Plantain herb in-vitro propagation device
By designing the plantain in vitro breeding device, the staggered plates and limit slot positioning, temperature and humidity detection and multi-band lamps are used to solve the problem of cumbersome space occupation and observation on large-scale breeding, and efficient management and environmental stability are achieved. Improve the accuracy of reproductive results and space utilization.
Patent Information
- Application Number
- CN202421014245.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-05-11
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-05-11
AI Technical Summary
During the large-scale ex vivo reproduction of plantain, the traditional single culture bottle method occupies a lot of space, which increases laboratory management and operation costs, and the observation of reproduction is complicated, which can easily lead to observation loopholes and affect the accuracy and reliability of reproduction results. sex.
A plantain ex vivo breeding device is designed, including multiple accommodating chambers formed by interlaced transverse plates and longitudinal plates, equipped with limit grooves and limit blocks for positioning, and a temperature and humidity detector and multi-band lamps are installed. Provides convenient observation panels and breathable holes to achieve simultaneous breeding of multiple samples and environmental monitoring.
The device improves the reproductive space efficiency through multiple accommodating chambers, ensures accurate sample positioning, stable monitoring environment, reduces space occupancy and management costs, and improves the accuracy and reliability of reproductive results.
Smart Images
Figure CN222193545U_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of in vitro propagation devices for plantain, and in particular to an in vitro propagation device for plantain. Background Art
[0002] Plantain is a common herbaceous plant. Its main propagation methods include seed propagation and asexual propagation. In vitro propagation is a plant biotechnology method that cultivates plant tissues or cells in a soilless or medium-free environment to achieve the propagation of new plants. In in vitro propagation, part of the plant tissue (such as stem tips, leaves, buds, roots, etc.) is removed and cultured in a culture medium containing appropriate nutrients and plant growth hormones. These tissues will grow and differentiate under sterile conditions, and eventually produce new plants. In vitro propagation of plantain refers to the use of tissues or cells of plantain plants in a soilless or medium-free culture environment, through the appropriate nutrients and plant growth hormones provided by the culture medium to achieve the propagation process of new plants.
[0003] In the current in vitro propagation process of plantain, the sterilized plantain tissue is first placed in a culture medium, which is usually placed in a culture bottle and sealed. Then, the culture bottles containing the culture medium and plantain tissue are placed on a shelf one by one for propagation. During the entire propagation process, the staff needs to observe the growth in the bottle.
[0004] However, when faced with large-scale in vitro propagation of plantain, a series of problems arise. First, a large number of bottles will take up a lot of space, increasing the management and operation costs of the laboratory. Second, a large number of bottles arranged on the shelves will make the staff's observation task cumbersome, because it takes a lot of time and energy to observe the growth of each bottle one by one. This observation process will consume a lot of human resources and easily lead to observation loopholes, thus affecting the accuracy and reliability of the breeding results.
[0005] Therefore, it is necessary to provide a plantain in vitro propagation device to solve the above problems.
[0006] It should be noted that the above information disclosed in this background technology section is only for understanding the background technology of the present application concept, and therefore, it may contain information that does not constitute the prior art. Summary of the invention
[0007] Based on the above problems existing in the prior art, the problem to be solved by the present application is: to provide a plantain in vitro propagation device, so as to achieve a more effective management effect on the large-scale plantain in vitro propagation process.
[0008] The technical solution adopted by the present application to solve the technical problem is: a plantago in vitro propagation device, including a propagation box, and also including:
[0009] A culture frame, which is arranged inside the breeding box;
[0010] A box cover, which is rotatably connected to one side of the breeding box;
[0011] A clamping piece, used to fix the box cover to the breeding box;
[0012] Among them, the interior of the culture frame is provided with transverse plates and longitudinal plates, and the transverse plates and longitudinal plates are provided in multiple groups. The multiple groups of transverse plates and longitudinal plates are arranged in a staggered manner to form multiple groups of accommodating cavities, which are used to place culture medium and plantain tissue. The front and rear sides of the breeding box are provided with a first observation panel, and the box cover is provided with a second observation panel.
[0013] Furthermore, the interior of the breeding box is symmetrically provided with limiting grooves, the outer side of the culture frame is provided with limiting blocks adapted to the limiting grooves, and the top surface of the limiting blocks is provided with a pull rod.
[0014] Furthermore, lamps are installed on the box cover, and two groups of lamps are installed.
[0015] Furthermore, a ventilation hole is provided at the upper end of the lamp, and a handle is provided at the upper end of the lamp.
