Cell culture device for researching fluid shear force

Abstract

The utility model discloses a cell culture device for studying fluid shear force, which comprises a control box, a peristaltic pump, a liquid storage bottle, a damping module, a fluid chamber and a plurality of glass slides, the peristaltic pump is installed on the control box, the peristaltic pump is communicated with the liquid storage bottle, the liquid storage bottle is communicated with the fluid chamber, the fluid chamber is communicated with the damping module, and the damping module is installed on the control box. The damping module is communicated with the peristaltic pump, a plurality of flow grooves are formed in the fluid cavity, the glass slides are installed on the flow grooves, the damping module comprises a plurality of pulse dampers, and the pulse dampers are connected in series. The fluid shear force loading device is small in size, fluid shear force loading can be carried out in a constant-temperature cell culture box, the research threshold of fluid shear force is lowered, and development of fluid shear force research can be better promoted.

Description

Technical Field

[0001] The invention relates to a cell culture device for studying fluid shear force. Background Art

[0002] Cells in the human body are always in a biomechanical microenvironment, and fluid shear force is one of the important forms of mechanical action. Under physiological conditions, many cell types are in a fluid environment, especially cells such as vascular endothelial cells and lymphatic endothelial cells. Taking vascular endothelial cells as an example, the flow of blood will produce shear stress on vascular endothelial cells. The existence of this mechanical stress can affect various biological behaviors and functions of cells, such as growth, adhesion, differentiation, aging and migration. On the contrary, if cells that are stimulated by fluid shear force for a long time lose this mechanical effect, they will gradually show a pathological state. Therefore, revealing the mechanism by which various types of cells sense the action of fluid shear force is the key to better understanding the physiological functions of cells and optimizing and improving clinical treatment strategies.

[0003] However, most current cell studies are conducted in a static state, and the mechanical environment is not maintained for cell types such as vascular endothelial cells that are constantly stimulated by fluid shear forces. Therefore, it is difficult for the cell state to truly simulate the physiological conditions in vivo, and the results of in vitro cell experiments are difficult to verify in the in vivo fluid environment. To solve this problem, there are currently a wide variety of fluid shear force loading devices, all of which load fluid shear force on cells inoculated on special instruments by digitally setting various fluid parameters. However, most commercial fluid shear force loading devices require a computer terminal to set the fluid parameters, and due to the size of the device, they cannot provide cells with a culture environment with a constant temperature and appropriate carbon dioxide concentration. In addition, the cumbersome cell inoculation and the irreplaceable nature of the culture device also make this type of fluid shear force loading device inefficient. Summary of the invention

[0004] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a cell culture device for studying fluid shear force.

[0005] In order to achieve the above object, the present invention is achieved through the following technical solutions:

[0006] A cell culture device for studying fluid shear force comprises a control box, a peristaltic pump, a liquid storage bottle, a damping module, a fluid chamber, and a plurality of glass slides, wherein the peristaltic pump is mounted on the control box, the peristaltic pump is connected to the liquid storage bottle, the liquid storage bottle is connected to the fluid chamber, the fluid chamber is connected to the damping module, the damping module is connected to the peristaltic pump, a plurality of flow slots are provided on the fluid chamber, the glass slide is mounted on the flow slots, and the damping module comprises a plurality of pulse dampers, which are connected in series.

[0007] Preferably, the flow channel is located at the top end of the fluid chamber.

[0008] Preferably, there are two pulse dampers.

[0009] Preferably, there are eight glass slides and eight flow channels.

[0010] Preferably, the peristaltic pump is connected to the liquid storage bottle through a hose, the liquid storage bottle is connected to the fluid chamber through a hose, the fluid chamber is connected to the damping module through a hose, and the damping module is connected to the peristaltic pump through a hose.

[0011] Preferably, the hose is a silicone tube.

