Automatic fluid infusion device for cell in-vitro culture

Abstract

The utility model belongs to the technical field of cell experiment equipment, and discloses an automatic liquid supplementing device for cell in-vitro culture, which comprises a bottom plate, a supporting seat arranged on the top surface of the bottom plate, a liquid storage cylinder arranged on the top surface of the supporting seat, a liquid supplementing component arranged on the surface of the liquid storage cylinder and used for conveying culture liquid, and placing grooves arranged on the top surface of the bottom plate in an array manner, a placing groove is formed in the bottom plate, a culture dish is arranged in the placing groove, adjusting assemblies used for positioning the liquid level in the culture dish are arranged on the top surface of the bottom plate in an array mode, drainage assemblies used for distributing culture solutions are arranged in the adjusting assemblies, the adjusting assemblies can rotate on the surface of the liquid storage cylinder, and then different kinds of culture solutions in the liquid storage cylinder are conveyed into different culture dishes. The adjusting assembly can be used for adjusting and positioning according to the size of the culture dish and the depth of a culture solution in the culture dish, so that liquid can be conveniently supplemented to the culture dishes under different conditions in the later stage, and the drainage assembly can be used for buffering the supplemented liquid to prevent the culture solution from quickly entering the culture dish to influence the distribution of cells.

Description

Technical Field

[0001] The utility model relates to the technical field of cell experiment equipment, in particular to an automatic liquid replenishing device for in vitro cell culture. Background Art

[0002] Cell culture technology is also called cell cloning technology. A large number of cells or their metabolites are obtained through cell culture. Because biological products are all obtained from cells, it can be said that cell culture technology is the most core and basic technology in biotechnology. Cell culture work has been widely used in various fields such as biology, medicine, and new drug research and development, and has become one of the most important basic sciences. Cell growth requires a nutritional environment. The nutrient matrix used to maintain cell growth is called a culture medium. The culture medium is divided into culture fluid and culture solid. Among them, in the process of culturing cells with culture fluid, the cells will consume the culture fluid, and it is necessary to replenish the fluid regularly.

[0003] For example, the utility model with announcement number CN209685804U discloses a cell culture rehydration device, including a rehydration bottle, a multi-way connector, a main pipe and a three-way valve. There are at least two rehydration bottles, and the number of ports of the multi-way connector is one more than the number of rehydration bottles. Each rehydration bottle is connected to one of the ports of the multi-way connector, one end of the main pipe is connected to one of the ports of the multi-way connector, and the other end of the main pipe is connected to the three-way valve; the bottle cap of the rehydration bottle is provided with an air inlet pipe and a liquid outlet pipe, the air outlet end of the air inlet pipe is located at the top of the rehydration bottle, the inlet end of the liquid outlet pipe is located at the bottom of the rehydration bottle, and the outlet end of the liquid outlet pipe is connected to the multi-way connector through a branch pipe. When rehydration is needed, gas is injected into the rehydration bottle through the air inlet pipe, and the gas pushes the liquid in the rehydration bottle to flow out through the branch pipe; when drainage is needed, the three-way valve is turned to close the main pipe port. The utility model has a simple structure and is easy to operate. It can replenish multiple liquids at the same time, and it is convenient to drain liquid, which can improve work efficiency.

[0004] In the process of implementing this application, it was found that the technology has the following problems: the device cannot replenish fluids for different cell cultures at the same time, which reduces the functionality of the device, and when the existing fluid replenishment device is replenishing fluid, the liquid enters the culture dish and easily disperses the cells, affecting the distribution of cells and reducing the quality of in vitro cell culture.

[0005] Therefore, an automatic fluid replenishment device for in vitro cell culture is proposed. Utility Model Content

[0006] The purpose of the utility model is to solve the problem that the device cannot perform fluid replenishment work on different cell cultures at the same time, which reduces the functionality of the device, and the existing fluid replenishment device easily disperses cells when liquid enters the culture dish during fluid replenishment, affects the distribution of cells, and reduces the quality of in vitro cell culture. The utility model provides an automatic fluid replenishment device for in vitro cell culture.

[0007] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0008] An automatic fluid replenishment device for in vitro cell culture comprises a base plate, a support seat is provided on the top surface of the base plate, a liquid storage cylinder is provided on the top surface of the support seat, a fluid replenishment component for conveying culture fluid is provided on the surface of the liquid storage cylinder, and the fluid replenishment component is movably connected to the liquid storage cylinder, a placement groove is provided in an array on the top surface of the base plate, a culture dish is provided in the placement groove, an adjustment component for positioning the liquid level in the culture dish is provided in the top surface array of the base plate, and a drainage component for distributing the culture fluid is provided in the adjustment component.

