Use of a novel 3-methyl-2-oxobutyrate hydroxymethyltransferase

By expressing PanB enzymes of *Clerodendrum chalcogenide* in pantothenic acid-producing or pantothenic acid-releasing microorganisms, the high cost and pollution problems of D-pantothenic acid synthesis have been solved, enabling low-cost, high-efficiency, and environmentally friendly production of vitamin B5.

CN122445601APending Publication Date: 2026-07-24TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Filing Date
2025-01-23
Publication Date
2026-07-24

AI Technical Summary

Technical Problem

Existing technologies for preparing vitamin B5 suffer from problems such as high cost, heavy pollution, and expensive raw materials. In particular, the synthesis of D-pantolytic acid has become a key bottleneck in the biosynthesis of vitamin B5.

Method used

By genetically modifying pantothenic acid or pantothenic acid-producing microorganisms to express 3-methyl-2-oxobutyrate hydroxymethyltransferase (PanB) from *Cladosporium variegatum*, and culturing these microorganisms under appropriate conditions to produce pantothenic acid or pantothenic acid.

Benefits of technology

This has enabled the low-cost, high-chirality, and low-pollution synthesis of D-pantolytic acid, promoting the green and environmentally friendly biosynthesis of vitamin B5 and facilitating its industrialization.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the use of PanB from Modestobacter versicolor in the production of pantothenate or pantoic acid and in the preparation of microorganisms producing pantothenate or pantoic acid and methods of producing pantothenate or pantoic acid using the same.
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Citation Information

Patent Citations

  • Microorganism capable of producing pantoic acid as well as construction method and application of microorganism

    CN115109736A

  • A pantoic acid-producing microorganism and its construction method and application

    CN115109736B

  • Microorganism for producing pantoic acid, and construction method therefor and application thereof

    WO2022198846A1