Sample application identification device for yeast cell culture

Abstract

The utility model belongs to the technical field of yeast cell culture, and particularly relates to a sample application identification device for yeast cell culture, which comprises a culture dish and a sample application identifier, each of the culture dish and the sample application identifier comprises a side wall and a bottom wall, the bottom wall is fixedly connected to the lower side of the side wall, and the inner diameter of the culture dish is larger than the outer diameter of the sample application identifier. The bottom wall of the sample application identifier is made of a transparent material, a plurality of sample application identification point positions are arranged on the upper side of the bottom wall of the culture dish and are in a rectangular array, the sample application identification device further comprises a plurality of sample application identification units, and each sample application identification unit comprises a use state and a storage state. The sample application identification unit can be installed on any sample application identification point position when in a use state, and the sample application identification unit is separated from the sample application identification point position when in a storage state. According to the utility model, the phenomena of multiple points and wrong points in the sample application process of diluted samples of yeast cultures by operators can be reduced.

Description

Technical Field

[0001] The utility model belongs to the technical field of yeast cell culture, and particularly relates to a spotting marking device for yeast cell culture. Background Art

[0002] When culturing yeast cells, the cultured cells need to be diluted in gradient concentrations and spotted onto screening culture media to detect their growth status;

[0003] In order to reduce the phenomenon of uneven rows and columns when spotting the diluted samples of yeast culture, it is necessary to use a spotting marking device for assistance. For example, the Chinese patent with the authorization announcement number CN206244773U discloses a yeast cell culture concentration gradient dilution spotting marker, which is highly practical, easy to operate, time-saving and labor-saving, and has high repeatability; the spotting is stable, the sample points are evenly distributed, and the standardization of the experimental results and work efficiency are improved. The utility model is mainly used to prepare a yeast cell culture concentration gradient dilution spotting marker;

[0004] The above patent sets several spotting circles on the spotting marker, and then selects multiple spotting circles as identification areas, and spots the diluted samples of yeast culture in the identification areas. However, due to the large number of spotting circles, the diluted samples of yeast culture can easily be spotted multiple times or mistakenly on the remaining spotting circles. Utility Model Content

[0005] The utility model aims to provide a spotting identification device for yeast cell culture, which can reduce the phenomenon of multiple spots and wrong spots in the process of operators spotting diluted samples of yeast culture.

[0006] The technical solutions adopted in this utility are as follows:

[0007] A spot marking device for yeast cell culture, comprising a culture dish and a spot marking device, wherein the culture dish and the spot marking device both comprise a side wall and a bottom wall, wherein the bottom wall is fixedly connected to the lower side of the side wall, and the inner diameter of the culture dish is larger than the outer diameter of the spot marking device, and the bottom wall of the spot marking device is made of a transparent material, and a plurality of spot marking points are arranged on the upper side of the bottom wall of the culture dish, wherein the plurality of spot marking points are in a rectangular array;

[0008] The spot marking device also includes a plurality of spot marking units, wherein the spot marking units include a use state and a storage state. When the spot marking units are in the use state, they can be installed at any spot marking point, and when they are in the storage state, they are separated from the spot marking points.

[0009] Furthermore, the spot identification point includes a clamping ring fixedly connected to the bottom wall of the culture dish, and the spot identification unit includes an identification body, a clamping block is fixedly connected to the lower part of the identification body, and the clamping block can be clamped inside the clamping ring.

[0010] Furthermore, a second magnetic component is fixedly connected to the lower side of the inner part of the clamping ring, and a first magnetic component that is magnetically attracted to the second magnetic component is fixedly connected to the lower side of the clamping block.

[0011] Furthermore, an elastic gasket is fixedly connected to the upper side of the clamping ring.

[0012] Furthermore, the identification body includes an identification plate fixedly connected to the upper side of the card block, and the upper side of the identification plate is provided with a dotted cross line and a card block located outside the dotted cross line.

[0013] Furthermore, two adjusting rods are installed on the side wall of the culture dish, and a clamping piece is installed on one end of the adjusting rod located inside the culture dish.

