Stem cell isolated culture device

Abstract

The utility model provides a stem cell separation culture device, which relates to the technical field of stem cell separation culture, and comprises a culture box and a placing rack, and further comprises a supporting part arranged in the culture box, a limiting part with one end connected with the supporting part and used for dividing the space on the placing rack, and a fixing part arranged on the placing rack and used for fixing the space on the placing rack. And the connecting part is connected with the other end of the limiting part. According to the culture bottle stacking device, the limiting part is moved to the designated position of the placing frame, the placing frame is moved to the designated height position in the culture box according to the height of the culture bottles to be stacked, the height of the limiting part is correspondingly increased, the limiting part is fixed through the fixing part, and the culture bottles are stacked and placed on the placing frame; therefore, the stacked culture bottles can be limited and supported by the limiting part, the probability that the stacked culture bottles are knocked down by workers is reduced, and the stability of the stacked culture bottles in the incubator is improved.

Description

Technical Field

[0001] The utility model relates to the technical field of stem cell separation and culture, in particular to a stem cell separation and culture device. Background Art

[0002] Stem cell separation and culture is a technology that separates stem cells from a mixed cell population and cultures and proliferates them under appropriate culture conditions. The sample is separated by a separation device to achieve stem cell separation of the sample, the separated stem cells are placed in a culture bottle, and the culture bottle is placed in a culture device for culture. Most of the existing technologies use an incubator for culture.

[0003] In the prior art, due to the limited internal space of the incubator, culture bottles containing stem cells are usually stacked and placed in the incubator. Workers are prone to knocking over the stacked culture bottles in the incubator when taking out or placing the culture bottles, which makes it easy for the stem cells in the culture bottles to leak out and affect the stem cell culture work, resulting in low stability of the stacked culture bottles in the incubator.

[0004] In view of this, this application is hereby filed. Utility Model Content

[0005] The utility model aims to provide a stem cell separation and culture device, which can fix and support stacked culture bottles through a supporting part, a limiting part and a fixing part.

[0006] In order to solve the above technical problems, the technical solution of the utility model is as follows:

[0007] The embodiment of the utility model provides a stem cell separation and culture device, comprising a culture box and a placement rack, and further comprising:

[0008] A support portion, disposed in the incubator;

[0009] A limiting part, one end of which is connected to the supporting part and divides the space on the placement rack, and the limiting parts cooperate with each other to clamp and limit the culture bottles stacked on the placement rack;

[0010] The fixing part is arranged on the placing frame and connected with the other end of the limiting part. The fixing part cooperates with the supporting part to support the limiting part.

[0011] Furthermore, the support portion includes:

[0012] A connecting rod, one end of which is fixed on the placement rack;

[0013] The support member is provided with a long slot and is arranged at the other end of the connecting rod.

[0014] Furthermore, the limiting portion includes:

[0015] A mounting member, disposed in the supporting member;

[0016] A limiting support member has one end connected to the mounting member and is arranged on the placement rack.

[0017] Furthermore, the mounting member includes:

[0018] The sliding shell is arranged in the long groove of the support member, one end of the spring is connected to the inner side of the bottom wall of the sliding shell, the other end of the spring is connected to the movable block, and a part of the movable block is located in the sliding shell, and the other part is located in the long groove of the support member.

[0019] Furthermore, the mounting member further comprises:

[0020] A pressing plate, fixed on the movable block;

[0021] A connecting block has one end connected to the sliding shell.

[0022] Furthermore, the position limiting support member includes:

[0023] A fixing plate, having a first mounting groove, one end of which is connected to the other end of the connecting block;

[0024] The extension plate is provided with a second installation groove, is arranged on the first installation groove of the fixing plate, and matches with the fixing plate.

[0025] Furthermore, the fixing portion includes:

[0026] A rubber surface is arranged on the square groove of the placement rack;

[0027] The fixing piece is connected with the other end of the fixing plate, is sleeved on the placing frame, and is in contact with the rubber surface.

