Reagent cleaning and mixing device and immunohistochemical instrument

Abstract

The utility model relates to a reagent cleaning and mixing device and an immunohistochemical instrument, and relates to the technical field of biological sample treatment equipment.The reagent cleaning and mixing device comprises a device body, a cleaning assembly and a mixing assembly, and the cleaning assembly and the mixing assembly are both arranged on the device body; the cleaning assembly comprises a plurality of cleaning holes for cleaning the sampling needle, and the cleaning holes are formed in the upper side of the device body; the mixing assembly comprises a cup holder, a plurality of mixing cups are arranged on the cup holder, and the cup holder is arranged on the device body. The cleaning assembly and the mixing assembly are integrated on the device body, and the sampling needle can be quickly moved into the corresponding cleaning hole to be cleaned after the sampling needle sucks a corresponding reagent or sample and adds the reagent or sample into the mixing cup, so that on one hand, the transfer time of the sampling needle can be shortened, and the sampling efficiency is improved; further, residual reagents on the sampling needle can be cleaned more conveniently; and on the other hand, the worker does not need to frequently move, so that the working intensity of the worker is reduced.

Description

Technical Field

[0001] The present application relates to the technical field of biological sample processing equipment, and in particular to a reagent cleaning and mixing device and an immunohistochemical instrument. Background Art

[0002] Immunohistochemistry analyzer is an analytical instrument used in the fields of biology, basic medicine, and clinical medicine. It is mainly used for immunohistochemical staining to detect specific antigens or antibodies in cells or tissues.

[0003] When processing biological samples, immunohistochemistry instruments need to use multiple reagents or mix the sample with the corresponding reagents. In the above process, the two most commonly used devices are sampling needles and mixing cups. The corresponding reagents or samples are sucked by the sampling needle, and the corresponding reagents or samples are added to the mixing cup. Then the sampling needle is cleaned, and the above steps are repeated. All reagents or samples are added to the mixing cup in turn, and then the reagents in the mixing cup are mixed to make the final reagent or biological sample.

[0004] However, in order to ensure that the sampling needle does not contaminate the absorbed reagent or sample each time a reagent or sample is added, the sampling needle should be cleaned before and after each sampling. This results in the need to constantly move back and forth between the cleaning tank and the mixing cup during work, which reduces work efficiency. Utility Model Content

[0005] The present application provides a reagent cleaning and mixing device and an immunohistochemical instrument, the purpose of which is to fix the mixing cup and clean the sampling needle at the same time through a device, and the structure is reasonable and the working distance is short, which can effectively improve the work efficiency.

[0006] The present application provides a reagent cleaning and mixing device that adopts the following technical solution:

[0007] A reagent cleaning and mixing device comprises a device body, a cleaning component and a mixing component, wherein the cleaning component and the mixing component are both arranged on the device body; the cleaning component comprises a plurality of cleaning holes for cleaning sampling needles, wherein the cleaning holes are all arranged on the upper side of the device body; the mixing component comprises a cup holder, wherein a plurality of mixing cups are arranged on the cup holder, and the cup holder is arranged on the device body.

[0008] By adopting the above technical solution, a cleaning component is arranged on the device body, and the cleaning component includes a cleaning hole, so that the sampling needle can be moved to the corresponding cleaning hole, and the cleaning liquid is injected into the cleaning hole to directly clean the sampling needle. The setting of the mixing component can be used to provide a mixing cup.

[0009] Therefore, each time after the corresponding reagent or sample is sucked up by the sampling needle and added to the mixing cup, the sampling needle can be quickly moved to the corresponding cleaning hole for cleaning. On the one hand, the transfer time of the sampling needle can be reduced, making it easier to clean the residual reagent on the sampling needle; on the other hand, when preparing biological samples, the staff no longer needs to move frequently, which reduces the staff's workload and increases the speed of biological sample preparation.

[0010] Optionally, a spray component is also included, and the cleaning component, mixing component and spray component are all arranged on the device body, and the cleaning component and spray component are arranged adjacent to each other.

[0011] By adopting the above technical solution, in the process of preparing the biological sample, the required reagents can be mixed in the mixing cup through the mixing component, the sampling needle can be cleaned through the cleaning component, and the glass slide can be cleaned through the spray component. Non-toxic waste liquid is used in the cleaning component and the spray component, while toxic solvent may exist in the mixing cup in the mixing component, so the spray component and the cleaning component are arranged adjacent to each other, so that the two can share a water outlet and the water outlet of the mixing component is separated from the two, thereby simplifying the structure.

[0012] Optionally, the cleaning component includes a cleaning pool, the bottom of the cleaning pool is provided with a No. 1 hole for cleaning the inner wall of the sampling needle, the side wall of the No. 1 hole is provided with a waste liquid hole for collecting waste liquid, and the waste liquid hole runs through the device body.

