Detection card

Abstract

The utility model relates to a detection card which comprises a card box, the top wall of the card box is provided with a sample adding port and an observation window, a liquid channel is arranged in the card box, the liquid channel comprises an antibody arranged at the position right opposite to the observation window and a fluorescent material located on the upstream of the antibody, and the sample adding port and the observation window are sequentially arranged in the length direction of the liquid channel; the blood sampling mechanism is arranged on the side wall of the clamping box, the blood sampling mechanism comprises a needle body capable of puncturing a position to be sampled, and the blood sampling mechanism can be switched between a working state in which a needle head of the needle body is exposed and a protection state in which the needle body is hidden; the quantitative sampler is detachably connected to the side wall of the clamping box and can collect a preset amount of samples, and the samples in the quantitative sampler can enter the liquid channel through the sample adding opening. The detection card integrates whole blood quantification and detection, so that a user can conveniently detect at home, the possibility of sample pollution is reduced, and meanwhile, the fear of the user can be relieved.

Description

Technical Field

[0001] The utility model relates to the technical field of in vitro diagnosis, in particular to a detection card. Background Art

[0002] Currently, many tests are done by drawing blood from the finger, but under the current blood collection method, blood collection is a painful process for patients who are afraid of the blood collection needle. Although medical diagnosis and treatment methods are constantly advancing, the current blood collection method still ignores people's needs for medical care - a more comfortable and safer blood collection method.

[0003] In addition, current blood collection tubes usually only realize the function of blood collection. After blood collection, the sampled blood is transferred to a detection device for detection. This is not convenient for users to detect by themselves at home. At the same time, the possibility of sample contamination is increased during the transfer process. Utility Model Content

[0004] One purpose of the utility model is to provide a detection card to at least solve one of the above technical problems.

[0005] To achieve the above-mentioned purpose, the first aspect of the present invention provides a detection card, comprising:

[0006] A card box, wherein a sample addition port and an observation window are provided on the top wall of the card box, a liquid channel is provided in the card box, the liquid channel comprises an antibody arranged at a position directly opposite to the observation window, and a fluorescent material located upstream of the antibody, and the sample addition port and the observation window are sequentially arranged along the length direction of the liquid channel;

[0007] A blood sampling mechanism is arranged on the side wall of the cartridge, the blood sampling mechanism comprises a needle body capable of puncturing a position to be blood sampled, and the blood sampling mechanism can be switched between a working state in which the needle head of the needle body is exposed and a protective state in which the needle body is hidden;

[0008] The quantitative sampler is detachably connected to the side wall of the cartridge and can collect a preset amount of sample. The sample in the quantitative sampler can enter the liquid channel through the sample addition port.

[0009] Optionally, the blood sampling mechanism includes:

[0010] A fixed guide sleeve connected to the side wall of the card box;

[0011] A protective sleeve is at least partially slidably disposed in the fixed guide sleeve, and the protective sleeve can be located in a protection position and a working position by sliding along the fixed guide sleeve. The needle body is disposed in the protective sleeve, and the end of the needle body is fixed to the cartridge. The needle extends out of the fixed guide sleeve, and the protective sleeve is located in the working position. The needle extends out of the protective sleeve, so that the blood collection mechanism is in a working state. The protective sleeve is located in the protection position, and the needle is located in the protective sleeve, so that the blood collection mechanism is in the protection state.

[0012] A spring is arranged in the fixed guide sleeve, and the spring can drive the protective sleeve to be located at the protective position.

[0013] Optionally, the fixed guide sleeve is provided with a guide groove, and the guide groove extends along the sliding direction of the protective sleeve;

[0014] The protective cover comprises:

[0015] A sleeve head is at least partially slidably disposed in the fixed guide sleeve, and the spring is disposed between the side wall of the cartridge and the end surface of the sleeve head;

[0016] A guide plate connected to the sleeve and slidably disposed in the guide groove;

[0017] A stopper is connected to one end of the guide plate, and the stopper is slidably disposed in the card box and can abut against the inner wall of the card box.

[0018] Optionally, the quantitative sampler comprises:

[0019] A quantitative part, the quantitative part comprising a whole blood quantitative chamber, the whole blood quantitative chamber having an inlet and an outlet opposite to each other and a terminal port;

[0020] The hand-held part is connected with the quantitative part, and the hand-held part is provided with a capillary force release port, and the capillary force release port is communicated with the terminal port.

[0021] Optionally, a side wall of the card box is provided with an insertion hole, and the quantitative portion can be inserted into the insertion hole.

[0022] Optionally, the top wall of the cartridge is further provided with a reagent port, and the reagent port, the sample addition port and the observation window are sequentially arranged along the length direction of the liquid channel;

[0023] The detection card further comprises a reagent pack. The top surface of the card box is connected to a limiting portion. The reagent pack can be arranged on the limiting portion. The reagent in the reagent pack can enter the liquid channel through the reagent port.

[0024] Optionally, the reagent kit includes:

[0025] A tube body, wherein the tube body can contain a reagent;

[0026] A sealing film, wherein the sealing film sealing cover is arranged on the tube opening of the tube body;

[0027] A puncture portion is arranged in the card box, the reagent port is arranged opposite to the puncture portion, the tube body can be inserted into the reagent port, and the puncture portion can puncture the sealing film.

[0028] Optionally, the limiting portion is a hollow structure with openings at both ends, and the limiting portion is arranged opposite to the reagent port so that the reagent package can be inserted into the limiting portion and the reagent port.

[0029] Optionally, the reagent package further includes an elastic positioning portion, which is sleeved outside the tube body, and there is a gap between the outer wall of the tube body and the inner wall of the limiting portion, and the elastic positioning portion and the inner wall of the limiting portion are interference fit.

[0030] Optionally, a liquid guide portion is provided on the inner side of the bottom wall of the cartridge, the puncturing portion is provided in the liquid guide portion, and a liquid outlet end of the liquid guide portion is adjacent to a liquid inlet end of the liquid channel.

[0031] Optionally, the card box includes:

[0032] A box body, the upper end of which is open;

[0033] The upper cover is arranged on the opening of the box body, the sample adding port and the observation window are both opened on the upper cover, and the liquid channel is located between the box body and the upper cover.

[0034] Optionally, the liquid channel further includes a test strip, and the antibody and the fluorescent material are arranged on the test strip.

[0035] Optionally, the card box further includes a fixing protrusion, wherein the fixing protrusion is connected to the top wall of the upper cover, and the fixing protrusion can be pressed against the upper side of the test strip.

