubility of luteolin in pure water, improves solution stability, and solves the problem of unstable luteolin solutions.

[0028] 3. The anti-irritant composition and wet wipe formulation of the luteolin compound antibacterial agent of the present invention can significantly reduce the irritation of chemical antibacterial agents without reducing the content of chemical antibacterial agents, while retaining the antibacterial activity of chemical antibacterial agents, and has a good antibacterial effect. Attached Figure Description

[0029] To more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0030] Figure 1 The results of the test on chicken embryos with the 0.1wt% aminomethylpropanol solution of the present invention are shown (the left side is a photo of the initial state of the chicken embryos at 0 min, and the right side is a photo of the state of the chicken embryos and the IS value 5 min after the corresponding component solution was added). Figure 2 The results of chicken embryo tests using 0.05wt% luteolin solution, 0.05wt% bisabolol solution, and 0.05wt% edelweiss solution are shown below (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 3 The results of chicken embryo tests using 0.1wt% luteolin solution, 0.1wt% bisabolol solution, and 0.1wt% edelweiss solution are shown below (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 4 The results of the chicken embryo test using the 0.1wt% benzalkonium chloride solution of this invention are shown (the left side is a photo of the chicken embryo in its initial state at 0 min, and the right side is a photo of the chicken embryo in its state and IS value 5 min after the addition of the corresponding component solution). Figure 5 The results of chicken embryo tests using 0.05wt% luteolin + 0.1wt% benzalkonium chloride solution, 0.05wt% bisabolol + 0.1wt% benzalkonium chloride solution, and 0.05wt% edelweiss + 0.1wt% benzalkonium chloride solution are shown in the image (left side is a photo of the chicken embryo at 0 min, right side is a photo of the chicken embryo at 5 min after adding the corresponding component solution and the IS value). Figure 6 The results of chicken embryo tests using 0.1wt% luteolin + 0.1wt% benzalkonium chloride solution, 0.1wt% bisabolol + 0.1wt% benzalkonium chloride solution, and 0.1wt% edelweiss + 0.1wt% benzalkonium chloride solution are shown in the image (left side is a photo of the chicken embryo at 0 min, right side is a photo of the chicken embryo at 5 min after adding the corresponding component solution and the IS value). Figure 7 The results of chicken embryo tests using 0.15wt% luteolin + 0.1wt% benzalkonium chloride solution, 0.15wt% bisabolol + 0.1wt% benzalkonium chloride solution, and 0.15wt% edelweiss + 0.1wt% benzalkonium chloride solution are shown in the image (left side is a photo of the chicken embryo at 0 min, right side is a photo of the chicken embryo at 5 min after adding the corresponding component solution and the IS value). Figure 8 The results of the test on chicken embryos in the wet wipes without luteolin of the present invention are shown (the left side is a photo of the initial state of the chicken embryo at 0 min, and the right side is a photo of the state of the chicken embryo and the IS value 5 min after adding the corresponding component solution). Figure 9 The results of chicken embryo testing for the wet wipes containing 0.01 wt% luteolin, 0.01 wt% bisabolol, and 0.01 wt% edelweiss (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and its IS value 5 min after adding the corresponding component solution). Figure 10The results of chicken embryo testing for the wet wipes containing 0.025 wt% luteolin, 0.025 wt% bisabolol, and 0.025 wt% edelweiss (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 11 The results of chicken embryo tests for the wet wipes containing 0.05 wt% luteolin, 0.05 wt% bisabolol, and 0.05 wt% edelweiss (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and its IS value 5 min after adding the corresponding component solution). Figure 12 This invention presents a composite graph of test results for chicken embryos using 0.1wt% benzalkonium chloride solution, 0.05wt% luteolin + 0.1wt% benzalkonium chloride solution, and 0.1wt% luteolin + 0.1wt% benzalkonium chloride solution (used to compare the effect of luteolin in reducing the irritation of benzalkonium chloride). Detailed Implementation

[0031] The technical solution of the present invention will be clearly and completely described below with reference to specific embodiments. However, those skilled in the art will understand that the embodiments described below are some embodiments of the present invention, but not all embodiments, and are only used to illustrate the present invention, and should not be regarded as limiting the scope of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall be followed. Where the manufacturers of reagents or instruments are not specified, they are all conventional products that can be purchased commercially.

