Muscle strip fixing device
By setting electrodes in the muscle strip fixation device to form an electric field, the problem that existing devices cannot adapt to receptor agonists that inhibit muscle strip contraction is solved, effectively measuring drugs that inhibit muscle strip contraction is achieved, and the receptor agonists are broadened Test range.
Patent Information
- Application Number
- CN202421297648.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2024-06-07
- Publication Date
- 2024-12-20
- Estimated Expiration
- 2034-06-07
AI Technical Summary
Existing muscle bar fixation devices are unable to adapt to receptor agonists that inhibit muscle bar contraction, resulting in a limited range of testing, especially for drugs such as morphine that enhances muscle tone but inhibits peristalsis.
A muscle strip fixation device is designed to form an electric field to amplify the contraction amplitude of the muscle strip, thereby adapting to receptor agonists that inhibit muscle strip contraction and broadening the testable receptors. Range of agonist drugs.
By increasing electric field stimulation, the contraction amplitude of the muscle bar is significantly amplified, ensuring effective measurement of drugs that inhibit muscle bar contraction is broadened, the range of tests for receptor agonists is broadened, and the sensitivity and accuracy of the experiment is improved.
Smart Images
Figure CN222212739U_ABST
Abstract
Description
Technical Field
[0001] The utility model relates to the field of medical instruments, in particular to a muscle strip fixing device. Background Art
[0002] The experiment of "Determination of affinity index (pD2) of receptor agonists and antagonist parameter (pA2) of antagonists" is a classic quantitative determination experiment in the pharmacology module of medical functional experimental teaching. In the experiment, one end of the isolated muscle strip needs to be fixed to the bottom of the bath, and the other end needs to be connected to the tension transducer.
[0003] The muscle strip fixture currently used in the experiment can test a limited range of receptor agonist drugs. This receptor agonist must be used to promote muscle strip contraction after activating the receptor. If the receptor agonist produces an inhibitory effect after activating the receptor, the muscle strip fixture is no longer applicable. For example, morphine is a μ receptor agonist, but after activating the μ receptor on the gastrointestinal smooth muscle, it will increase muscle tension and inhibit the peristalsis of the gastrointestinal smooth muscle. In the initial state (without adding receptor agonists), the contraction amplitude of smooth muscle is originally very small. After adding morphine, the contraction amplitude of smooth muscle is even smaller, and the pharmacological effect cannot be measured.
[0004] Therefore, there is a need for further improvement of the existing muscle strip fixing device.
[0005] The information disclosed in the background part of the present invention is only intended to increase the understanding of the overall background of the present invention, and should not be regarded as acknowledging or suggesting in any form that the information constitutes the prior art already known to a person skilled in the art. Summary of the invention
[0006] The utility model aims to provide a muscle strip fixing device, which can amplify the contraction amplitude of isolated muscle strips, and then adapt to receptor agonists that inhibit muscle strip contraction, thereby broadening the range of receptor agonist drugs that can be tested.
[0007] In order to solve the above problems, the utility model provides a muscle strip fixing device, comprising: a substrate; a first electrode and a second electrode, which are arranged on a first side of the substrate and along the length direction of the substrate; a first wire, which is electrically connected to the first electrode; and a second wire, which is electrically connected to the second electrode.
[0008] Preferably, the first electrode and the second electrode are both configured to be ring-shaped and have openings.
[0009] Preferably, the material of the first electrode and the second electrode is platinum.
[0010] Preferably, the distance between the first electrode and the second electrode ranges from 2.5 cm to 3.5 cm.
[0011] Preferably, the muscle strip fixing device further comprises: an oxygen tube, the bottom of which is fixed to the bottom of the second side of the base plate.
[0012] Preferably, the bottom of the oxygen tube has a bent portion so that the opening of the bottom of the oxygen tube faces upward.
[0013] Preferably, the substrate is provided with a plurality of through holes arranged along the length direction thereof.
[0014] Preferably, the first wire is disposed on the second side of the substrate, and the first wire is electrically connected to the first electrode after passing through one of the plurality of through holes.
[0015] Preferably, the second wire is disposed on the second side of the substrate, and the second wire is electrically connected to the second electrode after passing through one of the plurality of through holes.
[0016] Preferably, a clamping member is provided on the top of the base plate.