[0036] Optionally, a guide groove is further provided on the top wall of the upper cover, the sample addition port is connected to the guide groove and is sequentially arranged along the flow direction of the liquid, and / or

[0037] The upper cover is also connected to a first transverse column, which is located between the upstream of the observation window and the downstream of the fluorescent material. The first transverse column is used to slow down the flow rate of the liquid on the test strip.

[0038] Optionally, the liquid channel further comprises a filter membrane, a fluorescent area, a valve area and an antibody coating area which are arranged on the bottom wall of the box body and arranged in sequence;

[0039] The filter membrane is arranged opposite to the sample injection port and is used to filter red blood cells. The fluorescent area is used to fix the fluorescent material containing antibodies. The valve area is used to prolong the time for liquid to flow into the antibody coated area. The antibody coated area is arranged opposite to the observation window and is used to fix the antibodies.

[0040] Optionally, the valve area includes a plurality of second transverse columns, the second transverse columns extend along the width direction of the liquid channel, and the plurality of second transverse columns extend along the length direction of the liquid channel; and / or

[0041] The fluorescent area includes a plurality of first protrusions arranged at intervals; and / or

[0042] The antibody coating area includes a plurality of second protrusions arranged at intervals.

[0043] Optionally, a plurality of the first protrusions are arranged in a plurality of rows, the plurality of rows of the first protrusions are sequentially arranged along the length direction of the liquid channel, and two adjacent rows of the first protrusions are staggered in the width direction of the liquid channel; and / or

[0044] A plurality of the second protrusions are arranged in a plurality of rows, the plurality of rows of the second protrusions are sequentially arranged along the length direction of the liquid channel, and two adjacent rows of the second protrusions are staggered in the width direction of the liquid channel.

[0045] Optionally, the first protrusion and / or the second protrusion is a hemispherical structure; and / or

[0046] The height of the first protrusion and / or the second protrusion is 2-50 μm; and / or

[0047] The height of the second cross column is 10-80 μm.

[0048] Optionally, a first anti-backflow zone is provided between the filter membrane and the fluorescent zone, and a height of the first anti-backflow zone is higher than a height of the fluorescent zone; and / or

[0049] A second anti-backflow zone is arranged between the valve zone and the antibody coating zone, and the height of the second anti-backflow zone is higher than that of the valve zone.

[0050] Optionally, a drainage structure is provided between the first anti-backflow zone and the filter membrane, the drainage structure comprising a plurality of continuously connected arc-shaped protrusions, the arc-shaped protrusions protruding toward the filter membrane; and / or

[0051] A sawtooth-shaped deceleration structure is arranged between the first anti-backflow area and the fluorescent area.

[0052] Optionally, the liquid channel further includes a waste liquid zone, wherein the waste liquid zone is located downstream of the antibody coating zone, and the height of the waste liquid zone is lower than the height of the antibody coating zone.

[0053] Optionally, the fluorescent area, the valve area and the antibody coating area are integrally formed with the box body.

[0054] Optionally, the card box further includes a first stop bar and a second stop bar, the first stop bar and the second stop bar are arranged at intervals along the width direction of the liquid channel, and the first stop bar and the second stop bar both extend along the length direction of the liquid channel;

[0055] The liquid passage is formed between the first stopper strip and the second stopper strip.

[0056] Optionally, the card box also includes an upper stop portion connected to the upper cover, the upper stop portion is transparent at least at a position opposite to the observation window, the upper stop portion is provided with a avoidance opening, the avoidance opening is arranged opposite to the sample addition port, the lower surface of the upper stop portion is in contact with the first stop bar and the second stop bar, and the upper stop portion, the first stop bar, the second stop bar and the bottom of the box body form the liquid channel.

[0057] As can be seen from the above, the technical solution provided by the utility model is that when using the test card, first use the blood collection mechanism to pierce the finger, remove the quantitative sampler, and adsorb a preset amount of sample on the finger; transfer the quantitative sampler to the sample addition port, and the sample enters the liquid channel through the sample addition port; the sample flows to the antibody, and since the antibody is arranged opposite to the observation window, the structure can be observed at the observation window.

[0058] When it is necessary to prick a finger, the blood collection mechanism can be in a working position, exposing the needle, and the needle body can prick the finger; before and after blood collection, the blood collection mechanism is in a protective state, the needle body is hidden, and the user cannot see the needle body. Therefore, during the entire process of blood collection, the user cannot see the needle body, thereby reducing the user's fear and the risk of accidental injury. At the same time, the structure of the blood collection mechanism can also prevent the needle body from being contaminated.

[0059] The quantitative sampler is detachably connected to the side wall of the card box, so that during the transportation of the test card and before the test card is used, the quantitative sampler can be fixed on the card box to prevent the quantitative sampler from moving and being contaminated. The quantitative sampler can collect a preset amount of sample, so that non-professionals can collect a sufficient amount of sample. BRIEF DESCRIPTION OF THE DRAWINGS

[0060] Figure 1 It is a structural schematic diagram of the first detection card provided by the embodiment of the utility model;

[0061] Figure 2 This is an exploded view of the first detection card provided by the embodiment of the utility model;

[0062] Figure 3 Yes Yes Figure 2 A partial enlarged view of the middle A;

[0063] Figure 4 It is a cross-sectional view of the first detection card provided by the embodiment of the utility model;

[0064] Figure 5 It is a structural schematic diagram of a blood sampling mechanism provided by an embodiment of the utility model;

[0065] Figure 6 It is a cross-sectional view of another position of a part of the first detection card provided by the embodiment of the utility model;

[0066] Figure 7 This is a schematic diagram of the structure of the first detection card with a hidden upper cover and a reagent pack provided by an embodiment of the utility model;

[0067] Figure 8 Yes Yes Figure 7 A partial enlarged view of point B in the middle;

[0068] Fig. 9 This is a schematic structural diagram of the upper cover of the first detection card provided by the embodiment of the utility model;

[0069] Fig.10 It is a structural schematic diagram of a second detection card provided by an embodiment of the utility model;

[0070] Fig.11 This is an exploded view of the second detection card provided by the embodiment of the utility model;

[0071] Fig.12 It is a structural schematic diagram of the upper cover of the second detection card provided by the embodiment of the utility model;

[0072] Fig.13 is a cross-sectional view of a second detection card provided by an embodiment of the utility model;

[0073] Fig.14 is a cross-sectional view of another position of the second detection card provided by an embodiment of the utility model;

[0074] Fig.15 This is a schematic diagram of the structure of the second detection card with a hidden upper cover and a reagent pack provided by an embodiment of the utility model;

[0075] Fig.16 Yes Yes Fig.15 A partial enlarged view of point C in the middle;

[0076] Fig.17 Yes Yes Fig.15 A partial enlarged view of point D in the middle;

[0077] Fig.18 It is a partial schematic diagram of the fluorescent area or antibody coating area of ​​the second detection card provided in the embodiment of the utility model.

