Strain inoculation device for fermentation

Abstract

The utility model discloses a strain inoculation device for fermentation, which relates to the technical field of biological fermentation and comprises a fermentation tank. The inoculation bottle is fixed on the fermentation tank and is communicated with the interior of the fermentation tank; the cover body is detachably connected with the inoculation opening of the inoculation bottle, the cover body is provided with a first exhaust opening, and the first exhaust opening is configured to be capable of controlling communication between the interior of the inoculation bottle and the outside; and the sterilization and disinfection assembly is erected outside the inoculation opening. The strain inoculation device for fermentation is reasonable in design, parts of the inoculation part of the device can be effectively disinfected, it is guaranteed that no infectious microbe is mixed into the inoculation bottle before inoculation, and the situation that the infectious microbe is mixed into the inoculation bottle before inoculation and after inoculation can be greatly reduced in cooperation with use of the sterilization and disinfection assembly.

Description

Technical Field

[0001] The utility model relates to the technical field of biological fermentation, in particular to a bacterial strain inoculation device for fermentation. Background Art

[0002] In the process of fermentation and amplification of microbial strains, the seed liquid in the shake flask needs to be introduced into the inoculation bottle, so that the inoculation bottle is exposed to the natural environment. The inoculation process faces the situation that microorganisms in the environment contaminate the inoculation bottle and the fermentation tank; the commonly used methods of inoculation include differential pressure inoculation and flame inoculation: these two inoculation methods usually adopt corresponding sterilization methods, but for the differential pressure method sterilization process: put a gasket before sterilization, open the inoculation port cover during inoculation, first pour a certain amount of alcohol for disinfection, wait for a while, insert the needle of the seed liquid bottle into the gasket of the inoculation port, use the pressure difference between the pressure in the tank and the pressure in the seed liquid bottle to introduce the seed liquid into the tank, and tighten the lid; and for the flame inoculation method sterilization: disinfect the inoculation port with an alcohol fire ring, then open the inoculation port cover, quickly pour the inoculation liquid into the tank, and then tighten the lid. Although the flame inoculation method has a relatively simple process, the existing inoculation device disinfection process is cumbersome, and the disinfection of the inoculation bottle is not thorough. The bottle cap must be opened each time the inoculation bottle is disinfected, which can easily lead to external bacteria being brought into the inoculation bottle after the disinfection is completed, and the flame sterilization ring usually needs to be held manually, and cannot be fixed at multiple angles and dimensions. For the inoculation device in which the shaking bottle is connected to the inoculation bottle through a catheter, higher requirements are placed on the sterilization method. Although flame sterilization is more commonly used in the sterilization process, the flame can easily cause the catheter to burn or dissolve, causing damage to the catheter. Therefore, the flame sterilization method is not suitable for catheter-accessible inoculation devices. Utility Model Content

[0003] The utility model aims to solve at least one of the above-mentioned technical problems in the prior art to a certain extent. To this end, the embodiment of the utility model provides a fermentation strain inoculation device, which introduces seed liquid into the inoculation bottle through the catheter-type access of the shaking bottle and the inoculation bottle, and only the connected catheter and its surroundings are sterilized with high-temperature and high-pressure steam to achieve effective sterilization in a local range without damaging the catheter; or when the seed liquid is quickly poured into the inoculation bottle by opening the inoculation bottle cap, the flame ring with a bendable auxiliary rod can fix the inoculation bottle and the surrounding of the bottle mouth at multiple angles and directions and sterilize the inoculation bottle. The utility model has a reasonable design and can effectively sterilize and disinfect the components of the inoculation bottle part of the device, and can also avoid the mixing of miscellaneous bacteria before and during inoculation.

[0004] According to the embodiment of the utility model, the fermentation strain inoculation device includes a fermentation tank; an inoculation bottle, which is fixed to the fermentation tank and connected to the interior of the fermentation tank; a cover body, which is detachably connected to the inoculation port of the inoculation bottle, and the cover body has a first exhaust port, and the first exhaust port is configured to control the connection between the interior of the inoculation bottle and the outside world; and a sterilization and disinfection component, which is mounted outside the inoculation port.

[0005] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the cover body is threadedly connected to the inoculation port, the first exhaust port is arranged on the side of the cover body, the inoculation port is provided with an exhaust structure, and the exhaust structure includes a second exhaust port, wherein the first exhaust port can be rotated to face the second exhaust port.

[0006] According to the fermentation strain inoculation device of the embodiment of the utility model, the fermentation tank is provided with a connecting pipe for communicating with the inoculation bottle, and a diaphragm valve is provided on the connecting pipe.

[0007] According to the fermentation strain inoculation device of the embodiment of the utility model, the sterilization and disinfection component includes a disinfection ring and an auxiliary rod, the auxiliary rod can be bent, the auxiliary rod is fixedly connected to the disinfection ring, the disinfection ring is used to release flame or high-temperature airflow, and a fixed rod is provided on the fermentation tank, the auxiliary rod is inserted into the fixed rod or the fermentation tank, so that the disinfection ring is erected outside the inoculation port.

[0008] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the disinfection ring is provided with a groove, a wick is arranged in the groove, and the wick is used to adsorb alcohol.

[0009] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the sterilization and disinfection component also includes a fire extinguishing cover, and the fire extinguishing cover is used to cover the disinfection ring.

[0010] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the interior of the disinfection ring is hollow, the inner side and the upper side of the disinfection ring are arranged with release holes in an array, and the disinfection ring is also provided with a gas joint for connecting to an external gas source.