[0032] Unless otherwise specified, all percentages of content or concentration in this invention and its accompanying drawings refer to mass fractions.

[0033] In this invention, bisabolol is a monocyclic sesquiterpene alcohol that is naturally found in various plant essential oils, especially in chamomile essential oil, where it is one of its core active ingredients. As a recognized "anti-allergy star," bisabolol can effectively inhibit the release of inflammatory mediators, significantly relieving skin redness, stinging, and itching, and has excellent efficacy in sensitive skin care and post-sun exposure repair.

[0034] In this invention, Edelweiss extract, also known as edelweiss extract, is a complex plant-based active compound. This plant natively grows in the extreme environment of the Alps at altitudes above 2000 meters, thus developing unique stress-relief active ingredients. Chlorogenic acid and its derivatives, as core components, are not only potent antioxidants and anti-inflammatory agents but also highly effective at scavenging free radicals. Simultaneously, this extract effectively downregulates the expression of inflammatory factors, significantly alleviates chronic inflammatory damage to the skin caused by environmental stress, and improves redness and sensitivity.

[0035] A luteolin anti-irritant composition comprising 0.05-0.15 wt% luteolin, 2-3 wt% compound solubilizer, 0.05-0.15 wt% aminomethylpropanol, and the balance being pure water.

[0036] Luteolin is a natural flavonoid compound widely found in vegetables (carrots, celery) and traditional Chinese medicines (honeysuckle, perilla, and crape myrtle), possessing various biological activities such as anti-inflammatory, antioxidant, and antibacterial properties. However, luteolin has extremely low solubility in pure water, while its solubility increases under alkaline conditions, but strongly alkaline conditions can easily irritate the skin.

[0037] Preferably, the composite solubilizer is at least two of 1,2-propanediol, PEG-40 hydrogenated castor oil, and glycerin; more preferably, the composite solubilizer is 1,2-propanediol and PEG-40 hydrogenated castor oil.

[0038] Preferred is aminomethylpropanol with low irritation, such as Figure 1 The photo shown is of a chicken embryo irritation test. The IS value of the chicken embryo containing 0.1% aminomethylpropanol was 1.86, which is considered mild irritation.

[0039] Preferably, the luteolin content is 0.05~0.1wt%, and the anti-irritant composition solution with a luteolin content of 0.15wt% produces precipitation after standing.

[0040] Preferably, the luteolin anti-irritant composition comprises 0.05-0.1 wt% luteolin, 1 wt% 1,2-propanediol, 2 wt% PEG-40 hydrogenated castor oil, 0.1 wt% aminomethylpropanol, and the balance being purified water. The luteolin anti-irritant composition of this invention uses 1,2-propanediol and PEG-40 hydrogenated castor oil for solubilization, and uses the low-irritant aminomethylpropanol to adjust the pH of the system, increasing the solubility of luteolin in purified water and solving the problems of luteolin precipitation and skin irritation. Figures 2-3The photograph shown illustrates the chicken embryo irritation test. The test results for 0.05 wt% and 0.1 wt% luteolin solutions showed no irritation. The irritation levels of different compositions were ranked as follows: 0.05 wt% luteolin solution < 0.05 wt% bisabolol solution < 0.05 wt% edelweiss solution; 0.1 wt% luteolin solution < 0.1 wt% bisabolol solution < 0.1 wt% edelweiss solution.

[0041] Preferably, the preparation method of the luteolin anti-irritant composition is as follows: 1,2-propanediol, PEG-40 hydrogenated castor oil, aminomethylpropanol and luteolin are added sequentially to pure water while stirring, the temperature is raised to 50°C, the mixture is dissolved and transparent, and the pH is adjusted to 6.5~7.0 with citric acid (10% aqueous solution).

[0042] An anti-irritant composition of a luteolin complex antibacterial agent comprises 0.05-0.15 wt% luteolin, 1.0-3.0 wt% combined solubilizer, 0.05-0.15 wt% aminomethylpropanol, 0.05-0.15 wt% first antibacterial agent, and the balance being pure water.

[0043] Preferably, the combined solubilizer is at least two of 1,2-propanediol, PEG-40 hydrogenated castor oil, and glycerin; more preferably, the combined solubilizer is 1,2-propanediol and PEG-40 hydrogenated castor oil.