[0028] The above solution of the utility model includes at least the following beneficial effects:

[0029] The utility model installs a corresponding number of limiting parts in the supporting part according to the culture bottles to be placed in the incubator, and moves the limiting parts to the specified positions of the placing racks according to the positions and shapes of the culture bottles. According to the height of the culture bottles to be stacked, the placing racks are moved to the specified height position in the incubator, and the height of the limiting parts is lengthened accordingly. The limiting parts are fixed by the fixing parts, and the culture bottles are stacked and placed on the placing racks, so that the limiting parts can provide limiting support for the stacked culture bottles, reducing the probability of workers knocking over the stacked culture bottles, thereby increasing the stability of the stacked culture bottles in the incubator. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 It is a three-dimensional diagram of the stem cell separation and culture device of the present utility model;

[0031] Figure 2 A three-dimensional diagram of a placement rack of the stem cell separation and culture device of the present invention;

[0032] Figure 3 A three-dimensional diagram of a fixed plate of the stem cell separation and culture device of the present invention;

[0033] Figure 4 This is an exploded perspective view of the extended plate of the stem cell separation and culture device of the utility model;

[0034] Figure 5 A three-dimensional diagram of the sliding shell of the stem cell separation and culture device of the present invention;

[0035] Figure 6 It is an exploded perspective view of the sliding shell of the stem cell separation and culture device of the utility model;

[0036] Description of reference numerals:

[0037] 1. Incubator; 101. Inner frame; 102. Controller; 2. Box door; 201. Handle; 202. Observation mirror; 3. Placement rack; 301. Rubber surface; 302. Connecting rod; 303. Guide shell; 4. Sliding shell; 401. Spring; 402. Movable block; 403. Pressing plate; 404. Connecting block; 5. Fixed plate; 501. Extension plate; 502. Limiting plate; 503. L-shaped frame plate; 504. Extrusion bolt. DETAILED DESCRIPTION

[0038] The exemplary embodiments of the present invention will be described in more detail below with reference to the accompanying drawings. Although the exemplary embodiments of the present invention are shown in the accompanying drawings, it should be understood that the present invention can be implemented in various forms and should not be limited by the embodiments described herein. On the contrary, these embodiments are provided to enable a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0039] like Figures 1 to 6 As shown, an embodiment of the utility model provides a stem cell separation and culture device, comprising: a culture box 1 and a placement rack 3, and also comprising:

[0040] A support portion, arranged in the incubator 1;

[0041] A limiting part, one end of which is connected to the supporting part and divides the space on the placement rack 3, and the limiting parts cooperate with each other to clamp and limit the culture bottles stacked on the placement rack 3;

[0042] A fixing part, which is arranged on the placement frame 3 and connected to the other end of the limiting part, and the fixing part and the supporting part cooperate with each other to support the limiting part;

[0043] Specifically, three groups of inner frames 101 are symmetrically installed on the inner wall of the incubator 1, and two inner frames 101 form a group. A controller 102 is installed on one side of the incubator 1, and a door 2 is installed on the other side of the incubator 1 through two hinges. An opening is opened through the inside of the door 2, a handle 201 is installed on the front of the door 2, and an observation mirror 202 is installed on the inner side of the opening. A placement rack 3 is placed on the inner side of the bottom wall of the incubator 1 and on the top of the inner frame 101 in the middle of the incubator 1, and a square groove is opened on the front of the placement rack 3.

[0044] In the embodiment of the utility model, the incubator 1 can support the components associated with it, and the incubator 1 can protect the stem cells in the culture bottles placed inside the incubator 1. The temperature device in the incubator 1 can be controlled by the controller 102 to adjust the temperature in the incubator 1 so that the stem cells in the culture bottles can be cultured. The inner frame 101 can provide placement support for the placement rack 3 under the fixed support of the incubator 1. The door 2 can cooperate with the observation mirror 202 to control the opening and closing of the front of the incubator 1. The door 2 is opened to facilitate the staff to take the culture bottles, and the door 2 is closed to seal the front of the incubator 1. The observation mirror 202 is made of transparent anti-fog glass material, so that the staff can observe the culture work in the incubator 1 at any time.