[0016] Furthermore, the first observation panel and the lamp are both made of glass.
[0017] Furthermore, a temperature and humidity detector is installed inside the breeding box.
[0018] Furthermore, a groove is provided at the upper end of the breeding box, a slot connected to the groove is provided inside the breeding box, a slide groove connected to the slot is provided on one side of the breeding box, a push rod is slidably connected inside the slide groove, a pressing plate is provided at the end of the push rod, a second elastic member is provided on one side of the pressing plate, and one end of the second elastic member is connected to the breeding box.
[0019] Furthermore, the clamping part includes a fixing column arranged on the box cover, the fixing column is adapted to the groove, an inner cavity is opened on one side of the fixing column, a wedge block is slidably connected to the interior of the inner cavity, the wedge block is adapted to the clamping groove, and a first elastic part is arranged inside the inner cavity.
[0020] The beneficial effects of the present application are as follows: the present application provides a plantain in vitro propagation device, which provides sufficient propagation space for plantain through multiple accommodating cavities formed by multiple groups of staggered horizontal plates and longitudinal plates. Compared with the traditional propagation method of a single culture bottle, multiple samples can be cultured at the same time, thereby improving production efficiency and being more convenient for management. Secondly, the limiting grooves and limiting blocks can be used for accurate positioning, so that the culture frame can be accurately fixed in the propagation box, which makes the operation more convenient and avoids damage to the samples due to careless placement. Furthermore, the temperature and humidity detector can timely monitor the internal environmental conditions of the propagation box to ensure the stability of the culture environment. In addition, light sources of different bands are provided by lamps to promote the growth and development of plantain. Compared with the prior art, the present application allows multiple samples to be propagated in the propagation box at the same time, unlike the traditional method, where each sample requires a separate culture bottle, thereby saving a lot of space and reducing the management and operation costs of the laboratory.
[0021] In addition to the above-described purposes, features and advantages, the present application also has other purposes, features and advantages. The present application will be further described in detail with reference to the drawings. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] The drawings constituting part of the present application are used to provide a further understanding of the present application. The exemplary embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:
[0023] Figure 1 The overall schematic diagram of a plantain in vitro propagation device in this application Figure 1 ;
[0024] Figure 2 A schematic diagram of a culture frame of a plantain in vitro propagation device;
[0025] Figure 3 for Figure 1 A cross-sectional view of
[0026] Figure 4 for Figure 3 Enlarged view of point A in the middle;
[0027] Figure 5 for Figure 3 Enlarged view of point B in the middle;
[0028] Figure 6 The overall schematic diagram of a plantain in vitro propagation device Figure 2 ;
[0029] Among them, the reference numerals in the figure are:
[0030] 1. breeding box; 11. limiting groove; 12. temperature and humidity detector; 13. groove; 14. card slot; 15. slide slot; 151. top rod; 152. pressing plate; 16. second elastic member; 17. first observation panel; 2. culture frame; 21. horizontal plate; 22. longitudinal plate; 23. accommodating cavity; 24. limiting block; 241. pull rod; 3. box cover; 31. air vent; 32. lamp; 33. handle; 34. second observation panel; 4. card connector; 41. fixing column; 42. inner cavity; 43. wedge block; 44. first elastic member. DETAILED DESCRIPTION
[0031] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.
[0032] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present application.