[0078] In the figure:

[0079] 1. Card box;

[0080] 11. Limiting part;

[0081] 12. Upper cover; 121. First card protrusion; 122. Reagent port; 123. Sample adding port; 124. Observation window; 125. Guide groove; 127. Second anti-slip protrusion;

[0082] 13. Box body; 131. First bayonet; 132. Socket;

[0083] 14. Liquid guide; 15. Fixing protrusion; 16. First horizontal column; 17. First stop bar; 18. Second stop bar;

[0084] 19. Test paper limit assembly; 191. Third stop bar; 192. Fourth stop bar; 193. Limit protrusion;

[0085] 101, upper stopper; 1011, avoidance opening; 102, puncture portion; 103, second clamping protrusion; 104, third clamping protrusion;

[0086] 2. Liquid channel; 21. Test strip; 211. Sample pad; 212. Fluorescent marker pad; 213. Glass cellulose membrane; 214. Absorbent paper; 22. Filter membrane; 23. Fluorescent area; 231. First protrusion; 24. Valve area; 241. Second horizontal column; 25. Antibody coating area; 251. Second protrusion; 26. First anti-backflow area; 27. Drainage structure; 28. Sawtooth deceleration structure; 29. ​​Waste liquid area; 201. Second anti-backflow area;

[0087] 3. Blood collection mechanism; 31. Fixed guide sleeve; 311. Guide groove; 32. Protective sleeve; 321. Sleeve head; 322. Guide plate; 323. Stopper; 33. Spring; 34. Fixed block; 35. Needle body;

[0088] 4. quantitative sampler; 41. quantitative part; 42. hand-held part; 421. first anti-slip protrusion; 422. capillary force release port;

[0089] 5. Reagent pack; 51. Tube body; 52. Sealing film; 53. Elastic positioning part. DETAILED DESCRIPTION

[0090] The technical solution of the utility model is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the utility model, rather than to limit the utility model. It is also necessary to explain that, for the convenience of description, only the parts related to the utility model are shown in the accompanying drawings, rather than all.

[0091] Some directional words are defined in the present utility model. Unless otherwise specified, the directional words used, such as "upper", "lower", "left", "right", "inside" and "outside", are used for ease of understanding and therefore do not constitute limitations on the protection scope of the present utility model.

[0092] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0093] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0094] Embodiment 1

[0095] This embodiment provides a test card that integrates whole blood quantification and detection, so that users can perform self-tests at home and reduce the possibility of sample contamination.

[0096] like Figure 1 and Figure 2 As shown, the detection card provided in this embodiment includes a card box 1, a blood sampling mechanism 3 and a quantitative sampler 4. The top wall of the card box 1 is provided with a sample injection port 123 and an observation window 124. A liquid channel 2 is provided in the card box 1. The liquid channel 2 includes an antibody arranged at a position directly opposite to the observation window 124, and a fluorescent material located upstream of the antibody. Exemplarily, the antibody can be a specific antibody for the detection antigen, and the fluorescent material can also contain the antibody.

[0097] The sample addition port 123 and the observation window 124 are sequentially arranged along the length direction of the liquid channel 2. The blood collection mechanism 3 is arranged on the side wall of the cartridge 1, and the blood collection mechanism 3 includes a needle body 35 that can puncture the position to be blood collected, and the blood collection mechanism 3 can switch between a working state in which the needle head of the needle body 35 is exposed and a protection state in which the needle body 35 is hidden. When it is necessary to puncture a finger, the blood collection mechanism 3 can be in a working position, exposing the needle head, and the needle body 35 can puncture the finger; before and after blood collection, the blood collection mechanism 3 is in a protection state, the needle body 35 is hidden, and the user will not see the needle body 35. Therefore, during the entire process of blood collection, the user will not see the needle body 35, thereby reducing the user's fear and reducing the risk of accidental injury. At the same time, the structure of the blood collection mechanism 3 can also prevent the needle body 35 from being contaminated.

[0098] The quantitative sampler 4 is detachably connected to the side wall of the cartridge 1, so that during the transportation of the test card and before the test card is used, the quantitative sampler 4 can be fixed on the cartridge 1 to prevent the quantitative sampler 4 from moving and being contaminated. The quantitative sampler 4 can collect a preset amount of sample, making it convenient for non-professionals to collect a sufficient amount of sample. The sample in the quantitative sampler 4 can enter the liquid channel 2 through the sample addition port 123.

[0099] When using the test card, first use the blood collection mechanism 3 to prick the finger, remove the quantitative sampler 4, and adsorb a preset amount of sample on the finger; transfer the quantitative sampler 4 to the sample port 123, and the sample enters the liquid channel 2 through the sample port 123; the sample flows to the antibody, and since the antibody is arranged opposite to the observation window 124, the structure can be observed at the observation window 124.

[0100] like Figure 2 and Fig. 9 As shown, the top wall of the cartridge 1 may also be provided with a reagent port 122, and the reagent port 122, the sample addition port 123 and the observation window 124 are sequentially arranged along the length direction of the liquid channel 2. The test card further includes a reagent pack 5, and the top surface of the cartridge 1 is connected to a limiting portion 11, and the reagent pack 5 can be arranged in the limiting portion 11, and the reagent in the reagent pack 5 can enter the liquid channel 2 through the reagent port 122.

[0101] The reagent in the reagent pack 5 can dilute the sample, thereby facilitating the sample to flow to the observation window 124. After the quantitative sampler 4 is transferred to the sample adding port 123, the reagent pack 5 can be opened, and the reagent in the reagent pack 5 can be added to the liquid channel 2 through the reagent port 122. The reagent flows in the liquid channel 2 and flows to the sample, thereby diluting the sample.

[0102] Optionally, the cartridge 1 includes a box body 13 and an upper cover 12, the upper end of the box body 13 is open, the upper cover 12 is covered on the opening of the box body 13, the reagent port 122, the sample injection port 123 and the observation window 124 are all provided on the upper cover 12, and the liquid channel 2 is located between the box body 13 and the upper cover 12. The cartridge 1 is provided in a split body and forms the liquid channel 2. In this embodiment, the top wall of the cartridge 1 is the top wall of the upper cover 12, the side wall of the cartridge 1 can be the side wall of the box body 13, and the bottom wall of the cartridge 1 can be the bottom wall of the box body 13.