[0045] The staff adjusts the temperature in the incubator 1 through the controller 102 according to the temperature suitable for the stem cells to be cultured. When the temperature in the incubator 1 reaches the temperature for culturing stem cells, the staff opens the switch of the door 2 through the key and pulls the handle 201 so that the handle 201 can drive the door 2 to move under the action of external force, so that the door 2 can rotate horizontally around the hinge under the action of external force, so that the staff can open the door 2. According to the number of culture bottles to be placed in the incubator 1 and the space occupied by the culture bottles themselves, a suitable number of placement racks 3 are placed on the inner rack 101 at the designated position or on the inner side of the bottom wall of the incubator 1, and the culture bottles are placed on the top of the placement racks 3. A large number of culture bottles need to be stacked on the top of the placement racks 3. The staff drives the door 2 to close the incubator 1 through the handle 201 and locks the door 2 with the key to carry out the culture work of stem cell separation.

[0046] Figures 1 to 6 As shown, the support portion includes:

[0047] A connecting rod 302, one end of which is fixed on the placement rack 3;

[0048] A support member having a long slot and disposed at the other end of the connecting rod 302;

[0049] Specifically, one end of a group of connecting rods 302 is installed on the top of the placement rack 3, and the support member is a guide shell 303. The other end of the group of connecting rods 302 is installed with a guide shell 303, and a long groove is opened on one side of the guide shell 303.

[0050] In an embodiment of the utility model, a group of connecting rods 302 can provide fixed support for the guide shell 303 under the support of the placement rack 3. The guide shell 303 can provide guidance for the movement of the limiting part under the action of external force through the long groove, and can provide stability for the movement of the limiting part under the action of external force.

[0051] Figures 1 to 6 As shown, the limiting portion includes:

[0052] A mounting member, disposed in the supporting member;

[0053] A limiting support member, one end of which is connected to the mounting member and is disposed on the placement frame 3;

[0054] Figures 1 to 6 As shown, the mounting member comprises:

[0055] The sliding shell 4 is arranged in the long groove of the support member, one end of the spring 401 is connected to the inner side of the bottom wall of the sliding shell 4, the other end of the spring 401 is connected to the movable block 402, and a part of the movable block 402 is located in the sliding shell 4, and the other part thereof is located in the long groove of the support member;

[0056] A pressing plate 403, fixed on the movable block 402;

[0057] A connecting block 404, one end of which is connected to the sliding housing 4;

[0058] Figures 1 to 6 As shown, the limiting support member includes:

[0059] The fixing plate 5 is provided with a first mounting groove, one end of which is connected to the other end of the connecting block 404;

[0060] The extension plate 501 is provided with a second mounting groove, which is arranged on the first mounting groove of the fixing plate 5 and matches with the fixing plate 5;

[0061] Specifically, two sliding shells 4 are installed on the inner side of the long groove of the guide shell 303, one end of the spring 401 is connected to the inner side of the bottom wall of the sliding shell 4, the other end of the spring 401 is connected to the movable block 402, and a part of the movable block 402 is located on the inner side of the sliding shell 4, and the other part of the movable block 402 is located on the inner side of the long groove of the guide shell 303, a pressing plate 403 is installed on one side of the movable block 402, and the pressing plate 403 is located on one side of the guide shell 303, a connecting block 404 is installed on one side of the sliding shell 4, and the connecting block 404 is located at the bottom of the pressing plate 403, a fixing plate 5 is installed on one side of the connecting block 404, and the fixing plate 5 is located at the top of the placement rack 3, a first mounting groove is opened on the top of the fixing plate 5, the bottom of the extension plate 501 is embedded and installed on the inner side of the first mounting groove, and a second mounting groove is opened on the top of the extension plate 501.