[0011] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the auxiliary rod includes a first connecting section, a second connecting section and a third connecting section, one end of the first connecting section is fixedly connected to the disinfection ring, the other end of the first connecting section is connected to one end of the second connecting section through a first limiting structure, and the other end of the second connecting section is connected to the third connecting section through a second limiting structure, wherein, under the action of the first limiting structure, the second connecting section has a first state and a second state, in the first state, the first connecting section and the second connecting section are collinear, in the second state, the first connecting section and the second connecting section are bent at a certain angle, and in some cases, the first connecting section is perpendicular to the second connecting section; under the action of the second limiting structure, the third connecting section has a third state and a fourth state, in the third state, the second connecting section and the third connecting section are collinear, in the fourth state, the second connecting section and the third connecting section are bent at a certain angle, and in some cases, the second connecting section is perpendicular to the third connecting section.

[0012] According to the fermentation bacteria inoculation device of the embodiment of the utility model, in the second state, the first connecting section is perpendicular to the second connecting section; in the fourth state, the second connecting section is perpendicular to the third connecting section.

[0013] According to the fermentation strain inoculation device of the embodiment of the utility model, the first limiting structure includes a first groove, a first connecting part and a first adjusting knob, the first groove is arranged in the first connecting section, the first connecting part is fixed to the second connecting section, the first connecting part is hinged to the first groove, the first adjusting knob is arranged in the first connecting section and can enter the first groove to limit the movement of the first connecting part, and the second connecting section can be switched between the first state and the second state through the first adjusting knob.

[0014] According to the fermentation strain inoculation device of the embodiment of the utility model, the first adjusting knob is threadedly connected to the first connecting section, and the first connecting portion is provided with a first limiting hole. In the first state, the first adjusting knob is located in the first limiting hole. In the second state, one side of the first connecting portion abuts against the bottom of the first groove, and the end of the first adjusting knob abuts against the other side of the first connecting portion.

[0015] According to the fermentation strain inoculation device of the embodiment of the utility model, the second limiting structure includes a second groove, a second connecting part and a second adjusting knob, the second groove is arranged in the second connecting section, the second connecting part is fixed to the third connecting section, the second connecting part is hinged to the second groove, the second adjusting knob is arranged in the second connecting section and can enter the second groove to limit the movement of the second connecting part, and the third connecting section can be switched between the second state and the third state through the second adjusting knob.

[0016] According to the fermentation strain inoculation device of the embodiment of the utility model, the second adjusting knob is threadedly connected to the second connecting section, and the second connecting portion is provided with a second limiting hole. In the third state, the end of the second adjusting knob is located in the second limiting hole. In the fourth state, one side of the second connecting portion abuts against the bottom of the second groove, and the end of the second adjusting knob abuts against the other side of the second connecting portion.

[0017] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the exhaust structure includes a first shell and a blocking piece, one end of the first shell is open and connected to the second exhaust port, the other end of the first shell has a third exhaust port, the blocking piece is movably arranged in the first shell, and the blocking piece blocks the third exhaust port under the action of the cover body, wherein when the second exhaust port is aligned with the first exhaust port, the blocking piece can extend from the second exhaust port to the outside of the inoculation port.

[0018] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the first shell is provided with a limit rod, and the limit rod is used to limit the movable range of the blocking member.

[0019] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the limiting rod is inserted into the blocking member along the radial direction of the first shell, and the first shell is provided with a groove for the end of the limiting rod to move.

[0020] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the blocking member is one of silica gel, rubber, and eutectic, and the end of the blocking member has a spherical surface or a conical surface.

[0021] According to the fermentation bacteria inoculation device of the embodiment of the utility model, the cover body is provided with a handle, and the handle is hingedly or detachably connected to the cover body.

[0022] Based on the above technical scheme, the embodiment of the utility model has at least the following beneficial effects: in the above technical scheme, when the cover body and the inoculation bottle are initially steam sterilized, the inoculation port of the inoculation bottle is blocked by the cover body, and the first exhaust port of the cover body is adjusted to the inside of the inoculation bottle and connected to the outside world. At this time, the high-temperature steam of the fermentation tank is released into the inoculation bottle, and the high-temperature steam sterilizes the inside of the inoculation bottle and releases it to the outside world through the first exhaust port. This process also sterilizes the cover body at high temperature. After the initial sterilization is completed, the steam supply is disconnected, and the first exhaust port is adjusted to the inside of the inoculation bottle and not connected to the outside world, to ensure that miscellaneous bacteria cannot enter the inoculation bottle, and then the sterilization and sterilization component is activated. The seed liquid is introduced into the inoculation bottle through the catheter-type access of the shaking bottle and the inoculation bottle, and the high-temperature and high-pressure steam is used to sterilize the bacteria only for the connected catheter and its surroundings to achieve effective sterilization within a local range without damaging the catheter; and when the seed liquid is quickly poured into the inoculation bottle by opening the cap of the inoculation bottle, the flame ring with a bendable auxiliary rod can fix the inoculation bottle and the surrounding of the bottle mouth at multiple angles and directions and sterilize, that is, the sterilization and disinfection component continuously generates high-temperature open flames or high-temperature steam to kill the bacteria outside the inoculation port and outside the cover of the two different inoculation devices. During the opening of the cover body and the inoculation process, the sterilization and disinfection component always works until the inoculation is completed and the cover body seals the inoculation port. The present application solves the problem of sucking in external air and bringing in bacteria when the temperature in the inoculation bottle drops relatively quickly after the disinfection is completed when steam disinfection is used in the past. The additional first exhaust port is conducive to ensuring that there is no bacteria mixed in the inoculation bottle before inoculation. With the use of the sterilization and disinfection component, the situation of bacteria mixing before and after inoculation can be greatly reduced. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The utility model is further described below in conjunction with the accompanying drawings and embodiments;

[0024] Figure 1 This is a schematic diagram of the structure of the embodiment of the utility model Figure 1 ;

[0025] Figure 2 This is a schematic diagram of the structure of the embodiment of the utility model Figure 2 ;

[0026] Figure 3 This is a schematic diagram of the connection between the inoculation bottle and the cover body in an embodiment of the utility model;

[0027] Figure 4 yes Figure 3 The cross-sectional view along the AA direction;

[0028] Figure 5 yes Figure 4 The enlarged schematic diagram of point B in the middle;

[0029] Figure 6This is a schematic diagram of the structure of the sterilization and disinfection component in the embodiment of the utility model. Figure 1 ;

[0030] Figure 7 This is a schematic diagram of the structure of the sterilization and disinfection component in the embodiment of the utility model. Figure 2 ;

[0031] Figure 8 This is a schematic diagram of the structure of the sterilization and disinfection component in the embodiment of the utility model. Figure 3 ;

[0032] Fig. 9 It is a working schematic diagram of the connection between the shake flask and the inoculation bottle.