[0103] like Figure 2 As shown, specifically, the box body 13 and the upper cover 12 are snap-fitted, so as to facilitate the assembly of the card box 1. Optionally, the box body 13 is provided with a first snap-fit ​​port 131, and the upper cover 12 is provided with a first snap-fit ​​protrusion 121 (such as Fig. 9 As shown in FIG. 1 , the first locking protrusion 121 can be locked in the first locking opening 131. Of course, in other optional embodiments, the upper cover 12 may be provided with the first locking opening 131, and the box body 13 may be provided with the first locking protrusion 121.

[0104] like Figure 2-Figure 4 As shown, the blood sampling mechanism 3 includes a fixed guide sleeve 31, a protective sleeve 32 and a spring 33. The fixed guide sleeve 31 is used to guide the protective sleeve 32. The sliding of the protective sleeve 32 can make the needle of the needle body 35 extend and hide the needle body 35. The spring 33 applies a force to the protective sleeve 32. Specifically, the fixed guide sleeve 31 is connected to the side wall of the cartridge 1. The protective sleeve 32 is at least partially slidably disposed in the fixed guide sleeve 31. The fixed guide sleeve 31 can be a sliding guide for the protective sleeve 32. The protective sleeve 32 slides along the fixed guide sleeve 31 to be located in the protection position and the working position.

[0105] The needle body 35 is disposed in the protective sleeve 32, the end of the needle body 35 is fixed to the cartridge 1, the needle extends out of the fixed guide sleeve 31, the protective sleeve 32 is located in the working position, the needle extends out of the protective sleeve 32, so that the blood collection mechanism 3 is in the working state, the protective sleeve 32 is located in the protection position, and the needle is located in the protective sleeve 32, so that the blood collection mechanism 3 is in the protection state. The spring 33 is disposed in the fixed guide sleeve 31, and the spring 33 can drive the protective sleeve 32 to be located in the protection position. It can be understood that the spring 33 is always in a compressed state.

[0106] Before sampling, the protective cover 32 is in a protective position (eg Figure 4As shown in the figure, the needle head of the needle body 35 is located in the protective sleeve 32. When it is necessary to pierce the finger, the end of the protective sleeve 32 (the end away from the card box 1) is pressed against the finger, pressing the detection card, and the force between the protective sleeve 32 and the finger increases, overcoming the force of the spring 33 on the protective sleeve 32. The spring 33 is continuously compressed, and the protective sleeve 32 slides in the fixed guide sleeve 31. The protective sleeve 32 is in the working position, and the needle head of the needle body 35 is located outside the protective sleeve 32. Since the detection card is continuously pressed down, the needle body 35 can pierce the finger. After the blood collection is completed, release the finger, the spring 33 returns to deformation, and the protective sleeve 32 slides in the protective sleeve 32 under the force of the spring 33 until it moves to the protection position. The needle head of the needle body 35 is located in the protective sleeve 32 again, and the needle head is hidden, which can also reduce accidental injuries caused by waste.

[0107] During the whole process of blood collection, the user will not see the needle body 35, thereby reducing the user's fear and the risk of accidental injury. At the same time, the structure of the blood collection mechanism 3 can also prevent the needle body 35 from being contaminated.

[0108] For example, the needle tip can be a bevel, which is helpful to reduce the contact area with the skin. The diameter of the needle body 35 can be 0.38mm, 0.35mm, 0.32mm. The smaller the diameter, the less pain. The material of the protective cover 32 is preferably silicone.

[0109] like Figure 3 and Figure 4 As shown, specifically, the fixed guide sleeve 31 is provided with a guide groove 311, and the guide groove 311 extends along the sliding direction of the protective sleeve 32. The protective sleeve 32 includes a sleeve head 321, a guide plate 322 and a stopper 323, and the sleeve head 321 is at least partially slidably disposed in the fixed guide sleeve 31, wherein when the protective sleeve 32 is in the protection position, part of the sleeve head 321 is located in the fixed guide sleeve, and the other part extends out of the guide sleeve, thereby shielding the needle head of the needle body 35.

[0110] The spring 33 is disposed between the side wall of the cartridge 1 and the end face of the sleeve 321 , so as to always apply a force away from the side wall to the sleeve 321 , so that the guide sleeve is kept in the protective position.

[0111] Since the distance between the sleeve 321 and the side wall of the cartridge 1 is limited, the distance that the needle protrudes from the sleeve 321 is limited. Also, since the distance that the needle protrudes from the fixed guide sleeve 31 is fixed, the length that the needle penetrates into the finger is limited due to the blocking effect of the sleeve 321 or the fixed guide sleeve 31, thereby preventing the finger from being excessively stabbed.

[0112] The guide plate 322 is connected to the sleeve head 321 and is slidably disposed in the guide groove 311. The stopper 323 is connected to one end of the guide plate 322. The stopper 323 is slidably disposed in the card box 1 and can abut against the inner wall of the card box 1. Specifically, when the protective sleeve 32 is located at the protection position, the stopper 323 can abut against the inner wall of the card box 1, thereby preventing the protective sleeve 32 from being separated from the fixed guide sleeve 31 under the action of the spring 33. The guide plate 322 can also improve the sliding stability of the protective sleeve 32.

[0113] Optionally, the blood sampling mechanism 3 may further include a fixing block 34 , one end of the needle 35 is fixed in the fixing block 34 , the fixing block 34 is fixedly connected to the box body 13 , and further, the fixing block 34 is connected to the bottom wall of the box body 13 .

[0114] like Figure 5 As shown, the quantitative sampler 4 includes a quantitative part 41 and a handheld part 42, wherein the quantitative part 41 is used to absorb a preset amount of whole blood, and the handheld part 42 can be held by the user. The quantitative part 41 includes a whole blood quantitative chamber, which has a relative inlet and outlet and a terminal port. Exemplarily, the whole blood quantitative chamber absorbs whole blood through the action of capillary siphon. The handheld part 42 is connected to the quantitative part 41, and the handheld part is provided with a capillary force release port 422, which is connected to the terminal port. When the whole blood fills the whole blood quantitative chamber, due to the existence of the capillary force release port 422, the capillary force suddenly disappears, and the whole blood stops flowing.

[0115] Optionally, the hand-held portion 42 may also be provided with a plurality of first anti-slip protrusions 421 to prevent the quantitative collector from slipping.

[0116] like Figure 2 As shown, illustratively, a plug hole 132 is provided on the side wall of the card box 1 , and the quantitative portion 41 can be inserted into the plug hole 132 .

