Organ chip for multi-tissue co-culture
By designing a multi-tissue co-culture organ chip, using the combination of modular structure and limiting pins, flexible contact between multiple cells and drugs is achieved, solving the problems of low efficiency and inaccurate results of single cell-like culture in the prior art , improves the efficiency of drug screening and the reliability of results.
Patent Information
- Application Number
- CN202420897499.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-04-26
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-04-26
AI Technical Summary
The existing organ chips are mainly two-dimensional cultures of a single cell-like cell type, which makes it difficult to simulate the microenvironment of natural organs, resulting in the inconsistent drug screening results with in vivo tests. The closed culture limits the analysis and detection of cells and proteins, which is inefficient and difficult to achieve. Multiple screening of medicine.
Design a multi-tissue co-culture organ chip, including substrate, first module, second module, transparent cover and limit latch, allowing flexibility of different cells and drugs through the design of multiple storage holes, channels and storage tanks Splicing and contacting to simulate the drug resistance experiments of multiple cells to different drugs.
The experimental staff's workload on the arrangement and combination of multiple cells and multiple drugs has been significantly reduced, the experimental efficiency has been improved, repeated proportions have been avoided, and the efficiency of the drug screening process and the reliability of the results have been enhanced.
Smart Images
Figure CN222205272U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the technical field of microfluidic organ chips, in particular to a multi-tissue co-culture organ chip. Background Art
[0002] Organ chip is the abbreviation of microfluidic organ chip. It is a multi-channel three-dimensional cell culture device with a continuous perfusion chamber. It consists of two parts. One part is the main body built by chips and cells according to the proportion and order of natural organs, and the other part includes the microenvironment composed of cells, secretions and physical forces around the corresponding organs. With the development of modern medicine and modern pharmacology, more and more drug screening models prepared by animal tissues and organs are used, such as in vitro vascular experiments, cardiac perfusion experiments, tissue culture experiments and other methods. However, this model is small in scale, low in efficiency, limited in drug effect, and requires a large amount of samples. It is not easy to achieve multiple screenings for one drug. In addition, the high requirements for manual operation technology are also one of the main reasons affecting its application in drug screening.
[0003] At present, after nearly ten years of development, a variety of human organ chips have been developed, such as liver chips, lung chips, intestine chips, kidney chips, blood vessel chips, heart chips, etc. However, most chips are two-dimensional cultures of a single type of cells, which are still far from the in vivo microenvironment of natural organs. It is difficult to obtain similar or identical results to in vivo tests when used for drug screening, and most of the cells in the chip are cultured in a closed manner, which limits the analysis and detection of cells and proteins during drug screening. Therefore, a multi-tissue co-cultured organ chip is proposed to optimize and solve the above problems. Utility Model Content
[0004] The purpose of the utility model is to provide an organ chip for multi-tissue co-culture to solve the problems raised in the above background technology.
[0005] In order to achieve the above purpose, the utility model provides the following technical solutions:
[0006] A multi-tissue co-culture organ chip comprises a chip body, wherein the chip body comprises a substrate, a first module, a second module, a transparent protective cover and a limiting latch, wherein the transparent protective cover is jointly covered on the outside of the first module and the second module, the limiting latches are symmetrically connected to the bottom of the first module and the second module respectively, a plurality of the limiting latches vertically penetrate the substrate, a plurality of first storage holes are equidistantly provided at the center of the first module, two first receiving grooves are symmetrically provided on the first module, a plurality of first channels are respectively provided on the first module, a plurality of first partitions are fixedly connected at an equal distance inside the first receiving groove, the first receiving groove is divided into a plurality of first channels by the plurality of the first partitions, a plurality of second storage holes are equidistantly provided on the second module, a second receiving groove is provided on the second module, a plurality of second channels are respectively provided on the second module, a plurality of second partitions are fixedly connected at an equal distance inside the second receiving groove, the second receiving groove is divided into a plurality of second channels by the plurality of the second partitions.
