Device for measuring activity of cells in cell culture

Abstract

The utility model relates to the technical field of cell viability determination, in particular to a cell viability determination device in a cell culture, which comprises a base, an LED (light-emitting diode) lamp panel is embedded in the middle of the top end of the base, a dyeing box is mounted at the top end of the base positioned at the top end of the LED lamp panel, support plates are arranged on two sides of the dyeing box, and the support plates are fixed on the base. A mounting plate is fixed to the top end of the supporting plate. The utility model has the beneficial effects that the light-avoiding ring is arranged at the top end of the rectangular frame so as to prevent external light from influencing the observation of cells in the rectangular frame, and the magnifying lens cylinder is used for observing and counting the cells selected in the rectangular frame; the rectangular frame can be driven to move forwards, backwards, leftwards and rightwards in the dyeing box, so that different positions can be selected for frame selection and counting of cells, stable arrangement of the rectangular frame and the magnifying lens barrel can be kept, and the situation that the accuracy of frame selection and counting is affected due to shaking and sliding of the rectangular frame caused by touching the magnifying lens barrel is avoided.

Description

Technical Field

[0001] The utility model relates to the technical field of cell viability determination, in particular to a device for determining cell viability in cell culture. Background Art

[0002] The conventional method for detecting cell viability is to add a fully diluted cell suspension to a Neubauer cell counting plate to determine the ratio of intact and damaged cells. The size of the cell counting plate roughly corresponds to a conventional microscope slide, and grooves of specific sizes are engraved on it to further divide the counting plate into different counting areas. Under a microscope, stained damaged cells and unstained intact cells can be counted. Usually, the number of cells in multiple counting areas is measured and averaged to reduce statistical errors. Then, the absolute amount of intact and damaged cells in the cell culture is converted by reverse calculation based on the known counting volume and the dilution ratio of the cell suspension.

[0003] Cell viability refers to the percentage of live cells in the total cells in a cell population, in which there are always some cells that have died for various reasons. Cell viability can be determined by the aniline black method, in which dead cells are dyed black by a dye, while live cells are not colored.

[0004] For example, the prior art Chinese patent publication number "CN220188324U" provides a cell viability assay device in cell culture, including a staining box, which is a transparent box body, a feeding tube is connected to one side of the staining box, and two bottom plates are fixedly connected to the bottom of the staining box.

[0005] The device can be rotated and adjusted in height through the cross bar, so that the mounting block movably installed at one end of the cross bar can be placed vertically, so that the magnifying glass barrel is facing the stained cells at the bottom, and one end of the clamping plate is pressed to release the lock of the adjusting rod at the other end of the clamping plate, so that the rectangular frame at the bottom of the adjusting rod can frame the target area, and the magnified observation is carried out through the magnifying glass barrel, and then the respective number ratios of the stained black cells and the unstained black cells are observed, and then the cell viability measurement value is directly observed. The transparent staining box cooperates with the lamp moved by the sliding plate to illuminate the bottom for easy observation.

[0006] In the existing cell culture cell viability measurement device, it is not convenient to adjust the height position of the rectangular frame during the process of framing cells inside the staining box, and it is not convenient to accurately frame the cells inside the staining box. Moreover, it is also inconvenient to adjust the height position of the magnifying glass. At the same time, the rectangular frame is relatively fixed during the process of framing cells, and it is not convenient to move it forward, backward, left, and right to adjust different positions, and it is impossible to frame different positions inside the staining box. Utility Model Content

[0007] The purpose of the utility model is to solve the shortcomings of the prior art that it is inconvenient to adjust the height position of the rectangular frame during the process of framing the cells inside the staining box, it is inconvenient to accurately frame the cells inside the staining box, and it is also inconvenient to adjust the height position of the magnifying glass. A cell viability determination device in cell culture is proposed.

[0008] In order to achieve the above purpose, the utility model provides the following technical solutions:

[0009] A cell viability assay device in cell culture is designed, comprising a base, an LED light board is embedded in the middle of the top of the base, and a staining box is installed on the top of the base located at the top of the LED light board, support plates are arranged on both sides of the staining box, a mounting plate is fixed to the top of the support plate, a mounting groove is opened in the middle of the mounting plate, a mounting block is slidably installed in the middle of the mounting groove, a plug-in groove is opened in the middle of the mounting block, a magnifying lens barrel passes through the inside of the plug-in groove, an adjusting tube is connected to the outer surface of the magnifying lens barrel, and the adjusting tube passes through the plug-in groove by threaded cooperation, guide holes are opened at the four corners of the surface of the mounting block, a guide rod passes through the inside of the guide hole, nuts are connected to the surface of the guide rod and at the upper and lower ends of the guide hole, a rectangular frame is fixed to the bottom end of the guide rod, and a light-shielding ring is surrounded by the outer side of the guide rod and at the top of the rectangular frame.

