Light sheet scanning implementation system based on double cylindrical mirrors

Abstract

The utility model discloses an optical sheet scanning realization system based on double cylindrical mirrors, comprising a beam splitter prism used for receiving parallel light beams and reflecting the parallel light beams; the first cylindrical mirror is arranged on one side of the beam splitter prism and used for receiving the parallel light beams reflected by the beam splitter prism and forming a light sheet; the objective lens I is arranged on one side, opposite to the beam splitter prism, of the cylindrical mirror I and is used for receiving and converging the light sheet emitted by the cylindrical mirror I; the scanning reflector is arranged on one side of the objective lens I and used for reflecting the light sheet emitted by the objective lens I, and the scanning reflector can oscillate back and forth in the axial direction; the second cylindrical mirror is arranged on the other side, opposite to the first cylindrical mirror, of the beam splitter prism and used for focusing and shaping the light sheet which is reflected by the scanning reflector and then transmitted to the beam splitter prism through the first objective lens and the first cylindrical mirror. The X-ray plate has the advantages that the structure is simple, and the formed X-ray plate can be scanned in the axial direction.

Description

Technical Field

[0001] The utility model relates to the technical field of light sheet scanning, in particular to a light sheet scanning realization system based on double cylindrical mirrors. Background Art

[0002] With the development of fluorescence microscopy technology, optical imaging and scanning technology is increasingly being used in the fields of medical treatment, scientific research, industrial inspection, etc. In applications using fluorescence microscopy technology for imaging and scanning, a light sheet is usually emitted by a light source to illuminate the sample, and then the sample is moved to achieve scanning imaging of the sample. That is, the illumination light sheet in the existing light sheet fluorescence microscopy system cannot achieve the scanning function in the axial direction, which limits its application.

[0003] In view of this, the purpose of the utility model is to provide a new technical solution to solve the existing technical problems. Utility Model Content

[0004] In order to overcome the deficiencies of the prior art, the utility model provides a light sheet scanning realization system based on a double cylindrical mirror, which solves the problem that the illumination light sheet of the existing light sheet fluorescence microscopy system cannot realize three-dimensional scanning.

[0005] The technical solution adopted by the utility model to solve its technical problems is:

[0006] A light sheet scanning realization system based on a double cylindrical mirror, comprising:

[0007] A beam splitter prism, the beam splitter prism is used to receive a parallel light beam and reflect the parallel light beam;

[0008] A cylindrical mirror 1, which is disposed on one side of the beam splitter prism and is used to receive the parallel light beam reflected by the beam splitter prism and form a light sheet;

[0009] Objective lens 1, which is disposed on a side of cylindrical lens 1 facing away from the beam splitter prism, and is used to receive and converge the light sheet emitted by cylindrical lens 1;

[0010] A scanning reflector, which is disposed on one side of the first objective lens and is used to reflect the light sheet emitted from the first objective lens, and the scanning reflector can be oscillated back and forth in the axial direction;

[0011] The cylindrical mirror 2 is arranged on the other side of the beam splitter prism relative to the cylindrical mirror 1, and is used to focus and shape the light sheet that is reflected by the scanning reflector and then transmitted to the beam splitter prism through the objective lens 1 and the cylindrical mirror 1 again.

[0012] In the above structure, it also includes a second objective lens, which is arranged on the side of the second cylindrical lens facing away from the beam splitter prism.

[0013] In the above structure, a cage cube is also included, and the beam splitter prism is installed in the cage cube.

[0014] In the above structure, two threaded cage plates are also included, and the cylindrical mirror 1 and the cylindrical mirror 2 are respectively installed in the corresponding threaded cage plates.

[0015] In the above structure, mounting rods are installed between the first objective lens and the adjacent threaded cage plate, between the cage cube and the adjacent threaded cage plate, and between the second objective lens and the adjacent threaded cage plate.

[0016] In the above structure, an exciter is also included, and the scanning reflection mirror is arranged on the exciter.

[0017] In the above structure, the light sheet emitted from the first objective lens to the scanning reflector is symmetrical to the light sheet surface formed by the second objective lens.

[0018] The beneficial effects of the utility model are as follows: a symmetrical light path is formed by arranging two cylindrical mirrors in the light path, and a scanning reflector capable of axial scanning is arranged, so that the light sheet surface formed by the light path system can change position along with the axial scanning of the scanning reflector, thereby realizing the scanning of the light sheet, and the structure is simple, the imaging quality of the light sheet formed is high, and the stability of the light path system is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0020] Figure 1 It is a schematic diagram of the principle of an embodiment of the utility model;

[0021] Figure 2 It is a schematic diagram of the principle of another embodiment of the utility model;

[0022] Figure 3 It is a schematic diagram of the assembly structure of an embodiment of the utility model.

