Device for in-vitro sugar stimulation of islet cells

Abstract

The utility model discloses a device for in-vitro sugar stimulation of islet cells, and relates to the technical field of islet cell experimental devices. The device comprises two backflow simulation pipes, two connecting hoses are arranged between the two backflow simulation pipes, the two backflow simulation pipes are communicated through the two connecting hoses, one backflow simulation pipe is provided with a pipeline width simulation mechanism, one connecting hose is provided with a temperature control peristaltic pump, and the other connecting hose is provided with a temperature control peristaltic pump. And an experiment box is arranged on the other connecting hose. The device can better simulate the influence of the blood vessel blocking state on insulin in the in-vivo flowing process, and is more in line with some in-vivo conditions that blood vessels become narrow due to high blood fat, so that experimental results can be more in line with the real in-vivo conditions, and a certain amount of fat cells are filled in the device, so that the experimental results are more accurate. The in-vitro experiment result generated by the islet cells can better verify the in-vivo working condition of the islet cells.

Description

Technical Field

[0001] The utility model relates to the technical field of islet cell experimental devices, in particular to a device for in vitro sugar stimulation of islet cells. Background Art

[0002] Currently, most of the pancreatic islet cells and culture fluid in the culture dish are in a static state. Usually, the in vitro sugar stimulation experiment of pancreatic islet cells is usually carried out in the culture dish. However, this is somewhat different from the actual state of cells and hormone flow in the body. Therefore, there is a certain difference between the experimental effect and the application of the insulin secretion function of real pancreatic islet cells in the body, so the experimental results cannot verify the actual situation of pancreatic islet cells working in the body. For this reason, a device for in vitro sugar stimulation of pancreatic islet cells is proposed. Utility Model Content

[0003] The purpose of the utility model is to solve the problem that there is a certain difference between the experimental effect and the insulin secretion function of real pancreatic islet cells in vivo, so that the experimental results cannot verify the actual working situation of pancreatic islet cells in vivo. The utility model provides a device for in vitro sugar stimulation of pancreatic islet cells.

[0004] In order to achieve the above-mentioned purpose, the utility model specifically adopts the following technical solutions:

[0005] A device for in vitro sugar stimulation of pancreatic islet cells comprises two reflux simulation tubes, two connecting hoses are arranged between the two reflux simulation tubes, and the two reflux simulation tubes are connected through the two connecting hoses, one of the reflux simulation tubes is provided with a pipeline width simulation mechanism, one of the connecting hoses is provided with a temperature-controlled peristaltic pump, and the other of the connecting hoses is provided with an experimental box, and a simulation stimulation mechanism is provided in the experimental box.

[0006] Preferably, the pipeline width simulation mechanism includes an adjustment tube, and both ends of the adjustment tube are connected to the reflux simulation tube, a soft-gel airbag is arranged in the adjustment tube, and an air inflation tube is fixedly installed on the side wall of the soft-gel airbag, one end of the air inflation tube passes through the adjustment tube and is fixedly installed with an air bag, and an exhaust valve is arranged on the air bag, and a fat simulation layer is arranged on the side wall of the soft-gel airbag.

[0007] Preferably, the reflux simulation tube is arranged in two sections, and one end of the two sections of the reflux simulation tube is provided with a threaded connection notch, and both ends of the adjustment tube are provided with a trapezoidal threaded connector, and the trapezoidal threaded connector is matched with the threaded connection notch.

[0008] Preferably, the upper end of the experiment box is open, and a T-shaped cover is provided in the opening of the experiment box. Positioning bolts are provided on the side walls of the T-shaped cover, and the T-shaped cover is connected to the experiment box via the positioning bolts.

[0009] Preferably, the simulation stimulation mechanism includes a connecting screw tube, a connecting screw hole is provided on the side wall of the T-shaped cover plate, an external thread is provided on the outer side wall of the connecting screw tube, and the connecting screw tube is threadedly installed in the connecting screw hole, a filter cylinder is fixedly installed at one end of the connecting screw tube, and a sealing plug is fixedly installed at the other end of the connecting screw tube, an air supply pipe is provided on the side wall of the T-shaped cover plate on one side of the connecting screw tube, and a liquid exchange pipe is provided on the T-shaped cover plate on one side of the air supply pipe.

[0010] Preferably, a plurality of support columns are fixedly mounted on the side wall of the reflux simulation tube, and the support columns are arranged in a conical structure.

[0011] The beneficial effects of the utility model are as follows:

[0012] 1. In the utility model, by providing components such as a temperature-controlled peristaltic pump and an adjustment tube, air can be injected into the soft-gel airbag via an air inflation tube, thereby expanding the soft-gel airbag. Combined with the fat simulation layer on the surface of the device, the effect of blood vessel blockage on the flow of insulin in the body can be better simulated, which is more in line with some in vivo conditions where blood vessels are narrowed due to high blood lipids. This can make the experimental results more consistent with the actual situation in the body.

