Electrophoresis gel preparation demolding device

Abstract

The utility model discloses an electrophoresis gel preparation demolding device which comprises a base, a mold shell, a sieve plate and a pushing mechanism, and a supporting plate is arranged on the base; the mold shell is fixed to the supporting plate, a mold cavity is formed in the middle of the mold shell, and a mold plate is slidably connected into the mold cavity. A concave step surface is arranged on the side edge of the upper surface of the template, and sieve pores are uniformly distributed in the step surface; the pushing mechanism comprises a pushing motor, a guide column and a pushing plate, the pushing motor and the guide column are fixed to the base, and a lead screw is fixed to the output end of the pushing motor; the push plate is fixed at the lower end of the template, and a threaded hole and a guide hole are formed in the push plate; the push plate is slidably connected to the guide columns through the guide holes. The sieve plate is fixed to the base through the guide columns, sieve teeth matched with the sieve holes are fixed to the upper surface of the sieve plate, and the sieve teeth are slidably connected into the sieve holes. The utility model provides an electrophoresis gel preparation demoulding device, which can demould an electrophoresis gel mould body prepared in a mould and reduce the condition that the gel is broken due to manual stripping.

Description

Technical Field

[0001] The utility model relates to the technical field of DNA electrophoresis, in particular to a demoulding device for preparing electrophoresis gel. Background Art

[0002] Gel electrophoresis technology is currently a widely used method in the process of DNA separation. Agarose gel and polyacrylamide gel are currently more widely used. Agarose gel is deeply loved by laboratories because of its low cost and intuitive bands.

[0003] Although the Young's modulus of agarose gel is similar to that of human air organs or other biological tissues, the tensile and compressive strengths are very different. The tensile and compressive strengths of agarose gel are much lower than those of biological tissues. This means that in order to ensure that the experimental effect is close to the biological tissue to be simulated, the prepared agarose gel will be very fragile. In addition, since it is essentially sugar, there is still stickiness on the surface. In experiments, the prepared agarose gel is generally peeled off from the mold manually. Because the force cannot be accurately controlled, the experimenter is often required to concentrate highly and perform the demolding operation very carefully. However, even so, the gel model may collapse, affecting the progress of the experiment. Summary of the invention

[0004] In order to overcome the above-mentioned deficiencies of the prior art, the utility model provides an electrophoresis gel preparation demoulding device, which can demould the electrophoresis gel mold prepared in the mold and reduce the gel collapse caused by manual peeling.

[0005] The utility model is realized by the following technical solutions:

[0006] The demolding device for preparing electrophoresis gel comprises a base, a mold shell, a sieve plate and a pushing mechanism, wherein the base is provided with a support plate; the mold shell is fixed to the support plate, and a through membrane cavity is provided in the middle of the mold shell, and a template is slidably connected to the membrane cavity; a concave step surface is provided on the side of the upper surface of the template, and a plurality of sieve holes are evenly distributed on the step surface along the length direction; the pushing mechanism comprises a pushing motor, a guide column and a pushing plate, and the pushing motor and the guide column are fixed on the base, and a screw rod is fixed on the output end of the pushing motor; the pushing plate is fixed to the lower end of the template, and a threaded hole and a guide hole are provided on the pushing plate; the threaded hole is adapted to the screw rod; the pushing plate is slidably connected to the guide column through the guide hole; the sieve plate is fixed to the base through the guide column, and a plurality of sieve teeth adapted to the sieve holes are fixed on the upper surface of the sieve plate, and the sieve teeth are slidably connected to the sieve holes; in the technical scheme, when the device is in the initial state, the template is located at the bottom of the mold shell, and the sieve teeth penetrate The sieve holes are placed in the mold shell. After the electrophoresis gel solution is poured, it can be left to solidify to form a gel mold body, and a number of sample addition holes are formed on the mold body to facilitate multiple groups of experiments. The sieve teeth are located on the concave step surface, so that the prepared mold body forms a convex block at the sample addition hole. When the electrophoresis liquid is added to cover the mold body, the convex block can be above the liquid surface and not immersed in the electrophoresis liquid to prevent the sample in the sample addition hole from usurping its position. During the demoulding process, the pushing mechanism pushes the template to move upward. Along with the movement of the template, the sieve teeth can be pulled out of the mold body, so that the sieve teeth can be pulled out of the mold body; the pushing mechanism adopts a screw drive mode, which can control the movement feed amount of the template, gently move the mold body out of the membrane cavity, and avoid the gel from collapsing. The push plate is slidably connected to the guide column, which can assist in positioning the movement of the template. When the screw rotates, it takes over part of the transmission torque, reduces the friction load between the template and the mold shell, and makes the template move more smoothly.