[0007] As a further solution of the utility model: the bottom of the first module is in contact with the top of the substrate, and multiple second modules are symmetrically arranged, and the bottoms of two of the multiple second modules are in contact with the top of the substrate, and the opposite sides of the two second modules are tightly in contact with the outer sides of the first module.
[0008] As a further solution of the utility model: the bottom of the transparent protective cover is fitted with the top of the base plate, and a limiting slot for matching with the limiting latch is provided on the base plate.
[0009] As a further solution of the utility model: both ends of the plurality of first channels are respectively connected to the plurality of first storage holes and the first accommodating grooves, and both ends of the plurality of second channels are respectively connected to the plurality of second storage holes and the second accommodating grooves.
[0010] As a further solution of the present invention: a plurality of the second grooves are adaptively connected with a plurality of the first grooves.
[0011] Compared with the prior art, the beneficial effects of the utility model are:
[0012] Different drugs and different cells are placed in the first storage hole in the first module and the second storage holes in the second modules respectively, and then the first module and two of the second modules are arbitrarily spliced on the substrate using limit pins, so that the multiple cells on the second module can be contacted through the first channel, the first receiving groove, the second channel and the second receiving groove. This makes it convenient to study the drug resistance experiments of multiple different cells against the same or different drugs, which can significantly reduce the workload of experimental staff in arranging and combining multiple cells and multiple drugs, avoid repeated matching between multiple cells and multiple drugs, and further improve the efficiency of experimental work. BRIEF DESCRIPTION OF THE DRAWINGS
[0013] Figure 1 Schematic diagram of the structure of the organ chip for multi-tissue co-culture.
[0014] Figure 2 Schematic diagram of the top view structure of the organ chip for multi-tissue co-culture.
[0015] Figure 3 Schematic diagram of the local structure in the organ chip for multi-tissue co-culture.
[0016] Figure 4 Schematic diagram of the local structure in the organ chip for multi-tissue co-culture.
[0017] As shown in the figure: chip body 1, substrate 2, first module 3, second module 4, transparent cover 5, limit pin 6, first storage hole 7, first channel 8, first accommodating groove 9, first partition 10, first groove 11, second storage hole 12, second channel 13, second accommodating groove 14, second partition 15 and second groove 16. DETAILED DESCRIPTION
[0018] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.
[0019] See also Figures 1 to 4In an embodiment of the utility model, an organ chip for multi-tissue co-culture includes a chip body 1, a substrate 2, a first module 3, a second module 4, a transparent protective cover 5, a limiting latch 6, a first storage hole 7, a first channel 8, a first receiving groove 9, a first partition 10, a first groove 11, a second storage hole 12, a second channel 13, a second receiving groove 14, a second partition 15 and a second groove 16. The chip body 1 includes a substrate 2, a first module 3, a second module 4, a transparent protective cover 5 and a limiting latch 6. The bottom of the first module 3 is in contact with the top of the substrate 2. The second modules 4 are symmetrically arranged in plurality, and the bottoms of two of the plurality of second modules 4 are both in contact with the top of the substrate 2, and the opposite sides of the two second modules 4 are tightly in contact with the outer side of the first module 3, and the transparent protective cover 5 is jointly covered on the outer sides of the first module 3 and the second module 4, and the bottom of the transparent protective cover 5 is in contact with the top of the substrate 2, and the limit pins 6 are symmetrically connected to the bottoms of the first module 3 and the second module 4, respectively, and the plurality of limit pins 6 vertically penetrate the substrate 2, and the substrate 2 is provided with limit slots for matching with the limit pins 6.