[0044] Preferably, the first antibacterial agent is at least one of benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin; benzalkonium chloride is preferred.

[0045] Preferably, the anti-irritant composition of the luteolin complex antibacterial agent comprises 0.05~0.15wt% luteolin, 1wt% 1,2-propanediol, 1wt% PEG-40 hydrogenated castor oil, 0.1wt% aminomethylpropanol, 0.1wt% benzalkonium chloride, and the balance being pure water.

[0046] In the anti-irritant composition of the luteolin-based compound antibacterial agent of this invention, luteolin can significantly reduce the irritant properties of benzalkonium chloride, such as... Figures 4-7 The photographs shown depict chicken embryo irritation tests. The tests revealed that 0.05 wt% (IS value 5.20), 0.1 wt% (IS value 4.39), and 0.15 wt% (IS value 3.17) of luteolin reduced the irritation of 0.1 wt% benzalkonium chloride (IS value 14.1) from strong to low-to-medium irritation. The reduction in irritation of 0.1 wt% benzalkonium chloride by 0.05 wt%, 0.1 wt%, and 0.15 wt% bisabolol, and by 0.05 wt%, 0.1 wt%, and 0.15 wt% edelweiss, was less than the reduction by luteolin at the same concentration.

[0047] Preferably, such as Figure 12 Comparing the effects of luteolin in reducing the irritation of benzalkonium chloride, it can be seen that the anti-irritant composition solution of the compound antibacterial agent with a luteolin content of 0.05~0.1wt% and 0.15wt% luteolin content produced precipitation after standing.

[0048] Preferably, the anti-irritant composition of the luteolin compound antibacterial agent is prepared by adding 1,2-propanediol, benzalkonium chloride, PEG-40 hydrogenated castor oil, aminomethylpropanol and luteolin sequentially to pure water while stirring, heating to 50°C, dissolving and making transparent, and adjusting the pH to 6.5~7.0 with citric acid (10% aqueous solution).

[0049] A luteolin compound antibacterial agent for anti-irritant wipes formulation comprises 10-50 wt% of the above-mentioned luteolin anti-irritant composition, 0.5-0.7 wt% of wipe solubilizer, 0.7-1.0 wt% of second antibacterial agent, and the balance being pure water; the luteolin content in the formulation is 0.005-0.05 wt%.

[0050] Preferably, the wet wipe solubilizer is at least one of 1,2-propanediol and glycerin; more preferably, 1,2-propanediol and glycerin.

[0051] Preferably, the second antibacterial agent is at least one of benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin.

[0052] Preferably, the anti-irritant wipe formulation of the luteolin compound antibacterial agent comprises: 10-50 wt% of the above-mentioned luteolin anti-irritant composition (0.1 wt% luteolin), 0.3 wt% of 1,2-propanediol, 0.2 wt% of benzalkonium chloride, 0.5 wt% of chlorhexidine gluconate, 0.15 wt% of chlorphenesin, and the balance being pure water; the luteolin content in the formulation is 0.01-0.05 wt%.

[0053] In the anti-irritant wipes formulation of the luteolin compound antibacterial agent of this invention, luteolin can significantly reduce the irritation of benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin. Figures 8-11 The photo shows a chicken embryo irritation test. The test revealed that adding 0.01 wt% (IS value 6.41), 0.025 wt% (IS value 5.34), and 0.05 wt% (IS value 5.02) of luteolin reduced the irritation of the wipes from strong irritation (IS value of wipes without luteolin 14.86) to low-to-medium irritation. The reduction in irritation of wipes with 0.01 wt%, 0.025 wt%, and 0.05 wt% bisabolol, and with 0.01 wt%, 0.025 wt%, and 0.05 wt% edelweiss, was less than the reduction in irritation with the same amount of luteolin.

[0054] Preferably, the preparation method of the anti-irritant wipe solution of the luteolin compound antibacterial agent is as follows: glycerin, 1,2-propanediol, chlorphenesin, benzalkonium chloride, chlorhexidine gluconate, and the above-mentioned luteolin anti-irritant composition are added sequentially to pure water, and the pH is adjusted to 6.0-7.0 with citric acid (10% aqueous solution).