[0013] By adopting the above technical solution, the setting of the cleaning pool facilitates the collection of cleaning liquid in the cleaning pool to prevent the cleaning liquid from overflowing the device body. A No. 1 hole is opened at the bottom of the cleaning pool, so before the sampling needle is sent into the plurality of cleaning holes for cleaning, the inner wall of the sampling needle can be cleaned after the sampling needle is sent into the No. 1 hole. The specific cleaning method is: the cleaning liquid is drawn into the corresponding syringe by the injection pump, and then the liquid is discharged by the injection pump so that the cleaning liquid can flush the inner wall of the sampling needle.

[0014] At the same time, a drainage pump can be installed on the device body, and the waste liquid hole can be connected through the drainage pump. Then, during the cleaning process of the inner wall of the sampling needle and after the cleaning is completed, the drainage pump can extract the cleaning liquid flowing out of the sampling needle.

[0015] Therefore, hole No. 1 is mainly used to collect the waste liquid flowing out from cleaning the inner wall of the sampling needle. The cleaning of the inner wall of the sampling needle is actually achieved by pumping out the cleaning liquid through the injection pump.

[0016] Optionally, the spray assembly includes a spray pool, a negative pressure suction hole is opened at the bottom of the spray pool, and the negative pressure suction hole is connected to the waste liquid hole.

[0017] By adopting the above technical solution, the glass slide will be cleaned by the spray head during the preparation process of the biological sample. Before the spray head is used normally, there is some air in the spray head. Directly using the spray head to discharge the spray liquid will make the spray liquid spray unsmooth or uneven. Therefore, when using the spray head, pre-spray in the spray pool first, so that the air in the spray head can be discharged, thereby ensuring the normal spraying of the spray head. The negative pressure suction hole can accelerate the discharge of the spray waste liquid in the spray pool.

[0018] Optionally, the cleaning component, mixing component and spray component are arranged in a straight line.

[0019] By adopting the above technical solution, in the process of preparing biological samples, the mixing component can be used to mix the required reagents in the mixing cup, the cleaning component can be used to clean the sampling needle, and the spray component can be used to clean the glass slide. When the mixing component, the cleaning component and the spray component are arranged in a straight line, the sampling needle is automatically moved between the mixing component, the cleaning component and the spray component by an automated machine, thereby facilitating the full automation of the machine, that is, in the process of preparing biological samples, the mixing, cleaning and spraying operations can be fully automatically realized.

[0020] Optionally, a cleaning pool is provided on the upper side of the device body, a cleaning platform is provided on the inner wall of the cleaning pool, and a plurality of cleaning holes are provided on the upper side of the cleaning platform and are arranged in a straight line.

[0021] By adopting the above technical solution, the setting of the cleaning pool facilitates the collection of cleaning liquid in the cleaning pool, preventing the cleaning liquid from overflowing the device body. A cleaning platform is set in the cleaning pool, and the cleaning holes are all opened on the cleaning platform, so it can prevent the used cleaning liquid in the cleaning pool from flowing back into the cleaning holes, thereby contaminating the cleaning holes. Since the inner wall, needle tip and outer wall of the sampling needle need to be cleaned when cleaning the sampling needle, multiple cleaning holes need to be set, and when the multiple cleaning holes are arranged in a straight line, it is convenient for the automated machine to automatically move the sampling needle between several cleaning holes, so it is convenient for the automated cleaning of the sampling needle.

[0022] Optionally, a position calibration point is provided on the device body, and the position calibration point is located on the straight line where the cleaning hole is located.

[0023] By adopting the above technical solution and setting the position calibration point, when the fully automated machine is installed and used for the first time or after maintenance and replacement, the fully automated machine can adjust and confirm the position of the sampling needle through the position calibration point when re-grasping the sampling needle. Since the position calibration point and the plurality of cleaning holes are arranged in a straight line, after aligning the calibration point, the position coordinates of the plurality of cleaning holes are automatically adjusted according to the distance relationship set by the program, simplifying the calibration process.

[0024] Optionally, the plurality of mixing cups are located on the same straight line, and the straight line where the plurality of mixing cups are located coincides with the straight line where the plurality of cleaning holes are located.

[0025] By adopting the above technical solution, the mixing cup is also arranged in a straight line with the position calibration point and the plurality of cleaning holes, so after the automated machine aligns the sampling needle with the position calibration point, the position coordinates of the mixing cup can be automatically adjusted, thereby simplifying the calibration process.

[0026] Optionally, a metal shielding plate is provided on the outer side of the device body.

[0027] By adopting the above technical solution, since cleaning and mixing need to be achieved during the automated operation of the machine, liquid level detection is inevitably required, and liquid level detection mostly uses capacitive liquid level sensors. Therefore, the setting of the metal shielding plate can achieve grounding, and then cooperate with the corresponding liquid level sensor to realize the liquid level detection function.

[0028] On the other hand, the present application also provides an immunohistochemistry instrument using the following technical solution:

[0029] An immunohistochemical instrument comprises: the above-mentioned reagent cleaning and mixing device.

[0030] By adopting the above technical solution and using the above reagent cleaning and mixing device in an immunohistochemical instrument, automatic cleaning of the sampling needle, automatic mixing of reagents and automatic spray cleaning can be achieved.