[0033] like Figures 1 to 6 As shown, the present application provides a plantain in vitro propagation device, comprising a propagation box 1 and a culture frame 2 arranged inside the propagation box 1, a box cover 3 is rotatably connected to the upper end of the propagation box 1, a clamping member 4 is arranged on one side of the box cover 3, and the clamping member 4 is used to fix the box cover 3 to the propagation box 1;
[0034] like Figures 1 to 6As shown, a transverse plate 21 is arranged inside the culture frame 2, and multiple groups of transverse plates 21 are arranged. A longitudinal plate 22 is arranged inside the culture frame 2, and multiple groups of longitudinal plates 22 are arranged. The multiple groups of transverse plates 21 and the longitudinal plates 22 are arranged in a staggered manner to form multiple groups of accommodating chambers 23. The accommodating chambers 23 are used to place culture media and plantain tissues. The interior of the breeding box 1 is symmetrically provided with limiting grooves 11, and the outer side of the culture frame 2 is provided with limiting blocks 24. The limiting blocks 24 are adapted to the limiting grooves 11. The culture frame 2 is limited by the cooperation of the limiting blocks 24 and the limiting grooves 11. The top surface of the limiting block 24 is provided with a pull rod 241. The pull rod 241 facilitates the removal of the culture frame 2 from the inside of the breeding box 1. A temperature and humidity detector 12 is arranged inside the breeding box 1. The temperature and humidity detector 12 is used to detect the temperature and humidity inside the breeding box 1. The front and rear sides of the breeding box 1 are provided with a first observation panel 17. A second observation panel 34 is provided on the cover 3. Both the first observation panel 17 and the second observation panel 34 are made of glass material, so that the staff can observe the in vitro propagation process of plantain. An air vent 31 is provided on the second observation panel 34. The air vent 31 is used to maintain air circulation inside the breeding box 1 to ensure a certain amount of oxygen when the plantain is cultured in vitro. Lamps 32 are installed on the box cover 3. The lamps 32 are provided with two groups. The two groups of lamps 32 are white light LED lamps and red and blue light mixed LED lamps. The white light LED lamps are used to provide full-band white light to promote plant photosynthesis and overall growth. The red and blue light mixed LED lamps are used to provide a suitable spectrum combination to promote the overall production and development of the plant, thereby increasing the effect of in vitro propagation of plantain. A handle 33 is also provided on the second observation panel 34, which facilitates the staff to lift the breeding box 1 through the handle 33.
[0035] In the present embodiment, first, a suitable size of accommodating chamber 23 is selected according to actual needs, and the culture medium is placed in a plurality of accommodating chambers 23 respectively, and then the sterilized plantain tissues are placed in the accommodating chambers 23 one by one, and then the limiting block 24 is aligned with the limiting groove 11 and inserted to place the culture frame 2 in the breeding box 1, and then the box cover 3 is rotated to cover the breeding box 1, and the box cover 3 is fixed by the clamping piece 4, so as to complete the preliminary process of in vitro breeding of a plurality of plantains, and then the staff can lift the breeding box 1 by the handle 33 and place it in a suitable position, and the in vitro breeding process of plantain in a plurality of accommodating chambers 23 can be observed at one time by the first observation panel 17 and the second observation panel 34, which is more convenient than observing and taking a plurality of culture bottles for observation, and it is also convenient for the staff to observe The breeding process and changes inside each accommodating cavity 23 can be observed to prevent the occurrence of observation loopholes, further increasing the accuracy and reliability of the breeding results. At the same time, when plantain is propagated in vitro, the white light LED lamp on the box cover 3 provides full-band white light to promote the photosynthesis and overall growth of plantain. The red and blue light mixed LED lamp provides a suitable spectrum combination to promote the overall production and development of plantain, and the air vents 31 can provide sufficient oxygen during the plantain breeding process, thereby increasing the effect of in vitro breeding of plantain. The temperature and humidity detector 12 can monitor the temperature inside the breeding box 1 in real time, so that the staff can better know the temperature and humidity inside the breeding box 1 and make timely adjustments, further ensuring the growth environment of plantain during in vitro breeding, so as to improve the success rate and growth quality of in vitro breeding of plantain.
[0036] like Figure 3 and Figure 4 As shown, a groove 13 is provided at the upper end of the breeding box 1, a card slot 14 is provided on the inner wall of the groove 13, a slide groove 15 is provided on the inner wall of the card slot 14, one end of the slide groove 15 extends to the outside of the breeding box 1, a push rod 151 is slidably provided inside the slide groove 15, a pressing plate 152 is provided at the end of the push rod 151, a second elastic member 16 is provided on one side of the pressing plate 152, and the clamping member 4 includes a fixing column 41 provided on the box cover 3, the fixing column 41 is adapted to the groove 13, an inner cavity 42 is provided on one side of the fixing column 41, a wedge block 43 is slidably connected to the inner cavity 42, the wedge block 43 is adapted to the card slot 14, a first elastic member 44 is provided inside the inner cavity 42, and the first elastic member 44 and the second elastic member 16 are springs.