[0017] The muscle strip fixing device of the utility model forms an electric field by adding a first electrode and a second electrode, thereby amplifying the contraction amplitude of the isolated muscle strip, and adapting to receptor agonists that inhibit muscle strip contraction, thereby broadening the range of receptor agonist drugs that can be tested.
[0018] The device of the present invention has other characteristics and advantages, which will be obvious from the drawings and subsequent embodiments incorporated in this document, or will be described in detail in the drawings and subsequent embodiments incorporated in this document, which are used together to explain the specific principles of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 The structure of the muscle strip fixing device of the utility model is shown in FIG. Figure 1 ;
[0020] Figure 2 The structure of the muscle strip fixing device of the utility model is shown in FIG. Figure 2 ;
[0021] Figure 3 is a schematic structural diagram of a first electrode;
[0022] Figure 4 is a schematic structural diagram of the second electrode;
[0023] Figure 5 for Figure 2 A partial enlarged view of the center A;
[0024] Figure 6 for Figure 2 A partial enlarged view of point B in the middle;
[0025] Figure 7 It is a partial enlarged view of the clamping part;
[0026] Figure 8 To increase the concentration of morphine, the muscle tension test was recorded;
[0027] Fig. 9 is the IB-lg[Nx] curve with the addition of morphine;
[0028] Fig.10 To increase the concentration of naloxone, the muscle tension test was recorded;
[0029] Fig.11 This is the IB-lg[Nx] curve with the addition of naloxone.
[0030] Description of reference numerals:
[0031] 100: Baseboard 101: Hook
[0032] 102: Clamping piece 103: Through hole
[0033] 104: Opening
[0034] 200: first electrode 201: opening
[0035] 300: second electrode 301: opening
[0036] 400: first conductor 500: second conductor
[0037] 600: oxygen tube 601: bending part
[0038] 701: Isolated muscle strip 702: Connecting wire
[0039] 703: Connecting cable.
[0040] It should be understood that the drawings are not necessarily drawn to scale, but rather present simplified representations of various features to illustrate the basic principles of the present invention. The specific design features disclosed in the present invention (including, for example, specific dimensions, directions, positions, and shapes) will be determined in part by the specific intended application and use environment.
[0041] In the figures, like reference numbers refer to the same or equivalent parts of the present invention throughout the several figures of the drawing. DETAILED DESCRIPTION
[0042] Reference will be made in detail to various embodiments of the present invention, examples of which are presented in the accompanying drawings and described below. Although the present invention will be described in conjunction with exemplary embodiments, it should be understood that this specification is not intended to limit the present invention to these exemplary embodiments. On the contrary, the present invention is intended to cover not only these exemplary embodiments, but also various alternative forms, modified forms, equivalent forms and other embodiments that may be included in the spirit of the present invention and within the scope defined by the appended claims.
[0043] Figure 1 The structure of the muscle strip fixing device of the utility model is shown in FIG. Figure 1 ; Figure 2 The structure of the muscle strip fixing device of the utility model is shown in FIG. Figure 2 ; Figure 1 and Figure 2 The difference lies in the different observation angles; Figure 3 is a schematic structural diagram of a first electrode; Figure 4 is a schematic structural diagram of the second electrode; Figure 5 for Figure 2 A partial enlarged view of the center A; Figure 6 for Figure 2 A partial enlarged view of point B in the middle; Figure 7 It is a partial enlarged view of the clamping part; Figure 8 To increase the concentration of morphine, the muscle tension test was recorded; Fig. 9 is the IB-lg[Nx] curve with the addition of morphine; Fig.10 To increase the concentration of naloxone, the muscle tension test was recorded; Fig.11 This is the IB-lg[Nx] curve with the addition of naloxone.
[0044] Combine the following Figures 1 to 11 The muscle strip fixing device according to the embodiment of the utility model is described.
[0045] like Figure 1 and Figure 2 As shown, the muscle strip fixing device according to the embodiment of the present invention comprises: a substrate 100 , a first electrode 200 , a second electrode 300 , a first wire 400 and a second wire 500 .
[0046] The substrate 100 is used to carry the first electrode 200 , the second electrode 300 and the isolated muscle strip 701 .
[0047] The first electrode 200 and the second electrode 300 are disposed on a first side of the substrate 100 and along a length direction of the substrate 100 .
[0048] The first wire 400 is electrically connected to the first electrode 200 , and the second wire 500 is electrically connected to the second electrode 300 .
