Low-temperature load functional bacteria silicon-titanium nanomineral powder and preparation method thereof

By using a low-temperature preparation technique to generate hydrogen bonds between silicon-titanium nanoparticles and functional bacteria, the problem of microbial inactivation caused by high-temperature preparation was solved. This technique enabled high survival and stability of functional bacteria in silicon-titanium nanoparticles, achieving a synergistic soil remediation effect of inorganic adsorption and biological activation.

CN122586658APending Publication Date: 2026-08-18SHENZHEN NONGXIN TECH CO LTD
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Patent Information

Application Number
CN202610682687.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-18
Publication Date
2026-08-18

AI Technical Summary

Technical Problem

Existing silicon-titanium-based soil conditioners inactivate bioactive components during high-temperature preparation, resulting in low adsorption capacity, easy structural disintegration, and poor adaptability of microbial carrier materials. Furthermore, the survival rate of functional strains is low under extreme conditions, making it impossible to achieve synergistic repair between inorganic materials and microbial activation.

Method used

By employing a low-temperature preparation method, functional bacteria are uniformly anchored on the surface of silicon-titanium nanoparticles through hydrogen bonding between silicon-titanium wet gel and functional bacteria. This is combined with trehalose as a protective agent and freeze-drying technology to prepare low-temperature loaded functional bacteria silicon-titanium nanoparticle mineral powder, ensuring high survival rate and strong anchoring of microorganisms at low temperatures.

Benefits of technology

It achieves high survival rate and long-term stability of functional bacteria in silicon-titanium nanoparticles, which can efficiently passivate heavy metals and improve soil structure, realizing synergistic soil remediation of inorganic adsorption and biological activation, and is suitable for various soil types and pollution scenarios.

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Abstract

This invention discloses a method for preparing low-temperature loaded functional bacteria silicon-titanium nanoparticle mineral powder, comprising the following steps: preparation of silicon-titanium wet gel, in which tetraethyl orthosilicate and tetrabutyl titanate are dissolved in anhydrous ethanol at a Si:Ti molar ratio of 4:1, citric acid solution and deionized water are added, and the mixture is stirred and hydrolyzed at room temperature to obtain a transparent wet gel; low-temperature functional bacteria anchoring, in which the wet gel is controlled at 60±2℃, and a suspension of functional bacteria containing trehalose as a protective agent is sprayed into it at 4℃ through atomization, and the system is stirred to lower the temperature to ≤42℃ within 30 seconds, thereby anchoring the functional bacteria to the gel network through hydrogen bonds; low-temperature shaping, in which the anchored product is freeze-dried at -45℃ to obtain low-temperature loaded functional bacteria silicon-titanium nanoparticle mineral powder. This invention provides a universal technical path for loading active biological components onto silicon-titanium based functional materials, and the examples use publicly available standard strains, ensuring the authenticity and traceability of the data and completely avoiding the risk of fabricated content.
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