Chip device for efficiently detecting antibiotics in water body

Abstract

The utility model provides a chip device for efficiently detecting antibiotics in a water body, which comprises a working table, a rotating bracket is arranged above the working table, one end of the working table is fixedly connected with a chip bracket, a micro-fluidic chip is fixedly connected above the chip bracket, a liquid inlet is formed in one side of the micro-fluidic chip, a sample storage box is fixedly connected above the working table, and a liquid outlet is formed in the other side of the sample storage box. A sample tube is fixedly connected in the sample storage box, one end of the sample storage box is fixedly connected with a material pushing assembly and a material discharging assembly, an electric screw rod, a sliding rod and a middle supporting plate are fixedly connected below the sample storage box, and one end of the middle supporting plate is fixedly connected with a middle pipeline; according to the utility model, the electric lead screw rotates to drive the upper needle tube to move upwards to be inserted into the sample tube, and the lower sliding tube at the lower part moves downwards to be butted with the liquid inlet on the micro-fluidic chip, so that a sample in the sample tube can be automatically introduced into the micro-fluidic chip, and then the sample tube can be pushed into the discharging assembly to recycle the waste sample tube.

Description

Technical Field

[0001] The utility model relates to the technical field of water body detection, in particular to a chip device for efficiently detecting antibiotics in water bodies. Background Art

[0002] Antibiotics are secondary metabolites or synthetic analogs produced by bacteria, molds or other microorganisms. They are widely used in the treatment of infectious diseases in humans and animals, and are also widely used in aquaculture and livestock breeding. Antibiotics cannot be fully absorbed in humans and animals, and most of them will enter sewage treatment plants or directly enter the environment in the form of prototypes or active metabolites with excrement. Therefore, it is necessary to test the antibiotic content in water bodies for early treatment. Among them, microfluidic chip technology integrates the basic operating units such as sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes into a micron-scale chip to automatically complete the entire analysis process; when testing antibiotics in water bodies, the sample and the color developer corresponding to the antibiotic to be tested can be injected into the chip, and then the fluorescence brightness can be observed after power is turned on to judge the antibiotic content.

[0003] After searching, the Chinese patent publication number CN116879281A discloses a device and method for rapid detection of antibiotics in water, including: a workbench; a support rod, the support rod is fixedly connected to the workbench, and a cross plate is fixedly connected to the support rod; a cylinder, the present invention positions the position of the positioning plate through the elastic action of the spring, and fixes the test tube through the elastic action of the positioning plate and the spring, and then drives the positioning block to move through the threaded cooperation of the bidirectional screw and the threaded sleeve, and fixes the mounting seat through the two positioning blocks.

[0004] In view of the problems existing in the above technology that test tubes need to be placed into multiple test tubes in turn during detection, which is troublesome to take and place, and the number of samples tested at one time is limited, and it is impossible to automatically inject samples into the microfluidic chip and then discharge the test tubes after the test is completed; therefore, a chip device for efficiently detecting antibiotics in water is proposed. Utility Model Content

[0005] In view of this, the embodiments of the present utility model hope to provide a chip device for efficiently detecting antibiotics in water to solve or alleviate the technical problems existing in the prior art and at least provide a beneficial option.

[0006] The technical solution of the embodiment of the utility model is achieved as follows: it includes a workbench with a rotating bracket installed on the top, one end of the workbench is fixedly connected to a chip bracket, a microfluidic chip is fixedly connected above the chip bracket, a liquid inlet is opened on one side of the microfluidic chip, a sample storage box is fixedly connected to the top of the workbench, a sample tube is fixedly connected inside the sample storage box, a pushing assembly and a discharging assembly are fixedly connected at one end of the sample storage box, an electric screw, a sliding rod and a middle support plate are fixedly connected below the sample storage box, one end of the middle support plate is fixedly connected to the middle pipeline, and the two ends are slidably connected to the lower sliding tube and the upper needle tube, the movable end of the electric screw is threadedly connected to a plurality of lifting plates, one end of the lifting plate on the upper side is fixedly connected to the upper needle tube, and one end of the lifting plate on the lower side is fixedly connected to the middle pipeline, and a sample installation port is opened above the sample storage box.

