Kit for detecting homocysteine in urine

Abstract

The utility model discloses a kit for detecting homocysteine in urine, which comprises an external test tube, a detection component is arranged in an inner cavity of a box component, the detection component comprises a spiral tube, and the bottom of the spiral tube is arranged at the bottom of the inner cavity of the box component; the first reactant is movably stacked on the top of the inner cavity of the spiral pipe; the second reactant is movably stacked in the middle of the inner cavity of the spiral pipe; the reaction electrode is fixedly arranged at the bottom of an inner cavity of the spiral pipe, and the inner cavity of the spiral pipe is provided with a plurality of blocking mechanisms used for stopping liquid. Through the arrangement mode that the detection assembly is matched with the plugging mechanism, specific combination of enzyme and a substrate is adopted in measurement, so that the sensitivity is steadily improved, the detection time is greatly shortened, the measurement operation is simplified, radioactivity is avoided, the cost is greatly reduced, and the stability of the device is improved; and the popularization value is extremely high.

Description

Technical Field

[0001] The utility model relates to the technical field of medical reagent kits, in particular to a reagent kit for detecting homocysteine ​​in urine. Background Art

[0002] Homocysteine ​​is a sulfur-containing amino acid that cannot be synthesized by the human body itself. It usually relies on the decomposition of methionine to be produced, and it is not an essential amino acid contained in protein. In a healthy human body, the content of homocysteine ​​in serum is generally low, and it is mostly in the form of protein binding. Homocysteine ​​can change the permeability of blood vessel walls and is an independent risk factor for atherosclerosis and stroke. It is found that the content of homocysteine ​​in urine will also increase when myocardial infarction occurs. Therefore, we advocate the determination of homocysteine ​​content in urine and the development of a device that can detect homocysteine ​​in urine.

[0003] At present, the main methods for measuring homocysteine ​​are: amino acid analysis, paper chromatography, quantitative analysis by amino acid analyzer, isotope method, radioenzyme analysis, enzyme conversion immunoassay, gas chromatography-mass spectrometry (GC-MS), homocysteine ​​cycle enzyme method, immunological method, flow injection analysis (FLA), electrospray ionization mass spectrometry, direct color reaction, radioimmunoassay, etc. However, the current methods for detecting homocysteine ​​are mainly focused on: taking a long time. On the one hand, it is due to the method. The instruments required for amino acid analysis and paper chromatography are relatively complex, which greatly prolongs the detection time of homocysteine ​​and is cumbersome to operate. Radioactive elements are added to some experimental designs. This radioactive element will decay. If it is not handled properly, it is easy to cause radioactive contamination and is not suitable for promotion and use. In addition, the experimental process requires both the conversion of the substrate and the determination of the relevance of the substrate. The operation cycle is long and the operation technology is complicated. Because it involves the use of some precision instruments, such as gas chromatographs, it consumes a lot of resources and it is difficult to achieve the expected effect. Utility Model Content

[0004] The purpose of the utility model is to provide a kit for detecting homocysteine ​​in urine to solve the problems raised in the above background technology.

[0005] To achieve the above object, the utility model provides the following technical solution: a kit for detecting homocysteine ​​in urine, comprising: an external test tube, one end of which is movably provided with a box assembly, the inner cavity of which is provided with a detection assembly, and the detection assembly comprises:

[0006] A spiral tube, the bottom of which is fixedly inserted into the bottom of the inner cavity of the box assembly;

[0007] A first reactant, the first reactant is movably stacked on the top of the inner cavity of the spiral tube;

[0008] A second reactant, the second reactant is movably stacked in the middle of the inner cavity of the spiral tube;

[0009] The reaction electrode is fixedly arranged at the bottom of the inner cavity of the spiral tube, and the inner cavity of the spiral tube is provided with a plurality of blocking mechanisms for liquid cutoff.

[0010] Preferably, the box assembly comprises:

[0011] The bottom of the spiral tube is fixedly connected with the bottom of the box body, and a feed port is provided at one end of the top of the box body;

[0012] A display screen controller, the display screen controller is fixedly inserted into the other end of the top of the box body;

[0013] A push-pull plate, the push-pull plate slidingly inserted into one side of the inner wall of the feed port;

[0014] A plurality of supporting legs are respectively connected to two sides of the bottom of the box body.

[0015] Preferably, the push-pull plate is used to seal the inner cavity of the feed port, and the top of the spiral tube is arranged in the middle of the inner cavity of the feed port.

[0016] Preferably, a waste liquid collecting pipe is movably sleeved on the outer wall of the bottom of the spiral tube, and the waste liquid collecting pipe is arranged between two adjacent supporting legs.