[0037] In this embodiment, when the box cover 3 is rotated to the top of the breeding box 1, the fixing column 41 is inserted into the inside of the groove 13, and the wedge block 43 is squeezed down on the inner wall of the groove 13, so that the wedge block 43 slides into the inner cavity 42, thereby squeezing the first elastic member 44. When the wedge block 43 is located on one side of the card slot 14, the wedge block 43 slides into the card slot 14 under the elastic force of the first elastic member 44, thereby fixing the fixing column 41 in the groove 13, preventing the box cover 3 from opening automatically when the staff lifts the handle 33. When the box cover 3 needs to be opened, the pressing plate 15 is pressed. 2. The pressing plate 152 drives the push rod 151 to move into the slot 14 and squeezes the second elastic member 16 at the same time. One end of the push rod 151 squeezes the wedge block 43 in the slot 14 into the inner cavity 42, so that the wedge block 43 is separated from the slot 14. At this time, the box cover 3 can be rotated to disengage the fixing column 41 from the groove 13, so that the box cover 3 can be opened. Then, the pressing plate 152 is released, and the push rod 151 is reset under the elastic force of the second elastic member 16, so that the wedge block 43 can be automatically engaged with the slot 14 when the box cover 3 is rotated to the top of the breeding box 1 next time.
[0038] In summary: the present application provides sufficient breeding space for plantain through multiple accommodating cavities 23 formed by multiple groups of staggered horizontal plates 21 and longitudinal plates 22. Compared with the traditional breeding method of a single culture bottle, multiple samples can be cultured at the same time to improve the production effect. Secondly, the limiting groove 11 and the limiting block 24 can be accurately positioned so that the culture frame 2 can be accurately fixed in the breeding box 1, which is more convenient to operate and avoids damage to the sample due to careless placement. Furthermore, the temperature and humidity detector 12 can monitor the internal environmental conditions of the breeding box 1 in time to ensure the stability of the culture environment. In addition, light sources of different bands are provided by the lamps 32 to promote the growth and development of plantain. Compared with the prior art, the present application allows multiple samples to be propagated in the breeding box 1 at the same time, unlike the traditional method, where each sample requires a separate culture bottle, thereby saving a lot of space and reducing the management and operation costs of the laboratory.
[0039] The above description is only the preferred embodiment of the present application and is not intended to limit the present application. For those skilled in the art, the present application may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.
Claims
1. A plantago in vitro propagation device, comprising a propagation box (1), characterized in that: Also includes: A culture frame (2), the culture frame (2) being arranged inside the breeding box (1); A box cover (3), the box cover (3) being rotatably connected to one side of the breeding box (1); A clamping member (4) for fixing the box cover (3) to the breeding box (1); The culture frame (2) is provided with a transverse plate (21) and a longitudinal plate (22) inside, and the transverse plate (21) and the longitudinal plate (22) are provided in a plurality of groups, the plurality of groups of transverse plates (21) and the longitudinal plates (22) are arranged in a staggered manner to form a plurality of groups of accommodating cavities (23), the accommodating cavities (23) being used to place culture medium and plantain tissue, the front and rear sides of the breeding box (1) are provided with a first observation panel (17), and the box cover (3) is provided with a second observation panel (34).
2. The in vitro propagation device for plantain according to claim 1, characterized in that: The breeding box (1) has symmetrically disposed limit grooves (11) inside, and the culture frame (2) has limit blocks (24) on the outside thereof adapted to the limit grooves (11), and a pull rod (241) is disposed on the top surface of the limit blocks (24).
3. The in vitro propagation device for plantain according to claim 2, characterized in that: The box cover (3) is equipped with lamps (32), and two groups of lamps (32) are installed.
4. The in vitro propagation device for plantain according to claim 3, characterized in that: An air vent (31) is provided at the upper end of the lamp (32), and a handle (33) is provided at the upper end of the lamp (32).
5. The in vitro propagation device for plantain according to claim 4, characterized in that: The first observation panel (17) and the lamp (32) are both components made of glass.
6. The in vitro propagation device for plantain according to claim 5, characterized in that: A temperature and humidity detector (12) is installed inside the breeding box (1).
7. The in vitro propagation device for plantain according to claim 1, characterized in that: The upper end of the breeding box (1) is provided with a groove (13), the interior of the breeding box (1) is provided with a slot (14) connected to the groove (13), one side of the breeding box (1) is provided with a slide groove (15) connected to the slot (14), the interior of the slide groove (15) is slidably connected with a push rod (151), the end of the push rod (151) is provided with a pressing plate (152), one side of the pressing plate (152) is provided with a second elastic member (16), and one end of the second elastic member (16) is connected to the breeding box (1).
8. The in vitro propagation device for plantain according to claim 7, characterized in that: The clamping member (4) comprises a fixing column (41) provided on the box cover (3), the fixing column (41) being matched with the groove (13), an inner cavity (42) being provided on one side of the fixing column (41), a wedge block (43) being slidably connected inside the inner cavity (42), the wedge block (43) being matched with the clamping groove (14), and a first elastic member (44) being provided inside the inner cavity (42).