Quantitative inoculation device for liquid strains

Abstract

The utility model discloses a liquid strain quantitative inoculation device which comprises a device body and a strain storage bottle, the device body is provided with a storage chamber, a piston, a piston push rod, an operation chamber, a piston push rod sealing sleeve and an inoculation needle tube, one end of the storage chamber is connected with the operation chamber, and the other end of the storage chamber is connected with the inoculation needle tube. One side of the storage chamber is connected with the strain storage bottle through the liquid guide pipe, the piston is arranged in the operation chamber, the piston push rod is connected with the piston, and the piston push rod sealing sleeve is connected with the operation chamber. According to the utility model, the strain culture bottle is connected with the device main body, so that the infection risk caused by rotating the strain culture bottle is avoided; meanwhile, the strain culture risk can be effectively reduced by adopting the small bottle to culture the strain. The automatic inoculation device can be used in cooperation with an automatic pushing system, automatic inoculation is achieved, and the inoculation efficiency is higher.

Description

Technical Field

[0001] The utility model belongs to the technical field of live bacteria inoculation, and particularly relates to a liquid bacteria quantitative inoculation device. Background Art

[0002] The factory cultivation of edible fungi is the most modern agricultural industrialized production method. At present, factory production will enter a mature and rapid development stage. As one of the existing inoculation methods, handheld inoculation has the following problems in the inoculation process: low automation, difficult to upgrade to an automatic inoculation gun, high labor intensity, low production efficiency; manual trigger inoculation easily leads to inconsistent inoculation amount, resulting in poor consistency of the fungus bag's germination state. Therefore, a quantitative inoculation device that can be quantitatively inoculated and can be upgraded to automatic inoculation is needed to improve the success rate and efficiency of inoculation.

[0003] The patent document with publication number CN207811746U discloses a gun-type simple liquid addition and liquid strain inoculation device, including a liquid storage bottle, a liquid drawing device and a gun-type operating device, the liquid storage bottle includes a bottle cap, a sterile vent plug and a bottle body, the liquid storage bottle is inserted and installed with a liquid drawing device, the liquid drawing device includes a lower hose, a hard connector and an upper hose, and the gun-type operating device includes a trigger switch, a liquid storage bubble, an upper squeeze plate, a lower squeeze plate, a connecting hose and a metal inoculation head. The device is versatile and can realize the subpackaging of test tubes and conical flasks, the addition of bottle washing distilled water, the addition and quantification of multiple liquids, and the transfer of liquid strains. However, it is difficult to upgrade the automatic inoculation function of the device. Utility Model Content

[0004] In order to solve the above technical problems, the utility model provides a liquid strain quantitative inoculation device, which can solve the problem of inconsistent inoculation amounts in manual inoculation and difficulty in upgrading to automatic inoculation.

[0005] The utility model is realized through the following technical solutions.

[0006] The utility model provides a liquid strain quantitative inoculation device, comprising a device body and a strain storage bottle, wherein a storage chamber, a piston, a piston push rod, an operating chamber, a piston push rod sealing sleeve and an inoculation needle are arranged on the device body, one end of the storage chamber is connected to the operating chamber, the other end of the storage chamber is connected to the inoculation needle, one side of the storage chamber is connected to the strain storage bottle through a liquid guide tube, the piston is arranged in the operating chamber, the piston push rod is connected to the piston, and the piston push rod sealing sleeve is connected to the operating chamber.

[0007] Preferably, a piston push rod fixing part, a piston brake connecting rod and a piston brake handle are provided on the piston push rod. The piston push rod is connected to the piston push rod fixing part after passing through the piston push rod sealing sleeve. One end of the piston brake connecting rod is connected to the piston push rod fixing part, and the other end of the piston brake connecting rod is connected to the piston brake handle.

[0008] Preferably, one end of the inoculation needle is connected to the storage chamber through an inoculation needle check valve, a mushroom bag opening cone is provided at the other end of the inoculation needle, and a gun body holding handle is provided on the device body.

[0009] Preferably, the diameter of the mushroom bag hole opening cone head is larger than the diameter of the inoculation needle tube, a sharp point is provided at the end of the mushroom bag hole opening cone head, and the connection between the mushroom bag hole opening cone head and the inoculation needle tube is in an inverted arc shape.

[0010] Preferably, an elastic member is arranged on the piston push rod, and the elastic member is arranged on the piston push rod sealing sleeve section.

[0011] Preferably, a strain storage bottle fixing piece is provided at the opening of the strain storage bottle, a gasket is provided inside the strain storage bottle fixing piece, and the liquid guide tube penetrates through the strain storage bottle fixing piece and then extends into the strain storage bottle.