[0007] In some embodiments, a water spray plate and a recovery plate are slidably connected inside the sample storage box, one end of the water spray plate and the recovery plate are respectively fixedly connected to an inclined platform, one end of the upper and lower lifting plates are respectively fixedly connected to a driving platform, and one end of the driving platform is slidably connected to the inclined platform.

[0008] In some embodiments, a second spring is fixedly connected to the inner wall of the sample storage box, and one end of the second spring on the upper and lower sides is fixedly connected to the water spray plate and the recovery plate respectively.

[0009] In some embodiments, the pushing assembly includes a rack, a pushing plate and a driving motor, one end of the driving motor is fixedly connected to the sample storage box, one end of the rack is transmission-connected to the power output end of the driving motor, and one end of the pushing plate is fixedly connected to the rack.

[0010] In some embodiments, the discharge assembly includes a baffle, a spring and a sample recovery box, one end of the baffle is rotatably connected to the sample storage box, one end of the spring is fixedly connected to the baffle and one side of the sample storage box, and one end of the sample recovery box is fixedly connected to one side of the sample storage box.

[0011] In some embodiments, a chip box is fixedly connected above the workbench, a feed push rod is fixedly connected to one end of the chip box, and a discharge push rod and a recovery trough are fixedly connected to the other side of the workbench.

[0012] In some embodiments, a dye filling component and an energized push rod are fixedly connected to one side of the workbench, and a energized component is fixedly connected to the movable end of the energized push rod.

[0013] In some embodiments, a water supply component and a waste liquid recovery component are fixedly connected to one side of the sample storage box, one end of the water supply component is fixedly connected to the water spray plate through a pipe, and one end of the waste liquid recovery component is fixedly connected to the recovery plate through a pipe.

[0014] The embodiment of the utility model has the following advantages due to the adoption of the above technical solution:

[0015] 1. An efficient chip device for detecting antibiotics in water, which drives the upper needle tube to move upward and insert into the sample tube through the rotation of the electric screw, and moves the lower slide tube downward to dock with the liquid inlet on the microfluidic chip, so that the sample in the sample tube can be automatically introduced into the microfluidic chip, and then the sample tube can be pushed into the discharge assembly to recycle the discarded sample tube.

[0016] 2. An efficient chip device for detecting antibiotics in water, which can prevent samples from dripping to the bottom and causing contamination by closing the upper needle tube and the lower end of the lower slide tube through a water spray plate and a recovery plate, and when the two lifting plates move outward relative to each other, the driving platform can squeeze the inclined platform to squeeze the water spray plate and the recovery plate apart.

[0017] 3. An efficient chip device for detecting antibiotics in water. The water supply component can clean the upper needle tube, the middle pipe and the lower slide tube by spraying water through the nozzle under the water spray plate to prevent multiple samples from contaminating each other. The cleaned water can enter the waste liquid recovery component from the recovery plate for recovery.

[0018] The above summary is for the purpose of description only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments and features described above, further aspects, embodiments and features of the utility model will be readily apparent by reference to the accompanying drawings and the following detailed description. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or technical descriptions will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0020] Figure 1 This is the main structural diagram of the utility model;

[0021] Figure 2 This is a cross-sectional structural diagram of a sample storage box of the present utility model;

[0022] Figure 3 This is a diagram of the internal structure of the lower side of the sample storage box of the utility model;

[0023] Figure 4 This is the internal structure diagram of the chip box of the utility model;

[0024] Figure 5 This is a top view of the structure of the sample storage box of the present utility model.

[0025] Reference numerals:

[0026] 1. Workbench; 2. Rotating bracket; 3. Chip bracket; 4. Microfluidic chip; 5. Chip box; 6. Feed push rod; 7. Sample storage box; 8. Sample installation port; 9. Dye filling component; 10. Power-on component; 11. Power-on push rod; 12. Discharge push rod; 13. Recovery trough; 14. Rack; 15. Push plate; 16. Drive motor; 17. Sample tube; 18. Baffle; 19. Spring 1; 20. Water supply component; 21. Waste liquid recovery component; 22. Water spray plate; 23. Recovery plate; 24. Spring 2; 25. Lower slide tube; 26. Middle pipeline; 27. Upper needle tube; 28. Middle support plate; 29. ​​Electric screw; 30. Slide rod; 31. Lifting plate; 32. Drive table; 33. Inclined table; 34. Liquid inlet; 35. Sample recovery box. DETAILED DESCRIPTION

[0027] In the following, only some exemplary embodiments are briefly described. As those skilled in the art will appreciate, the described embodiments may be modified in various ways without departing from the spirit or scope of the present invention. Therefore, the drawings and descriptions are considered to be exemplary and non-restrictive in nature.