[0017] Preferably, the blocking mechanism comprises:

[0018] A sealing plate, the sealing plate rotates in the inner cavity of the spiral tube;

[0019] A micro electric telescopic rod, wherein a plurality of micro electric telescopic rods are respectively fixed and bonded to the top, middle and bottom of the inner cavity of the spiral tube;

[0020] A connecting assembly is used for the fixed-axis rotation of the sealing plate.

[0021] Preferably, the connection assembly comprises:

[0022] A fixing plate, wherein the fixing plate is fixed to the inner wall of the spiral tube;

[0023] A connecting block, two ends of which are respectively rotatably connected with two ends of the bottom of the fixing plate, and one side of the connecting block is fixedly connected with one side of the sealing plate;

[0024] A torsion spring is sleeved on the middle part of the connecting block, one end of the torsion spring is fixed to the middle part of the fixing plate, and the other end of the torsion spring is fixed to one side of the adjacent sealing plate.

[0025] Technical effects and advantages of the utility model:

[0026] The utility model adopts the arrangement mode of matching the detection component and the blocking mechanism, and adopts the specific combination of enzyme and substrate in the measurement through the flow and reaction of urine in the inner cavity of the spiral tube, so that the sensitivity is steadily improved, the detection time is greatly shortened, the measurement operation is simplified, and it is non-radioactive. It is designed according to the environmental protection principles of green, pollution-free and degradable during use. The instrument uses cost-effective consumables in the use of materials, which greatly reduces the cost and improves the stability of the device. It has extremely high promotion value. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 It is a schematic diagram of the overall structure of the utility model.

[0028] Figure 2 It is a schematic diagram of the side sectional structure of the box body of the utility model.

[0029] Figure 3 It is a schematic diagram of the side sectional structure of the spiral tube of the utility model.

[0030] Figure 4 It is a schematic diagram of the cross-sectional structure of the sealing plate of the utility model.

[0031] In the figure: 1. external test tube; 2. box assembly; 21. box body; 22. display screen controller; 23. push-pull plate; 24. support leg; 3. waste liquid collection tube; 4. detection assembly; 41. spiral tube; 42. first reactant; 43. second reactant; 44. reaction electrode; 5. blocking mechanism; 51. sealing plate; 52. micro electric telescopic rod; 53. connecting assembly; 531. fixing plate; 532. connecting block; 533. torsion spring. DETAILED DESCRIPTION

[0032] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0033] The utility model provides Figure 1-4The kit for detecting homocysteine ​​in urine shown in the figure comprises: an external test tube 1, one end of which is movably provided with a box assembly 2, the box assembly 2 comprises: a box body 21, a display screen controller 22, a push-pull plate 23 and a plurality of support legs 24, the bottom of the spiral tube 41 is fixedly connected with the bottom of the box body 21, the box body 21 is made of polycarbonate PC, which is transparent, high-strength, impact-resistant and friction-resistant, and is convenient for repeated use of the kit, a feed port is provided at one end of the top of the box body 21, and the display screen controller 2 2 is fixedly inserted at the other end of the top of the box body 21, and the push-pull plate 23 is slidably inserted at one side of the inner wall of the feed port. The push-pull plate 23 is used to seal the inner cavity of the feed port, and a magnetic suction component is connected between the push-pull plate 23 and one end of the feed port, so that the push-pull plate 23 will not slide randomly when the box body 21 is closed, and is used for the push-pull plate 23 to stably seal the inner cavity of the box body 21. A plurality of support legs 24 are respectively connected to the two sides of the bottom of the box body 21, and the plurality of support legs 24 are two support legs 24, so that the box body 21 can be stably supported on a fixed desktop;