[0013] 2. In the utility model, the liquid environment and nutrients in the device can be replaced in time through the liquid exchange tube, and the gas supply pipe can be used to supply a specified proportion of gas source to the device for cell survival. At the same time, a connecting spiral tube and a filter cylinder are added to the experimental box, and a certain amount of fat cells are filled in the filter cylinder, which can better fit the situation in which pancreatic islet cells are affected by fat cells to varying degrees in the body, so that the in vitro experimental results produced by the pancreatic islet cells can better verify their working conditions in the body. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 This is a front view schematic diagram of the overall three-dimensional connection structure of the utility model;

[0015] Figure 2 It is a schematic diagram of the three-dimensional connection structure of the experimental box and the connecting hose in the utility model;

[0016] Figure 3 It is a schematic diagram of the three-dimensional connection structure of the pipeline width simulation mechanism in the utility model;

[0017] Figure 4 It is a schematic diagram of the three-dimensional connection structure of the connecting spiral tube and the filter cylinder in the utility model.

[0018] Figure numerals: 1. Reflux simulation tube; 2. Support column; 3. Adjustment tube; 4. Connecting hose; 5. Temperature-controlled peristaltic pump; 6. Experimental box; 7. T-shaped cover plate; 8. Sealing plug; 9. Connecting screw hole; 10. Connecting screw tube; 11. Positioning bolt; 12. Air supply pipe; 13. Liquid exchange tube; 14. Soft rubber airbag; 15. Fat simulation layer; 16. Air inflation tube; 17. Inflation bag; 18. Exhaust valve; 19. Trapezoidal threaded connector; 20. Filter cylinder. DETAILED DESCRIPTION

[0019] In order to make the purpose, technical scheme and advantages of the embodiments of the utility model clearer, the technical scheme in the embodiments of the utility model will be clearly and completely described below in conjunction with the drawings in the embodiments of the utility model. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Generally, the components of the embodiments of the utility model described and shown in the drawings here can be arranged and designed in various different configurations.

[0020] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the present invention to be protected, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0022] In the description of the embodiments of the present invention, it should be noted that the terms "inside", "outside", "upper", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, or are the orientations or positional relationships in which the utility model product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0023] like Figure 1-4As shown, a device for in vitro glucose stimulation of pancreatic islet cells includes two reflux simulation tubes 1, two connecting hoses 4 are arranged between the two reflux simulation tubes 1, and the two reflux simulation tubes 1 are connected through the two connecting hoses 4, and a plurality of support columns 2 are fixedly installed on the side walls of the reflux simulation tubes 1, and the support columns 2 are arranged in a conical structure. The support columns 2 can better make the reflux simulation tubes 1 and the connecting hoses 4 leave the ground, so that it is convenient for the experimenter to move the entire device.

[0024] A pipeline width simulation mechanism is provided on one of the reflux simulation tubes 1, and the pipeline width simulation mechanism includes an adjustment tube 3. The reflux simulation tube 1 is arranged in two sections, and one end of the two sections of the reflux simulation tube 1 is provided with a threaded connection groove. Both ends of the adjustment tube 3 are provided with a trapezoidal threaded connector 19, and the trapezoidal threaded connector 19 is matched with the threaded connection groove, so that the two ends of the adjustment tube 3 are detachably connected to the reflux simulation tube 1, thereby facilitating the maintenance and replacement of the structure inside the adjustment tube 3.

[0025] A soft-gel airbag 14 is arranged in the adjustment tube 3, and an air inflation tube 16 is fixedly installed on the side wall of the soft-gel airbag 14, one end of the air inflation tube 16 passes through the adjustment tube 3 and is fixedly installed with an air-inflating bag 17, and an exhaust valve 18 is arranged on the air-inflating bag 17, and a fat simulation layer 15 is arranged on the side wall of the soft-gel airbag 14. By using the air-inflating bag 17 and injecting air into the soft-gel airbag 14 with the help of the air inflation tube 16, the soft-gel airbag 14 is expanded, and the fat simulation layer 15 on the surface of the device can better simulate the influence of the blood vessel blockage state on the flow of insulin in the body, so that the experimental results can be more in line with the actual situation in the body.

[0026] A temperature-controlled peristaltic pump 5 is arranged on one of the connecting hoses 4, and an experimental box 6 is arranged on the other connecting hose 4. The upper end of the experimental box 6 is arranged in an open shape, and a T-shaped cover plate 7 is arranged in the opening of the experimental box 6. Positioning bolts 11 are arranged on the side walls of the T-shaped cover plate 7, and the T-shaped cover plate 7 and the experimental box 6 are connected by the positioning bolts 11. By providing a detachable T-shaped cover plate 7, it is convenient to clean the device, thereby facilitating further experiments.

