Sample pretreatment device for monoclonal antibody preparation

Abstract

The sample pretreatment device comprises a tank body, a cover plate is arranged at the opening end of the tank body through a clamping assembly, lifting rings are symmetrically arranged on the cover plate, a rotating plate is rotationally arranged in the middle of the cover plate, plugging assemblies are arranged below the rotating plate at equal intervals, and a rotating centrifugal mechanism is arranged in the tank body. Collecting barrels are arranged in the rotating centrifugal mechanism at equal intervals and provided with filtering barrels through positioning assemblies, discharging assemblies are arranged at the bottom ends of the collecting barrels, and supporting legs are arranged at the bottom end of the tank body at equal intervals. According to the utility model, a plurality of batches of samples can be centrifugally filtered at the same time, the treatment efficiency is improved, meanwhile, the filtered samples can be independently discharged and collected in cooperation with the discharging assembly, the subsequent preparation and use are facilitated, and the collecting cylinder and the filtering cylinder can be detachable through the arranged positioning assembly, so that the cost is reduced. The filter cartridge and the collecting cartridge can be conveniently cleaned, so that the cleanliness is ensured, and the next filtering use is not influenced.

Description

Technical Field

[0001] The utility model relates to the technical field of sample processing, in particular to a sample preprocessing device for preparing monoclonal antibodies. Background Art

[0002] Before preparing monoclonal antibodies, the samples to be used need to be pretreated to eliminate interfering factors, completely retain the components to be tested, and concentrate the components to be tested in order to obtain reliable analysis. Pretreatment usually includes filtration, heating and other processes.

[0003] However, the existing pre-treatment devices have certain defects when filtering samples. Most of them can only filter a batch of samples, and cannot filter multiple groups of samples at the same time. In addition, impurities remain inside the device after use, which is inconvenient to clean and easily leads to impurity residues, affecting the next filtration use. Utility Model Content

[0004] The purpose of the utility model is to provide a sample pretreatment device for preparing monoclonal antibodies to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the utility model provides the following technical solutions: a sample pretreatment device for preparing monoclonal antibodies, comprising a tank body, a cover plate is provided at the open end of the tank body through a snap-fit ​​assembly, lifting rings are symmetrically provided on the cover plate, a rotating plate is rotatably provided in the middle of the cover plate, a blocking assembly is equidistantly provided under the rotating plate, a rotating centrifugal mechanism is provided in the tank body, a collecting cylinder is equidistantly provided in the rotating centrifugal mechanism, a filtering cylinder is provided in the collecting cylinder through a positioning assembly, a discharge assembly is provided at the bottom of the collecting cylinder, and supporting feet are equidistantly provided at the bottom of the tank body.

[0006] Preferably, in order to facilitate the rotation of the rotating cylinder, thereby facilitating centrifugal filtration of the sample and accelerating the filtration efficiency, the rotating centrifugal mechanism includes a rotating cylinder rotatably arranged in the tank body, and the middle of the bottom end of the rotating cylinder is fixedly connected to the motor output end.

[0007] Preferably, in order to ensure stability during the rotation process, the rotating centrifugal mechanism further includes guide blocks equidistantly arranged on the circumferential side of the rotating cylinder, and the inner wall of the tank body is provided with guide grooves matching the guide blocks.

[0008] Preferably, in order to facilitate the assembly and disassembly of the cover plate, facilitate use, and subsequent removal for cleaning of the interior, the locking assembly includes a buckle body equidistantly arranged on the side surface of the tank body, and tower hooks matching the buckle body are equidistantly arranged on the side surface of the cover plate.

[0009] Preferably, in order to facilitate installation and disassembly, both are detachable to facilitate cleaning operations after subsequent use. The positioning assembly includes a card plate arranged on the top of the filter cylinder, and the inner wall of the collection cylinder is provided with a card groove that engages with the card plate.

[0010] Preferably, in order to facilitate the collection of filtered samples and subsequent preparation and use, the discharge assembly includes a collection tube arranged at the bottom end of the collection cylinder, and the collection tube passes through the rotating cylinder and extends to the bottom where a valve is provided.

[0011] Preferably, in order to facilitate sealing of the filter cartridge during centrifugation to prevent the sample from being thrown out, the sealing assembly includes a pressure plate equidistantly arranged under the rotating plate, a sealing plug matching the filter cartridge is provided under the pressure plate, and a sealing ring is provided on the side surface of the sealing plug.