[0049] The first electrode 200 and the second electrode 300 are connected to a signal generator (the signal generator is not shown in the figure) through a first wire 400 and a second wire 500 .
[0050] The muscle strip fixing device of the utility model embodiment forms an electric field by adding a first electrode 200 and a second electrode 300, thereby amplifying the contraction amplitude of the isolated muscle strip 701, and adapting to receptor agonists that inhibit muscle strip contraction, thereby broadening the range of receptor agonist drugs that can be tested.
[0051] In an exemplary embodiment, the substrate 100 is generally plate-shaped. When in use, the substrate 100 is vertically arranged, and the bottom end of the substrate 100 is immersed in the bath. A hook 101 is arranged at the bottom of the first side of the substrate 100, and the first electrode 200 and the second electrode 300 are arranged on the first side of the substrate 100.
[0052] The first side of the substrate 100 is the side facing the user during use (ie, the front side), and the second side of the substrate 100 is the side facing away from the user during use (ie, the rear side).
[0053] In an exemplary embodiment, Figure 2 As shown, a clamping member 102 is disposed on the top of the second side of the substrate 100, and the clamping member 102 is formed as a whole with the substrate 100. Figure 7 As shown, the clamping member 102 is provided with an opening 104, and the first wire 400, the second wire 500 and the oxygen tube 600 introduced later can pass through the opening 104. The clamping member 102 can be clamped on the top of the bath to fix the substrate 100 in the bath. The first wire 400, the second wire 500 and the oxygen tube 600 introduced later may not pass through the opening 104 (see Figure 1 and Figure 2 ), or can pass through the opening 104 (not shown in the figure).
[0054] In an exemplary embodiment, the substrate 100 has a plurality of through holes 103 through which oxygen, which will be described later, can flow to the first side of the substrate 100. The plurality of through holes 103 are arranged along a length direction of the substrate 100.
[0055] In an exemplary embodiment, the first electrode 200 and the second electrode 300 are arranged on the first side of the substrate 100 and located above the hook 101. The first electrode 200 and the second electrode 300 are electrically connected to the signal generator through the first wire 400 and the second wire 500, respectively. After the signal generator outputs a repetitive square wave electrical signal (such as a voltage of 10 V, a wave width of 4 ms, and a main period of 10 s), a periodic weak electric field is formed between the first electrode 200 and the second electrode 300, and the contraction amplitude of the isolated muscle strip 701 can be increased under the stimulation of this electric field.
[0056] In an exemplary embodiment, Figure 3 As shown, the first electrode 200 is formed in a ring shape and has an opening 201 . The isolated muscle strip can be inserted into the first electrode 200 through the opening 201 , so as to facilitate the installation of the isolated muscle strip 701 .
[0057] In an exemplary embodiment, Figure 4 As shown, the second electrode 300 is formed in a ring shape and has an opening 301 . The isolated muscle strip can be inserted into the second electrode 300 through the opening 301 to facilitate the installation of the isolated muscle strip 701 .
[0058] In an exemplary embodiment, the material of the first electrode 200 and the second electrode 300 may be platinum wire.
[0059] In an exemplary embodiment, the distance d between the first electrode 200 and the second electrode 300 ranges from 2.5 cm to 3.5 cm, preferably 3 cm.
[0060] In an exemplary embodiment, the first electrode 200 and the second electrode 300 are preferably disposed at the middle and lower portion of the first side of the substrate 100, so that the volume of the nutrient solution required in the bath can be reduced. If the first electrode 200 and the second electrode 300 are disposed at the middle and upper portion of the first side of the substrate 100, more nutrient solution needs to be added to the bath to allow the isolated muscle strip to be completely immersed in the nutrient solution.
[0061] In an exemplary embodiment, Figure 1 and Figure 2 As shown, the first wire 400 is disposed on the second side of the substrate 100, and the first end of the first wire 400 passes through the substrate 100 in the thickness direction of the substrate 100 and is electrically connected to the first electrode 200. The first wire 400 and the first electrode 200 may be integrated or separated.
[0062] In an exemplary embodiment, Figure 5 As shown, the first wire 400 may pass through the through hole 103 of the substrate 100 to pass through the substrate 100 in the thickness direction of the substrate 100 .