[0020] A plurality of first storage holes 7 are equidistantly provided at the center position of the first module 3, two first receiving grooves 9 are symmetrically provided on the first module 3, a plurality of first channels 8 are respectively provided on the first module 3, and the two ends of the plurality of first channels 8 are respectively communicated with the plurality of first storage holes 7 and the first receiving grooves 9, a plurality of first partitions 10 are fixedly connected at equal distances inside the first receiving groove 9, and the first receiving groove 9 is divided into a plurality of first grooves 11 by the plurality of first partitions 10, a plurality of second storage holes 12 are equidistantly provided on the second module 4, a second receiving groove 14 is provided on the second module 4, a plurality of second channels 13 are respectively provided on the second module 4, and the two ends of the plurality of second channels 13 are respectively communicated with the plurality of second storage holes 12 and the second receiving groove 14, a plurality of second partitions 15 are fixedly connected at equal distances inside the second receiving groove 14, and the second receiving groove 14 is divided into a plurality of second grooves 16 by the plurality of second partitions 15, and the plurality of second grooves 16 are adaptively matched with the plurality of first grooves 11.
[0021] The working principle of the utility model is:
[0022] When it is necessary to use an organ chip for multi-tissue co-culture, the experimental staff can first put different drugs into the first storage hole 7 in the first module 3 according to different actual use needs, and further put different cells into the second storage holes 12 in multiple second modules 4 respectively, and then arbitrarily splice the first module 3 and two of the second modules 4 on the substrate 2 using the limit pin 6, and then the various cells on the second module 4 are contacted through the first channel 8, the first receiving groove 9, the second channel 13 and the second receiving groove 14. Then, by covering the transparent protective cover 6 and continuously observing, it is convenient to study the drug resistance experiments of various different cells against the same or different drugs. It can also be paired by replacing different first modules 3 and second modules 4, so as to facilitate personnel to arrange and combine various cells and drugs, which can significantly reduce the workload of experimental staff in arranging and combining various cells and drugs, and avoid repeated matching between various cells and drugs, thereby further improving the experimental work efficiency.
[0023] Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art may still modify the technical solutions described in the aforementioned embodiments, or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.
Claims
1. An organ chip for multi-tissue co-culture, comprising a chip body (1), characterized in that: The chip body (1) comprises a substrate (2), a first module (3), a second module (4), a transparent protective cover (5) and a limit pin (6); the transparent protective cover (5) is jointly arranged on the outside of the first module (3) and the second module (4); the limit pins (6) are symmetrically connected to the bottom of the first module (3) and the second module (4); a plurality of limit pins (6) vertically penetrate the substrate (2); a plurality of first storage holes (7) are equidistantly provided at the center of the first module (3); two first accommodating grooves (9) are symmetrically provided on the first module (3); a plurality of first channels (8) are respectively provided on the first module (3); A plurality of first partitions (10) are fixedly connected at equal distances inside the first receiving groove (9), and the interior of the first receiving groove (9) is divided into a plurality of first grooves (11) by the plurality of first partitions (10), a plurality of second storage holes (12) are provided at equal distances on the second module (4), a second receiving groove (14) is provided on the second module (4), and a plurality of second channels (13) are respectively provided on the second module (4), a plurality of second partitions (15) are fixedly connected at equal distances inside the second receiving groove (14), and the interior of the second receiving groove (14) is divided into a plurality of second grooves (16) by the plurality of second partitions (15).
2. The multi-tissue co-culture organ chip according to claim 1, characterized in that: The bottom of the first module (3) is in contact with the top of the substrate (2), and a plurality of the second modules (4) are symmetrically arranged, wherein the bottoms of two of the plurality of second modules (4) are in contact with the top of the substrate (2), and the opposite sides of the two second modules (4) are in close contact with the outer sides of the first module (3).
3. The multi-tissue co-culture organ chip according to claim 1, characterized in that: The bottom of the transparent protective cover (5) is in contact with the top of the base plate (2), and a limiting slot for matching with the limiting latch (6) is provided on the base plate (2).
4. The multi-tissue co-culture organ chip according to claim 1, characterized in that: The two ends of the plurality of first channels (8) are respectively communicated with the plurality of first storage holes (7) and the first receiving groove (9), and the two ends of the plurality of second channels (13) are respectively communicated with the plurality of second storage holes (12) and the second receiving groove (14).
5. The multi-tissue co-culture organ chip according to claim 1, characterized in that: The plurality of the second grooves (16) are matched and connected with the plurality of the first grooves (11).