[0034] The inner cavity of the box component 2 is provided with a detection component 4, which includes: a spiral tube 41, a first reactant 42, a second reactant 43 and a reaction electrode 44. The spiral tube 41 has a smooth inner wall and a multi-helix structure with a gentle angle. The spiral tube 41 is made of super-hydrophobic PDMS material, has a hydrophobic effect, and is not easy to adhere to urine. The inner cavity of the spiral tube 41 has an inclination angle of 14.38 degrees, which can slow down the flow rate of the reaction, so that the mixed liquid reacts fully, and also avoids the phenomenon of accumulation due to turbulent flow, and the multi-helix structure of the spiral tube 41 can increase The reaction area of ​​the mixture and the enzyme is large, so that the mixed solution can be fully reacted, the first reactant 42 is cystathionine-β-synthase, the second reactant 43 is cystathionine β-lyase and lactate dehydrogenase, the reaction electrode 44 is a lactate oxidase electrode, the first reactant 42 and the second reactant 43 can be arranged along the bottom inner wall of different parts of the spiral tube 41, and the first reactant 42, the second reactant 43 and the reaction electrode 44 are respectively fixed in the inner cavity of the spiral tube 41 by the enzyme-MOF method embedded in the metal organic framework, so that the outer The urine in the inner cavity of the test tube 1 is poured into the inner cavity at the top of the spiral tube 41, so that the urine reacts with cystathionine-β-synthase, cystathionine β-lyase and lactate dehydrogenase in sequence for a certain period of time. The bottom of the spiral tube 41 is fixedly inserted into the bottom of the inner cavity of the box assembly 2, the top of the spiral tube 41 is arranged in the middle of the inner cavity of the feed port, the first reactant 42 is movably stacked on the top of the inner cavity of the spiral tube 41, the second reactant 43 is movably stacked in the middle of the inner cavity of the spiral tube 41, the reaction electrode 44 is fixedly arranged at the bottom of the inner cavity of the spiral tube 41, and the lactate oxidase The lactate oxidase in the electrode is a specific enzyme that can catalyze the oxidation reaction of L-lactic acid and generate a corresponding electrochemical signal. Two wires are passed through the outer periphery of the spiral tube 41. After the reaction mixture reacts in the spiral tube 41, when it passes through the reaction electrode 44, a current is generated at the reaction electrode 44 through an oxidation-reduction reaction, and a definite indication is displayed on the external display controller 22 through the wire. The user can read a specific value on the display screen of the display controller 22 to refer to whether his homocysteine ​​is elevated;

[0035] The outer wall of the bottom of the spiral tube 41 is movably sleeved with a waste liquid collection pipe 3, which is arranged between two adjacent support legs 24. When the mixed waste liquid in the inner cavity of the spiral tube 41 needs to be discharged, the waste liquid collection pipe 3 is sleeved on the bottom of the spiral tube 41 so that the waste liquid in the inner cavity of the spiral tube 41 can be discharged stably. When the spiral tube 41 is not in use, both ends of the spiral tube 41 are sealed by plugs to keep the inner cavity of the spiral tube 41 in a closed environment to reduce bacterial contamination.

[0036] The inner cavity of the spiral tube 41 is provided with a plurality of blocking mechanisms 5 for liquid cutoff, and the blocking mechanism 5 comprises: a sealing plate 51, a micro electric telescopic rod 52 and a connecting assembly 53, the sealing plate 51 rotates in the inner cavity of the spiral tube 41, and the outer wall of the sealing plate 51 is made of a highly elastic rubber ring material, and the outer wall of the sealing plate 51 can be pressed against the inner wall of the spiral tube 41, so that the sealing plate 51 can block and isolate the inner cavity of the spiral tube 41, and the plurality of micro electric telescopic rods 52 are respectively fixedly bonded to the top, middle and bottom of the inner cavity of the spiral tube 41, and one end of the micro electric telescopic rod 52 is fixed to the inner wall of the spiral tube 41 (such as the attached Figure 3 As shown in the figure, the micro electric telescopic rod 52 is an oblique fixed structure, so that the micro electric telescopic rod 52 can push one side of the sealing plate 51, so that the sealing plate 51 is flipped, so that the mixed liquid on the other side of the sealing plate 51 can flow through the sealing plate 51 in the inner cavity of the spiral tube 41;

[0037] The connecting assembly 53 is used for the fixed-axis rotation of the sealing plate 51. The connecting assembly 53 includes: a fixed plate 531, a connecting block 532 and a torsion spring 533. The fixed plate 531 is fixed to the inner wall of the spiral tube 41. The fixed plate 531 is an L-shaped structure. The connecting block 532 is fixed by three cylindrical structures. The two ends of the connecting block 532 are respectively rotatably interlaced and connected with the two ends of the bottom of the fixed plate 531. One side of the connecting block 532 is fixedly connected to one side of the sealing plate 51. The torsion spring 533 is sleeved on the middle part of the connecting block 532. One end of the torsion spring 533 is fixed to the middle part of the fixed plate 531, and the other end of the torsion spring 533 is fixed to one side of the adjacent sealing plate 51. After the sealing plate 51 is pushed to a certain angle by the torsion spring 533, after the micro-electric telescopic rod 52 releases the thrust, the sealing plate 51 can be elastically restored to the original sealing state of the spiral tube 41 through the elasticity of the torsion spring 533.