[0062] In the embodiment of the utility model, the sliding shell 4 can provide support for the spring 401, and can provide activity space for the spring 401 and the movable block 402. The connecting block 404 can provide fixed support for the fixed plate 5 under the support of the sliding shell 4. The fixed plate 5 can provide support for the extension plate 501 through the first mounting groove. The fixed plate 5 can divide the top space of the placement rack 3. The extension plate 501 can cooperate with the fixed plate 5 to divide the top space of the placement rack 3 under the support of the fixed plate 5. At the same time, the fixed plate 5 and the extension plate 501 can provide limited support for the stacked culture bottles, and the stability of the stacked culture bottles can be increased by clamping the culture bottles in a limited manner.

[0063] The staff presses the pressure plate 403, so that the pressure plate 403 can drive the movable block 402 to move under the action of external force, so that the movable block 402 moves along the inner side of the sliding shell 4 under the action of external force, so that the staff can move the sliding shell 4 and the movable block 402 into the inner side of the long groove of the guide shell 303, and then the staff releases the pressure plate 403, so that the pressure plate 403 no longer applies external force to the movable block 402, so that the spring 401 can use its own elastic force to drive the movable block 402 to move upward along the inner side of the sliding shell 4, so that the movable block 402 cooperates with the slider to be clamped into the inner side of the long groove of the guide shell 303. According to the culture bottles to be placed on the placement rack 3, a corresponding number of sliding shells 4 are installed, so that a corresponding number of fixed plates 5 are set on the placement rack 3. According to the culture bottles to be stacked, it is determined whether the extension plate 501 needs to be installed. When the extension plate 501 needs to be installed, the bottom of the extension plate 501 is slidably inserted into the first installation groove of the fixed plate 5 to install the extension plate 501. According to the height of the stacked culture bottles, a specified number of extension plates 501 are installed so that the extension plates 501 cooperate with the fixed plate 5 to limit the support of the stacked culture bottles, thereby increasing the stability of the stacked culture bottles.

[0064] Figures 1 to 6 As shown, the fixing portion includes:

[0065] The rubber surface 301 is arranged on the square groove of the placement rack 3;

[0066] A fixing member connected to the other end of the fixing plate 5, sleeved on the placement frame 3, and in contact with the rubber surface 301;

[0067] Specifically, the fixing part is composed of a limiting plate 502, an L-shaped frame plate 503 and an extrusion bolt 504. The limiting plate 502 is installed on one side of the other end of the fixing plate 5, and the L-shaped frame plate 503 is installed on one side of the limiting plate 502. A threaded hole is penetrated inside the L-shaped frame plate 503, and an extrusion bolt 504 is threadedly installed on the inner side of the threaded hole of the L-shaped frame plate 503, and the extrusion bolt 504 is in contact with the rubber surface 301.

[0068] In the embodiment of the utility model, the limiting plate 502 can provide fixed support for the L-shaped frame plate 503 under the support of the fixed plate 5, the rubber surface 301 itself has toughness and strong friction with external objects, and the extrusion bolt 504 abuts against the rubber surface 301 under the thread support of the L-shaped frame plate 503, so that the friction between the extrusion bolt 504 and the rubber surface 301 can fix and support the fixed plate 5 through the L-shaped frame plate 503 and the limiting plate 502;

[0069] Under the action of external force, the fixed plate 5 can drive the L-shaped frame plate 503 to move through the limit plate 502, so that the limit plate 502 and the L-shaped frame plate 503 cooperate with each other to move along the front side of the placement rack 3. When the fixed plate 5 moves to the specified position, the staff twists the squeezing bolt 504 to move the squeezing bolt 504 under the threaded support of the L-shaped frame plate 503, so that the squeezing bolt 504 squeezes the rubber surface 301, and uses the friction force with the rubber surface 301 to fix the fixed plate 5.