[0027] A simulation stimulation mechanism is provided in the experimental box 6, and the simulation stimulation mechanism includes a connecting screw tube 10. A connecting screw hole 9 is provided on the side wall of the T-shaped cover plate 7, and an external thread is provided on the outer wall of the connecting screw tube 10. The connecting screw tube 10 is threadedly installed in the connecting screw hole 9, and a filter cylinder 20 is fixedly installed at one end of the connecting screw tube 10. The connecting screw tube 10 and the filter cylinder 20 are added in the experimental box 6, and a certain amount of fat cells are filled in the filter cylinder 20, which can better fit the situation in which the pancreatic islet cells are affected by fat cells to different degrees in the body, so that the in vitro experimental results produced by the pancreatic islet cells can better verify their working conditions in the body.

[0028] A sealing plug 8 is fixedly installed on the other end of the connecting spiral tube 10, and an air supply pipe 12 is arranged on the side wall of the T-shaped cover plate 7 on one side of the connecting spiral tube 10. With the help of the arranged air supply pipe 12, a specified proportion of air source can be supplied to the device for cell survival. A liquid exchange tube 13 is arranged on the T-shaped cover plate 7 on one side of the air supply pipe 12. The liquid environment and nutrients in the device can be replaced in time through the arranged liquid exchange tube 13.

[0029] In summary: when conducting the experiment, start the set temperature-controlled peristaltic pump 5, so that the temperature-controlled peristaltic pump 5 simulates the body temperature and makes the liquid in the device simulate the flow in the body, and then use the air bag 17 to inject air into the soft-gel air bag 14 with the help of the air inflation tube 16, so that the soft-gel air bag 14 expands, and cooperates with the fat simulation layer 15 on the surface of the device, which can better simulate the influence of the blood vessel blockage state on the flow of insulin in the body, and then the liquid environment and nutrients in the device are replaced in time through the set liquid exchange tube 13, and the set air supply tube 12 is used to supply the specified proportion of air source to the device for cell survival. At the same time, a connecting spiral tube 10 and a filter cylinder 20 are added to the experimental box 6, and a certain amount of fat cells are filled in the filter cylinder 20, which can better fit the situation that the pancreatic islet cells are affected by fat cells to different degrees in the body.

[0030] The above description enables those skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for in vitro glucose stimulation of pancreatic islet cells, characterized in that: The invention comprises two reflux simulation tubes (1), two connecting hoses (4) are arranged between the two reflux simulation tubes (1), and the two reflux simulation tubes (1) are connected and arranged through the two connecting hoses (4), one of the reflux simulation tubes (1) is provided with a pipeline width simulation mechanism, one of the connecting hoses (4) is provided with a temperature-controlled peristaltic pump (5), and the other of the connecting hoses (4) is provided with an experimental box (6), and a simulation stimulation mechanism is provided in the experimental box (6).

2. The device for in vitro glucose stimulation of pancreatic islet cells according to claim 1, characterized in that: The pipeline width simulation mechanism comprises an adjustment tube (3), and both ends of the adjustment tube (3) are connected to the reflux simulation tube (1), a soft rubber airbag (14) is arranged in the adjustment tube (3), and an air inflation tube (16) is fixedly installed on the side wall of the soft rubber airbag (14), one end of the air inflation tube (16) passes through the adjustment tube (3) and is fixedly installed with an air inflation tube (17), and an exhaust valve (18) is arranged on the air inflation tube (17), and a fat simulation layer (15) is arranged on the side wall of the soft rubber airbag (14).

3. The device for in vitro glucose stimulation of pancreatic islet cells according to claim 2, characterized in that: The reflux simulation tube (1) is arranged in two sections, and one end of each of the two sections of the reflux simulation tube (1) is provided with a threaded connection notch. Both ends of the adjustment tube (3) are provided with a trapezoidal threaded connector (19), and the trapezoidal threaded connector (19) is arranged to match the threaded connection notch.

4. The device for in vitro glucose stimulation of pancreatic islet cells according to claim 1, characterized in that: The upper end of the experimental box (6) is arranged in an open shape, and a T-shaped cover plate (7) is arranged in the opening of the experimental box (6). Positioning bolts (11) are arranged on the side walls of the T-shaped cover plate (7), and the T-shaped cover plate (7) and the experimental box (6) are connected via the positioning bolts (11).

5. The device for in vitro glucose stimulation of pancreatic islet cells according to claim 4, characterized in that: The simulation stimulation mechanism comprises a connecting spiral tube (10), a connecting screw hole (9) is provided on the side wall of the T-shaped cover plate (7), an external thread is provided on the outer side wall of the connecting spiral tube (10), and the connecting spiral tube (10) is threadedly installed in the connecting screw hole (9), a filter screen cylinder (20) is fixedly installed on one end of the connecting spiral tube (10), and a sealing plug (8) is fixedly installed on the other end of the connecting spiral tube (10), an air supply pipe (12) is provided on the side wall of the T-shaped cover plate (7) at one side of the connecting spiral tube (10), and a liquid exchange pipe (13) is provided on the T-shaped cover plate (7) at one side of the air supply pipe (12).

6. The device for in vitro glucose stimulation of pancreatic islet cells according to claim 1, characterized in that: A plurality of support columns (2) are fixedly mounted on the side wall of the reflux simulation tube (1), and the support columns (2) are arranged in a conical structure.

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