[0117] like Figure 2 and Figure 6 As shown, the reagent pack 5 includes a tube body 51 and a sealing film 52, the tube body 51 can hold the reagent, and the sealing film 52 sealing cover is arranged at the tube mouth of the tube body 51. A piercing portion 102 is arranged in the cartridge 1, and illustratively, the piercing portion 102 is connected to the bottom wall of the cartridge body 13. The reagent port 122 is arranged opposite to the piercing portion 102, the tube body 51 can be inserted into the reagent port 122, and the piercing portion 102 can pierce the sealing film 52.

[0118] When the reagent needs to be released, the reagent pack 5 is inserted into the reagent port 122, the reagent pack 5 is pressed, and the piercing portion 102 pierces the sealing film 52, thereby releasing the reagent. The sealing film 52 can not only preserve the reagent, but also make it easy for the reagent pack 5 to be pierced, thereby facilitating the release of the reagent.

[0119] Specifically, the thickness of the sealing film 52 may be 0.05-1 mm. The sealing film 52 may include an aluminum foil and a PE or PET film covering the inner side of the aluminum foil. The PE or PET film may prevent the aluminum foil from reacting with the reagent and may enhance the strength of the sealing film 52 .

[0120] Optionally, the puncture portion 102 is polygonal, and the upper end of the puncture portion 102 is sharp to puncture the sealing film 52. Optionally, the cross-sectional area of ​​the puncture portion 102 gradually increases from top to bottom, so as to facilitate the sealing film 52 to open and release the reagent. Optionally, the cross-sectional area of ​​the puncture portion 102 is rhombus-shaped. The puncture portion 102 can be integrally formed with the box body 13.

[0121] like Figure 2 and Figure 6 As shown, the limiting portion 11 is a hollow structure with two ends open, and the limiting portion 11 is arranged opposite to the reagent port 122, so that the reagent pack 5 is inserted into the limiting portion 11 and the reagent port 122. The limiting portion 11 can also keep the reagent pack 5 stable during the reagent release process, and at the same time, during the transportation process, the limiting portion 11 can also limit the reagent pack 5 to prevent the reagent pack 5 from being lost.

[0122] The reagent pack 5 also includes an elastic positioning portion 53, which can be a rubber ring or a sealing ring. The elastic positioning portion 53 is sleeved outside the tube body 51, and there is a gap between the outer wall of the tube body 51 and the inner wall of the limiting portion 11, and there is an interference fit between the elastic positioning portion 53 and the inner wall of the limiting portion 11. During transportation, a spacing can be provided between the reagent pack 5 and the puncture portion 102, and the elastic positioning portion 53 can prevent the reagent pack 5 from sliding down, thereby preventing the puncture portion 102 from puncturing the reagent pack 5 during transportation. In other optional embodiments, during transportation, the reagent pack 5 can also be inserted upside down in the limiting portion 11, that is, the sealing film 52 is arranged upward and is located outside the limiting portion 11.

[0123] like Figure 7 and Figure 8 As shown, a liquid guide portion 14 is provided on the inner side of the bottom wall of the cartridge 1, and the puncturing portion 102 is provided in the liquid guide portion 14, and the liquid outlet end of the liquid guide portion 14 is adjacent to the liquid inlet end of the liquid channel 2. After the reagent pack 5 is punctured, the reagent directly flows into the liquid guide portion 14, and the reagent continues to flow forward and can directly flow into the liquid inlet end of the liquid channel 2.

[0124] The liquid guide portion 14 is a circular ring structure to reduce the surface tension between the reagent and the liquid guide portion 14 and facilitate the forward flow of the reagent.

[0125] like Figure 2As shown, in this embodiment, the liquid channel 2 also includes a test strip 21, and the antibody and the fluorescent material are arranged on the test strip 21. Exemplarily, it includes a sample pad 211, a fluorescent marker pad 212, a glass cellulose membrane 213 (or a nitrocellulose membrane) and a blotting paper 214 connected in sequence. Exemplarily, the material of the sample pad 211 can be a glass cellulose mold, and the adsorption force of the sample pad 211 on the reagent is greater than the capillary force of the quantitative part 41, so the sample pad 211 can adsorb the sample in the quantitative part 41. The material of the fluorescent marker pad 212 is a nitrocellulose mold, the fluorescent material can be dispersed on the fluorescent marker pad 212, and the antibody can be dispersed on the glass cellulose membrane 213.

[0126] like Figure 7 and Figure 8 As shown, the cartridge 1 may further include a test strip limiting assembly 19 for limiting the test strip 21 , the test strip limiting assembly 19 including a third stop bar 191 and a fourth stop bar 192 , the third stop bar 191 and the fourth stop bar 192 being spaced apart along the width direction of the liquid channel 2 .

[0127] Optionally, the third stop bar 191 and the fourth stop bar 192 are both spaced apart from the side wall of the cartridge 1. The third stop bar 191 and the fourth stop bar 192 both extend along the length direction of the liquid channel 2. The test strip 21 is disposed between the third stop bar 191 and the fourth stop bar 192.

[0128] Furthermore, in order to reduce the residual liquid on the third stop bar 191 and the fourth stop bar 192, the third stop bar 191 and the fourth stop bar 192 can also be connected to the limiting protrusion 193, and the test strip 21 is fixed between the limiting protrusion 193 connected to the third stop bar 191 and the limiting protrusion 193 connected to the fourth stop bar 192. Optionally, the width between the limiting protrusion 193 connected to the third stop bar 191 and the limiting protrusion 193 connected to the fourth stop bar 192 is slightly larger than the width of the test strip 21, and the width difference between the two can be 0.02mm-2mm.

[0129] like Figure 8 As shown, a second bayonet can also be provided in the box body 13, and the quantitative part 41 can be secured in the second bayonet. Specifically, a second bayonet protrusion 103 is connected to the side wall of the card box 1, and a third bayonet protrusion 104 is connected to the third stopper strip 191. The second bayonet protrusion 103 and the third bayonet protrusion 104 are spaced and arranged opposite to each other to form a second bayonet.

[0130] like Figure 6 and Fig. 9As shown, the cartridge 1 further includes a fixing protrusion 15, which is connected to the top wall of the upper cover 12, and the fixing protrusion 15 can be pressed against the upper side of the test strip 21, thereby further fixing the test strip 21. Optionally, the fixing protrusion 15 fixes the absorbent paper 214 of the test strip 21, and the shape of the fixing protrusion 15 is not limited to a cylinder, and can also be a cube, a cuboid, a prism, a cone, etc.

[0131] A guide groove 125 is also provided on the top wall of the upper cover 12. The sample adding port 123 and the guide groove 125 are connected and arranged in sequence along the liquid flow direction, that is, the guide groove 125 is located downstream of the sample adding port 123, so that after the sample is added, the guide groove 125 can guide the sample downstream. Exemplarily, the extension direction of the guide groove 125 is consistent with the length direction of the liquid channel 2.