[0014] The technical effects achieved by this utility model are:

[0015] The present invention is a practical spot marking device for yeast cell culture. According to the needs of users, a plurality of spot marking units 4 are assembled on corresponding spot marking points 3, and then a spot marking device 2 is placed inside a culture dish 1, and the bottom wall of the spot marking device 2 and the upper side of the spot marking unit 4 are abutted against each other, so that the spot marking unit 4 is used as a marking area at the bottom wall of the spot marking device 2. An operator drips a diluted sample of yeast culture on the marking area at the bottom wall of the spot marking device 2, and through the operation of the spot marking unit, the number of the spot marking units and the points to be spotted by the operator are made the same, thereby reducing the phenomenon of multiple spots and wrong spots in the process of the operator spotting the diluted sample of yeast culture. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of this utility model;

[0017] Figure 2 It is a structural schematic diagram of the practical culture dish;

[0018] Figure 3 It is a structural schematic diagram of the practical spot marking point and spot marking unit;

[0019] Figure 4 It is a cut-away side view of the practical spot marking point and spot marking unit.

[0020] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0021] 1. Culture dish; 2. Spot marker; 3. Spot marker point; 4. Spot marker unit; 5. Adjustment rod; 6. Clamping piece; 7. Marking plate; 8. Spot marker ring; 9. Spot crosshair; 10. Clamping block; 11. First magnetic part; 12. Clamping ring; 13. Second magnetic part; 14. Elastic gasket. DETAILED DESCRIPTION

[0022] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is specifically described below in conjunction with the embodiments. It should be understood that the following text is only used to describe one or several specific implementations of the present invention, and does not strictly limit the scope of protection specifically requested by the present invention.

[0023] like Figure 1 As shown, a spotting marker device for yeast cell culture includes a culture dish 1 and a spotting marker 2, both of which are containers with an opening on the upper side, that is, the culture dish 1 and the spotting marker 2 both include a side wall and a bottom wall, the bottom wall is fixedly connected to the lower side of the side wall, and the inner diameter of the culture dish 1 is larger than the outer diameter of the spotting marker 2, so that the spotting marker 2 can be placed inside the culture dish 1, and the bottom wall of the spotting marker 2 is made of a transparent material, and a plurality of spotting marker positions 3 are arranged on the upper side of the bottom wall of the culture dish 1, and the plurality of spotting marker positions 3 are in a rectangular array;

[0024] The spot marking device also includes a plurality of spot marking units 4, which include a use state and a storage state. When the spot marking unit 4 is in the use state, it can be installed on any spot marking point 3. When the spot marking unit 4 is in the storage state, it is separated from the spot marking point 3 and can be stored separately.

[0025] At this time, according to the needs of the user, multiple spotting identification units 4 are assembled on the corresponding spotting identification points 3, and then the spotting identification device 2 is placed inside the culture dish 1, and the bottom wall of the spotting identification device 2 and the upper side of the spotting identification unit 4 are abutted against each other, so that the spotting identification unit 4 can be used as the identification area at the bottom wall of the spotting identification device 2, and the operator can drop the diluted sample of the yeast culture on the identification area at the bottom wall of the spotting identification device 2. The spotting process is more convenient, the sample points are evenly distributed, and by operating the spotting identification unit 4, the number of the spotting identification units 4 and the points to be spotted by the operator are the same, thereby reducing the phenomenon of multiple spots and wrong spots during the operator's spotting of the diluted sample of the yeast culture.

[0026] Among them, Figure 2-3As shown, the spot identification point 3 can be a magnetic block, and the spot identification unit 4 is magnetically attracted to the spot identification point 3. At the same time, the spot identification unit 4 can also be snapped onto the spot identification point 3. In the present technical solution, a snap-on installation method is preferred, which is that the spot identification point 3 includes a clamping ring 12 fixedly connected to the bottom wall of the culture dish 1, and the spot identification unit 4 includes a marking body, and the lower part of the marking body is fixedly connected with a clamping block 10, and the clamping block 10 can be snapped into the inside of the clamping ring 12. The spot identification unit 4 positioned in this way is not easy to move.

[0027] like Figure 2-4 As shown, in order to improve the stability of the fixed connection of the block 10 inside the clamping ring 12, a second magnet 13 is fixedly connected to the lower side of the clamping ring 12, and a first magnet 11 that is magnetically attracted to the second magnet 13 is fixedly connected to the lower side of the block 10. At this time, the block 10 can be fixed by the magnetic force between the first magnet 11 and the second magnet 13.

[0028] like Figure 1 and Figure 3-4 As shown, in order to reduce the processing accuracy of the block 10 and the clamping ring 12, an elastic gasket 14 is fixedly connected to the upper side of the clamping ring 12, and the outer diameter of the marker is larger than the outer diameter of the elastic gasket 14, so that the upper part of the elastic gasket 14 can be against the marker. At this time, the clamping ring 12 can move the marker upward. When the spot marker 2 is installed, multiple markers will be pressed down until the bottom wall of the spot marker 2 is simultaneously against multiple markers, so that multiple markers can be synchronously against the bottom wall of the spot marker 2.