[0063] In an exemplary embodiment, Figure 1 and Figure 2 As shown, the second wire 500 is disposed on the second side of the substrate 100, and the second end of the second wire 500 passes through the substrate 100 in the thickness direction of the substrate 100 and is electrically connected to the second electrode 300. The second wire 500 and the second electrode 300 may be integrated or separated.
[0064] In an exemplary embodiment, Figure 6As shown, the second wire 500 may pass through the through hole 103 of the substrate 100 to penetrate the substrate 100 in the thickness direction of the substrate 100 .
[0065] The first wire 400 and the second wire 500 both pass through the substrate 100 through the through hole 103. On the one hand, there is no need to drill holes for the first wire 400 and the second wire 500 separately during the manufacturing process, thereby reducing the manufacturing cost. On the other hand, the same substrate 100 is provided with a plurality of through holes 103 in its length direction. The through holes 103 that the first wire 400 and the second wire 500 need to pass through can be flexibly selected according to the different spacings between the first electrode 200 and the second electrode 300 in different experiments, thereby improving the versatility of the substrate 100.
[0066] The middle part of the first wire 400 and the middle part of the second wire 500 are wrapped together, which can improve the arrangement and management efficiency of the wires, ensure the neatness and beauty of the entire device, and optimize space utilization, improve maintainability and safety. The second end of the first wire 400 and the second end of the second wire 500 are electrically connected to the signal generator.
[0067] The main parts of the first conductive wire 400 and the second conductive wire 500 are both wrapped with an insulating layer.
[0068] In an exemplary embodiment, Figure 1 and Figure 2 As shown, the muscle strip fixing device according to the embodiment of the present invention further includes an oxygen tube 600 , and the bottom of the oxygen tube 600 is fixed to the bottom of the second side of the base plate 100 .
[0069] Oxygen enters the nutrient solution in the bath through the oxygen tube 600 to simulate the internal environment of the animal, thereby maintaining the activity of the isolated muscle strip 701. Figure 2 As shown, the bottom of the oxygen tube 600 has a bent portion 601, so that the bottom opening of the oxygen tube 600 is upward, so that oxygen can flow upward from the bottom of the nutrient solution as quickly as possible, thereby accelerating the oxygen supply speed.
[0070] The oxygen tube 600 is arranged on the second side of the substrate 100. After the oxygen in the oxygen tube 600 enters the nutrient solution in the bath, it can flow to the first side of the substrate 100 through the through hole 103 of the substrate 100 to speed up the oxygen supply to the isolated muscle strips located on the first side of the substrate 100.
[0071] The operation of the muscle strip fixing device according to the embodiment of the utility model will be described below with reference to the accompanying drawings.
[0072] Add nutrient solution to the bath.
[0073] One end of the isolated muscle strip 701 is connected to the hook 101 through the connecting line 702, the isolated muscle strip 701 passes through the second electrode 300 and the first electrode 200, and the other end of the isolated muscle strip 701 is connected to the tension transducer through the connecting line 703. Specifically, the isolated muscle strip 701 can pass through the first electrode 200 and the second electrode 300 from top to bottom, and then one end of the isolated muscle strip 701 is connected to the hook 101, or one end of the isolated muscle strip 701 is first connected to the hook 101, and then passes through the second electrode 300 and the first electrode 200 from bottom to top, or one end of the isolated muscle strip 701 is first connected to the hook 101, and then inserted into the second electrode 300 from the opening 301, and then inserted into the first electrode 200 from the opening 201.
[0074] The substrate 100 is placed vertically into the bath, and the substrate 100 is clamped in the bath using the clamping member 102 .
[0075] If the receptor agonist is a drug that can promote muscle strip contraction, the pharmacological effect can be measured without electrical stimulation.
[0076] If the receptor agonist is a drug that can inhibit the contraction of the muscle strip, the first wire 400 and the second wire 500 are connected to the signal generator, and electrical stimulation is applied to measure the pharmacological effect. Specifically, when the signal generator outputs repeated square wave electrical stimulation (such as voltage 10 v, wave width 4 ms, main period 10 s), a periodic weak electric field is formed between the first electrode 200 and the second electrode 300. Under this electric field stimulation, the contraction amplitude of the isolated muscle strip 701 increases. The voltage of the square wave electrical stimulation is adjusted to find the minimum voltage that can cause the largest contraction amplitude of the isolated muscle strip 701 as the background stimulation intensity. At this time, the contraction amplitude of the isolated muscle strip 701 is already large due to the electric field stimulation without the effect of the drug, which is easy to measure. If a receptor agonist that inhibits the contraction of the muscle strip is added, although the contraction amplitude becomes smaller, it is still easy to measure accurately. Therefore, it can be used to study the affinity index of the receptor agonist with inhibitory effect and the determination experiment of the pA2 value of the antagonistic effect of the antagonist.