[0055] The application of the above-mentioned luteolin anti-irritant composition, or the above-mentioned luteolin complex antibacterial agent anti-irritant composition, or the above-mentioned luteolin complex antibacterial agent anti-irritant wipe preparation in the preparation of wipes.

[0056] Example 1 A method for preparing a luteolin anti-irritant composition is as follows: 1,2-propanediol, PEG-40 hydrogenated castor oil, aminomethylpropanol and luteolin are added sequentially to pure water while stirring. The mixture is heated to 50°C until it dissolves and becomes transparent. The pH is then adjusted to 6.5~7.0 with citric acid (10% aqueous solution).

[0057] The specific reagent dosages are shown in Table 1: Table 1. Reagent addition amount in Example 1

[0058] The luteolin anti-irritant composition prepared in Example 1 was observed for precipitation, and a chicken embryo test was performed. The results were as follows: a 0.1 wt% luteolin solution was clear, and the chicken embryo test result was IS 0.79 (no irritation). Figure 3 As shown.

[0059] Example 2 The difference between this embodiment and embodiment 1 is that the specific amount of reagent added is shown in Table 2, while the rest is the same as in embodiment 1.

[0060] Table 2. Reagent addition amount in Example 2

[0061] The luteolin anti-irritant composition prepared in Example 2 was observed for precipitation, and a chicken embryo test was performed. The results were as follows: a 0.05 wt% luteolin solution was clear, and the chicken embryo test result was IS 0.98 (no irritation). Figure 2 As shown.

[0062] Example 3 The difference between this embodiment and embodiment 1 is that the specific amount of reagents added is shown in Table 3, while the rest is the same as in embodiment 1.

[0063] Table 3. Reagent addition amount in Example 3

[0064] The luteolin anti-irritant composition prepared in Example 3 was observed for precipitation, and a chicken embryo test was performed. The results showed that a 0.15 wt% luteolin solution produced precipitation after standing.

[0065] Example 4 A method for preparing an anti-irritant composition of a luteolin compound antibacterial agent is as follows: 1,2-propanediol, benzalkonium chloride, PEG-40 hydrogenated castor oil, aminomethylpropanol, and luteolin are added sequentially to pure water while stirring. The mixture is heated to 50°C until it dissolves and becomes transparent. The pH is then adjusted to 6.5-7.0 with citric acid (10% aqueous solution).

[0066] The specific reagent addition amounts are shown in Table 4, and the rest are the same as in Example 1.

[0067] Table 4. Reagent addition amount in Example 4

[0068] The anti-irritant composition of the luteolin compound antibacterial agent prepared in Example 4 was observed for precipitation, and a chicken embryo test was performed. The experimental results were as follows: a 0.1 wt% luteolin + 0.1 wt% benzalkonium chloride solution was clear, and the chicken embryo test result was IS 4.39 (moderate irritation). Figure 6 As shown.

[0069] Example 5 The difference between this embodiment and embodiment 4 is that the specific amount of reagent added is shown in Table 5, while the rest is the same as in embodiment 4.

[0070] Table 5. Reagent addition amount in Example 5

[0071] The anti-irritant composition of the luteolin compound antibacterial agent prepared in Example 5 was observed for precipitation, and a chicken embryo test was performed. The experimental results were as follows: 0.05wt% luteolin + 0.1wt% benzalkonium chloride solution was clear, and the chicken embryo test result was IS5.20 (moderate irritation). Figure 5 As shown.

[0072] Example 6 The difference between this embodiment and embodiment 4 is that the specific amount of reagent added is shown in Table 6, while the rest is the same as in embodiment 4.

[0073] Table 6. Reagent addition amount in Example 6

[0074] The anti-irritant composition of the luteolin compound antibacterial agent prepared in Example 6 was observed for precipitation, and a chicken embryo test was performed. The results showed that a precipitate formed after standing in a 0.15 wt% luteolin + 0.1 wt% benzalkonium chloride solution. The chicken embryo test result was IS 3.17 (mild irritation). Figure 7 As shown.

[0075] Example 7 A solution for anti-irritant wipes containing a luteolin compound antibacterial agent is prepared using the luteolin anti-irritant composition prepared in Example 1. Glycerin, 1,2-propanediol, chlorphenesin, benzalkonium chloride, chlorhexidine gluconate, and the luteolin anti-irritant composition prepared in Example 1 are added sequentially to pure water while stirring until dissolved and transparent. The pH is then adjusted to 6.0-7.0 using citric acid (10% aqueous solution).