[0012] Compared with the prior art, the beneficial effects of the utility model are:

[0013] (1) Through the multiple sets of collecting cylinders and filter cylinders, multiple batches of samples can be centrifugally filtered at the same time to improve the processing efficiency. At the same time, the filtered samples can be discharged and collected separately in cooperation with the discharge assembly, which is convenient for subsequent preparation and use. The positioning assembly allows the collecting cylinder and the filter cylinder to be detachable, and can be easily disassembled after use, which is convenient for cleaning the filter cylinder and the collecting cylinder to ensure cleanliness and not affect the next filtration use;

[0014] (2) The rotating centrifugal mechanism can be used to rotate the centrifuge during filtration, which can speed up the efficiency of impurity filtration and shorten the time required, while ensuring stability during the rotation process. The sealing component can be used to seal the top of the filter cartridge during centrifugal rotation, thereby ensuring that the sample is not thrown out during the filtration process. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the structure of a sample pretreatment device for preparing monoclonal antibodies proposed by the utility model;

[0016] Figure 2 This is a schematic diagram of the structure of a tank in a sample pretreatment device for preparing monoclonal antibodies proposed in the utility model;

[0017] Figure 3 This is a structural schematic diagram of a cover plate in a sample pretreatment device for preparing monoclonal antibodies proposed by the utility model;

[0018] Figure 4 A partial structural schematic diagram of a sample pretreatment device for preparing monoclonal antibodies proposed by the utility model;

[0019] Figure 5The utility model discloses a schematic diagram of the structure of a collection cartridge and a filter cartridge in a sample pretreatment device for preparing monoclonal antibodies.

[0020] In the figure: 1. tank body; 2. cover plate; 3. rotating plate; 4. rotating cylinder; 5. lifting ring; 6. motor; 7. support foot; 8. buckle body; 9. tower hook; 10. guide groove; 11. guide block; 12. collecting cylinder; 13. pressure plate; 14. sealing plug; 15. sealing ring; 16. collecting tube; 17. valve; 18. slot; 19. clamping plate; 20. filter cylinder. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0022] See also Figure 1-Figure 5 The utility model provides an embodiment: a sample pretreatment device for preparing monoclonal antibodies, comprising a tank body 1 for filtering samples during the preparation of monoclonal antibodies. In order to ensure its stability, legs 7 (the number can be three groups) are equidistantly welded vertically at the bottom to ensure stability and firmness. Buckle bodies 8 of the locking components are equidistantly installed on the side surfaces around the top of the tank body 1, and tower hooks 9 are equidistantly installed on the side surfaces around the cover plate 2. The two constitute tower buckles, which can be four groups, which can be conveniently installed and fixed with the cover plate 2. Two groups of lifting rings 5 ​​are symmetrically welded on the top of the cover plate 2 to facilitate the placement and removal of the cover plate 2.

[0023] See also Figure 1-Figure 5 In order to facilitate the filtering process, improve the filtering effect, and remove impurities such as residues in the sample, a rotating centrifugal mechanism is arranged inside the tank body 1. The rotating centrifugal mechanism includes a rotating cylinder 4 rotatably connected to the tank body 1 (they can be rotatably connected through a bearing), and the middle part of the bottom end of the rotating cylinder 4 is fixedly connected to the output end of the motor 6 through a coupling, and the motor 6 can be fixed to the bottom of the tank body 1 or the support foot 7 through a fixing device (such as a fixing ring and a fixing rod, etc., not shown in the figure, which is a relatively mature technology and will not be described here) (fixed by screws or welding). The fixing is selected according to the situation, which can be convenient for the motor 6 to work and can rotate the rotating cylinder 4 in the tank body 1. In order to ensure the stability of the rotation, guide blocks 11 are equidistantly welded on the side surfaces (the top end position) of the rotating cylinder 4, and the inner wall of the tank body 1 is provided with a guide groove 10 in which the guide block 11 slides, thereby ensuring the stability of the subsequent rotating cylinder 4 during the rotation process.

[0024] See also Figure 1-Figure 5In order to process multiple batches of samples separately at the same time, and to facilitate the collection of filtered sample liquid, and to facilitate the cleaning operation after subsequent use to ensure the cleanliness of the interior, multiple groups of collecting cylinders 12 are equidistantly arranged inside the rotating cylinder 4, and the number can be four or five groups, etc., and a collecting pipe 16 of a discharge assembly is arranged at the bottom of the collecting cylinder 12, which penetrates the rotating cylinder 4 and extends to the bottom to be installed with a valve 17 (which can be an electric control valve or a manual valve, which can be set according to the actual situation), in order to enable centrifugal filtration, and to clean up impurities after filtration. A card slot 18 of a positioning component is provided at the top of the inner wall of the collecting cylinder 12, and a card plate 19 adapted to the card slot 18 is provided at the top of the filter cylinder 20, which can be easily assembled to prevent the sample liquid from spilling during rotation and centrifugation. A rotating plate 3 (rotatably connected through a bearing) is rotated in the middle of the cover plate 2, and a pressure plate 13 of a blocking component (which corresponds to the collecting cylinder 12 one by one) is equidistantly arranged under the rotating plate 3, and a sealing plug 14 is bonded under the pressure plate 13 to seal the top of the filter cylinder 20. In order to ensure the sealing effect, a sealing ring 15 is sleeved on the surrounding side of the sealing plug 14.