[0033] Reference numerals:

[0034] Fermentation tank 100, fixing rod 110, connecting pipe 120, diaphragm valve 121, seventh fixing member 130; inoculation bottle 200, inoculation port 201, second exhaust port 202, cover 210, handle 211, first exhaust port 212, sealing pad 220, exhaust structure 230, first shell 231, third exhaust port 2311, groove 2312, blocking member 232, limiting rod 233; first fixing member 310, second fixing member 320, third fixing member 330, fourth fixing member 340, fifth fixing member 350, sixth fixing member 360; Sterilization and disinfection component 400, disinfection ring 410, release hole 411, gas joint 412, combustion core 420, auxiliary rod 430, first connecting section 431, first groove 4311, first hinge 4312, first adjusting knob 4313, second connecting section 432, first connecting part 4321, first limiting hole 4321a, second groove 4322, second hinge 4323, second adjusting knob 4324, third connecting section 433, second connecting part 4331, fire extinguishing cover 440, shaking bottle 500, and delivery tube 510. DETAILED DESCRIPTION

[0035] This section will describe in detail the specific embodiments of the utility model. The preferred embodiments of the utility model are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the utility model, but it cannot be understood as a limitation on the protection scope of the utility model.

[0036] In the description of the present invention, it should be understood that descriptions involving orientation, such as up, down, front, back, left, right, etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0037] In the description of the present utility model, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood to exclude the number itself, and "above", "below", "within" etc. are understood to include the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0038] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0039] Reference Figure 1 and Figure 2 , the fermentation strain inoculation device of the present application comprises a fermentation tank 100, an inoculation bottle 200, a cover 210 and a sterilization and disinfection component 400, the fermentation tank 100 is mainly used for biological fermentation, the inoculation bottle 200 is fixed to the fermentation tank 100 and is connected to the interior of the fermentation tank 100, specifically, the fermentation tank 100 is provided with a connecting pipe 120 for connecting the inoculation bottle 200, the end of the connecting pipe 120 is connected to the inoculation bottle 200 through a flange structure, wherein the connecting pipe 120 is also provided with a diaphragm valve 121, the diaphragm valve 121 is used to control the connection between the inoculation bottle 200 and the fermentation tank 100, during inoculation, the connected strain enters the inoculation bottle 200, and then enters the fermentation tank 100 through the connecting pipe 120; and the cover 210 is detachably connected to the inoculation port 201 of the inoculation bottle 200, in the present application, the cover 210 has a first exhaust port 212, and the first exhaust port 212 is configured to control the interior of the inoculation bottle 200 It is connected to the outside world to reduce the probability of foreign bacteria entering the inoculation bottle 200 after the steam sterilization is completed. In addition, the sterilization and disinfection component is then activated, and the seed liquid is introduced into the inoculation bottle through the catheter-type access of the shaking bottle and the inoculation bottle, and only the connected catheter and its surroundings are subjected to high-temperature and high-pressure steam to disinfect foreign bacteria in a local range, and effective sterilization is performed without damaging the catheter; and when the bottle cap of the inoculation bottle is opened to quickly pour the seed liquid into the inoculation bottle, the flame ring with a bendable auxiliary rod can fix the inoculation bottle and the surrounding area of ​​the bottle mouth at multiple angles and directions and sterilize the bottle, that is, the sterilization and disinfection component continuously generates high-temperature open flame or high-temperature steam to kill foreign bacteria outside the inoculation port and outside the cover of the two different types of inoculation devices, and the sterilization and disinfection component 400 is set outside the inoculation port 201, and the sterilization and disinfection component 400 is used to release flame or high-temperature steam to disinfect foreign bacteria at the inoculation port 201, ensuring that no foreign bacteria enter the inoculation process.

[0040] Specifically, when the cover body 210 and the inoculation bottle 200 are initially steam sterilized, the inoculation port 201 of the inoculation bottle 200 is blocked by the cover body 210, and the first exhaust port 212 of the cover body 210 is adjusted to the inside of the inoculation bottle 200 to be connected to the outside. At this time, the high-temperature steam of the fermentation tank 100 is released into the inoculation bottle 200, and the high-temperature steam sterilizes the inside of the inoculation bottle 200 and is released to the outside through the first exhaust port 212. This process also sterilizes the cover body 210. After the initial sterilization is completed, the first exhaust port 212 is adjusted to the inside of the inoculation bottle 200 and is not connected to the outside to ensure that miscellaneous bacteria cannot enter the inoculation bottle 200, and then the sterilization and sterilization component is activated, and the bottle is shaken and inoculated. The catheter-type access of the bottle introduces the seed liquid into the inoculation bottle, and only the connected catheter and its surrounding area are subjected to high-temperature and high-pressure steam to disinfect bacteria in a local area, thereby achieving effective sterilization without damaging the catheter; and when the bottle cap of the inoculation bottle is opened to quickly pour the seed liquid into the inoculation bottle, the flame ring with a bendable auxiliary rod can fix the inoculation bottle and the surrounding area of ​​the bottle mouth at multiple angles and directions to sterilize, that is, the sterilization and disinfection component 400 is activated, and the sterilization and disinfection component 400 continuously generates high-temperature open flames or high-temperature steam to kill bacteria outside the inoculation port 201 and outside the cover body 210. When the cover body 210 is opened and the inoculation process, the sterilization and disinfection component 400 always works until the inoculation is completed and the cover body 210 seals the inoculation port 201. The existing inoculation bottle 200 needs to open the cover 210 for disinfection. Even if the cover 210 is quickly closed after disinfection, it is easy for external bacteria to be brought into the inoculation bottle 200 after disinfection. The present application can perform steam disinfection without opening the cover 210, eliminating the situation where opening and closing the cover 210 brings in bacteria. The newly added first exhaust port 212 is helpful to ensure that no bacteria are mixed in the inoculation bottle 200 before inoculation.