[0023] Figure numerals: 1. beam splitter; 2. cylindrical mirror 1; 3. objective lens 1; 4. scanning reflector; 5. cylindrical mirror 2; 6. objective lens 2; 62. light sheet surface; 7. exciter; 8. cage cube; 9. threaded cage plate; 10. mounting rod. DETAILED DESCRIPTION

[0024] The following is combined with Figure 1-3 The utility model is further described.

[0025] The following will clearly and completely describe the concept, specific structure and technical effects of the utility model in combination with the embodiments and drawings, so as to fully understand the purpose, characteristics and effects of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the utility model. In addition, all the connection / connection relationships involved in the patent do not refer to the direct connection of components, but refer to the formation of a better connection structure by adding or reducing connection accessories according to the specific implementation situation. The various technical features in the creation of the utility model can be combined interchangeably without conflicting with each other.

[0026] Reference Figures 1 to 3 The utility model provides a light sheet scanning realization system based on double cylindrical mirrors, which shapes the incident parallel light beam to output the light sheet by arranging two cylindrical mirrors in the optical path system, and changes the position of the light sheet surface 62 by arranging an axially movable scanning reflector 4, thereby realizing the scanning of the light sheet. The light sheet scanning realization system based on double cylindrical mirrors includes a beam splitter prism 1, a cylindrical mirror 2, an objective lens 3, a scanning reflector 4 and a cylindrical mirror 5, wherein the beam splitter prism 1 is used to receive a parallel light beam, and reflect the parallel light beam to guide the parallel light beam into the cylindrical mirror 2; the cylindrical mirror 2 is used to receive and focus the parallel light beam reflected by the beam splitter prism 1 to form a light sheet; the objective lens 3 is used to receive and converge the light sheet emitted by the cylindrical mirror 2; the scanning reflector 4 is used to reflect the light sheet emitted by the objective lens 3, and the scanning reflector 4 can oscillate back and forth along the axial direction of the light path and is set in the light path system; the cylindrical mirror 5 is used to further focus and shape the light sheet emitted by the scanning reflector 4, so that the system outputs a high-quality illumination light sheet and forms an accurate and clear image on the imaging light sheet surface 62.

[0027] Reference Figure 1 Specifically, the cylindrical mirror 2 is arranged on one side of the reflection light path of the beam splitter prism 1. When the parallel light beam enters the beam splitter prism 1, it is reflected by the beam splitter prism 1 and guided to the cylindrical mirror 2. The cylindrical mirror is a kind of aspherical mirror, and has a specific refractive power in a certain direction, but has no refractive power in another direction perpendicular to this direction. When a beam of parallel light enters the cylindrical mirror from the direction in which the cylindrical mirror has a specific refractive power, the cylindrical mirror can diverge the light, for example, convert a point light spot into a line light spot. According to the optical characteristics of the cylindrical mirror, the parallel light beam emitted from the cylindrical mirror 2 will be diverged by the cylindrical mirror 2 and converted into a linear light source (i.e., forming a light sheet).

[0028] Objective lens 13 is arranged on the side of cylindrical mirror 12 facing away from beam splitter prism 1, that is, objective lens 13 is used to receive the light sheet output by cylindrical mirror 12, and converge and shape the light sheet to improve the illumination quality of the light sheet. Scanning reflector 4 is arranged on the side of the light emitting surface of objective lens 13. Objective lens 13 guides the illumination light beam from scanning reflector 4. Scanning reflector 4 is used to change the transmission direction of the light path so that the light sheet is reflected and enters objective lens 13 again. At this time, the light sheet is transmitted along the opposite path to the original one. After being focused by objective lens 13, the light sheet enters cylindrical mirror 2 and beam splitter prism 1 in turn. Under the action of beam splitter prism 1 again, the light sheet is guided to cylindrical mirror 25. Cylindrical mirror 25 further diverges and shapes the light sheet so that the light forms a light sheet with better quality.

[0029] Reference Figure 2 and Figure 3 Furthermore, the light sheet scanning realization system based on the double cylindrical mirrors also includes an objective lens 2 6, which is arranged on the other side of the cylindrical mirror 1 2 relative to the beam splitter prism 1. The light sheet emitted from the cylindrical mirror 2 5 is received by the objective lens 2 6, and the objective lens 2 6 further shapes and focuses the light sheet to further improve the illumination quality of the light sheet. Setting the objective lens 2 6 to further shape and focus the light sheet is conducive to finally outputting a clear and accurate illumination light sheet. The illumination light sheet output from the objective lens 2 6 is finally transmitted to the light sheet surface 62.