[0007] Preferably, the push plate has a U-shaped structure, including a base plate and side plates welded to both ends of the base plate; the threaded hole is fixed in the middle of the base plate; the template is detachably connected to the two side plates; in this technical solution, the push plate is a welded structure, which can be connected to the template by bolt fastening, which can facilitate the subsequent replacement of parts.

[0008] Preferably, there are two guide posts, which are symmetrically arranged on both sides of the push motor; in this technical solution, the guide posts are symmetrically arranged on both sides of the push motor, which can enhance the uniformity of force on the screen plate.

[0009] Preferably, a first sealing groove is provided on the side wall of the template, and a first sealing ring is provided in the first sealing groove; in this technical solution, the first sealing ring can enhance the sealing between the template and the chamber to prevent the electrophoresis gel from leaking from the gap therebetween.

[0010] Preferably, a second sealing groove is provided on the inner wall of the sieve hole, and a second sealing ring is provided in the second sealing groove; in this technical solution, the first sealing ring can enhance the sealing between the sieve hole and the sieve teeth to prevent the electrophoresis gel from leaking from the gap therebetween.

[0011] Preferably, an annular shell is provided between the support plate and the base, and the push motor is placed in the inner cavity of the annular shell; in this technical solution, the annular shell can protect the push motor, so that the screw rod and the guide column are in a clean environment, preventing dust from adhering to the surface and affecting the operation of the device.

[0012] Preferably, a cover body is hinged on the support plate, a closed space is formed between the cover body and the support plate, and the mold shell is placed in the closed space; in this technical solution, the cover body can protect the mold shell to prevent other debris from entering it during the preparation process and damaging the mold body.

[0013] Further preferably, a vacuum pump is provided on the base, and a suction port is provided on the support plate, and the suction port is connected to the vacuum pump through a pipe; in the present technical solution, since the electrophoresis gel often needs to undergo a high-temperature process during the preparation process, and this process will cause the gas to dissolve into the gel solution, bubbles often appear in the finally formed gel, thus affecting the experimental results and observations. The vacuum pump forms a vacuum environment in the mold shell to degas the electrophoresis gel, which is not easy to generate bubbles.

[0014] Further preferably, a third sealing groove is provided at the contact edge between the cover body and the support plate, and a third sealing ring is provided in the third sealing groove; in this technical solution, the third sealing groove can enhance the sealing performance between the cover body and the support plate.

[0015] The beneficial effects of the utility model are as follows: 1. The electrophoresis gel mold in the device includes a mold shell and a template, the template is slidably connected in the membrane cavity of the membrane shell, and a pushing mechanism is provided at the bottom of the template, the pushing mechanism adopts a screw drive to move the template, and can gently remove the mold body from the membrane cavity, reducing the gel collapse caused by manual peeling; 2. The template in the device is provided with a concave step surface, and the sieve hole is located on the step surface, which can make the prepared mold body loading hole protrude from the electrophoresis liquid surface and not be immersed in the electrophoresis liquid, thereby avoiding the sample in the loading hole from usurping the position in the electrophoresis liquid; 3. The device is provided with a cover body, the cover body can seal the membrane shell in a closed space for protection, and the closed space can be vented by a vacuum pump to degas the molded agarose gel and not easily generate bubbles. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a schematic structural diagram of a demoulding device for preparing an electrophoresis gel in a film-making state as described in Example 1;

[0017] Figure 2 This is a schematic structural diagram of a demoulding device for preparing an electrophoresis gel in a demoulding state as described in Example 1;

[0018] Figure 3 It is a schematic diagram of the partial structure of demoulding of a demoulding device for preparing an electrophoresis gel described in Example 1;

[0019] Figure 4 It is a structural schematic diagram of the mold shell described in Example 1;

[0020] Figure 5 It is a structural schematic diagram of the pushing mechanism described in Example 1;

[0021] Figure 6 It is a schematic diagram of the structure of the phantom described in Example 1;