[0132] A first horizontal column 16 is also connected to the upper cover 12. The first horizontal column 16 is located between the upstream of the observation window 124 and the downstream of the fluorescent material. The first horizontal column 16 is used to slow down the flow rate of the liquid on the test strip 21 so that the fluorescent material can be fully mixed with the liquid (a mixture of reagents and samples) and is conducive to the full reaction of antigens and antibodies.

[0133] The test card provided in this embodiment can be suitable for home self-examination and use in community hospitals, does not require professional medical personnel, and can quantify trace amounts of blood.

[0134] Embodiment 2

[0135] like Figure 10-Figure 18 As shown, the detection card of the second embodiment is basically the same as the above-mentioned first embodiment, and the difference between the two is that the specific structures of the liquid channel 2 and the upper cover 12 are different.

[0136] like Fig.10 As shown, a second anti-slip protrusion 127 may be provided on the top surface of the upper cover 12 to prevent the detection card from slipping out of the hand.

[0137] like Fig.11 As shown, the card box 1 also includes a first stop bar 17 and a second stop bar 18, and the first stop bar 17 and the second stop bar 18 are spaced apart along the width direction of the liquid channel 2, and the first stop bar 17 and the second stop bar 18 both extend along the length direction of the liquid channel 2. At this time, the detection card does not include the third stop bar 191 and the fourth stop bar 192, and the liquid channel 2 is formed between the first stop bar 17 and the second stop bar 18.

[0138] One end of the first stopper strip 17 is connected to one end of the liquid guide portion 14, and one end of the second stopper strip 18 is connected to the other end of the liquid guide portion 14, so that the liquid guide portion 14 is connected to the liquid channel 2. The first stopper strip 17 and the second stopper strip 18 can limit the liquid from flowing out of the liquid channel 2 in the width direction.

[0139] like Fig.12 and Fig.13 As shown, the card box 1 also includes an upper stopper 101 connected to the upper cover 12, and the upper stopper 101 extends along the length direction of the liquid channel 2. The upper stopper 101 is transparent at least at a position opposite to the observation window 124. The upper stopper 101 is provided with an avoidance opening 1011, and the avoidance opening 1011 is arranged opposite to the sample addition port 123.

[0140] like Fig.14 As shown, the lower surface of the upper stopper 101 abuts against the first stopper bar 17 and the second stopper bar 18, and the upper stopper 101, the first stopper bar 17, the second stopper bar 18 and the bottom of the box body 13 form a liquid channel 2, thereby defining a space for liquid flow, and the upper stopper 101, the first stopper bar 17, the second stopper bar 18 and the bottom of the box body 13 define a tubular through structure with front and rear openings, so that the front end of the liquid channel 2 can be connected to the liquid guide part 14, and the liquid can be discharged when it reaches the rear end. At the same time, the upper stopper 101 can effectively limit the liquid from flowing out of the test card.

[0141] Optionally, the upper stopper 101 is transparent, and the upper stopper 101 is connected to the box body 13 by ultrasonic welding. Of course, the upper stopper 101 and the box body 13 can also be integrally formed. Optionally, the upper cover 12 is transparent, which is conducive to reading the fluorescent signal.

[0142] like Fig.15 As shown, in this embodiment, the liquid channel 2 also includes a filter membrane 22, a fluorescent area 23, a valve area 24 and an antibody coating area 25 arranged on the bottom wall of the box body 13 and arranged in sequence. The filter membrane 22 is arranged opposite to the sample addition port 123 and is used to filter red blood cells. The fluorescent area 23 is used to fix the fluorescent material containing antibodies. Optionally, the fluorescent material is a fluorescent microsphere. The valve area 24 is used to prolong the time for the liquid to flow into the antibody coating area 25. The antibody coating area 25 is arranged opposite to the observation window 124 and is used to fix the antibody. The antibody is a specific antibody for the detection antigen. The liquid passes through the filter membrane 22, the fluorescent area 23, the valve area 24 and the antibody coating area 25 in sequence, and the test results are displayed in the antibody coating area 25. The valve area 24 can slow down the flow rate of the liquid, which is beneficial to the antigen and antibody reaction of the fluorescent area 23.

[0143] The fluorescent area 23, the valve area 24 and the antibody coating area 25 can be integrally formed with the box body 13, and the detection card has a simple structure and is easy to process.

[0144] like Fig.16As shown, optionally, a first backflow prevention area 26 is provided between the filter membrane 22 and the fluorescent area 23, and the height of the first backflow prevention area 26 is higher than the height of the fluorescent area 23, so as to prevent the liquid in the fluorescent area 23 from flowing back to the filter membrane 22. Optionally, the first backflow prevention area 26 is 10-200 μm higher than the fluorescent area 23. Optionally, the first backflow prevention area 26 is integrally formed with the bottom wall of the box body 13.

[0145] A drainage structure 27 is provided between the first anti-backflow area 26 and the filter membrane 22. The drainage structure 27 includes a plurality of continuously connected arc-shaped protrusions, which protrude toward the filter membrane 22 and are conducive to transferring the liquid on the filter membrane 22 to the fluorescent area 23. Optionally, the arc-shaped protrusion is a semicircular protrusion, and the height of the arc-shaped protrusion is 3-30 μm.

[0146] A sawtooth deceleration structure 28 is provided between the first anti-backflow zone 26 and the fluorescent zone 23 , which is helpful to slow down the flow rate of the liquid flowing to the fluorescent zone 23 , thereby slowing down the flow rate of the liquid in the fluorescent zone 23 , and further fully dissolving the fluorescent microspheres.

[0147] The fluorescent area 23 includes a plurality of first protrusions 231 arranged at intervals, so as to increase the surface area for binding with the fluorescent microspheres. Furthermore, the first protrusions 231 are hemispherical structures, and the height of the hemispherical structures can be 2-30 μm, so as to have sufficient surface area for binding with the antibodies in the fluorescent microspheres. Optionally, the fluorescent microspheres containing antibodies are fixed on the first protrusions 231 by drying.

[0148] The valve area 24 includes a plurality of second transverse columns 241, which extend along the width direction of the liquid channel 2, and a plurality of second transverse columns 241 extend along the length direction of the liquid channel 2. Exemplarily, one end of the second transverse column 241 is connected to the first stop bar 17, and the other end is connected to the second stop bar 18. Optionally, the height of the second transverse column 241 is 10-80 μm to ensure sufficient blocking time for the liquid.