[0038] When in use, the user injects urine into the inner cavity of the external test tube 1 and mixes it evenly, pulls the push-pull plate 23 to expose the top of the spiral tube 41, pours the liquid in the inner cavity of the external test tube 1 into the inner cavity of the spiral tube 41, flows into the first reactant 42 for one minute of mixing, drives the micro-electric telescopic rod 52 located at the top of the spiral tube 41 to open the sealing plate 51 located at the top of the spiral tube 41, and the mixed liquid continues to flow to the second reactant 43 for reaction. After waiting for two minutes, the sealing plate 51 located in the middle of the spiral tube 41 is opened, and the mixture reacts with the reaction electrode 44. After waiting for two minutes, the sealing plate 51 at the bottom of the spiral tube 41 is opened, and the liquid flows out from the inner cavity of the waste liquid collection tube 3. Excretion, the display controller 22 displays the data of homocysteine. Through the flow and reaction of urine in the inner cavity of the spiral tube 41, the specific combination of enzyme and substrate is adopted in the measurement, so that the sensitivity is steadily improved, the detection time is greatly shortened, and the measurement operation is simplified. The user only needs to mix the urine in the test tube and then pour it into the device to read a specific value, which greatly simplifies the experimental operation and is easier to promote to most non-scientific researchers. It is non-radioactive and is designed according to the green, pollution-free and degradable environmental protection principles during use. The instrument uses cost-effective consumables in the use of materials, which greatly reduces costs and improves the stability of the device, and has extremely high promotion value.

[0039] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A kit for detecting homocysteine ​​in urine, comprising: An external test tube (1), characterized in that a box assembly (2) is movably provided at one end of the external test tube (1), a detection assembly (4) is provided in the inner cavity of the box assembly (2), and the detection assembly (4) comprises: A spiral tube (41), the bottom of the spiral tube (41) being fixedly inserted into the bottom of the inner cavity of the box assembly (2); A first reactant (42), the first reactant (42) being movably stacked on the top of the inner cavity of the spiral tube (41); A second reactant (43), the second reactant (43) being movably stacked in the middle of the inner cavity of the spiral tube (41); A reaction electrode (44), the reaction electrode (44) being fixedly arranged at the bottom of the inner cavity of the spiral tube (41), and the inner cavity of the spiral tube (41) being provided with a plurality of blocking mechanisms (5) for liquid cutoff.

2. A kit for detecting homocysteine ​​in urine according to claim 1, characterized in that: The box assembly (2) comprises: A box body (21), wherein the bottom of the spiral tube (41) is fixedly connected to the bottom of the box body (21), and a feed port is provided at one end of the top of the box body (21); A display screen controller (22), the display screen controller (22) being fixedly inserted into the other end of the top of the box body (21); A push-pull plate (23), the push-pull plate (23) being slidably inserted into one side of the inner wall of the feed inlet; A plurality of supporting legs (24), wherein the plurality of supporting legs (24) are respectively connected to two sides of the bottom of the box body (21).

3. A kit for detecting homocysteine ​​in urine according to claim 2, characterized in that: The push-pull plate (23) is used to seal the inner cavity of the feed port, and the top of the spiral tube (41) is arranged in the middle of the inner cavity of the feed port.

4. A kit for detecting homocysteine ​​in urine according to claim 2, characterized in that: A waste liquid collection pipe (3) is movably sleeved on the outer wall of the bottom of the spiral tube (41), and the waste liquid collection pipe (3) is arranged between two adjacent support legs (24).

5. A kit for detecting homocysteine ​​in urine according to claim 2, characterized in that: The blocking mechanism (5) comprises: A sealing plate (51), wherein the sealing plate (51) rotates in the inner cavity of the spiral tube (41); A micro electric telescopic rod (52), wherein a plurality of the micro electric telescopic rods (52) are respectively fixedly bonded to the top, middle and bottom of the inner cavity of the spiral tube (41); A connecting assembly (53), wherein the connecting assembly (53) is used for the fixed-axis rotation of the sealing plate (51).

6. A kit for detecting homocysteine ​​in urine according to claim 5, characterized in that: The connection component (53) comprises: A fixing plate (531), wherein the fixing plate (531) is fixed to the inner wall of the spiral tube (41); A connecting block (532), wherein two ends of the connecting block (532) are respectively rotatably connected to two ends of the bottom of the fixing plate (531), and one side of the connecting block (532) is fixedly connected to one side of the sealing plate (51); A torsion spring (533), wherein the torsion spring (533) is sleeved on the middle portion of the connecting block (532), one end of the torsion spring (533) is fixed to the middle portion of the fixing plate (531), and the other end of the torsion spring (533) is fixed to one side of an adjacent sealing plate (51).

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