[0022] In the figure: 1. base; 11. annular shell; 12. buckle; 13. vacuum pump; 2. support plate; 21. suction port; 22. air pressure sensor; 3. mold shell; 31. membrane cavity; 32. positioning flange; 4. template; 41. first sealing ring; 42. step surface; 421. sieve hole; 422. second sealing ring; 5. sieve plate; 51. sieve teeth; 6. guide column; 7. push plate; 71. bottom plate; 72. side plate; 711. threaded hole; 712. guide hole; 8. push motor; 81. screw rod; 9. cover body; 91. third sealing ring; 10. mold body; 101. sample adding hole. DETAILED DESCRIPTION

[0023] The following will be combined with the accompanying drawings to clearly and completely describe the technical solutions of the various embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0024] Embodiment 1

[0025] like Figures 1 to 6As shown, a demoulding device for preparing electrophoresis gel includes a base 1, a mold shell 3, a sieve plate 5 and a pushing mechanism, wherein the base 1 is provided with a support plate 2; an opening is provided in the middle of the support plate 2, and the mold shell 3 is fixed to the opening of the support plate 2 by a positioning flange 32 provided on the side, and the positioning flange 32 can be fixed to the support plate 2 by bolt connection. In order to enhance the tight connection between the positioning flange 32 and the support plate 2, a rubber pad is provided between the positioning flange 32 and the support plate 2; a rectangular membrane cavity 31 is provided in the middle of the mold shell 3, and a template 4 is slidably connected to the membrane cavity 31; a concave step surface 42 is provided on the side of the upper surface of the template 4, and the upper edge of the step surface 42 is provided with a long In order to enhance the air tightness between the mold shell 3 and the template 4, the four corners of the template 4 are arc-shaped, which is convenient for setting the first sealing groove on the side wall, and the first sealing groove is provided with a first sealing ring 41; the pushing mechanism includes a pushing motor 8, a guide column 6 and a pushing plate 7, the pushing motor and the guide column are fixed on the base, and a screw 81 is fixed to the output end of the pushing motor. Specifically, the pushing motor 8 is a servo motor, which is electrically connected to the control unit of the device and can accurately control the rotation speed and displacement; the guide columns are two symmetrically arranged on both sides of the pushing motor; the pushing plate 7 is a U-shaped structure, including a bottom plate 71 and side plates 72 welded at both ends of the bottom plate, and the side plates 72 are welded at both ends of the bottom plate. The plate 72 is fixed to the lower end of the template 4 by bolt connection, and a threaded hole 711 and a guide hole 712 are provided on the bottom plate 71; the threaded hole 711 is adapted to the screw rod 81; the push plate 7 is slidably connected to the guide column 6 through the guide hole 712; the sieve plate 5 is fixed to the top of the guide column 6, and a plurality of sieve teeth 51 adapted to the sieve holes are fixed on the upper surface of the sieve plate 5, and the sieve teeth 51 are slidably connected in the sieve holes 421. In order to enhance the air tightness of the connection between the sieve teeth 51 and the sieve holes 421, a second sealing groove is provided on the inner wall of the sieve holes 421, and a second sealing ring 422 is provided in the second sealing groove; in the initial state of the device, the template 4 is located at the bottom of the rectangular membrane cavity 31 of the mold shell, The sieve teeth 51 pass through the sieve holes 421 and are placed in the rectangular membrane cavity 31 of the mold shell. After the electrophoresis gel solution is injected, it is left to solidify and a gel mold body 10 can be formed, and a plurality of sample loading holes 101 are formed on the mold body 10 to facilitate multiple groups of experiments. The sieve teeth 51 are located on a concave step surface, so that the prepared mold body forms a convex block at the sample loading hole. When the electrophoresis liquid is added to cover the mold body, the convex block can be above the liquid surface and not immersed in the electrophoresis liquid to prevent the sample in the sample loading hole 101 from usurping its position. When demolding, the push motor 8 rotates, and the screw 81 pushes the template 4 to move upward. Along with the movement of the template 4, the sieve teeth 51 can be pulled out of the mold body, so that the sieve teeth can be pulled out of the mold body.The pushing mechanism adopts the screw drive mode, which can control the movement and feeding amount of the template, gently move the mold body out of the membrane cavity, and avoid the gel collapse. The push plate is slidably connected to the guide column 6, which can assist in positioning the movement of the template. When the screw 81 rotates, it takes over part of the transmission torque, reduces the friction load between the template and the mold shell, and makes the template move more smoothly.