[0010] Furthermore, adjustment grooves are opened at both ends of the installation groove, and adjustment rods are fixed at both ends of the installation block. The adjustment rods slide through the adjustment grooves. A first connecting hole is opened at the top of the adjustment groove. A first bolt passes through the inside of the first connecting hole, and the bottom end of the first bolt supports the top of the adjustment rod.

[0011] Furthermore, a slide groove is opened at the top of the base and at the positions on both sides of the dyeing box, and the bottom end of the support plate slides through the slide groove. A second connecting hole is opened on the outside of the slide groove, and a second bolt passes through the inside of the second connecting hole. The inner end of the second bolt presses the support plate tightly.

[0012] Furthermore, the dyeing box adopts a transparent box structure, the support plate is a rectangular structure and is vertically arranged, the mounting plate is a rectangular structure and is horizontally arranged, and the mounting groove is a rectangular groove structure.

[0013] Furthermore, the plug-in slot is an internal threaded slot structure, the adjusting tube is an external threaded tube structure, the magnifying lens tube is threadedly connected to the plug-in slot through the adjusting tube, and is vertically arranged.

[0014] Furthermore, the guide rod is a threaded rod structure and is vertically arranged, the rectangular frame is a rectangular frame structure and is horizontally arranged inside the dyeing box, and the light-shielding circle is a rectangular circle structure.

[0015] Furthermore, the cross-sections of the adjustment slot and the adjustment rod are both rectangular structures, and the adjustment rod is a long rod structure, which is horizontally arranged, and the first connecting hole is a threaded hole structure.

[0016] Furthermore, the slide groove is a long strip groove structure, and the second connecting hole is a threaded hole structure.

[0017] The utility model provides a device for measuring cell viability in cell culture, which has the following beneficial effects:

[0018] 1. The utility model sets a mounting block by sliding an adjusting rod in a mounting groove in the middle of a mounting plate, a magnifying lens barrel passes through the plug-in groove through the adjusting tube threaded fit, and a rectangular frame is set below the mounting block through a guide rod. Thus, during the process of measuring the viability of cells, the nut can be loosened, and the height position of the guide rod and the rectangular frame at the bottom can be adjusted along the guide hole, so that the rectangular frame is close to the inner bottom end of the staining box and close to the evenly spread cells. At the same time, the adjusting tube is rotated to adjust the height position of the magnifying lens barrel, thereby adjusting the height of the magnifying lens from the cells. Therefore, a light-shielding ring is set at the top of the rectangular frame to block the influence of external light on the observed cells inside the rectangular frame, and the cells selected inside the rectangular frame can be observed and counted through the magnifying lens barrel.

[0019] 2. The support plate of the utility model can be slid forward and backward and installed on the base through a slide groove, and the mounting block can be slid left and right and installed on the mounting plate through an adjusting rod. During the process of measuring the viability of cells, the position of the support plate can be moved forward and backward along the slide groove, and the position of the mounting block can be moved left and right along the mounting groove, and they are respectively fastened and stably set by the second bolt and the first bolt. In this way, the rectangular frame can be driven to move forward and backward, left and right inside the staining box to select different positions for framing cells and counting, and the rectangular frame and the magnifying lens barrel can be kept stably set to avoid the rectangular frame sliding and shaking due to touching the magnifying lens barrel, thereby affecting the accuracy of the frame selection and counting. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the overall structure of the utility model;

[0021] Figure 2 For this utility model Figure 1 The enlarged view of point D;

[0022] Figure 3 For this utility model Figure 1 The enlarged view of point E;

[0023] Figure 4For this utility model Figure 1 The enlarged view of F;

[0024] Figure 5 For this utility model Figure 1 A cross-sectional view of

[0025] Figure 6 For this utility model Figure 5 A magnified image of point A;

[0026] Figure 7 For this utility model Figure 5 A magnified view of point B;

[0027] Figure 8 For this utility model Figure 5 Enlarged view of point C.