[0009] Furthermore, a partition is provided in the liquid storage cylinder, and a surface array of liquid replenishment holes is opened in the liquid storage cylinder, and the liquid replenishment holes respectively penetrate the sides of the liquid storage cylinder. The liquid replenishment assembly includes a sealing sleeve, and the sealing sleeve is rotatably connected to the liquid storage cylinder, and the sealing sleeve is made of rubber thermoplastic.

[0010] Furthermore, a gear bar is provided on the surface of the sealing sleeve, an adjusting motor is provided in the support seat, an adjusting gear is provided at the output end of the adjusting motor, and the adjusting gear and the gear bar are meshed with each other, a fluid infusion tube is provided in an array on the surface of the sealing sleeve, and the fluid infusion tube passes through the sealing sleeve, and a fluid infusion solenoid valve is provided on the surface of the fluid infusion tube.

[0011] Furthermore, the adjustment component includes a support plate, a lifting groove is opened on the surface of the support plate, a lifting screw and a lifting plate are provided in the lifting groove, and the lifting screw and the lifting plate are respectively movably connected to the lifting groove, and the lifting screw is threadedly connected to the lifting plate, and a lifting motor is fixed to the top of the lifting screw.

[0012] Furthermore, a pressure sensor is provided on the bottom surface of the lifting plate, a connecting rod is provided on the bottom surface of the pressure sensor, and a floating ball is provided at the bottom end of the connecting rod.

[0013] Furthermore, the drainage component includes an electric telescopic rod, and the electric telescopic rod passes through the lifting plate. The output end of the electric telescopic rod is provided with a conical plate, and the top surface array of the conical plate is provided with drainage strips.

[0014] Furthermore, a controller is provided on the top surface of the base plate, and the controller is electrically connected to the regulating motor, the liquid replenishing solenoid valve, the pressure sensor, the lifting motor, and the electric telescopic rod respectively.

[0015] The beneficial effects of the utility model are as follows:

[0016] The utility model provides a liquid replenishing component on the surface of the liquid storage cylinder, provides an adjusting component around the liquid storage cylinder, and provides a drainage component inside the adjusting component. The adjusting component can rotate on the surface of the liquid storage cylinder, and then transport different types of culture fluids in the liquid storage cylinder to different culture dishes. The adjusting component can be adjusted and positioned according to the size of the culture dish and the depth of the culture fluid in the culture dish, so as to facilitate the later liquid replenishment of culture dishes in different situations. The drainage component can buffer the replenished liquid to prevent the culture fluid from quickly entering the culture dish and affecting the distribution of cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is an axonometric drawing of the utility model;

[0018] Figure 2 It is an overall cross-sectional view of the utility model;

[0019] Figure 3 It is an exploded view of the overall structure of the utility model;

[0020] Figure 4 It is a structural diagram of the fluid replenishment component of the utility model;

[0021] Figure 5 It is a cross-sectional view of the adjustment assembly of the utility model;

[0022] Figure 6 It is a structural diagram of the drainage component of the utility model.

[0023] Reference numerals: 1, liquid replenishing assembly; 101, regulating motor; 102, regulating gear; 103, gear bar;

[0024] 104, liquid infusion tube; 105, liquid infusion solenoid valve; 106, sealing sleeve; 2, adjustment component; 201, lifting plate;

[0025] 202, pressure sensor; 203, connecting rod; 204, floating ball; 205, lifting motor; 206, lifting screw;

[0026] 207, lifting slot; 208, support plate; 3, drainage assembly; 301, electric telescopic rod; 302, conical plate; 303, drainage strip; 4, controller; 5, bottom plate; 6, support seat; 7, liquid storage cylinder; 8, partition; 9, liquid replenishment hole; 10, culture dish; 11, placement slot. DETAILED DESCRIPTION

[0027] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0028] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0030] In the description of the embodiments of the present invention, it should be noted that the terms "inside", "outside", "upper", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0031] like Figures 1 to 6 As shown, it includes a bottom plate 5, a support seat 6 is provided on the top surface of the bottom plate 5, a liquid storage cylinder 7 is provided on the top surface of the support seat 6, a liquid infusion component 1 for conveying culture fluid is provided on the surface of the liquid storage cylinder 7, and the liquid infusion component 1 is movably connected to the liquid storage cylinder 7, a placement groove 11 is provided in an array on the top surface of the bottom plate 5, a culture dish 10 is provided in the placement groove 11, an adjustment component 2 for positioning the liquid level in the culture dish 10 is provided in the top surface array of the bottom plate 5, and a drainage component 3 for distributing the culture fluid is provided in the adjustment component 2.