[0026] Preferably, an annular shell 11 is provided between the support plate 2 and the base 1, and the pushing mechanism is placed in the inner cavity of the annular shell 11. The annular shell 11 can protect the pushing mechanism, so that the screw rod and the guide column are in a clean environment, preventing dust from adhering to the surface and affecting the operation of the device; a cover body 9 is hinged on the support plate 2, and a closed space is formed between the cover body 9 and the support plate 2. The mold shell 3 is placed in the closed space to prevent other debris from entering the closed space during the preparation process and damaging the mold body; further, a vacuum pump 13 is provided on the base 1, and the A suction port 21 and an air pressure sensor 22 are provided on the support plate 2. The suction port 21 is connected to a vacuum pump through a pipeline. The vacuum pump and the air pressure sensor 22 are electrically connected to the control unit of the device, so that the mold 10 can be solidified in an environment of a preset vacuum degree, making it difficult to generate bubbles and convenient for observing the mold. In order to enhance the sealing performance between the cover body 9 and the support plate 2, a third sealing groove is provided on the contact edge of the cover body 9 and the support plate 2, and a third sealing ring 91 is provided in the third sealing groove, and a buckle 12 is provided on the annular shell 11 to fix the cover body 9.

[0027] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the utility model, rather than to limit it. Although the utility model has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solution recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solution from the technical solution of the embodiments of the utility model.

Claims

1. A demoulding device for preparing electrophoresis gel, characterized in that: It includes a base, a mold shell, a sieve plate and a pushing mechanism, wherein the base is provided with a support plate; the mold shell is fixed on the support plate, and a through membrane cavity is provided in the middle of the mold shell, and a template is slidably connected in the membrane cavity; a concave step surface is provided on the side of the upper surface of the template, and a plurality of sieve holes are evenly distributed on the step surface along the length direction; the pushing mechanism includes a pushing motor, a guide column and a pushing plate, the pushing motor and the guide column are fixed on the base, and a screw rod is fixed on the output end of the pushing motor; the pushing plate is fixed to the lower end of the template, and a threaded hole and a guide hole are provided on the pushing plate; the threaded hole is matched with the screw rod; the pushing plate is slidably connected to the guide column through the guide hole; the sieve plate is fixed to the base through the guide column, and a plurality of sieve teeth matched with the sieve holes are fixed on the upper surface of the sieve plate, and the sieve teeth are slidably connected in the sieve holes.

2. The electrophoresis gel preparation and demoulding device according to claim 1, characterized in that: The push plate is in a U-shaped structure, including a bottom plate and side plates welded to both ends of the bottom plate; the threaded hole is fixed in the middle of the bottom plate; the template is detachably connected to the two side plates.

3. The electrophoresis gel preparation and demoulding device according to claim 1, characterized in that: There are two guide pillars, which are symmetrically arranged on both sides of the push motor.

4. The electrophoresis gel preparation and demoulding device according to claim 1, characterized in that: A first sealing groove is arranged on the side wall of the template, and a first sealing ring is arranged in the first sealing groove.

5. The electrophoresis gel preparation and demoulding device according to claim 1, characterized in that: A second sealing groove is provided on the inner wall of the sieve hole, and a second sealing ring is provided in the second sealing groove.

6. The electrophoresis gel preparation and demoulding device according to claim 1, characterized in that: An annular shell is arranged between the support plate and the base, and the pushing motor is arranged in the inner cavity of the annular shell.

7. The electrophoresis gel preparation and demoulding device according to claim 1, characterized in that: A cover body is hinged on the support plate, a closed space is formed between the cover body and the support plate, and the mold shell is placed in the closed space.

8. The electrophoresis gel preparation and demoulding device according to claim 7, characterized in that: The base is provided with a vacuum pump, the support plate is provided with a suction port, and the suction port is connected to the vacuum pump through a pipeline.

9. The electrophoresis gel preparation and demoulding device according to claim 8, characterized in that: A third sealing groove is provided at the contact edge between the cover body and the support plate, and a third sealing ring is provided in the third sealing groove.

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