[0041] like Figure 1 and Figure 2As shown, the first exhaust port 212 is arranged on the side of the cover body 210, and the cover body 210 is threadedly connected to the inoculation port 201. The way in which the cover body 210 is threadedly connected to the inoculation port 201 is convenient for disassembly and assembly on the one hand, and the thread can also play a good sealing effect on the other hand, effectively reducing the probability of miscellaneous bacteria mixing into the inoculation bottle 200. Further, the inoculation port 201 is provided with an exhaust structure 230, and the exhaust structure 230 includes a second exhaust port 202, wherein the first exhaust port 212 can be rotated to face the second exhaust port 202. When the cover body 210 is threadedly connected to the inoculation port 201 and the first exhaust port 2 When 12 is not aligned with the second exhaust port 202, the inoculation bottle 200 is effectively sealed. When the first exhaust port 212 is rotated to face the second exhaust port 202, the interior of the inoculation bottle 200 can be connected with the outside world. In this way, the connection between the inside of the inoculation bottle 200 and the outside world can be controlled by screwing the cover body 210 without opening the cover body 210, which is convenient for steam sterilization. In addition, after the sterilization is completed, the knob cover body 210 can be used to achieve rapid sealing to avoid the influx of miscellaneous bacteria after sterilization, and it can also ensure that the inoculation bottle 200 is kept warm and pressurized before inoculation, thereby improving the yield and success rate of inoculation.

[0042] like Figures 3 to 5 In the illustrated embodiment, the exhaust structure 230 includes a first shell 231 and a blocking member 232. One end of the first shell 231 is open and docked with the second exhaust port 202 provided on the inoculation port 201. The other end of the first shell 231 has a third exhaust port 2311. The blocking member 232 is movably provided in the first shell 231. The blocking member 232 blocks the third exhaust port 2311 under the action of the cover 210. When the second exhaust port 202 is aligned with the first exhaust port 212, the blocking member 232 can extend from the second exhaust port 202 to the outside of the inoculation port 201. In a normal state, as shown in FIG. Figure 5As shown, part of the blocking member 232 extends out of the first shell 231, so that when the cover body 210 is screwed, the blocking member 232 can be squeezed to move toward the third exhaust port 2311 and achieve sealing through deformation, thereby ensuring that the air pressure in the inoculation bottle 200 will not leak from the third exhaust port 2311; when air disinfection is performed, the cover body 210 is screwed to rotate the first exhaust port 212 to face the exhaust structure 230, and the blocking member 232 returns to its original state and releases the blockage of the third exhaust port 2311 under the action of the airflow, so that the airflow in the inoculation bottle 200 can be released once The air is released to the outside through the third exhaust port 2311, the second exhaust port 202 and the first exhaust port 212; when the disinfection is completed, the knob cover 210 adjusts the relative position of the first exhaust port 212 and the exhaust structure 230. During this process, one end of the sealing piece 232 extending out of the second exhaust port 202 is pressed into the first shell 231 by the cover 210, and the other end of the sealing piece 232 is squeezed and deformed to seal the third exhaust port 2311, thereby completing the pressure and heat preservation sealing of the inoculation bottle 200 after the disinfection is completed, avoiding the mixing of miscellaneous bacteria before inoculation, and the overall sealing effect is good and the operation is convenient.

[0043] Further, such as Figure 5 As shown, the first shell 231 is provided with a limiting rod 233, and the limiting rod 233 is used to limit the range of movement of the blocking member 232. The limiting rod 233 limits the length of the blocking member 232 extending out of the second exhaust port 202, so as to avoid the inability to screw the cover 210 due to the excessive extension length of the blocking member 232. In addition, the setting of the limiting rod 233 can further prevent the blocking member 232 from being misaligned and deformed to block the second exhaust port 202 when releasing steam, or prevent the blocking member 232 from being blown out of the first shell 231 under strong air pressure to cause production accidents.

[0044] Further, such as Figure 5 As shown, the limit rod 233 is inserted into the blocking member 232 along the radial direction of the first shell 231, and the insertion direction of the limit rod 233 is perpendicular to the movement direction of the blocking member 232. The first shell 231 is provided with a groove 2312 for the end of the limit rod 233 to move. The setting of the groove 2312 enables the limit rod 233 to move along with the blocking member 232 while having a certain movement space. Specifically, the side wall of the first shell 231 protrudes outward to form the above-mentioned groove 2312, and the above-mentioned groove 2312 is symmetrically arranged on the two side walls of the first shell 231, so that both ends of the limit rod 233 are restricted in the groove 2312, and the movement range of the end of the limit rod 233 is controlled to achieve the control of the movement range of the blocking member 232.

[0045] In this embodiment, the plugging member 232 is preferably made of one of silicone, rubber, and urethane. Among them, the plugging member 232 made of silicone has a better effect. It should be noted that the end of the plugging member 232 has a spherical surface or a conical surface. Setting the end of the plugging member 232 as a spherical surface or a conical surface is convenient for squeezing the cover body 210 on the one hand, and is also conducive to the plugging member 232 being able to effectively block the third exhaust port 2311 on the other hand.