[0070] Working principle: the staff turns on the switch of the box door 2 by using the key, opens the box door 2 by pulling the handle 201, moves the placement rack 3 to a group of inner racks 101 at the designated position and the inner side of the bottom wall of the incubator 1 according to the space in the incubator 1 to be occupied by the culture bottle, and moves a designated number of placement racks 3 into the inner side of the incubator 1 according to the culture bottles to be placed, presses the pressure plate 403 downward, so that the pressure plate 403 drives the movable block 402 to move downward along the inner side of the sliding shell 4, moves a designated number of sliding shells 4 and movable blocks 402 into the inner side of the long groove of the guide shell 303, and then releases the pressure plate 403, so that the spring 401 uses its own elastic force to drive the movable block 402 to move to the initial position along the inner side of the sliding shell 4, so that the movable block 402 cooperates with the sliding shell 4 to be clamped on the inner side of the long groove of the guide shell 303, and the culture bottles to be placed on the placement rack 3 are placed. The height of the culture bottles stacked on the rack 3 is determined by installing a specified number of extension plates 501 on the first installation groove of the fixed plate 5, and installing the subsequently added extension plates 501 on the second installation groove of the extension plates 501 spliced ​​with the fixed plate 5, closing the door 2, locking the door 2, adjusting the temperature in the incubator 1 through the controller 102, and when the temperature reaches the temperature for culturing stem cells, opening the door 2 again, stacking the stem cell culture bottles to be cultured on the top of the placement rack 3 and between the specified number of fixed plates 5 and the extension plates 501, moving the fixed plate 5 so that the fixed plate 5 drives the extension plates 501 to limit and clamp the stacked culture bottles, twisting the squeezing bolt 504 so that the squeezing bolt 504 squeezes the rubber surface 301 to fix the fixed plate 5 through the L-shaped frame plate 503 and the limiting plate 502, and then closing the door 2.

[0071] The above is a preferred embodiment of the present invention. It should be pointed out that, for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A stem cell separation and culture device, comprising a culture box (1) and a placement rack (3), characterized in that: Also includes: A support portion, arranged in the incubator (1); A limiting part, one end of which is connected to the supporting part and divides the space on the placement rack (3), and the limiting parts cooperate with each other to clamp and limit the culture bottles stacked on the placement rack (3); The fixing part is arranged on the placement frame (3) and is connected to the other end of the limiting part. The fixing part and the supporting part cooperate with each other to realize supporting the limiting part.

2. The stem cell separation and culture device according to claim 1, characterized in that: The support portion comprises: A connecting rod (302), one end of which is fixed on the placement rack (3); The support member is provided with a long slot and is arranged at the other end of the connecting rod (302).

3. The stem cell separation and culture device according to claim 2, characterized in that: The limiting part comprises: A mounting member, disposed in the supporting member; A limiting support member has one end connected to the mounting member and is arranged on the placement frame (3).

4. The stem cell separation and culture device according to claim 3, characterized in that: The mounting member comprises: The sliding shell (4) is arranged in the long groove of the supporting member, one end of a spring (401) is connected to the inner side of the bottom wall of the sliding shell (4), the other end of the spring (401) is connected to a movable block (402), and a part of the movable block (402) is located in the sliding shell (4), and the other part is located in the long groove of the supporting member.

5. The stem cell separation and culture device according to claim 4, characterized in that: The mounting member further comprises: A pressing plate (403) fixed on the movable block (402); A connecting block (404) has one end connected to the sliding housing (4).

6. The stem cell separation and culture device according to claim 5, characterized in that: The position limiting support member comprises: A fixing plate (5) having a first mounting groove and one end of which is connected to the other end of the connecting block (404); The extension plate (501) is provided with a second installation groove, is arranged on the first installation groove of the fixing plate (5), and matches with the fixing plate (5).

7. The stem cell separation and culture device according to claim 6, characterized in that: The fixing portion comprises: A rubber surface (301) is arranged on the square groove of the placement rack (3); The fixing piece is connected to the other end of the fixing plate (5), is sleeved on the placement frame (3), and is in contact with the rubber surface (301).

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