[0149] A second backflow prevention zone 201 is provided between the valve zone 24 and the antibody coating zone 25, and the height of the second backflow prevention zone 201 is higher than the height of the valve zone 24. Optionally, the height difference between the second backflow prevention zone 201 and the valve zone 24 is 1-20 μm, and the second backflow prevention zone 201 can block the liquid and prevent the liquid from flowing back to the valve zone 24. Optionally, the cross section of the second backflow prevention zone 201 is a triangle, one corner of the triangle extends into the valve zone 24, and the other two corners of the triangle are respectively connected to the first stop bar 17 and the second stop bar 18. Optionally, the second backflow prevention zone 201 is integrally formed with the box body 13.

[0150] The antibody coating area 25 includes a plurality of second protrusions 251 arranged at intervals. For example, the second protrusions 251 are hemispherical structures, and the height of the second protrusions 251 can be 2-50 μm to ensure the coating effect of the antibody. For example, the antibody can be spotted on the antibody coating area 25, and then incubated at 37°C for 2 hours or at 2-8°C overnight, so that the antibody is fixed on the antibody coating area 25.

[0151] like Fig.18 As shown, optionally, multiple first protrusions 231 are arranged into multiple rows, and the multiple rows of first protrusions 231 are arranged in sequence along the length direction of the liquid channel 2, and two adjacent rows of first protrusions 231 are staggered in the width direction of the liquid channel 2, which is beneficial to slow down the flow of liquid and allow the antigen and antibody to fully combine.

[0152] Multiple second protrusions 251 are arranged in multiple rows, and the multiple rows of second protrusions 251 are arranged in sequence along the length direction of the liquid channel 2. Two adjacent rows of second protrusions 251 are staggered in the width direction of the liquid channel 2, which is beneficial to slow down the flow of liquid and allow the antigen and antibody to fully combine.

[0153] like Fig.15 As shown, optionally, the liquid channel 2 further includes a waste liquid area 29, which is located downstream of the antibody coating area 25, and the height of the waste liquid area 29 is lower than the height of the antibody coating area 25, so as to facilitate the liquid to flow into the waste liquid area 29. Exemplarily, the upper stopper 101 does not extend to the waste liquid area 29 to increase the space of the waste liquid area 29. Optionally, the height difference between the waste liquid area 29 and the antibody coating area 25 is 20μm-200μm.

[0154] The test card provided in this embodiment integrates the functions of whole blood collection, whole blood quantification, sample dilution, and antigen detection in one test card, which is suitable for families and community hospitals. The test card reduces the possibility of sample contamination, improves the accuracy of detection, can effectively collect blood, and reduce the user's pain and fear.

[0155] Although the utility model has been described in detail above by means of general description, specific implementation methods and tests, it is obvious to those skilled in the art that some modifications or improvements can be made to the utility model. Therefore, these modifications or improvements made without departing from the spirit of the utility model are within the scope of protection claimed by the utility model.

Claims

1. A detection card, characterized in that: include: A cartridge (1), wherein a sample addition port (123) and an observation window (124) are provided on a top wall of the cartridge (1), a liquid channel (2) is provided inside the cartridge (1), the liquid channel (2) comprises an antibody arranged at a position directly opposite to the observation window (124), and a fluorescent material located upstream of the antibody, the sample addition port (123) and the observation window (124) being arranged in sequence along the length direction of the liquid channel (2); A blood sampling mechanism (3) is arranged on a side wall of the cartridge (1), the blood sampling mechanism (3) comprising a needle body (35) capable of puncturing a position to be blood sampled, and the blood sampling mechanism (3) is capable of switching between a working state in which the needle head of the needle body (35) is exposed and a protection state in which the needle body (35) is hidden; The quantitative sampler (4) is detachably connected to the side wall of the cartridge (1) and is capable of collecting a preset amount of sample. The sample in the quantitative sampler (4) can enter the liquid channel (2) through the sample addition port (123).

2. The detection card according to claim 1, characterized in that: The blood sampling mechanism (3) comprises: A fixed guide sleeve (31) connected to the side wall of the card box (1); A protective sleeve (32) is at least partially slidably disposed in the fixed guide sleeve (31), the protective sleeve (32) can be located in a protection position and a working position by sliding along the fixed guide sleeve (31), the needle body (35) is disposed in the protective sleeve (32), the end of the needle body (35) is fixed to the cartridge (1), the needle head extends out of the fixed guide sleeve (31), the protective sleeve (32) is located in the working position, the needle head extends out of the protective sleeve (32), so that the blood sampling mechanism (3) is in the working state, the protective sleeve (32) is located in the protection position, the needle head is located in the protective sleeve (32), so that the blood sampling mechanism (3) is in the protection state; A spring (33) is arranged in the fixed guide sleeve (31), and the spring (33) can drive the protective sleeve (32) to be located at the protective position.

3. The detection card according to claim 2, characterized in that: The fixed guide sleeve (31) is provided with a guide groove (311), and the guide groove (311) extends along the sliding direction of the protective sleeve (32); The protective cover (32) comprises: A sleeve (321) is at least partially slidably disposed in the fixed guide sleeve (31), and the spring (33) is disposed between the side wall of the cartridge (1) and the end surface of the sleeve (321); A guide plate (322) connected to the sleeve (321) and slidably disposed in the guide groove (311); A stopper (323) is connected to one end of the guide plate (322); the stopper (323) is slidably disposed in the card box (1) and is capable of abutting against the inner wall of the card box (1).

4. The detection card according to claim 1, characterized in that: The quantitative sampler (4) comprises: A quantitative part (41), the quantitative part (41) comprising a whole blood quantitative chamber, the whole blood quantitative chamber having an inlet and an outlet opposite to each other and a terminal port; The hand-held part (42) is connected to the quantitative part (41), and the hand-held part (42) is provided with a capillary force release port (422), and the capillary force release port (422) is communicated with the terminal port.

5. The detection card according to claim 4, characterized in that: The side wall of the card box (1) is provided with an insertion hole (132), and the quantitative portion (41) can be inserted into the insertion hole (132).

6. The detection card according to claim 1, characterized in that: The top wall of the cartridge (1) is also provided with a reagent port (122), and the reagent port (122), the sample addition port (123) and the observation window (124) are sequentially arranged along the length direction of the liquid channel (2); The detection card further comprises a reagent pack (5); the top surface of the card box (1) is connected to a limiting portion (11); the reagent pack (5) can be arranged on the limiting portion (11); and the reagent in the reagent pack (5) can enter the liquid channel (2) through the reagent port (122).