[0046] In some other embodiments, the ratio of the diameter of the blocking piece 232 to the diameter of the third exhaust port 2311 is greater than 2, so that the blocking piece 232 can effectively block the third exhaust port 2311, and the ratio of the diameter of the blocking piece 232 to the inner diameter of the first shell 231 is between 0.5 and 0.8, so as to leave enough gap for air circulation. The length of the blocking piece 232 is greater than the distance between the second exhaust port 202 and the third exhaust port 2311, so that the blocking piece 232 can extend out of the second exhaust port 202 without blocking the third exhaust port 2311, so that the blocking piece 232 can be controlled to block the third exhaust port 2311 through the cover body 210.

[0047] In some preferred embodiments, in order to further improve the air tightness, a sealing gasket 220 is provided between the cover 210 and the inoculation port 201 , and the sealing gasket 220 may be made of rubber or silicone.

[0048] Furthermore, the cover body 210 is provided with a handle 211, and the handle 211 is hinged or detachably connected to the cover body 210. Specifically, when the handle 211 is hinged to the cover body 210, the handle 211 can be flipped 180°. When the handle 211 is detachably connected to the cover body 210, a connecting protrusion is provided on the cover body 210, and the shape of the protrusion can be one of the polygons. The end of the handle 211 is provided with a hole with a shape corresponding to the protrusion, so that the cover body 210 can be quickly screwed by removing the handle 211. The setting of the handle 211 is convenient for the operator to quickly and labor-savingly screw the cover body 210 without the aid of other tools, thereby improving the disassembly and assembly efficiency.

[0049] like Figure 6As shown, the sterilization and disinfection assembly 400 includes a disinfection ring 410 and an auxiliary rod 430. The auxiliary rod 430 can be bent, and the auxiliary rod 430 is fixedly connected to the disinfection ring 410. The disinfection ring 410 is used to release flames or high-temperature airflow. A fixed rod 110 is provided on the fermenter 100. The auxiliary rod 430 is inserted into the fixed rod 110 or the fermenter 100 so that the disinfection ring 410 is erected outside the inoculation port 201. Specifically, the auxiliary rod 430 can be bent into an L shape or a Z shape to avoid being too long to affect inoculation. In addition, the fixed rod 110 is provided with a sixth fixing piece 360, and the sixth fixing piece 360 ​​is adjustably fixed by a hoop. It is fixed on the fixing rod 110, and the sixth fixing piece 360 ​​can be adjusted along the axial direction of the fixing rod 110. The sixth fixing piece 360 ​​is provided with a through hole for inserting the auxiliary rod 430. The fixing rod 110 is provided with a fastening bolt, which can enter the through hole to resist the auxiliary rod 430, so that the height of the disinfection ring 410 can be adjusted. During vaccination, the handle 211 is removed or set vertically so that the disinfection ring 410 can be adjusted to a suitable position on the inoculation port 201, and then the flame or high-temperature steam is released to sterilize the periphery of the inoculation port 201. When the inoculation is completed, the release of the flame or steam is turned off, and the inoculation is completed. In other embodiments, a seventh fixing member 130 may also be provided on the fermentation tank 100. The seventh fixing member 130 is also provided with a through hole for inserting the auxiliary rod 430, and a fastening bolt that can enter the through hole and support the auxiliary rod 430. Similarly, this method can realize the installation of the disinfection ring 410 outside the inoculation port 201. Compared with the past, there is no need to manually hold the auxiliary rod 430, and the disinfection is more stable and convenient.

[0050] In some embodiments, the fermentation strain inoculation device also includes a second reinforcement component, which is installed on the fixed rod 110, and the second reinforcement component can be adjusted along the axial direction of the fixed rod 110. The second reinforcement component is used to support the disinfection ring 410 so that the disinfection ring 410 can be erected outside the inoculation port 201. The use of the second reinforcement component and the fixed rod 110 can free the operator's hands. At the same time, the use of the sterilization and disinfection component 400 can greatly reduce the mixing of foreign bacteria from before to after inoculation. Specifically, the second reinforcement component includes a fourth fixing member 340 and a fifth fixing member 350. One end of the fourth fixing member 340 has a supporting portion for supporting the disinfection ring 410, and the supporting portion is semi-circular. The other end is fixedly connected to the fifth fixing member 350 by welding, and the fifth fixing member 350 is adjustably connected to the fixing rod 110 in a manner such as a clamp between the second fixing member 320 and the first fixing member 310, so that the height of the second reinforcement component is adjustable, wherein the supporting portion is used to support the disinfection ring 410 to free the operator's hands and ensure that the disinfection process is stable. The adjustable height of the second reinforcement component also facilitates the height adjustment of the disinfection ring 410.

[0051] like Figure 6 and Figure 7 In one embodiment shown, a disinfection ring 410 is provided with a groove, in which a fuel core 420 is arranged, and the fuel core 420 is used to absorb alcohol. When it is necessary to disinfect the bacteria in the inoculation port 201, igniting the fuel core 420 can cause the disinfection ring 410 to release a high-temperature flame. Specifically, the disinfection ring 410 is provided with an annular groove for placing the fuel core 420. The annular disinfection ring 410 facilitates all-round high-temperature open flame disinfection of the outer wall of the inoculation port 201 and the cover body 210 without dead ends, so that during the inoculation process, the outer wall of the inoculation port 201, the cover body 210 and the nearby airflow can also be disinfected. In addition, the disinfection ring 410 not only supports the fuel core 420, but also controls the combustion range of the fuel core 420.

[0052] Furthermore, the sterilization and disinfection component 400 also includes a fire extinguishing cover 440, which is used to cover the disinfection ring 410. When the inoculation is completed, the disinfection ring 410 can be covered by the fire extinguishing cover 440 so that the open flame on the combustion core 420 is extinguished, thereby achieving the purpose of fire extinguishing.