[0032] Specifically, the regulating component 2 can rotate on the surface of the liquid storage cylinder 7, and then transport different types of culture fluid in the liquid storage cylinder 7 to different culture dishes 10, and the regulating component 2 can be adjusted and positioned according to the size of the culture dish 10 and the depth of the culture fluid in the culture dish 10, so as to facilitate the later replenishment of culture dishes 10 in different situations, and the drainage component 3 can buffer the replenished liquid to prevent the culture fluid from quickly entering the culture dish 10 and affecting the distribution of cells.

[0033] A partition 8 is provided in the liquid storage cylinder 7, and a surface array of liquid replenishing holes 9 are opened in the liquid storage cylinder 7, and the liquid replenishing holes 9 respectively penetrate the sides of the liquid storage cylinder 7. The liquid replenishing component 1 includes a sealing sleeve 106, and the sealing sleeve 106 is rotatably connected to the liquid storage cylinder 7. The sealing sleeve 106 is made of rubber thermoplastic, and a gear bar 103 is provided on the surface of the sealing sleeve 106. An adjusting motor 101 is provided in the support seat 6, and an adjusting gear 102 is provided at the output end of the adjusting motor 101, and the adjusting gear 102 and the gear bar 103 are meshed with each other. A liquid replenishing tube 104 is provided in the surface array of the sealing sleeve 106, and the liquid replenishing tube 104 penetrates the sealing sleeve 106, and a liquid replenishing solenoid valve 105 is provided on the surface of the liquid replenishing tube 104.

[0034] Specifically, in the fluid infusion component 1, under the drive of the adjusting motor 101 in the support seat 6, the adjusting gear 102 can drive the sealing sleeve 106 to rotate on the surface of the fluid storage cylinder 7, and then the fluid infusion tube 104 on the surface of the sealing sleeve 106 is aligned with the fluid infusion hole 9 on the surface of the fluid storage tube, and then under the control of the fluid infusion solenoid valve 105, the culture fluid in the fluid storage cylinder 7 will flow out from the fluid infusion tube 104.

[0035] The adjustment component 2 includes a support plate 208, a lifting groove 207 is opened on the surface of the support plate 208, a lifting screw 206 and a lifting plate 201 are arranged in the lifting groove 207, and the lifting screw 206 and the lifting plate 201 are respectively movably connected to the lifting groove 207, and the lifting screw 206 is threadedly connected to the lifting plate 201, a lifting motor 205 is fixed to the top of the lifting screw 206, a pressure sensor 202 is arranged on the bottom surface of the lifting plate 201, a connecting rod 203 is arranged on the bottom surface of the pressure sensor 202, and a floating ball 204 is arranged at the bottom end of the connecting rod 203.

[0036] Specifically, in the adjustment component 2, under the action of the lifting motor 205, the lifting screw 206 drives the lifting plate 201 to move up and down in the lifting groove 207. According to the height of the culture liquid level in the culture dish 10, the float 204 at the bottom of the connecting rod 203 will contact the culture liquid surface, and then the pressure sensor 202 at the top of the connecting rod 203 will monitor the buoyancy of the float 204 at this time. When the liquid in the culture dish 10 drops, the force exerted on the pressure sensor 202 will change, and the liquid replenishment component 1 will start.

[0037] The drainage assembly 3 includes an electric telescopic rod 301, and the electric telescopic rod 301 passes through the lifting plate 201. A conical plate 302 is provided at the output end of the electric telescopic rod 301, and a drainage strip 303 is provided in an array on the top surface of the conical plate 302. A controller 4 is provided on the top surface of the bottom plate 5, and the controller 4 is electrically connected to the regulating motor 101, the fluid infusion solenoid valve 105, the pressure sensor 202, the lifting motor 205, and the electric telescopic rod 301 respectively.

[0038] Specifically, when the culture fluid falls onto the conical plate 302 from the infusion tube 104 , the culture fluid will flow on the top surface of the conical plate 302 and flow into the inner wall of the culture dish 10 along the drainage strip 303 to prevent the cultured cells from being dispersed.

[0039] In summary: in the rehydration component 1, under the drive of the adjusting motor 101 in the support seat 6, the adjusting gear 102 can drive the sealing sleeve 106 to rotate on the surface of the liquid storage tube 7, and then the rehydration tube 104 on the surface of the sealing sleeve 106 is aligned with the rehydration hole 9 on the surface of the liquid storage tube, and then under the control of the rehydration solenoid valve 105, the culture fluid in the liquid storage tube 7 will flow out from the rehydration tube 104. In the adjustment component 2, under the action of the lifting motor 205, the lifting screw rod 206 drives the lifting plate 201 to move up and down in the lifting groove 207. According to the height of the culture fluid level in the culture dish 10, the float 204 at the bottom of the connecting rod 203 will contact the culture fluid surface, and then the pressure sensor 202 at the top of the connecting rod 203 will monitor the buoyancy of the float 204 at this time. When the liquid in the fluid drops, the force exerted on the pressure sensor 202 will change, and the fluid replenishment component 1 will be started at this time. When the culture fluid falls from the fluid replenishment tube 104 onto the conical plate 302, the culture fluid will flow on the top surface of the conical plate 302 and flow in from the inner wall of the culture dish 10 along with the drainage strip 303 to prevent the cultured cells from being dispersed. The adjustment component 2 can rotate on the surface of the fluid storage cylinder 7, and then transport different types of culture fluid in the fluid storage cylinder 7 to different culture dishes 10. The adjustment component 2 can be adjusted and positioned according to the size of the culture dish 10 and the depth of the culture fluid in the culture dish 10, so as to facilitate the later fluid replenishment of the culture dishes 10 in different situations, and the drainage component 3 can buffer the supplemented liquid to prevent the culture fluid from quickly entering the culture dish 10 and affecting the distribution of cells.