[0012] Preferably, a piston limiting spacer is arranged between the storage chamber and the operation chamber, a vent hole is arranged on the piston limiting spacer, and a liquid guiding tube check valve is arranged on the liquid guiding tube.

[0013] Preferably, the piston brake handle is arranged in a curved shape in the middle.

[0014] Preferably, a sealing assembly is provided in the operating chamber.

[0015] Preferably, a piston push rod adjusting nut is provided at one end of the piston push rod sealing sleeve.

[0016] The beneficial effects of the utility model are:

[0017] 1. The utility model seals the push rod through the piston push rod sealing sleeve, effectively preventing the push rod from bringing foreign bacteria into the device body when repeatedly pushed, causing inoculation contamination, and effectively improving the inoculation success rate.

[0018] 2. Utilizing the air pressure principle, the piston is prevented from contacting the bacteria during the process of sucking the bacteria into the storage chamber and then squeezing the bacteria out, effectively avoiding contact and contamination of objects during inoculation.

[0019] 3. The utility model can complete puncture and inoculation simultaneously, effectively reducing the inoculation intensity and improving the inoculation speed and efficiency. If a breathable film is pasted on the inoculation port after the inoculation is completed, the inoculation effect will be better. Since the inoculation speed of the utility model is fast and the contact time between the inoculation port and the outside world is short, the probability of infection due to the influence of the culture environment after inoculation will be smaller.

[0020] 4. The utility model adopts a setting mode of connecting the culture bottle of bacterial strains with the main body of the device, which avoids the risk of infection caused by transferring the bacteria to the bottle; at the same time, the use of small bottles to culture bacterial strains can also effectively reduce the risk of bacterial strain culture. The utility model can also be used in conjunction with an automatic propulsion system to achieve automatic inoculation, and the inoculation efficiency will be higher. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a structural schematic diagram of the utility model;

[0022] In the figure: 1-storage chamber, 2-piston limit spacer, 3-piston, 4-piston push rod, 5-operating chamber, 6-sealing assembly, 7-elastic member, 8-piston push rod sealing sleeve, 9-piston push rod adjusting nut, 10-piston push rod fixing piece, 11-piston brake connecting rod, 12-inoculation needle tube check valve, 13-inoculation needle tube, 14-bacteria bag opening cone head, 15-liquid guide tube, 16-liquid guide tube check valve, 17-bacteria culture storage bottle fixing piece, 171-gasket, 18-bacteria culture storage bottle, 19-piston brake handle, 20-gun body holding handle. DETAILED DESCRIPTION

[0023] The technical solution of the utility model is further described below, but the scope of protection required is not limited to the description.

[0024] Example:

[0025] like Figure 1 As shown, a liquid bacterial strain quantitative inoculation device includes a device body and a bacterial strain storage bottle 18, wherein a storage chamber 1, a piston 3, a piston push rod 4, an operating chamber 5, a piston push rod sealing sleeve 8 and an inoculation needle 13 are arranged on the device body, wherein one end of the storage chamber 1 is connected to the operating chamber 5, and the other end of the storage chamber 1 is connected to the inoculation needle 13, and one side of the storage chamber 1 is connected to the bacterial strain storage bottle 18 via a liquid guide tube 15, the piston 3 is arranged in the operating chamber 5, the piston push rod 4 is connected to the piston 3, and the piston push rod sealing sleeve 8 is connected to the operating chamber 5.

[0026] The main function of the storage chamber 1 is to store the inoculation amount of a single bacterial strain inoculation. The main function of the piston 3 is to change the air pressure inside the device body, suck the bacteria from the bacterial strain storage bottle 18 into the bacterial strain storage chamber 1, and squeeze and spray the bacteria in the bacterial strain storage chamber 1 from the inoculation needle 13 to complete the inoculation. The main function of the piston push rod 4 is to push and pull the piston 3 up and down. The main function of the operating chamber 5 is to provide a fixed stroke and a clean internal space for the piston 3. The piston push rod sealing sleeve 8 is a sealing rubber sleeve, the lower part of which is connected to the outside of the sealing component 6, and the upper part is connected to the outside of the piston push rod adjusting nut 9. The piston push rod sealing sleeve 8 seals and wraps the piston push rod 4 and the piston return spring 7; the main function of the piston push rod sealing sleeve 8 is to isolate the outside air, ensure that the operating space of the piston push rod 4 is clean, and prevent external bacteria from contaminating. The bacterial strain storage bottle 18 can also be a bacterial strain culture bottle to improve the inoculation effect.

[0027] The piston push rod 4 is provided with a piston push rod fixing part 10, a piston brake connecting rod 11 and a piston brake handle 19. The piston push rod 4 passes through the piston push rod sealing sleeve 8 and is connected to the piston push rod fixing part 10. One end of the piston brake connecting rod 11 is connected to the piston push rod fixing part 10, and the other end of the piston brake connecting rod 11 is connected to the piston brake handle 19.