[0028] In the figure: 1. base; 2. LED light board; 3. rectangular frame; 4. dyeing box; 5. support plate; 6. second bolt; 7. light-shielding circle; 8. mounting groove; 9. mounting plate; 10. adjustment rod; 11. magnifying lens tube; 12. adjustment tube; 13. guide rod; 14. mounting block; 15. plug-in slot; 16. nut; 17. guide hole; 18. first connecting hole; 19. first bolt; 20. adjustment slot; 21. second connecting hole; 22. slide slot. DETAILED DESCRIPTION

[0029] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0030] Example 1

[0031] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8, a cell culture cell viability assay device shown in the figure comprises a base 1, an LED light board 2 is inlaid in the middle of the top of the base 1, and a staining box 4 is installed on the top of the base 1 located at the top of the LED light board 2, support plates 5 are arranged on both sides of the staining box 4, a mounting plate 9 is fixed on the top of the support plate 5, a mounting groove 8 is opened in the middle of the mounting plate 9, a mounting block 14 is slidably installed in the middle of the mounting groove 8, a plug-in groove 15 is opened in the middle of the mounting block 14, and the inner portion of the plug-in groove 15 is A magnifying lens barrel 11 passes through the part, and an adjusting barrel 12 is connected to the outer surface of the magnifying lens barrel 11. The adjusting barrel 12 is threadedly fitted through the plug-in slot 15. Guide holes 17 are opened at the four corners of the surface of the mounting block 14. A guide rod 13 passes through the inside of the guide hole 17. Nuts 16 are connected to the surface of the guide rod 13 and at the upper and lower ends of the guide hole 17. A rectangular frame 3 is fixed to the bottom end of the guide rod 13, and a light-shielding circle 7 is arranged around the outer side of the guide rod 13 and at the top of the rectangular frame 3.

[0032] During the process of measuring the viability of cells, the nut 16 can be loosened and the height position of the guide rod 13 and the bottom rectangular frame 3 can be adjusted along the guide hole 17 so that the rectangular frame 3 is close to the inner bottom end of the staining box 4 and close to the evenly spread cells. At the same time, the adjusting tube 12 is rotated to adjust the height position of the magnifying lens tube 11, thereby adjusting the height of the magnifying lens from the cells. Therefore, the light-shielding circle 7 is set at the top of the rectangular frame 3 to block the influence of external light on the observed cells inside the rectangular frame 3, and the cells selected inside the rectangular frame 3 are observed and counted through the magnifying lens tube 11.

[0033] Adjustment grooves 20 are formed at both ends of the mounting groove 8, and adjustment rods 10 are fixed at both ends of the mounting block 14. The adjustment rod 10 slides through the adjustment groove 20. A first connecting hole 18 is formed at the top of the adjustment groove 20. A first bolt 19 passes through the inside of the first connecting hole 18, and the bottom end of the first bolt 19 supports the top end of the adjustment rod 10.

[0034] During the process of measuring the viability of cells, the position of the support plate can be moved forward and backward along the slide groove 22, the position of the mounting block 14 can be moved left and right along the mounting groove 8, and they can be fastened and stabilized by the second bolt 6 and the first bolt 19 respectively. In this way, the rectangular frame 3 can be driven to move forward and backward, left and right inside the staining box 4 to facilitate the selection of different positions for framing and counting cells, and the rectangular frame 3 and the magnifying lens barrel 11 can be kept stable to avoid the rectangular frame 3 sliding and shaking due to touching the magnifying lens barrel 11, thereby affecting the accuracy of cell selection and counting.

[0035] The dyeing box 4 adopts a transparent box structure, the support plate 5 is a rectangular structure and is vertically arranged, the mounting plate 9 is a rectangular structure and is horizontally arranged, and the mounting groove 8 is a rectangular groove structure; the plug-in groove 15 is an internal thread groove structure, the adjusting cylinder 12 is an external thread cylinder structure, and the magnifying lens barrel 11 is threadedly connected to the plug-in groove 15 through the adjusting cylinder 12 and is vertically arranged.

[0036] The guide rod 13 is a threaded rod structure and is vertically arranged. The rectangular frame 3 is a rectangular frame structure and is horizontally arranged inside the dyeing box 4. The light-shielding circle 7 is a rectangular circle structure. The cross-sections of the adjustment groove 20 and the adjustment rod 10 are both rectangular structures, and the adjustment rod 10 is a long rod structure, which is horizontally arranged. The first connecting hole 18 is a threaded hole structure.