[0077] Determination experiment:
[0078] Experimental steps:
[0079] 1. Making muscle strips:
[0080] a) After killing the guinea pig by hitting its head, remove the ileum. Cut the ileum 6 cm below the pylorus, or find the ileocecal part, cut the intestine about 3 cm above the ileocecal part, and remove a section of the ileum. Take a 3 cm section of the intestine and place it in a culture dish filled with Krebs solution.
[0081] b) Cut off the mesentery, peel off the external longitudinal muscle, tie one end of it to the small hook of the electrode, and connect the other end to the arm of the tension transducer with a silk thread. Place the electrode in the smooth muscle bath.
[0082] 2. Select the observation index: connect the tension transducer (channel 1), open the software, experimental module - pharmacology - effect of drugs on isolated intestine.
[0083] 3. Initial conditions of muscle strips: 19 ml of Krebs' solution in the bath, oxygen (5% CO2, 95% O2), 37°C, net load of 0.2-0.3 g. After the muscle strips were balanced for about 0.5 h, repeated square wave electrical stimulation (fine voltage, single stimulation, continuous, non-programmed, wave width 4 ms, frequency 0.1 Hz, intensity 15 V, repetition number +∞) was given, and the contraction of the muscle strips was recorded.
[0084] 4. Mor's IC 50 Determination (cumulative dosing method):
[0085] a) Record the contraction of the muscle strip before drug administration, and add Mor after the contraction stabilizes; each time the drug is added, wait until the contraction stabilizes before giving the next dose of drug, until E max (Until the inhibitory effect no longer increases).
[0086] b) Stop electrical stimulation and wash with Krebs solution, first several times in quick succession, then wash every few minutes, for a total of about 15 times.
[0087] c) Re-apply electrical stimulation and record the contraction of the muscle strip for another 2 minutes. When the contraction is stable, use the muscle strip for the next test.
[0088] 5. Determination of Nal pA2 value (cumulative administration method):
[0089] a) Determine X: 2X = 70% E max , calculate X = 20μl 10 -4 mol / L Mor.
[0090] b) Dosing: Add X dose of Mor twice; after the contraction stabilizes, add Nal and record the contraction of the muscle strip after the second dosing.
[0091] Experimental Results
[0092] (1) IC 50 Determination of.
[0093]
[0094] The muscle tension test records of increasing morphine concentrations are shown in Table 1. Figure 8 shown.
[0095] According to the amount of morphine added each time, the cumulative morphine concentration (M) in the system is Figure 8 Read the data after the muscle tension stabilizes after each drug addition, calculate the degree of inhibition (E) of the muscle strips by the drug in the system after each drug addition, calculate the ratio p of E to the maximum degree of drug inhibition Emax, and make Curve (see Fig. 9 ), and the fitting curve y=1.0989x+6.661 is obtained. Substitute the curve and calculate lgIC 50 The value is about -6.06, and the IC50 value is about 8.68×10 -7 .
[0096] (2) Determination of pA2
[0097]
[0098] After the first addition of morphine, the contractile tension of the muscle strip should drop to 70% of Emax. According to the IC50 data, in the experiment of measuring pA2, morphine should be added to the bath twice in succession, with 20 μL of 1x10 -4 mmol / L morphine solution, the results are shown in Table 2.
[0099] After the second addition of morphine, wait until the contraction tension of the muscle strip is stable, and add 10 μL, 30 μL, 40 μL, 50 μL, 50 μL, and 50 μL of morphine to the bath in the order of 10 μL, 20 μL, 30 μL, 40 μL, 50 μL, and 50 μL. -6 mmol / L concentration of naloxone solution, the results are shown in Table 3, the process is as follows Fig.10 As shown. Since the second set of data deviates too much, this set of data is discarded, and the third to seventh sets of data are used to draw a graph to obtain Fig.11 .