[0028] The piston push rod fixing part 10 is installed on the top of the piston push rod 4, and the side opening is connected to the piston brake connecting rod 11. The main function of the piston push rod fixing part 10 is to connect the piston push rod 4 and the piston brake connecting rod 11 to push the piston 3 to run. The lower part of the piston brake connecting rod 11 is connected to the piston brake handle 19, and the upper part is connected to the piston push rod fixing part 10. The upper part is installed with an adjusting nut for adjusting the running distance of the piston brake connecting rod 11 to adapt to different users.

[0029] One end of the inoculation needle tube 13 is connected to the storage chamber 1 through an inoculation needle tube check valve 12, and the other end of the inoculation needle tube 13 is provided with a mushroom bag hole opening cone head 14. The device body is provided with a gun body holding handle 20, which mainly functions to be used for holding the device and assisting in pulling the piston brake handle 19, so as to assist in inserting the mushroom bag hole opening cone head 14 into the mushroom bag to complete the inoculation point opening.

[0030] The main function of the inoculation needle check valve 12 is to prevent external air from entering the culture storage chamber 1 from the inoculation needle 13 when the piston 3 moves upward after the inoculation is completed, so as to prevent the culture from being sucked into the culture storage chamber 1 from the culture storage bottle 18, or the amount of sucked culture does not reach the specified inoculation amount. The inoculation needle 13 is provided with a tube wall hole, and the tube wall hole can be opened at the corresponding position according to the inoculation requirements; the main function is to fix the hole cone head 14 of the fungus bag, and spray the culture from the tube wall hole to complete the inoculation task.

[0031] The diameter of the mushroom bag hole-opening cone head 14 is larger than the diameter of the inoculation needle tube 13. This arrangement makes the diameter of the inoculation hole larger than the tube diameter of the inoculation needle tube 13, which is conducive to the spraying of bacteria and preventing the culture medium from clogging the tube wall hole. The main function of the mushroom bag hole-opening cone head 14 is to open the inoculation hole on the inoculated mushroom bag. A sharp point is arranged at the end of the mushroom bag hole-opening cone head 14 to help insert the inoculation needle tube 13 into the tube wall hole to achieve the purpose of fixation. The connection between the mushroom bag hole-opening cone head 14 and the inoculation needle tube 13 is in an inverted arc shape, so as to facilitate pulling it out of the mushroom bag after the inoculation is completed.

[0032] The piston push rod 4 is provided with an elastic member 7, which is provided on the piston push rod 4 at the piston push rod sealing sleeve 8. The elastic member 7 is a piston return spring, the lower part of the elastic member 7 is connected to the sealing assembly 6, and the upper part of the elastic member 7 is connected to the piston push rod adjusting nut 9. The main function of the elastic member 7 is to push the piston 3 upward to the original position after each inoculation, and at the same time, to absorb the strains into the strain storage chamber 1, in preparation for the next inoculation.

[0033] A strain storage bottle fixing part 17 is arranged at the opening of the strain storage bottle 18, and a simple locking device or a thread is arranged on the inner wall of the strain storage bottle fixing part 17, which is used to fix the bottle cap of the strain storage bottle 18 and ensure the sealing of the connection between the strain liquid guide tube 15 and the strain storage bottle 18. A gasket 171 is arranged in the strain storage bottle fixing part 17, and the gasket 171 is an elastic rubber gasket. The gasket 171 is used to seal the gap between the strain storage bottle fixing part 17 and the bottle cap of the strain storage bottle 18. The liquid guide tube 15 penetrates the strain storage bottle fixing part 17 and extends into the strain storage bottle 18.

[0034] The left end of the liquid guide tube 15 is connected to the culture storage chamber 1 , and the other end extending into the culture storage bottle 18 is triangularly pointed and convenient for inserting into the culture storage bottle 18 , so as to transport the liquid culture in the culture storage bottle 18 to the culture storage chamber 1 in a directionally manner.

[0035] A piston limit spacer 2 is arranged between the storage chamber 1 and the operating chamber 5, and the periphery of the piston limit spacer 2 is seamlessly connected to the tube body. A vent hole is arranged on the piston limit spacer 2, and its main function is to limit the contact between the piston 3 and the bacteria. At the same time, it can also prevent the bacteria from entering the operating chamber 5 due to the tilt of the gun body during inoculation, causing the bacteria to contact the piston 3 and affect the inoculation quality. A liquid guide tube check valve 16 is arranged on the liquid guide tube 15 to prevent the liquid bacteria from flowing back from the bacteria storage chamber 1 to the bacteria storage bottle 18.