7. The detection card according to claim 6, characterized in that: The reagent kit (5) comprises: A tube body (51), wherein the tube body (51) is capable of containing a reagent; A sealing film (52), wherein the sealing film (52) is sealed and covered at the tube opening of the tube body (51); The cartridge (1) is provided with a piercing portion (102), the reagent port (122) is arranged opposite to the piercing portion (102), the tube (51) can be inserted into the reagent port (122), and the piercing portion (102) can pierce the sealing film (52).

8. The detection card according to claim 7, characterized in that: The limiting portion (11) is a hollow structure with openings at both ends. The limiting portion (11) and the reagent port (122) are arranged opposite to each other so that the reagent pack (5) can be inserted into the limiting portion (11) and the reagent port (122).

9. The detection card according to claim 8, characterized in that: The reagent pack (5) further comprises an elastic positioning portion (53), wherein the elastic positioning portion (53) is sleeved outside the tube body (51), and a gap is provided between the outer wall of the tube body (51) and the inner wall of the limiting portion (11), and the elastic positioning portion (53) and the inner wall of the limiting portion (11) are interference fit.

10. The detection card according to claim 7, characterized in that: A liquid guide portion (14) is provided on the inner side of the bottom wall of the cartridge (1), the piercing portion (102) is provided in the liquid guide portion (14), and the liquid outlet end of the liquid guide portion (14) is adjacent to the liquid inlet end of the liquid channel (2).

11. The test card according to any one of claims 1 to 10, characterized in that: The card box (1) comprises: A box body (13), wherein the upper end of the box body (13) is open; The upper cover (12) is arranged to cover the opening of the box body (13); the sample addition port (123) and the observation window (124) are both provided on the upper cover (12); and the liquid channel (2) is located between the box body (13) and the upper cover (12).

12. The test card according to claim 11, characterized in that: The liquid channel (2) further comprises a test strip (21), and the antibody and the fluorescent material are arranged on the test strip (21).

13. The detection card according to claim 12, characterized in that: The card box (1) further comprises a fixing protrusion (15), wherein the fixing protrusion (15) is connected to the top wall of the upper cover (12), and the fixing protrusion (15) can be pressed against the upper side of the test strip (21).

14. The detection card according to claim 12, characterized in that: The top wall of the upper cover (12) is also provided with a guide groove (125), the sample addition port (123) and the guide groove (125) are connected and are arranged in sequence along the flow direction of the liquid, and / or The upper cover (12) is also connected to a first transverse column (16), the first transverse column (16) being located between the upstream of the observation window (124) and the downstream of the fluorescent material, the first transverse column (16) being used to slow down the flow rate of the liquid on the test strip (21).

15. The detection card according to claim 11, characterized in that: The liquid channel (2) further comprises a filter membrane (22), a fluorescent area (23), a valve area (24) and an antibody coating area (25) which are arranged on the bottom wall of the box body (13) and arranged in sequence; The filter membrane (22) is arranged opposite to the sample injection port (123) and is used to filter red blood cells. The fluorescent area (23) is used to fix the fluorescent material containing antibodies. The valve area (24) is used to prolong the time for liquid to flow into the antibody coating area (25). The antibody coating area (25) is arranged opposite to the observation window (124) and is used to fix the antibodies.

16. The test card according to claim 15, characterized in that: The valve area (24) comprises a plurality of second transverse columns (241), wherein the second transverse columns (241) extend along the width direction of the liquid channel (2), and the plurality of second transverse columns (241) extend along the length direction of the liquid channel (2); and / or The fluorescent area (23) comprises a plurality of first protrusions (231) arranged at intervals; and / or The antibody coating area (25) includes a plurality of second protrusions (251) arranged at intervals.

17. The test card according to claim 16, characterized in that: A plurality of the first protrusions (231) are arranged in a plurality of rows, the plurality of rows of the first protrusions (231) are arranged in sequence along the length direction of the liquid channel (2), and two adjacent rows of the first protrusions (231) are arranged in a staggered manner in the width direction of the liquid channel (2); and / or A plurality of the second protrusions (251) are arranged in a plurality of rows. The plurality of rows of the second protrusions (251) are arranged in sequence along the length direction of the liquid channel (2). Two adjacent rows of the second protrusions (251) are arranged in a staggered manner in the width direction of the liquid channel (2).

18. The test card according to claim 16, characterized in that: The first protrusion (231) and / or the second protrusion (251) are hemispherical structures; and / or The height of the first protrusion (231) and / or the second protrusion (251) is 2-50 μm; and / or The height of the second cross column (241) is 10-80 μm.

19. The test card according to claim 15, characterized in that: A first backflow prevention zone (26) is provided between the filter membrane (22) and the fluorescent zone (23), and the height of the first backflow prevention zone (26) is higher than the height of the fluorescent zone (23); and / or A second anti-backflow zone (201) is arranged between the valve zone (24) and the antibody coating zone (25), and the height of the second anti-backflow zone (201) is higher than the height of the valve zone (24).

20. The test card according to claim 19, characterized in that: A drainage structure (27) is provided between the first anti-backflow zone (26) and the filter membrane (22), the drainage structure (27) comprising a plurality of continuously connected arc-shaped protrusions, the arc-shaped protrusions protruding toward the filter membrane (22); and / or A sawtooth-shaped deceleration structure (28) is provided between the first anti-backflow zone (26) and the fluorescent zone (23).

21. The test card according to claim 15, characterized in that: The liquid channel (2) further comprises a waste liquid area (29), wherein the waste liquid area (29) is located downstream of the antibody coating area (25), and the height of the waste liquid area (29) is lower than the height of the antibody coating area (25).

22. The test card according to claim 15, characterized in that: The fluorescent area (23), the valve area (24) and the antibody coating area (25) are integrally formed with the box body (13).

23. The test card according to claim 15, characterized in that: The card box (1) further comprises a first stop bar (17) and a second stop bar (18), wherein the first stop bar (17) and the second stop bar (18) are arranged at intervals along the width direction of the liquid channel (2), and the first stop bar (17) and the second stop bar (18) both extend along the length direction of the liquid channel (2); The liquid channel (2) is formed between the first stopper strip (17) and the second stopper strip (18).

24. The test card according to claim 23, characterized in that: The card box (1) also includes an upper stopper (101) connected to the upper cover (12); the upper stopper (101) is transparent at least at a position opposite to the observation window (124); the upper stopper (101) is provided with an escape opening (1011); the escape opening (1011) is arranged opposite to the sample injection port (123); the lower surface of the upper stopper (101) is in contact with the first stopper strip (17) and the second stopper strip (18); the upper stopper (101), the first stopper strip (17), the second stopper strip (18) and the bottom of the box body (13) form the liquid channel (2).

Images