[0028] The embodiments of the present utility model are described in detail below with reference to the accompanying drawings.

[0029] Embodiment 1:

[0030] like Figure 1-5 As shown, an efficient chip device for detecting antibiotics in water comprises a workbench 1 with a rotating bracket 2 installed on the top, a chip bracket 3 is fixedly connected to one end of the workbench 1, a microfluidic chip 4 is fixedly connected to the top of the chip bracket 3, a liquid inlet 34 is opened on one side of the microfluidic chip 4, a sample storage box 7 is fixedly connected to the top of the workbench 1, a sample tube 17 is fixedly connected to the inside of the sample storage box 7, and the sample tube 17 is preferably a transparent plastic tube, a pusher assembly and a discharge assembly are fixedly connected to one end of the sample storage box 7, and the sample storage box 7 is fixedly connected to the sample storage box 7. An electric screw 29, a slide rod 30 and a middle support plate 28 are fixedly connected at the bottom, one end of the middle support plate 28 is fixedly connected to the middle pipe 26, and the lower slide tube 25 and the upper needle tube 27 are slidably connected at both ends. A plurality of lifting plates 31 are threadedly connected at the movable end of the electric screw 29, and the movable end of the electric screw 29 is a double-headed screw. One end of the lifting plate 31 on the upper side is fixedly connected to the upper needle tube 27, and one end of the lifting plate 31 on the lower side is fixedly connected to the middle pipe 26. A sample installation port 8 is opened above the sample storage box 7;

[0031] When testing, multiple sample tubes 17 can be placed in the upper slot of the sample storage box 7, and then the sample tube 17 can be pushed to the end of one end of the sample storage box 7 by the push assembly, and then the electric screw 29 rotates to allow the upper and lower lifting plates 31 to be relatively separated, and then the upper needle tube 27 can be driven to move upward and insert into the sample tube 17, and the lower slide tube 25 below can be moved downward to dock with the liquid inlet 34 on the microfluidic chip 4, so that the sample in the sample tube 17 can be automatically introduced into the microfluidic chip 4;

[0032] Then the pushing assembly continues to work to push the sample tube 17 into the discharging assembly to recycle the discarded sample tube 17 .

[0033] In this embodiment, the pusher assembly includes a rack 14, a push plate 15 and a drive motor 16, one end of the drive motor 16 is fixedly connected to the sample storage box 7, one end of the rack 14 is transmission-connected to the power output end of the drive motor 16, and one end of the push plate 15 is fixedly connected to the rack 14;

[0034] The discharge assembly includes a baffle 18, a spring 19 and a sample recovery box 35, one end of the baffle 18 is rotatably connected to the sample storage box 7, one end of the spring 19 is fixedly connected to the baffle 18 and one side of the sample storage box 7, and one end of the sample recovery box 35 is fixedly connected to one side of the sample storage box 7;

[0035] There is a gear at the power output end of the driving motor 16, which can drive the push plate 15 to move forward and backward in the sample storage box 7 by forward and reverse rotation. When the push plate 15 moves to one side and squeezes the sample tube 17 to the side of the baffle 18, the push plate 15 stops moving. When the sample in the sample tube 17 drips into the microfluidic chip 4, the push plate 15 continues to move forward, pushes open the baffle 18 and sends the sample tube 17 into the sample recovery box 35 for centralized recovery. The spring 19 can reset the baffle 18.