[0037] Example 2

[0038] See also Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 , a cell culture cell viability assay device shown in the figure comprises a base 1, an LED light board 2 is inlaid in the middle of the top of the base 1, and a staining box 4 is installed on the top of the base 1 located at the top of the LED light board 2, support plates 5 are arranged on both sides of the staining box 4, a mounting plate 9 is fixed on the top of the support plate 5, a mounting groove 8 is opened in the middle of the mounting plate 9, a mounting block 14 is slidably installed in the middle of the mounting groove 8, a plug-in groove 15 is opened in the middle of the mounting block 14, and the inner portion of the plug-in groove 15 is A magnifying lens barrel 11 passes through the part, and an adjusting barrel 12 is connected to the outer surface of the magnifying lens barrel 11. The adjusting barrel 12 is threadedly fitted through the plug-in slot 15. Guide holes 17 are opened at the four corners of the surface of the mounting block 14. A guide rod 13 passes through the inside of the guide hole 17. Nuts 16 are connected to the surface of the guide rod 13 and at the upper and lower ends of the guide hole 17. A rectangular frame 3 is fixed to the bottom end of the guide rod 13, and a light-shielding circle 7 is arranged around the outer side of the guide rod 13 and at the top of the rectangular frame 3.

[0039] During the process of measuring the viability of cells, the nut 16 can be loosened and the height position of the guide rod 13 and the bottom rectangular frame 3 can be adjusted along the guide hole 17 so that the rectangular frame 3 is close to the inner bottom end of the staining box 4 and close to the evenly spread cells. At the same time, the adjusting tube 12 is rotated to adjust the height position of the magnifying lens tube 11, thereby adjusting the height of the magnifying lens from the cells. Therefore, the light-shielding circle 7 is set at the top of the rectangular frame 3 to block the influence of external light on the observed cells inside the rectangular frame 3, and the cells selected inside the rectangular frame 3 are observed and counted through the magnifying lens tube 11.

[0040] Adjustment grooves 20 are formed at both ends of the mounting groove 8, and adjustment rods 10 are fixed at both ends of the mounting block 14. The adjustment rod 10 slides through the adjustment groove 20. A first connecting hole 18 is formed at the top of the adjustment groove 20. A first bolt 19 passes through the inside of the first connecting hole 18, and the bottom end of the first bolt 19 supports the top end of the adjustment rod 10.

[0041] A slide groove 22 is provided at the top of the base 1 and at positions on both sides of the dyeing box 4. The bottom end of the support plate 5 slides through the slide groove 22. A second connecting hole 21 is provided on the outer side of the slide groove 22. A second bolt 6 passes through the inside of the second connecting hole 21. The inner end of the second bolt 6 presses against the support plate 5.

[0042] During the process of measuring the viability of cells, the position of the support plate can be moved forward and backward along the slide groove 22, the position of the mounting block 14 can be moved left and right along the mounting groove 8, and they can be fastened and stabilized by the second bolt 6 and the first bolt 19 respectively. In this way, the rectangular frame 3 can be driven to move forward and backward, left and right inside the staining box 4 to facilitate the selection of different positions for framing and counting cells, and the rectangular frame 3 and the magnifying lens barrel 11 can be kept stable to avoid the rectangular frame 3 sliding and shaking due to touching the magnifying lens barrel 11, thereby affecting the accuracy of cell selection and counting.

[0043] The sliding groove 22 is a long strip groove structure, and the second connecting hole 21 is a threaded hole structure.

[0044] Working method: The utility model slides the mounting block 14 in the mounting groove 8 in the middle of the mounting plate 9 through the adjusting rod 10, and the magnifying lens barrel 11 passes through the plug-in groove 15 through the threaded cooperation of the adjusting tube 12. The rectangular frame 3 is set below the mounting block 14 through the guide rod 13. In this way, during the process of measuring the vitality of the cells, the nut 16 can be loosened, and the height position of the guide rod 13 and the rectangular frame 3 at the bottom can be adjusted along the guide hole 17, so that the rectangular frame 3 is close to the inner bottom end of the staining box 4 and close to the evenly spread cells. At the same time, the adjusting tube 12 is rotated to adjust the height position of the magnifying lens barrel 11, thereby adjusting the height of the magnifying glass from the cells. Therefore, the light-shielding circle 7 is set at the top of the rectangular frame 3 to block the influence of external light on the observed cells inside the rectangular frame 3, and the cells selected inside the rectangular frame 3 are observed and counted through the magnifying lens barrel 11.