[0076] The specific reagent addition amounts are shown in Table 7, and the rest are the same as in Example 1.

[0077] Table 7. Reagent addition amount in Example 7

[0078] Non-woven fabric was selected as the membrane substrate. The non-woven fabric was cut into 200mm*180mm pieces. Ten pieces of non-woven fabric were weighed, and the solution was evenly poured onto the non-woven fabric, ensuring the weight of the solution was three times the weight of the non-woven fabric. The membranes were then packaged and a chicken embryo irritation test was performed. The experimental results were: 0.05wt% luteolin-containing wipes with chicken embryos showed an IS 5.02 (moderate irritation). Figure 11 As shown.

[0079] Example 8 The difference between this embodiment and embodiment 7 is that the specific amount of reagent added is shown in Table 8, while the rest is the same as in embodiment 7.

[0080] Table 8. Reagent addition amount in Example 8

[0081] Non-woven fabric was selected as the membrane substrate. The non-woven fabric was cut into 200mm*180mm pieces. Ten pieces of non-woven fabric were weighed, and the solution was evenly poured onto the non-woven fabric, ensuring the weight of the solution was three times the weight of the non-woven fabric. The mixture was then packaged and placed in a bag for a chicken embryo irritation test. The experimental results were: 0.01wt% luteolin-containing wipes with chicken embryos showed an IS 6.41 (moderate irritation). Figure 9 As shown.

[0082] Example 9 The difference between this embodiment and embodiment 7 is that the specific amount of reagent added is shown in Table 9, while the rest is the same as in embodiment 7.

[0083] Table 9. Reagent addition amount in Example 9

[0084] Non-woven fabric was selected as the substrate for the membrane. The non-woven fabric was cut into 200mm*180mm pieces. Ten pieces of non-woven fabric were weighed, and the solution was evenly distributed onto the non-woven fabric, ensuring the weight of the solution was three times the weight of the non-woven fabric. The membranes were then packaged and a chicken embryo irritation test was performed. The experimental results were: 0.025wt% luteolin-containing wipes with chicken embryos showed an IS 5.34 (moderate irritation). Figure 10 As shown.

[0085] Comparative Example 1 The difference between this comparative example and Example 1 is that 1,2-propanediol is removed, and the specific reagent addition amounts are shown in Table 10. Otherwise, it is the same as Example 1.

[0086] Table 10. Reagent addition amount in Comparative Example 1

[0087] Observe the stability of the solution.

[0088] Experimental results: The solution was unstable and a precipitate was formed.

[0089] Comparative Example 2 The difference between this comparative example and Example 1 is that PEG-40 hydrogenated castor oil is removed, and the specific reagent addition amounts are shown in Table 11. Otherwise, it is the same as Example 1.

[0090] Table 11 Reagent addition amount in Comparative Example 2

[0091] Observe the stability of the solution.

[0092] Experimental results: The solution was unstable and a precipitate was formed.

[0093] Comparative Example 3 The difference between this comparative example and Example 1 is that aminomethylpropanol is removed, and the specific reagent addition amounts are shown in Table 12. Otherwise, it is the same as Example 1.

[0094] Table 12 Reagent addition amount in Comparative Example 3

[0095] Observe the stability of the solution.

[0096] Experimental results: The solution was unstable and a precipitate was formed.

[0097] Comparative Example 4 The difference between this comparative example and Example 4 is that it does not contain luteolin, and the specific reagent addition amounts are shown in Table 13. The rest is the same as Example 4.

[0098] Table 13 Reagent addition amount in Comparative Example 4

[0099] Comparative Example 5 The difference between this comparative example and Example 7 is that it does not contain luteolin, and the specific reagent addition amounts are shown in Table 14. The rest is the same as in Example 7.

[0100] Table 14. Reagent addition amount in Comparative Example 5

[0101] Non-woven fabric was selected as the substrate for the membrane. The non-woven fabric was cut into 200mm*180mm blocks. Ten pieces of non-woven fabric were weighed, and the solution was evenly placed on the non-woven fabric. The weight of the solution was controlled to be three times the weight of the non-woven fabric. The solution was then sealed in a bag and a chicken embryo irritation test was conducted.