[0053] In other embodiments, a plurality of release holes 411 are arranged in an array on the side wall of the annular groove. The plurality of release holes 411 arranged in an array on the side wall of the annular groove can help control the speed at which air enters the annular groove, thereby controlling the burning amplitude of the flame. At the same time, when the alcohol in the wick 420 is consumed or scrapped, it is also convenient to quickly replace it. In other embodiments, the wick 420 can also be directly wrapped around the outside of the disinfection ring 410.

[0054] like Figure 8 In another embodiment shown, the interior of the disinfection ring 410 is hollow, and the inner side and the upper side of the disinfection ring 410 are arranged in an array with release holes 411. The disinfection ring 410 is also provided with a gas connector 412 for connecting to an external gas source. When it is not necessary to open the cover body 210 for bacterial inoculation, the gas connector 412 is connected to an external steam generating device, and the steam from the generating device enters the disinfection ring 410 and is sprayed to the periphery of the inoculation port 201 through the release holes 411. Fig. 9 As shown, during inoculation, the disinfection ring 410 is first started to release steam, and then the first exhaust port 212 on the cover body 210 is rotated to face the second exhaust port 202 through the handle 211, and the delivery tube 510 of the shaking bottle 500 enters the inoculation bottle 200 through the first exhaust port 212 and the second exhaust port 202 for inoculation of the bacteria. Generally, the delivery tube 510 is a silicone hose. Compared with the use of open flames to control the disinfecting of miscellaneous bacteria, the high-temperature steam sterilization method can better protect the delivery tube 510. For the catheter-type access of the shaking bottle and the inoculation bottle to introduce the seed liquid into the inoculation bottle, only the connected catheter and its surrounding areas are subjected to high-temperature and high-pressure steam sterilization of miscellaneous bacteria, so as to achieve effective sterilization in a local range without damaging the catheter.

[0055] Further, such as Figure 6 and Figure 7 As shown, the auxiliary rod 430 includes a first connecting segment 431, a second connecting segment 432 and a third connecting segment 433, one end of the first connecting segment 431 is fixedly connected to the disinfection ring 410, the other end of the first connecting segment 431 is connected to one end of the second connecting segment 432 through a first limiting structure, and the other end of the second connecting segment 432 is connected to the third connecting segment 433 through a second limiting structure, wherein, under the action of the first limiting structure, the second connecting segment 432 has a first state and a second state, in the first state, the first connecting segment 431 and the second connecting segment 432 are collinear, and in the second state, the first connecting segment 431 and the second connecting segment 432 are bent at a certain angle, such as vertically; under the action of the second limiting structure, the third connecting segment 433 has a third state and a fourth state, in the third state, the second connecting segment 432 and the third connecting segment 433 are collinear, and in the fourth state, the second connecting segment 432 and the third connecting segment 433 are bent at a certain angle, such as vertically. When the second connecting segment 432 is in the second state and the third connecting segment 433 is in the fourth state, the auxiliary rod 430 can be bent into an L shape, or, when the second connecting segment 432 is in the first state and the third connecting segment 433 is in the fourth state, the auxiliary rod 430 can also be bent into an L shape; further, when the second connecting segment 432 is in the second state and the third connecting segment 433 is in the fourth state, the auxiliary rod 430 can be bent into an L shape or a U shape, which is convenient for fixing or adjusting the auxiliary rod 430, so that the auxiliary rod 430 can be set on the fixed rod 110 or the fermentation tank 100.

[0056] In a preferred embodiment of the present application, in the second state, the first connecting segment 431 is perpendicular to the second connecting segment 432 , and in the fourth state, the second connecting segment 432 is perpendicular to the third connecting segment 433 .

[0057] Further, the first limiting structure includes a first groove 4311, a first connecting portion 4321 and a first adjusting knob 4313, wherein the first groove 4311 is provided in the first connecting segment 431, the first connecting portion 4321 is fixed to the second connecting segment 432, the first connecting portion 4321 is hinged to the first groove 4311 through a first hinge 4312, so that the first connecting portion 4321 can be turned over a certain angle with the first hinge 4312 as the rotation center, the first adjusting knob 4313 is provided in the first connecting segment 431 and can enter the first groove 4311 to limit the movement of the first connecting portion 4321, and the second connecting segment 432 can be switched between the first state and the second state through the first adjusting knob 4313. The adjustment is convenient, and the auxiliary rod 430 can be quickly bent and adjusted.

[0058] Further, the first adjusting knob 4313 is threadedly connected to the first connecting section 431, and the first connecting portion 4321 is provided with a first limiting hole 4321a. In the first state, the first adjusting knob 4313 is located in the first limiting hole 4321a, and in the second state, one side of the first connecting portion 4321 abuts against the bottom of the first groove 4311, and the end of the first adjusting knob 4313 abuts against the other side of the first connecting portion 4321. The restriction on the first connecting portion 4321 is released by rotating the first adjusting knob 4313, so that the second connecting section 432 can be freely rotated, and then rotated to a specific position and restricted by the first adjusting knob 4313, so that the second connecting section 432 can switch between the first state and the second state.

[0059] Further, the second limiting structure includes a second groove 4322, a second connection part 4331 and a second adjusting knob 4324, wherein the second groove 4322 is provided in the second connection section 432, the second connection part 4331 is fixed to the third connection section 433, the second connection part 4331 is hinged to the second groove 4322 through a second hinge 4323, so that the second connection part 4331 can be turned over a certain angle with the second hinge 4323 as the rotation center, the second adjusting knob 4324 is provided in the second connection section 432 and can enter the second groove 4322 to limit the movement of the second connection part 4331, and the third connection section 433 can be switched between the second state and the third state through the second adjusting knob 4324. The adjustment is convenient, and the auxiliary rod 430 can be quickly bent and adjusted.