[0030] Furthermore, by adjusting the relative positions of the cylindrical mirror 1 2 and the cylindrical mirror 2 5, a symmetrical optical path is formed on both sides of the cylindrical mirror 1 2 and the cylindrical mirror 2 5, that is, the light sheet emitted from the objective lens 1 3 to the scanning reflector 4 and the light sheet surface 62 formed by the emission from the objective lens 2 6 are in a symmetrical relationship. The light sheet scanning realization system based on the double cylindrical mirror is also provided with an exciter 7, and the scanning reflector 4 is fixedly arranged on the exciter 7. The exciter 7 can drive the scanning reflector 4 to oscillate back and forth in the axial direction of the optical path. Since the light sheet surface 62 formed by the emission from the objective lens 2 6 is in a symmetrical relationship with it, as the scanning reflector 4 oscillates back and forth in the axial direction, the position of the light sheet surface 62 can be changed, thereby realizing the scanning of the light sheet.

[0031] Furthermore, in one embodiment, the light sheet scanning realization system based on the double cylindrical mirror is provided with a cage cube 8 and two threaded cage plates 9, wherein the beam splitter prism 1 is installed in the cage cube 8, and the cylindrical mirror 1 2 and the cylindrical mirror 2 5 are installed in the corresponding threaded cage plates 9 respectively. Moreover, mounting connecting rods 10 are installed between the objective lens 1 3 and the adjacent threaded cage plates 9, between the cage cube 8 and the adjacent threaded cage plates 9, and between the objective lens 2 6 and the adjacent threaded cage plates 9, that is, the two sides of the cage cube 8 equipped with the beam splitter prism 1 are respectively connected and fixed to the threaded cage plate 9 equipped with the cylindrical mirror 1 2 and the threaded cage plate 9 equipped with the cylindrical mirror 2 5 through a plurality of mounting connecting rods 10; the threaded cage plate 9 equipped with the cylindrical mirror 1 2 is also connected and fixed to the objective lens 1 3 through the mounting connecting rods 10; the threaded cage plate 9 equipped with the cylindrical mirror 2 5 and the objective lens 2 6 are also connected and fixed through the mounting connecting rods 10. The optical devices are connected and fixed by the cage cube 8, the threaded cage plate 9 and the mounting rod 10, making the entire system more compact, which is beneficial to improving the stability and reliability of the entire optical system, and is also beneficial to ensuring the high alignment accuracy of the optical axes of all optical devices and ensuring the imaging quality. It should be noted that in other embodiments, the connection and fixation between the optical devices can also adopt other connection structures, which are not limited here.

[0032] The above is a specific description of the preferred implementation of the utility model, but the invention of the utility model is not limited to the described embodiments. Technical personnel familiar with the field can also make various equivalent deformations or substitutions without violating the spirit of the utility model. These equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A light sheet scanning realization system based on a double cylindrical mirror, characterized in that: include: A beam splitter prism, the beam splitter prism is used to receive a parallel light beam and reflect the parallel light beam; A cylindrical mirror 1, which is disposed on one side of the beam splitter prism and is used to receive the parallel light beam reflected by the beam splitter prism and form a light sheet; Objective lens 1, which is disposed on a side of cylindrical lens 1 facing away from the beam splitter prism, and is used to receive and converge the light sheet emitted by cylindrical lens 1; A scanning reflector, which is disposed on one side of the first objective lens and is used to reflect the light sheet emitted from the first objective lens, and the scanning reflector can be oscillated back and forth in the axial direction; The cylindrical mirror 2 is arranged on the other side of the beam splitter prism relative to the cylindrical mirror 1, and is used to focus and shape the light sheet that is reflected by the scanning reflector and then transmitted to the beam splitter prism through the objective lens 1 and the cylindrical mirror 1 again.

2. The light sheet scanning realization system based on double cylindrical mirrors according to claim 1, characterized in that: It also includes a second objective lens, which is arranged on the side of the second cylindrical lens facing away from the beam splitter prism.

3. The light sheet scanning realization system based on double cylindrical mirrors according to claim 2, characterized in that: Also included is a cage cube in which the beam splitter prism is mounted.

4. The light sheet scanning realization system based on double cylindrical mirrors according to claim 3, characterized in that: It also includes two threaded cage plates, and the cylindrical mirror 1 and the cylindrical mirror 2 are respectively installed in the corresponding threaded cage plates.

5. The light sheet scanning realization system based on double cylindrical mirrors according to claim 4, characterized in that: Mounting rods are installed between the first objective lens and the adjacent threaded cage plate, between the cage cube and the adjacent threaded cage plate, and between the second objective lens and the adjacent threaded cage plate.

6. The light sheet scanning realization system based on double cylindrical mirrors according to claim 1, characterized in that: It also includes an exciter, and the scanning reflector is arranged on the exciter.

7. The light sheet scanning realization system based on double cylindrical mirrors according to claim 2, characterized in that: The light sheet emitted from the first objective lens to the scanning reflector is symmetrical to the light sheet surface formed by the second objective lens.

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