[0100] After linear fitting of the data, the fitting curve is y=-1.8085x-14.087, R 2 =0.9492. Substituting y=1, we get x=-8.342, that is, the pA2 of naloxone antagonizing morphine is 8.342, and the concentration of naloxone is 4.55x10 -9 mmol / L.
[0101]
[0102] Process Analysis
[0103] 1. When morphine was added, it was observed that the contraction amplitude of the muscle strips increased after the addition of the drug, and then returned to normal or gradually decreased. The reason for this was that the muscle strips used in this experiment were more active and more sensitive to local high-concentration drug stimulation, and then gradually adapted.
[0104] 2. Before adding morphine in step 1, the maximum contraction amplitude of the muscle strips after stabilization was 1.40g, while before adding naloxone in step 2, the contraction amplitude of the muscle strips was slightly greater than 1.40g, and even 1.49g contraction occurred later. The reason for this is that the muscle strips used in this group have strong adaptability and always maintain a high contraction capacity.
[0105] Data processing
[0106] When analyzing the contraction tension of the muscle strip in step 2, theoretically, (absolute value of muscle strip contraction - minimum value of the corresponding wave) should be used to more accurately reflect the contraction amplitude and reduce the systematic error caused by factors such as bubbles and shaking. However, since the minimum tension value was relatively stable in this group of experiments, only the absolute value of muscle strip contraction was used in the calculation.
[0107] Results and Discussion
[0108] 1. IC 50 The correlation coefficients of the fitting curves in the pA2 determination process were high, indicating that the results were more reliable.
[0109] 2. The pA2 value calculated in this experiment is 8.342, and the theoretical value obtained by query is 8.37, with a relative error of e=0.0032, which is an ideal result.
[0110] from Figure 8 and Fig.10 It can be seen that although receptor agonists are drugs that can inhibit muscle strip contraction, under the stimulation of the electric field, the contraction amplitude of the isolated muscle strip becomes larger, which is still easy to measure accurately.
[0111] For convenience of interpretation and precise definition of the appended claims, the terms "upper", "lower", "inner", "outer", "above", "below", "upper", "lower", "upward", "downward", "front", "back", "back", "inside", "outside", "inward", "outward", "interior", "exterior", "inner", "external", "forward", and "backward" are used to describe the features of the exemplary embodiments with reference to the positions of such features as shown in the accompanying drawings.
[0112] The description presented above of the specific exemplary embodiments of the utility model is for the purpose of illustration and description. The foregoing description is not intended to be exhaustive, nor is it intended to limit the utility model to the precise form disclosed, and it is clear that many changes and variations are possible based on the above teachings. The exemplary embodiments are selected and described in order to explain the specific principles of the utility model and its practical application, so that other technicians in the field can realize and utilize the various exemplary embodiments of the utility model and its different selected forms and modified forms. The scope of the utility model is defined by the attached claims and their equivalents.
Claims
1. A muscle strip fixing device, characterized in that: include: substrate; A first electrode and a second electrode, which are arranged on a first side of the substrate and along a length direction of the substrate; a first lead electrically connected to the first electrode; as well as A second wire is electrically connected to the second electrode.
2. The muscle strip fixing device according to claim 1, characterized in that: The first electrode and the second electrode are both configured in a ring shape and have an opening.
3. The muscle strip fixing device according to claim 1, characterized in that: The first electrode and the second electrode are made of platinum.
4. The muscle strip fixing device according to claim 1, characterized in that: The distance between the first electrode and the second electrode ranges from 2.5 cm to 3.5 cm.
5. The muscle strip fixing device according to claim 4, characterized in that: It further includes: The bottom of the oxygen tube is fixed to the bottom of the second side of the base plate.
6. The muscle strip fixing device according to claim 5, characterized in that: The bottom of the oxygen tube has a bent portion so that the opening of the bottom of the oxygen tube faces upward.
7. The muscle strip fixing device according to claim 5, characterized in that: The substrate is provided with a plurality of through holes arranged along the length direction thereof.
8. The muscle strip fixing device according to claim 7, characterized in that: The first wire is disposed on the second side of the substrate, and the first wire is electrically connected to the first electrode after passing through one of the plurality of through holes.
9. The muscle strip fixing device according to claim 7, characterized in that: The second wire is disposed on the second side of the substrate, and the second wire is electrically connected to the second electrode after passing through one of the plurality of through holes.
10. The muscle strip fixing device according to claim 1, characterized in that: The top of the base plate has a clamping member.