[0045] The support plate 5 can be slid forward and backward and installed on the base 1 through the slide groove 22, and the mounting block 14 can be slid left and right and installed on the mounting plate 9 through the adjustment rod 10. During the cell viability measurement process, the position of the support plate 5 can be moved forward and backward along the slide groove 22, and the position of the mounting block 14 can be moved left and right along the mounting groove 8, and they are respectively fastened and stably set by the second bolt 6 and the first bolt 19. In this way, the rectangular frame 3 can be driven to move forward and backward and left and right inside the staining box 4 to facilitate the selection of different positions for framing cells and counting, and the rectangular frame 3 and the magnifying lens barrel 11 can be kept stably set to avoid the rectangular frame 3 sliding and shaking due to touching the magnifying lens barrel 11, thereby affecting the accuracy of cell framing and counting.

[0046] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A device for measuring cell viability in cell culture, comprising a base (1), characterized in that: An LED light board (2) is embedded in the middle of the top of the base (1), and a dyeing box (4) is installed at the top of the base (1) located at the top of the LED light board (2). Support plates (5) are arranged on both sides of the dyeing box (4). A mounting plate (9) is fixed to the top of the support plate (5). A mounting groove (8) is opened in the middle of the mounting plate (9). A mounting block (14) is slidably installed in the middle of the mounting groove (8). A plug-in groove (15) is opened in the middle of the mounting block (14). A magnifying lens tube (11) passes through the inside of the plug-in groove (15). The outer surface of the magnifying lens tube (11) is connected to an adjusting tube (12), the adjusting tube (12) is threadedly fitted through the inserting slot (15), the surface of the mounting block (14) is provided with guide holes (17) at four corners, a guide rod (13) passes through the inside of the guide hole (17), nuts (16) are connected to the surface of the guide rod (13) and at the upper and lower ends of the guide hole (17), a rectangular frame (3) is fixed to the bottom end of the guide rod (13), and a light-shielding ring (7) surrounds the outer side of the guide rod (13) and the top end of the rectangular frame (3).

2. The device for measuring cell viability in cell culture according to claim 1, characterized in that: Adjustment grooves (20) are formed at both ends of the installation groove (8), and adjustment rods (10) are fixed at both ends of the installation block (14). The adjustment rod (10) slides through the adjustment groove (20), and a first connecting hole (18) is formed at the top of the adjustment groove (20). A first bolt (19) passes through the inside of the first connecting hole (18), and the bottom end of the first bolt (19) abuts against the top end of the adjustment rod (10).

3. The device for measuring cell viability in cell culture according to claim 1, characterized in that: A slide groove (22) is provided at the top of the base (1) and at positions on both sides of the dyeing box (4), and the bottom end of the support plate (5) slides through the slide groove (22). A second connecting hole (21) is provided on the outside of the slide groove (22), and a second bolt (6) passes through the inside of the second connecting hole (21), and the inner end of the second bolt (6) presses against the support plate (5).

4. The device for measuring cell viability in cell culture according to claim 1, characterized in that: The dyeing box (4) adopts a transparent box structure, the support plate (5) is a rectangular structure and is arranged vertically, the mounting plate (9) is a rectangular structure and is arranged horizontally, and the mounting groove (8) is a rectangular groove structure.

5. The device for measuring cell viability in cell culture according to claim 1, characterized in that: The inserting groove (15) is an internal thread groove structure, the adjusting tube (12) is an external thread tube structure, and the magnifying lens tube (11) is threadably connected to the inserting groove (15) through the adjusting tube (12) and is vertically arranged.

6. The device for measuring cell viability in cell culture according to claim 1, characterized in that: The guide rod (13) is a threaded rod structure and is vertically arranged; the rectangular frame (3) is a rectangular frame structure and is horizontally arranged inside the dyeing box (4); and the light-shielding circle (7) is a rectangular circle structure.

7. The device for measuring cell viability in cell culture according to claim 2, characterized in that: The cross-sections of the adjustment groove (20) and the adjustment rod (10) are both rectangular structures, and the adjustment rod (10) is a long bar structure which is arranged horizontally, and the first connection hole (18) is a threaded hole structure.

8. The device for measuring cell viability in cell culture according to claim 3, characterized in that: The sliding groove (22) is a long strip groove structure, and the second connecting hole (21) is a threaded hole structure.

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