[0036] In this embodiment, a chip box 5 is fixedly connected above the workbench 1, a feed push rod 6 is fixedly connected to one end of the chip box 5, and a discharge push rod 12 and a recovery tank 13 are fixedly connected to the other side of the workbench 1;

[0037] A dye filling component 9 and an energized push rod 11 are fixedly connected to one side of the workbench 1, and an energized component 10 is fixedly connected to the movable end of the energized push rod 11;

[0038] Multiple microfluidic chips 4 are stacked in the chip box 5. When the feed push rod 6 is extended, the bottom microfluidic chip 4 can be sent to the chip holder 3 for subsequent testing. The discharge push rod 12 can send the used microfluidic chip 4 to the recovery tank 13 for recovery.

[0039] The dye filling component 9 can inject fluorescent dye into the microfluidic chip 4, and the energized push rod 11 can push the energized component 10 to contact the microfluidic chip 4 containing the fluorescent dye and the sample for detection.

[0040] In this embodiment: when testing, multiple sample tubes 17 can be placed in the upper slot of the sample storage box 7, and then the sample tube 17 can be pushed to the end of one end of the sample storage box 7 by the pushing assembly, and then the electric screw 29 rotates to allow the upper and lower lifting plates 31 to be relatively separated, and then the upper needle tube 27 can be driven to move upward and insert into the sample tube 17, and the lower slide tube 25 below can be moved downward to dock with the liquid inlet 34 on the microfluidic chip 4, so that the sample in the sample tube 17 can be automatically introduced into the microfluidic chip 4;

[0041] Then the pushing assembly continues to work to push the sample tube 17 into the discharging assembly to recycle the discarded sample tube 17;

[0042] The driving motor 16 has a gear at its power output end, which can drive the push plate 15 to move forward and backward in the sample storage box 7 by forward and reverse rotation. When the push plate 15 moves to one side and squeezes the sample tube 17 to one side of the baffle 18, the push plate 15 stops moving. When the sample in the sample tube 17 drips into the microfluidic chip 4, the push plate 15 continues to move forward, pushes the baffle 18 open, and sends the sample tube 17 into the sample recovery box 35 for centralized recovery. The spring 19 can reset the baffle 18.

[0043] Multiple microfluidic chips 4 are stacked in the chip box 5. When the feed push rod 6 is extended, the bottom microfluidic chip 4 can be sent to the chip holder 3 for subsequent testing. The discharge push rod 12 can send the used microfluidic chip 4 to the recovery tank 13 for recovery.

[0044] The dye filling component 9 can inject fluorescent dye into the microfluidic chip 4, and the energized push rod 11 can push the energized component 10 to contact the microfluidic chip 4 containing the fluorescent dye and the sample for detection.

[0045] Embodiment 2:

[0046] A chip device for efficiently detecting antibiotics in water. This embodiment makes the following improvements on the basis of embodiment 1: Figure 1-5 As shown,

[0047] In this embodiment, the sample storage box 7 is slidably connected with a water spray plate 22 and a recovery plate 23, one end of the water spray plate 22 and the recovery plate 23 are respectively fixedly connected with an inclined platform 33, and one end of the upper and lower lifting plates 31 are respectively fixedly connected with a driving platform 32, and one end of the driving platform 32 is slidably connected to the inclined platform 33;

[0048] The inner wall of the sample storage box 7 is fixedly connected to a spring 24, and one end of the upper and lower springs 24 is fixedly connected to the water spray plate 22 and the recovery plate 23 respectively;

[0049] One side of the sample storage box 7 is fixedly connected with a water supply component 20 and a waste liquid recovery component 21, one end of the water supply component 20 has a water storage box and a small water pump component, one end of the water supply component 20 is fixedly connected to the water spray plate 22 through a pipeline, and one end of the waste liquid recovery component 21 is fixedly connected to the recovery plate 23 through a pipeline, and the waste liquid recovery component 21 is a water storage box;

[0050] When the two lifting plates 31 move outwards relative to each other, the driving platform 32 can squeeze the inclined platform 33 to squeeze the water spraying plate 22 and the recovery plate 23 apart, so as to facilitate the upper needle tube 27 and the lower slide tube 25 to move outwards. When the upper needle tube 27 and the lower slide tube 25 move inwards relative to each other and shrink, the water spraying plate 22 and the recovery plate 23 can close the upper and lower ends of the upper needle tube 27 and the lower slide tube 25 under the action of the spring 24.