[0029] like Figure 1-3 As shown, in order to enable the spot marker 2 to be stably placed on the upper side of the multiple spot marker units 4, two adjustment rods 5 are installed on the side wall of the culture dish 1, and a clamping piece 6 is installed at one end of the adjustment rod 5 located inside the culture dish 1. The two clamping pieces 6 can clamp and fix the side wall of the spot marker 2;

[0030] Here, the adjusting rod 5 can be a threaded rod threadedly connected to the side wall of the culture dish 1, and one end of the threaded rod located inside the culture dish 1 is rotatably connected to the clamping piece 6, that is, the clamping piece 6 can be driven to rotate by adjusting the threaded rod.

[0031] like Figure 3-4 As shown, the identification body includes an identification plate 7 fixedly connected to the upper side of a card block 10, and a sampling cross line 9 and a card block 10 located outside the sampling cross line 9 are provided on the upper side of the identification plate 7. The coordination of the sampling identification circle 8 and the sampling cross line 9 facilitates accurate sampling of the diluted sample.

[0032] The working principle of the utility model is as follows: a plurality of spotting identification units 4 are assembled on corresponding spotting identification points 3 according to the needs of the user, and then the spotting identification device 2 is placed inside the culture dish 1, and the bottom wall of the spotting identification device 2 and the upper side of the spotting identification unit 4 are abutted against each other, so that the spotting identification unit 4 can be used as the identification area at the bottom wall of the spotting identification device 2, and the operator can drop the diluted sample of the yeast culture on the identification area at the bottom wall of the spotting identification device 2;

[0033] In summary, the present technical solution operates the spotting identification units 4 so that the number of spotting identification units 4 and the spots to be spotted by the operator are the same, thereby reducing the phenomenon of multiple spots and wrong spots during the operator's spotting of the diluted yeast culture sample.

[0034] The above is only a preferred embodiment of the present invention. It should be noted that, for ordinary technicians in the field, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. A spot marking device for yeast cell culture, comprising a culture dish (1) and a spot marking device (2), wherein the culture dish (1) and the spot marking device (2) both comprise a side wall and a bottom wall, wherein the bottom wall is fixedly connected to the lower side of the side wall, and the inner diameter of the culture dish (1) is larger than the outer diameter of the spot marking device (2), and the bottom wall of the spot marking device (2) is made of a transparent material, characterized in that: A plurality of sample spot marking points (3) are arranged on the upper side of the bottom wall of the culture dish (1), and the plurality of sample spot marking points (3) are arranged in a rectangular array; The spot marking device further comprises a plurality of spot marking units (4), wherein the spot marking units (4) comprise a use state and a storage state. When the spot marking units (4) are in the use state, they can be installed on any spot marking point (3), and when the spot marking units (4) are in the storage state, they are separated from the spot marking point (3).

2. A spotting marking device for yeast cell culture according to claim 1, characterized in that: The sample spot identification point (3) comprises a clamping ring (12) fixedly connected to the bottom wall of the culture dish (1), and the sample spot identification unit (4) comprises an identification body, a clamping block (10) is fixedly connected to the lower part of the identification body, and the clamping block (10) can be clamped inside the clamping ring (12).

3. A spotting marking device for yeast cell culture according to claim 2, characterized in that: A second magnet (13) is fixedly connected to the lower side of the inner portion of the clamping ring (12), and a first magnet (11) that is magnetically attracted to the second magnet (13) is fixedly connected to the lower side of the clamping block (10).

4. A spotting marking device for yeast cell culture according to claim 2, characterized in that: An elastic gasket (14) is fixedly connected to the upper side of the clamping ring (12).

5. A spot marking device for yeast cell culture according to claim 2, characterized in that: The identification body comprises an identification plate (7) fixedly connected to the upper side of a card block (10), and a dotted cross line (9) and a card block (10) located outside the dotted cross line (9) are arranged on the upper side of the identification plate (7).

6. A spotting marking device for yeast cell culture according to claim 4, characterized in that: Two adjusting rods (5) are installed on the side wall of the culture dish (1), and a clamping piece (6) is installed on one end of the adjusting rod (5) located inside the culture dish (1).

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