[0040] The above shows and describes the basic principle, main features and advantages of the utility model. Those skilled in the art should understand that the utility model is not limited by the above embodiments. The above embodiments and the specification only describe the principles of the utility model. The utility model may be subject to various changes and improvements without departing from the spirit and scope of the utility model. These changes and improvements fall within the scope of the utility model to be protected. The scope of protection claimed by the utility model is defined by the attached claims and their equivalents.

Claims

1. An automatic fluid replenishment device for in vitro cell culture, comprising a bottom plate (5), characterized in that: The top surface of the bottom plate (5) is provided with a support seat (6), the top surface of the support seat (6) is provided with a liquid storage cylinder (7), the surface of the liquid storage cylinder (7) is provided with a liquid replenishing component (1) for conveying culture fluid, and the liquid replenishing component (1) is movably connected to the liquid storage cylinder (7), the top surface array of the bottom plate (5) is provided with a placement groove (11), the placement groove (11) is provided with a culture dish (10), the top surface array of the bottom plate (5) is provided with an adjustment component (2) for positioning the liquid level in the culture dish (10), and the adjustment component (2) is provided with a drainage component (3) for distributing the culture fluid.

2. The automatic fluid replenishment device for in vitro cell culture according to claim 1, characterized in that: The liquid storage cylinder (7) is provided with a partition (8), and a surface array of liquid replenishing holes (9) is provided on the liquid storage cylinder (7), and the liquid replenishing holes (9) respectively penetrate the side surfaces of the liquid storage cylinder (7). The liquid replenishing assembly (1) comprises a sealing sleeve (106), and the sealing sleeve (106) is rotatably connected to the liquid storage cylinder (7), and the sealing sleeve (106) is made of rubber thermoplastic.

3. The automatic fluid replenishment device for in vitro cell culture according to claim 2, characterized in that: The surface of the sealing sleeve (106) is provided with a gear bar (103), the support seat (6) is provided with an adjusting motor (101), the output end of the adjusting motor (101) is provided with an adjusting gear (102), and the adjusting gear (102) and the gear bar (103) are meshed with each other, the surface array of the sealing sleeve (106) is provided with a liquid infusion tube (104), and the liquid infusion tube (104) passes through the sealing sleeve (106), and the surface of the liquid infusion tube (104) is provided with a liquid infusion solenoid valve (105).

4. The automatic fluid replenishment device for in vitro cell culture according to claim 3, characterized in that: The adjustment component (2) comprises a support plate (208), a lifting groove (207) is provided on the surface of the support plate (208), a lifting screw rod (206) and a lifting plate (201) are provided in the lifting groove (207), the lifting screw rod (206) and the lifting plate (201) are movably connected to the lifting groove (207) respectively, and the lifting screw rod (206) is threadedly connected to the lifting plate (201), and a lifting motor (205) is fixed at the top end of the lifting screw rod (206).

5. The automatic fluid replenishment device for in vitro cell culture according to claim 4, characterized in that: The bottom surface of the lifting plate (201) is provided with a pressure sensor (202), the bottom surface of the pressure sensor (202) is provided with a connecting rod (203), and the bottom end of the connecting rod (203) is provided with a floating ball (204).

6. The automatic fluid replenishment device for in vitro cell culture according to claim 5, characterized in that: The drainage assembly (3) comprises an electric telescopic rod (301), and the electric telescopic rod (301) passes through the lifting plate (201), and the output end of the electric telescopic rod (301) is provided with a conical plate (302), and the top surface array of the conical plate (302) is provided with drainage strips (303).

7. The automatic fluid replenishment device for in vitro cell culture according to claim 6, characterized in that: A controller (4) is provided on the top surface of the bottom plate (5), and the controller (4) is electrically connected to the regulating motor (101), the liquid replenishing solenoid valve (105), the pressure sensor (202), the lifting motor (205), and the electric telescopic rod (301) respectively.

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