[0036] The piston brake handle 19 is bent in the middle, and the bent portion of the piston brake handle 19 is installed on the gun body grip handle 20 as a lever fulcrum, and the other end is connected to the piston brake connecting rod 11. By pressing the end of the piston brake handle 19 away from the device body, the other end moves toward the inoculation needle tube 13, so that the piston 3 is pulled by hand to complete the inoculation action. Pressing the piston brake handle 19 once fixes the movement distance of the piston 3, thereby achieving quantitative inoculation.

[0037] A sealing assembly 6 is provided in the operating chamber 5, and the sealing assembly 6 is installed on the upper part of the operating chamber 5. The periphery of the sealing assembly 6 is fixed and sealed with the operating chamber 5 through threads and rubber rings. The sealing assembly 6 has a hole in the middle and contacts with the piston push rod 4 through the sealing rubber ring to ensure smooth operation of the piston push rod 4. The main function of the sealing assembly 6 is to prevent particulate matter in the external air from entering the interior of the operating chamber 5.

[0038] A piston push rod adjusting nut 9 is arranged at one end of the piston push rod sealing sleeve 8. The piston push rod adjusting nut 9 is installed on the upper part of the piston push rod 4, the lower part of the piston push rod adjusting nut 9 is connected to the elastic member 7, and the outside of the piston push rod adjusting nut 9 is connected to the piston push rod sealing sleeve 8. The main function of the piston push rod adjusting nut 9 is to adjust the travel distance of the piston push rod 4, so as to achieve the purpose of adjusting the inoculation amount.

[0039] After the piston brake handle 19, the gun body holding handle 20 and the piston brake connecting rod 11 are removed, the utility model can install multiple device bodies on the automatic pressing device according to production needs and equip them with linked pressing piston push rods to realize the functions of automatic vaccination and one-time multi-point automatic vaccination.

Claims

1. A liquid bacterial quantitative inoculation device, characterized in that: The invention comprises a device body and a strain storage bottle (18); the device body is provided with a storage chamber (1), a piston (3), a piston push rod (4), an operating chamber (5), a piston push rod sealing sleeve (8) and an inoculation needle tube (13); one end of the storage chamber (1) is connected to the operating chamber (5), the other end of the storage chamber (1) is connected to the inoculation needle tube (13); one side of the storage chamber (1) is connected to the strain storage bottle (18) via a liquid guide tube (15); the piston (3) is arranged in the operating chamber (5), the piston push rod (4) is connected to the piston (3), and the piston push rod sealing sleeve (8) is connected to the operating chamber (5).

2. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: The piston push rod (4) is provided with a piston push rod fixing part (10), a piston brake connecting rod (11) and a piston brake handle (19); the piston push rod (4) passes through the piston push rod sealing sleeve (8) and is connected to the piston push rod fixing part (10); one end of the piston brake connecting rod (11) is connected to the piston push rod fixing part (10), and the other end of the piston brake connecting rod (11) is connected to the piston brake handle (19).

3. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: One end of the inoculation needle tube (13) is connected to the storage chamber (1) via an inoculation needle tube check valve (12), and a mushroom bag opening cone head (14) is provided at the other end of the inoculation needle tube (13). A gun body holding handle (20) is provided on the device body.

4. A liquid bacterial strain quantitative inoculation device as claimed in claim 3, characterized in that: The diameter of the mushroom bag hole opening cone (14) is larger than the diameter of the inoculation needle tube (13), a sharp point is arranged at the end of the mushroom bag hole opening cone (14), and the connection between the mushroom bag hole opening cone (14) and the inoculation needle tube (13) is in an inverted arc shape.

5. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: An elastic member (7) is arranged on the piston push rod (4), and the elastic member (7) is arranged on the section of the piston push rod (4) located on the piston push rod sealing sleeve (8).

6. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: A strain storage bottle fixing piece (17) is arranged at the opening of the strain storage bottle (18), a gasket (171) is arranged inside the strain storage bottle fixing piece (17), and the liquid guide tube (15) penetrates the strain storage bottle fixing piece (17) and then extends into the strain storage bottle (18).

7. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: A piston limiting spacer (2) is arranged between the storage chamber (1) and the operation chamber (5), a vent hole is arranged on the piston limiting spacer (2), and a liquid guiding tube check valve (16) is arranged on the liquid guiding tube (15).

8. A liquid bacterial strain quantitative inoculation device as claimed in claim 2, characterized in that: The piston brake handle (19) is arranged in a bent shape in the middle.

9. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: A sealing component (6) is arranged in the operating chamber (5).

10. A liquid bacterial strain quantitative inoculation device as claimed in claim 1, characterized in that: A piston push rod adjusting nut (9) is arranged at one end of the piston push rod sealing sleeve (8).

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