[0025] Working principle: When the utility model is in use, when assembling the filter cartridge 20, insert it into the collection cartridge 12, connect it with the card plate 19 and the card slot 18, and then add multiple batches of samples to be filtered into the filter cartridge 20 respectively, and then cover the cover 2, and the sealing plug 14 is correspondingly plugged with the filter cartridge 20, and the sealing effect is ensured by the sealing ring 15, the pressing plate 13 contacts the top of the collection cartridge 12 and presses it, and then fastens it with the buckle body 8 and the tower hook 9 to complete the fixation of the cover 2, and then the motor 6 rotates, driving the rotating cylinder 4 to rotate in the tank body 1, and at the same time the rotating plate 3 rotates in the cover 2 The filter cartridge 20 is in motion and can filter the sample under the action of the rotating centrifugal force. The impurities remain in the filter cartridge 20, and the filtered sample flows into the collection cartridge 12. After the filtration is completed, the sample can be collected through the collection tube 16 by opening the valve 17. When cleaning the filter cartridge 20 and the collection cartridge 12, the cover plate 2 is lost by pulling down the buckle body 8, and the cover plate 2 is removed by lifting the lifting ring 5 upwards. At the same time, the sealing plug 14 leaves the filter cartridge 20, so that the filter cartridge 20 can be easily taken out, the internal impurities can be easily cleaned, and the collection cartridge 12 can be easily cleaned for the next use.

[0026] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention. Any reference numeral in a claim should not be regarded as limiting the claim to which it relates.

Claims

1. A sample pretreatment device for preparing monoclonal antibodies, comprising a tank (1), characterized in that: The open end of the tank body (1) is provided with a cover plate (2) through a snap-fit ​​assembly, and a lifting ring (5) is symmetrically provided on the cover plate (2). A rotating plate (3) is rotatably provided in the middle of the cover plate (2), and a blocking assembly is equidistantly provided below the rotating plate (3). A rotating centrifugal mechanism is provided in the tank body (1), and a collecting cylinder (12) is equidistantly provided in the rotating centrifugal mechanism. The collecting cylinder (12) is provided with a filter cylinder (20) through a positioning assembly, and a discharge assembly is provided at the bottom end of the collecting cylinder (12). Support feet (7) are equidistantly provided at the bottom end of the tank body (1).

2. A sample pretreatment device for preparing monoclonal antibodies according to claim 1, characterized in that: The rotating centrifugal mechanism comprises a rotating cylinder (4) rotatably arranged in a tank body (1), and the middle part of the bottom end of the rotating cylinder (4) is fixedly connected to the output end of a motor (6).

3. A sample pretreatment device for preparing monoclonal antibodies according to claim 2, characterized in that: The rotating centrifugal mechanism further comprises guide blocks (11) arranged at equal distances on the peripheral side of the rotating cylinder (4), and the inner wall of the tank body (1) is provided with guide grooves (10) matching the guide blocks (11).

4. The sample pretreatment device for preparing monoclonal antibodies according to claim 3, characterized in that: The snap-fit ​​assembly comprises buckle bodies (8) equidistantly arranged on the peripheral side of the tank body (1), and tower hooks (9) matching the buckle bodies (8) are equidistantly arranged on the peripheral side of the cover plate (2).

5. The sample pretreatment device for preparing monoclonal antibodies according to claim 4, characterized in that: The positioning assembly comprises a clamping plate (19) arranged at the top end of the filter cylinder (20), and the inner wall of the collection cylinder (12) is provided with a clamping groove (18) engaged with the clamping plate (19).

6. The sample pretreatment device for preparing monoclonal antibodies according to claim 5, characterized in that: The discharge assembly comprises a collecting pipe (16) arranged at the bottom end of the collecting cylinder (12); the collecting pipe (16) penetrates the rotating cylinder (4) and extends to a lower portion where a valve (17) is provided.

7. A sample pretreatment device for preparing monoclonal antibodies according to claim 6, characterized in that: The plugging assembly comprises a pressure plate (13) equidistantly arranged under the rotating plate (3), a sealing plug (14) matching the filter cartridge (20) is arranged under the pressure plate (13), and a sealing ring (15) is arranged on the peripheral side of the sealing plug (14).

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