[0102] The experimental results showed that the IS value for irritation in chicken embryos was 14.86 (strong irritation). Figure 8 As shown.

[0103] Comparative Example 6 aminomethylpropanol chicken embryo irritation test Prepare a 0.1% aminomethylpropanol solution, adjust the pH to 6.5-7.0 with citric acid (10% aqueous solution), and perform a chicken embryo irritation test. The result is: IS 1.86 (mild irritation). Figure 1 As shown.

[0104] Comparative Example 7 Benzalkonium chloride chicken embryo irritation test A 0.1% benzalkonium chloride solution was prepared, and the pH was measured to be 6.72. Results for chicken embryos: IS 14.1 (strong irritant). Figure 4 As shown.

[0105] Comparative Example 8 In Example 2, the luteolin in the 0.05 wt% luteolin solution was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 2 As shown; Comparative Example 9 In Example 1, the luteolin in the 0.1 wt% luteolin solution was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 3 As shown; Comparative Example 10 In Example 5, the luteolin in the 0.05wt% luteolin + 0.1wt% benzalkonium chloride solution was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 5 As shown; Comparative Example 11 In Example 4, the luteolin in the 0.1 wt% luteolin + 0.1 wt% benzalkonium chloride solution was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 6 As shown; Comparative Example 12 In Example 6, the luteolin in the 0.15wt% luteolin + 0.1wt% benzalkonium chloride solution was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 7 As shown; Comparative Example 13 In Example 8, the luteolin in the 0.01 wt% luteolin-containing wipes was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 9 As shown; Comparative Example 14 In Example 9, the luteolin in the 0.025 wt% luteolin-containing wipes was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 10 As shown; Comparative Example 15 In Example 7, the luteolin in the 0.05 wt% luteolin-containing wipes was replaced with the same concentration of bisabolol and edelweiss for chicken embryo testing (the left side shows the initial state of the chicken embryo at 0 min, and the right side shows the state of the chicken embryo and IS value 5 min after adding the corresponding component solution). Figure 11 As shown.

[0106] II. Antibacterial performance test: The antibacterial properties of compositions or wipe formulations prepared by replacing luteolin with the same concentration of bisabolol and edelweiss in Examples 1-9, Comparative Examples 4-5, and Comparative Examples 10-15 were tested to assess their inhibitory effects on Staphylococcus aureus and Escherichia coli. The inhibition rates against Staphylococcus aureus and Escherichia coli were tested according to GB15979-2024 "Hygienic Requirements for Disposable Sanitary Products". The experimental conditions used the composition or wipe formulation as the sample solution, and the inhibition time was 5 minutes. The specific experimental steps are as follows: Bacterial species names and codes: Staphylococcus aureus (ATCC 6538), Escherichia coli (ATCC25922) Experimental preparation: PBS: pH 7.2 phosphate buffer 4.36g, deionized water 400mL, sterilization conditions: 121℃, 15min.

[0107] Bacterial culture medium: 14.85g nutrient agar, 2400mL deionized water, sterilization conditions: 121℃, 15min.

[0108] Preparation of Staphylococcus aureus culture: Using an inoculation loop, pick an appropriate amount of Staphylococcus aureus culture and dilute it with sterile PBS to approximately 1.0 × 10⁻⁶. 5 CFU / mL - 9.0 × 10 5 The prepared bacterial suspension (CFU / mL) should be used within 2 hours or stored at 2℃-8℃ for no more than 24 hours.

[0109] Preparation of Escherichia coli culture: A suitable amount of bacteria was picked from the E. coli culture using an inoculation loop and diluted with sterile PBS to approximately 1.0 × 10⁻⁶. 5 CFU / mL - 9.0 × 10 5 The prepared bacterial suspension (CFU / mL) should be used within 2 hours or stored at 2℃-8℃ for no more than 24 hours.

[0110] Experimental steps: Take 4.5 mL of the test composition or wet wipe solution and mix it thoroughly with 0.5 mL of Staphylococcus aureus and 0.5 mL of Escherichia coli, respectively. After standing for 5 min, dilute appropriately and select 2-3 dilutions to inoculate onto bacterial culture medium. At the same time, set up control groups and inoculate Staphylococcus aureus and Escherichia coli separately onto bacterial culture medium. Incubate the experimental group and control group at 30℃ for 24 h, and then count the colonies separately. Substitute into the formula: inhibition rate (%) = [(AB) / A] × 100%, where A is the average number of viable bacteria per milliliter in the control group (in CFU / mL) and B is the average number of viable bacteria per milliliter in the experimental group (in CFU / mL) to calculate the inhibition rate.