[0012] The beneficial effects of the present invention are as follows: the present invention has a small volume and can load fluid shear force in a constant temperature cell culture box, thereby lowering the research threshold of fluid shear force and promoting the development of fluid shear force research. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a three-dimensional structural diagram of the control box;

[0014] Figure 2 is a schematic diagram of the three-dimensional structure of the fluid chamber;

[0015] Figure 3 It is a structural schematic diagram of the present invention;

[0016] Figure 4 This is a schematic diagram of the structure of the damping module. DETAILED DESCRIPTION

[0017] The technical solution of the present invention is further described below in conjunction with the accompanying drawings:

[0018] like Figures 1 to 4 As shown, a cell culture device for studying fluid shear force includes a control box 1, a peristaltic pump 2, a liquid storage bottle 6, a damping module 7, a fluid chamber 3, and a plurality of glass slides 4. The peristaltic pump 2 is installed on the control box 1, the peristaltic pump 2 is connected to the liquid storage bottle 6, the liquid storage bottle 6 is connected to the fluid chamber 3, the fluid chamber 3 is connected to the damping module 7, the damping module 7 is connected to the peristaltic pump 2, the fluid chamber 3 is provided with a plurality of flow slots 5, the glass slide 4 is installed on the flow slots 5, the damping module 7 includes a plurality of pulse dampers 71, and the plurality of pulse dampers 71 are connected in series. The liquid storage bottle 6 is used to hold culture fluid.

[0019] like Figure 2 As shown, the flow channel 5 is located at the top of the fluid chamber 3.

[0020] like Figure 4As shown, there are two pulse dampers 71 .

[0021] like Figure 2 As shown, there are eight glass slides 4 and eight flow channels 5.

[0022] like Figure 3 As shown, the peristaltic pump 2 is connected to the liquid storage bottle 6 through the hose 8, the liquid storage bottle 6 is connected to the fluid chamber 3 through the hose 8, the fluid chamber 3 is connected to the damping module 7 through the hose 8, and the damping module 7 is connected to the peristaltic pump 2 through the hose 8. The hose 8 is a silicone tube.

[0023] like Figures 1 to 4 As shown, culture cells on a glass slide and place it in a fluid chamber. Put culture fluid in a liquid storage bottle. Put silicone tubes on the two glass tubes on the liquid storage bottle. Insert the long-mouthed silicone tube into the peristaltic pump head and connect it to a pulse damper. Then connect the silicone tube at the port to the upper interface of the fluid chamber. Then take a silicone tube to connect the lower interface of the fluid chamber to another pulse damper. Finally, use the silicone tube to connect the two pulse dampers in series. Operate the control box to fill the fluid chamber with culture fluid.

[0024] The culture fluid in the liquid storage bottle is transported to the fluid chamber through the peristaltic pump head, passes through the pulse dampener, and enters the fluid chamber. The fluid chamber is equipped with a glass slide. Cells are cultured on the glass slide. The cells are affected by the shear force of the culture fluid in the flow channel on one side of the glass slide.

[0025] The invention has a small volume and can load fluid shear force in a constant temperature cell culture box, thereby lowering the research threshold of fluid shear force and being conducive to better promoting the development of fluid shear force research.

[0026] It should be noted that the above is only a specific embodiment of the present invention. Obviously, the present invention is not limited to the above embodiment, and there are many variations. In short, all variations that can be directly derived or associated with the content disclosed by ordinary technicians in this field should be considered as the protection scope of the present invention.

Claims

1. A cell culture device for studying fluid shear force, characterized in that: The invention comprises a control box (1), a peristaltic pump (2), a liquid storage bottle (6), a damping module (7), a fluid chamber (3), and a plurality of glass slides (4); the peristaltic pump (2) is mounted on the control box (1); the peristaltic pump (2) is connected to the liquid storage bottle (6); the liquid storage bottle (6) is connected to the fluid chamber (3); the fluid chamber (3) is connected to the damping module (7); the damping module (7) is connected to the peristaltic pump (2); a plurality of flow grooves (5) are arranged on the fluid chamber (3); the glass slide (4) is mounted on the flow grooves (5); the damping module (7) comprises a plurality of pulse dampers (71); and the plurality of pulse dampers (71) are connected in series.

2. A cell culture device for studying fluid shear force according to claim 1, characterized in that: The flow channel (5) is located at the top of the fluid chamber (3).

3. A cell culture device for studying fluid shear force according to claim 1, characterized in that: There are two pulse dampers (71).

4. A cell culture device for studying fluid shear force according to claim 1, characterized in that: There are eight glass slides (4) and eight flow channels (5).

5. A cell culture device for studying fluid shear force according to claim 1, characterized in that: The peristaltic pump (2) is connected to the liquid storage bottle (6) via a hose (8), the liquid storage bottle (6) is connected to the fluid chamber (3) via a hose (8), the fluid chamber (3) is connected to the damping module (7) via a hose (8), and the damping module (7) is connected to the peristaltic pump (2) via a hose (8).

6. A cell culture device for studying fluid shear force according to claim 5, characterized in that: The hose (8) is a silicone hose.

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