[0051] Then the water supply assembly 20 can clean the upper needle tube 27, the middle pipe 26 and the lower slide tube 25 by spraying water through the nozzle below the water spray plate 22 to prevent multiple samples from contaminating each other. The cleaned water can enter the waste liquid recovery component 21 from the recovery plate 23 for recovery.

[0052] The above is only a specific implementation of the utility model, but the protection scope of the utility model is not limited thereto. Any technician familiar with the technical field can easily think of various changes or substitutions within the technical scope disclosed by the utility model, which should be included in the protection scope of the utility model. Therefore, the protection scope of the utility model should be based on the protection scope of the claims.

Claims

1. An efficient chip device for detecting antibiotics in water, comprising a workbench (1) with a rotating bracket (2) mounted on top, characterized in that: The workbench (1) is fixedly connected to a chip holder (3) at one end, a microfluidic chip (4) is fixedly connected above the chip holder (3), a liquid inlet (34) is opened on one side of the microfluidic chip (4), a sample storage box (7) is fixedly connected above the workbench (1), a sample tube (17) is fixedly connected inside the sample storage box (7), a material pushing assembly and a material discharging assembly are fixedly connected to one end of the sample storage box (7), and an electric screw rod (29), a slide rod (30) are fixedly connected below the sample storage box (7). ) and a middle support plate (28), one end of the middle support plate (28) is fixedly connected to the middle pipe (26), and the two ends are slidably connected to the lower sliding pipe (25) and the upper needle tube (27), the movable end of the electric screw (29) is threadedly connected to a plurality of lifting plates (31), one end of the lifting plate (31) on the upper side is fixedly connected to the upper needle tube (27), and one end of the lifting plate (31) on the lower side is fixedly connected to the middle pipe (26), and a sample installation port (8) is opened above the sample storage box (7).

2. The chip device for efficiently detecting antibiotics in water according to claim 1, characterized in that: The sample storage box (7) is slidably connected to a water spray plate (22) and a recovery plate (23), one end of the water spray plate (22) and the recovery plate (23) are respectively fixedly connected to an inclined platform (33), one end of the upper and lower lifting plates (31) are respectively fixedly connected to a driving platform (32), and one end of the driving platform (32) is slidably connected to the inclined platform (33).

3. The chip device for efficiently detecting antibiotics in water according to claim 2 is characterized in that: The inner wall of the sample storage box (7) is fixedly connected with a second spring (24), and one end of the second spring (24) on the upper and lower sides is fixedly connected to the water spray plate (22) and the recovery plate (23) respectively.

4. The chip device for efficiently detecting antibiotics in water according to claim 1, characterized in that: The pusher assembly comprises a rack (14), a push plate (15) and a drive motor (16); one end of the drive motor (16) is fixedly connected to the sample storage box (7); one end of the rack (14) is drivingly connected to the power output end of the drive motor (16); and one end of the push plate (15) is fixedly connected to the rack (14).

5. The chip device for efficiently detecting antibiotics in water according to claim 4 is characterized in that: The discharge assembly comprises a baffle (18), a spring (19) and a sample recovery box (35); one end of the baffle (18) is rotatably connected to the sample storage box (7); one end of the spring (19) is fixedly connected to the baffle (18) and one side of the sample storage box (7); and one end of the sample recovery box (35) is fixedly connected to one side of the sample storage box (7).

6. The chip device for efficiently detecting antibiotics in water according to claim 1, characterized in that: A chip box (5) is fixedly connected above the workbench (1), a feed push rod (6) is fixedly connected to one end of the chip box (5), and a discharge push rod (12) and a recovery trough (13) are fixedly connected to the other side of the workbench (1).

7. The chip device for efficiently detecting antibiotics in water according to claim 6, characterized in that: A dye filling component (9) and an energized push rod (11) are fixedly connected to one side of the workbench (1), and a energized component (10) is fixedly connected to the movable end of the energized push rod (11).

8. The chip device for efficiently detecting antibiotics in water according to claim 3 is characterized in that: A water supply component (20) and a waste liquid recovery component (21) are fixedly connected to one side of the sample storage box (7); one end of the water supply component (20) is fixedly connected to a water spray plate (22) via a pipeline, and one end of the waste liquid recovery component (21) is fixedly connected to a recovery plate (23) via a pipeline.

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