[0111] The experimental results are shown in Table 15: Table 15. Irritation test results and antibacterial performance test results of compositions or wipe solutions prepared by replacing luteolin with the same concentration of bisabolol and edelweiss in Examples 1-9, Comparative Examples 4-5, and Comparative Examples 10-15.

[0112] Comparing the irritation test results of compositions or wipe solutions prepared by replacing luteolin with the same concentration of bisabolol and edelweiss in Examples 1-9, Comparative Examples 4-5, and Comparative Examples 10-15, it was found that luteolin, compared with the same concentration of bisabolol and edelweiss, can more significantly reduce the irritation of chemical antibacterial agents (benzalkonium chloride) in the composition and the irritation of chemical antibacterial agents (benzalkonium chloride, chlorhexidine gluconate, chlorphenesin) in the wipes, and can effectively retain the antibacterial activity of chemical antibacterial agents, resulting in good antibacterial effect.

[0113] Examples 1-3 exhibit certain antibacterial properties due to the multiple biological activities of luteolin, including anti-inflammatory, antioxidant, and antibacterial effects. Examples 4-6 show improved antibacterial properties due to the addition of the chemical antibacterial agent benzalkonium chloride. Compared to Comparative Example 4, Examples 4-6 show no decrease in antibacterial properties, indicating that the antibacterial activity of the chemical antibacterial agent (benzalkonium chloride) was retained. Compared to Comparative Example 5, Examples 7-9 show essentially the same antibacterial properties, indicating that the antibacterial activity of the chemical antibacterial agents (benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin) was retained. Compared to compositions where luteolin was replaced with the same concentration of bisabolol and edelweiss, Examples 4-6 retain more of the antibacterial activity of the chemical antibacterial agent (benzalkonium chloride). Compared to wipe formulations where luteolin was replaced with the same concentration of bisabolol and edelweiss, Examples 7-9 retain more of the antibacterial activity of the chemical antibacterial agents (benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin).

Claims

1. An anti-irritant composition of a luteolin-based compound antibacterial agent, characterized in that, It includes 0.05-0.1 wt% luteolin, 1.0-3.0 wt% combined solubilizer, 0.05-0.15 wt% aminomethylpropanol, 0.05-0.15 wt% primary antibacterial agent, and the balance being pure water; The combined solubilizer is 1,2-propanediol and PEG-40 hydrogenated castor oil; The first antibacterial agent is benzalkonium chloride.

2. A solution for anti-irritant wipes containing a luteolin-based compound antibacterial agent, characterized in that, The solution comprises 10-50 wt% luteolin anti-irritant composition, 0.5-0.7 wt% wet wipe solubilizer, 0.7-1.0 wt% secondary antibacterial agent, and the balance being pure water; the luteolin content in this solution is 0.005-0.05 wt%. The luteolin anti-irritant composition comprises 0.05-0.1 wt% luteolin, 2-3 wt% compound solubilizer, 0.05-0.15 wt% aminomethylpropanol, and the balance being pure water; The composite solubilizer is 1,2-propanediol and PEG-40 hydrogenated castor oil; The second antibacterial agent includes benzalkonium chloride, chlorhexidine gluconate, and chlorphenesin.

3. The anti-irritant wipe formulation of the luteolin compound antibacterial agent according to claim 2, characterized in that, The wet wipe solubilizer is at least one of 1,2-propanediol and glycerol.

4. The anti-irritant wipe formulation of the luteolin compound antibacterial agent according to claim 3, characterized in that, Includes at least one of the following technical features: (1) The wet wipe solubilizer is 1,2-propanediol and glycerin; (2) The luteolin anti-irritant composition contains 0.1 wt% luteolin, and the luteolin content in the solution is 0.01~0.05 wt%.

5. The anti-irritant composition of the luteolin compound antibacterial agent as described in claim 1, or the application of the anti-irritant wet wipe solution of the luteolin compound antibacterial agent as described in any one of claims 2 to 4 in the preparation of wet wipes.

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