[0060] Further, the second adjusting knob 4324 is threadedly connected to the second connecting section 432, and the second connecting portion 4331 is provided with a second limiting hole 4331a. In the third state, the end of the second adjusting knob 4324 is located at the second limiting hole 4331a. In the fourth state, one side of the second connecting portion 4331 abuts against the bottom of the second groove 4322, and the end of the second adjusting knob 4324 abuts against the other side of the second connecting portion 4331. The restriction on the second connecting portion 4331 is released by rotating the second adjusting knob 4324, so that the third connecting section 433 can be freely rotated, and then rotated to a specific position and restricted by the second adjusting knob 4324, so that the third connecting section 433 can switch between the third state and the fourth state.

[0061] Furthermore, the wick 420 is made of absorbent cotton and gauze, wherein the absorbent cotton refers to cotton that has been chemically treated to remove fat, and is made from raw cotton that has been removed of inclusions, degreased, bleached, washed, dried, and finished. It is easier to absorb liquid than ordinary cotton, and is less likely to leak after adsorption. When the wick 420 is made, the absorbent cotton is rolled into strips, and then the strips of absorbent cotton are covered with a layer of gauze to form the above-mentioned wick 420. When in use, the wick 420 soaked in alcohol is placed in a disinfection ring 410 with a groove.

[0062] In addition, the distance between the inner wall of the disinfection ring 410 and the outer wall of the inoculation port 201 is controlled at 10 to 20 mm. If the disinfection ring 410 is set too far away, it is not conducive to the disinfection of miscellaneous bacteria, and if it is too close, it is easy to cause damage to the inoculated bacteria during inoculation.

[0063] In some other embodiments, such as Figure 1 and Figure 2 As shown, an adjustable first reinforcement component is provided on the fixing rod 110, and the first reinforcement component includes a first fixing part 310, a second fixing part 320, and a third fixing part 330. One end of the first fixing part 310 has a semi-annular structure, and the other end is fixedly connected to the third fixing part 330. The third fixing part 330 is fixed to the fixing rod 110 by means of a clamp, so that the first reinforcement component can be adjusted up and down, wherein the two ends of the semi-annular structure on the second fixing part 320 have through holes, and the second fixing part 320 is also semi-annular, and the two ends of the second fixing part 320 also have through holes. After the second fixing part 320 is docked with the semi-annular structure on the first fixing part 310, the inoculation bottle 200 can be clamped, and then the second fixing part 320 is fixedly connected to the first fixing part 310 by bolts and nuts, so that the inoculation bottle 200 can be further reinforced, and the combination of the second fixing part 320 and the first fixing part 310 can adapt to the fixation of inoculation bottles 200 of different sizes.

[0064] The following will introduce the fermentation strain inoculation process of this application in detail based on the above content:

[0065] Before inoculation, the cover body 210 is first threadedly connected to the inoculation port 201, and the first exhaust port 212 is misaligned with the second exhaust port 202 to ensure that the inoculation bottle 200 is not connected to the outside world, and then the diaphragm valve 121 is opened to release the high-temperature steam in the fermentation tank 100 into the inoculation bottle 200 through the connecting pipe 120. After a period of time, the operator screws the cover body 210 through the handle 211 to make the first exhaust port 212 face the second exhaust port 202. At this time, the blocking member 232 returns to its original state and releases the blocking of the third exhaust port 2311 under the action of air pressure. The high-temperature steam is released from the third exhaust port 2311 to the outside world via the first exhaust port 212 and the second exhaust port 202, and at the same time, the inside and outside of the cover body 210 and the inside of the inoculation port 201 are The first exhaust port 212 and the second exhaust port 202 are staggered, and the operator screws the cover 210 with the handle 211 to displace the first exhaust port 212 and the second exhaust port 202, so as to quickly keep the inoculation bottle 200 warm and pressurized. After this process is completed, the burning core 420 full of alcohol is ignited and placed on the supporting part, so that the sterilization and disinfection component 400 can stably disinfect the inoculation port 201 and the cover 210 with continuous open flame and high temperature. During inoculation, the operator screws open the cover 210 with the handle 211, puts the bacteria to be inoculated into the inoculation bottle 200 from the inoculation port 201, and then screws the cover 210 back to the inoculation port 201 after the open flame disinfection. At this point, the inoculation is completed, and the open flame on the disinfection ring 410 is extinguished by the fire extinguishing cover 440. Compared with the prior art, the fermentation bacteria inoculation device of the present application is reasonably designed, can effectively disinfect the components of the inoculation part of the device, and can also avoid the mixing of miscellaneous bacteria before and during inoculation.

[0066] The embodiments of the present invention are described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above embodiments. Various changes can be made within the knowledge scope of ordinary technicians in the technical field without departing from the purpose of the present invention.

Claims

1. A fermentation bacteria inoculation device, characterized in that: include Fermentation tank (100); An inoculation bottle (200) is fixed to the fermentation tank (100) and communicates with the interior of the fermentation tank (100); a cover body (210) detachably connected to the inoculation port (201) of the inoculation bottle (200), wherein the cover body (210) has a first exhaust port (212), and the first exhaust port (212) is configured to control the communication between the interior of the inoculation bottle (200) and the outside; and A sterilization and disinfection component (400) is installed outside the inoculation port (201). The sterilization and disinfection component (400) includes a disinfection ring (410) and an auxiliary rod (430). The auxiliary rod (430) can be bent. The auxiliary rod (430) is fixedly connected to the disinfection ring (410). The disinfection ring (410) is used to release flames or high-temperature airflow.

2. The fermentation bacteria inoculation device according to claim 1, characterized in that: The cover body (210) is threadedly connected to the inoculation port (201), the first exhaust port (212) is arranged on the side of the cover body (210), the inoculation port (201) is provided with an exhaust structure (230), and the exhaust structure (230) includes a second exhaust port (202), wherein the first exhaust port (212) can be rotated to face the second exhaust port (202).

