Expression constructs and methods for genetically engineering methylotrophic yeasts
By introducing recombinant nucleic acid molecules into methyltrophic yeast, efficient expression of peptides was achieved under methanol-free induction conditions, solving the problem of insufficient expression levels in existing technologies and increasing the recombinant peptide yield.
CN122503237APending Publication Date: 2026-08-04IMPOSSIBLE FOODS INC
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Patent Information
- Application Number
- CN202610337257.9
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Priority Date
- 2016-03-25
- Filing Date
- 2016-05-11
- Publication Date
- 2026-08-04
AI Technical Summary
Technical Problem
Existing technologies struggle to efficiently express peptides in methyltrophic yeasts, especially in the absence of methanol inducers, and traditional methods suffer from insufficient expression levels.
Method used
By introducing recombinant nucleic acid molecules into methyltrophic yeast, a positive feedback loop is formed by operably linking exogenous nucleic acids encoding transcription activators with methanol-inducible promoter elements to achieve efficient peptide expression.
Benefits of technology
It significantly improved the recombinant yield of peptides, especially under methanol-free conditions, enhanced the expression of transcription activators and target peptides, and solved the problem of insufficient expression levels.
✦ Generated by Eureka AI based on patent content.
Abstract
The present application relates to expression constructs and methods for genetically engineering methylotrophic yeasts. The present invention provides methods and materials for genetically engineering methylotrophic yeasts.
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