3. The fermentation bacteria inoculation device according to claim 1, characterized in that: The fermentation tank (100) is provided with a connecting pipe (120) for connecting with the inoculation bottle (200), and a diaphragm valve (121) is provided on the connecting pipe (120).

4. The fermentation bacteria inoculation device according to claim 1, characterized in that: The fermentation tank (100) is provided with a fixing rod (110), and the auxiliary rod (430) is inserted into the fixing rod (110) or the fermentation tank (100), so that the disinfection ring (410) is set outside the inoculation port (201).

5. The fermentation bacteria inoculation device according to claim 4, characterized in that: The disinfection ring (410) is provided with a groove, a combustion core (420) is arranged in the groove, and the combustion core (420) is used to absorb alcohol.

6. The fermentation bacteria inoculation device according to claim 4, characterized in that: The interior of the disinfection ring (410) is hollow, and release holes (411) are arranged in an array on the inner side and the upper side of the disinfection ring (410). The disinfection ring (410) is also provided with a gas connector (412) for connecting to an external gas source.

7. The fermentation bacteria inoculation device according to claim 4, characterized in that: The auxiliary rod (430) includes a first connecting section (431), a second connecting section (432) and a third connecting section (433), one end of the first connecting section (431) is fixedly connected to the disinfection ring (410), the other end of the first connecting section (431) is connected to one end of the second connecting section (432) via a first limiting structure, and the other end of the second connecting section (432) is connected to the third connecting section (433) via a second limiting structure, wherein Under the action of the first limiting structure, the second connecting section (432) has a first state and a second state. In the first state, the first connecting section (431) and the second connecting section (432) are collinear. In the second state, the first connecting section (431) and the second connecting section (432) are bent at a certain angle. Under the action of the second limiting structure, the third connecting segment (433) has a third state and a fourth state. In the third state, the second connecting segment (432) and the third connecting segment (433) are collinear. In the fourth state, the second connecting segment (432) and the third connecting segment (433) are bent at a certain angle.

8. The fermentation bacteria inoculation device according to claim 7, characterized in that: In the second state, the first connecting section (431) is perpendicular to the second connecting section (432); in the fourth state, the second connecting section (432) is perpendicular to the third connecting section (433).

9. The fermentation bacteria inoculation device according to claim 8, characterized in that: The first limiting structure includes a first groove (4311), a first connecting portion (4321) and a first adjusting knob (4313), wherein the first groove (4311) is arranged on the first connecting section (431), the first connecting portion (4321) is fixed to the second connecting section (432), the first connecting portion (4321) is hinged to the first groove (4311), the first adjusting knob (4313) is arranged on the first connecting section (431) and can enter the first groove (4311) to limit the movement of the first connecting portion (4321), and the second connecting section (432) can be switched between the first state and the second state through the first adjusting knob (4313).

10. The fermentation bacteria inoculation device according to claim 9, characterized in that: The first adjusting knob (4313) is threadedly connected to the first connecting section (431), and the first connecting portion (4321) is provided with a first limiting hole (4321a). In the first state, the first adjusting knob (4313) is located in the first limiting hole (4321a). In the second state, one side of the first connecting portion (4321) abuts against the bottom of the first groove (4311), and the end of the first adjusting knob (4313) abuts against the other side of the first connecting portion (4321).

11. The fermentation bacteria inoculation device according to claim 8, characterized in that: The second limiting structure includes a second groove (4322), a second connecting portion (4331) and a second adjusting knob (4324), wherein the second groove (4322) is arranged on the second connecting section (432), the second connecting portion (4331) is fixed to the third connecting section (433), the second connecting portion (4331) is hinged to the second groove (4322), the second adjusting knob (4324) is arranged on the second connecting section (432) and can enter the second groove (4322) to limit the movement of the second connecting portion (4331), and the third connecting section (433) can be switched between the second state and the third state through the second adjusting knob (4324).

12. The fermentation bacteria inoculation device according to claim 11, characterized in that: The second adjusting knob (4324) is threadedly connected to the second connecting section (432), and the second connecting portion (4331) is provided with a second limiting hole (4331a). In the third state, the end of the second adjusting knob (4324) is located in the second limiting hole (4331a). In the fourth state, one side of the second connecting portion (4331) abuts against the bottom of the second groove (4322), and the end of the second adjusting knob (4324) abuts against the other side of the second connecting portion (4331).

13. The fermentation bacteria inoculation device according to claim 2, characterized in that: The exhaust structure (230) includes a first shell (231) and a blocking member (232), wherein one end of the first shell (231) is open and connected to the second exhaust port (202), and the other end of the first shell (231) has a third exhaust port (2311), and the blocking member (232) is movably arranged in the first shell (231), and the blocking member (232) blocks the third exhaust port (2311) under the action of the cover body (210), wherein when the second exhaust port (202) is aligned with the first exhaust port (212), the blocking member (232) can extend from the second exhaust port (202) to the outside of the inoculation port (201).

14. The fermentation bacteria inoculation device according to claim 13, characterized in that: The first shell (231) is provided with a limiting rod (233), and the limiting rod (233) is used to limit the range of movement of the blocking member (232).

15. The fermentation bacteria inoculation device according to claim 14, characterized in that: The limiting rod (233) is inserted into the blocking member (232) along the radial direction of the first shell (231), and the first shell (231) is provided with a groove (2312) for the end of the limiting rod (233) to move.

16. The fermentation bacteria inoculation device according to claim 13, characterized in that: The blocking member (232) is one of silica gel, rubber and urethane foam, and the end of the blocking member (232) has a spherical surface or a conical surface.

17. The fermentation bacteria inoculation device according to claim 1, characterized in that: The cover body (210) is provided with a handle (211), and the handle (211) is hingedly or detachably connected to the cover body (210).

Images