[0031] In summary, the present application includes at least one of the following beneficial technical effects:

[0032] 1. The present application integrates the cleaning component and the mixing component into the device body, so that the sampling needle can be cleaned in time after each sampling is completed, which can increase work efficiency.

[0033] 2. Since the cleaning component and the mixing component are integrated into the device body, the time that the reagent or sample remains in the sampling needle can be reduced, making it easier to clean the residual reagent on the sampling needle.

[0034] 3. Through the setting of the spray component, the present application can realize the pre-spraying of the cleaning liquid in the spray pool to exhaust the air in the pipe, thereby ensuring that the spray liquid can flow out normally during use and ensuring the normal progress of the experiment.

[0035] 4. In the present application, several mixing cups, cleaning holes and position calibration points are all located on the same straight line, so the difficulty of positioning and calibrating the sampling needle in the immunohistochemical instrument can be simplified. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 It is a schematic diagram of the overall structure of the reagent cleaning and mixing device of the present application.

[0037] Figure 2 It is a schematic cross-sectional structure diagram of the reagent cleaning and mixing device of the present application.

[0038] Figure 3 It is a schematic diagram of the explosion structure of the reagent cleaning and mixing device of the present application.

[0039] In the figure, 1, device body; 11, metal shielding plate; 12, position calibration point;

[0040] 2. Cleaning assembly; 21. Cleaning tank; 22. Cleaning platform; 23. Cleaning hole; 231. No. 2 hole; 232. No. 3 hole; 233. No. 4 hole; 234. Drain trough; 24. No. 1 hole; 25. Waste liquid hole; 26. Overflow platform; 261. No. 1 trough; 262. No. 2 trough; 263. No. 1 hole;

[0041] 3. Mixing assembly; 31. Mixing tank; 311. Mounting slot; 312. Give way slot; 313. Middle slot; 314. Long drain slot; 315. Positioning slot; 316. Pick-up slot; 32. Cup holder; 321. Overflow slot; 322. Overflow hole; 323. Overflow pipe; 324. Positioning block; 325. Pick-up plate; 33. Mixing cup;

[0042] 4. Spray assembly; 41. Spray pool; 42. Negative pressure suction hole. DETAILED DESCRIPTION

[0043] The following is combined with Figure 1 -Attached Figure 3 , further details of this application are given.

[0044] A reagent cleaning and mixing device, referring to Figure 1 , including a device body 1, a mixing component 3 and a cleaning component 2 are arranged on the upper side of the device body 1, the mixing component 3 includes a cup holder 32, and a plurality of mixing cups 33 are arranged on the cup holder 32; the cleaning component 2 includes a plurality of cleaning holes 23, and the plurality of cleaning holes 23 are opened on the upper side of the device body 1.

[0045] Therefore, the mixing assembly 3 is configured to provide a mixing cup 33, and the opening of the cleaning hole 23 enables the sampling needle to be moved into the corresponding cleaning hole 23, and the cleaning liquid is injected into the cleaning hole 23, so that the sampling needle can be directly cleaned.

[0046] Reference Figure 1 and Figure 2 The cleaning component 2 includes a cleaning pool 21 , which is opened on the upper side of the device body 1 . A cleaning platform 22 is provided on an inner side wall of the cleaning pool 21 along the width direction of the device body 1 , and a plurality of cleaning holes 23 are opened on the cleaning platform 22 .

[0047] In the present application, the arrangement of the plurality of cleaning holes 23 is designed according to actual conditions.

[0048] Preferably, the cleaning holes 23 are arranged in sequence and at intervals along the length direction of the device body 1, that is, the cleaning holes 23 are arranged in a straight line. The cleaning holes 23 arranged in a straight line are conducive to the positioning and rapid positioning of the automated machine.

[0049] In addition, the plurality of cleaning holes 23 can also be arranged in a staggered manner. When the position of each cleaning hole 23 is fixed, the relative coordinates between the plurality of cleaning holes 23 are clear, which can also meet the above requirements.

[0050] In addition, in the present application, the number, inner diameter and depth of the cleaning holes 23 are designed according to the sampling needles that need to be cleaned.

[0051] Specifically, since the sampling needle to be cleaned in this application needs to clean the inner cavity of the sampling needle, the needle tip of the sampling needle and the outer wall of the sampling needle, in addition, a liquid level detection hole needs to be set. Therefore, the number of cleaning holes 23 in this application is at least three. Preferably, in this embodiment, refer to Figure 1 and Figure 2 The cleaning holes 23 include a No. 2 hole 231, a No. 3 hole 232 and a No. 4 hole 233. The No. 2 hole 231, the No. 3 hole 232 and the No. 4 hole 233 are all opened in the vertical direction and are arranged in sequence along the length direction of the device body 1. Among the No. 2 hole 231, the No. 3 hole 232 and the No. 4 hole 233, the No. 4 hole 233 has the deepest depth and is arranged according to the sampling needle that needs to be cleaned. The depths of the No. 2 hole 231 and the No. 3 hole 232 are different.

[0052] Therefore, the No. 2 hole 231 is used for liquid level detection, the No. 3 hole 232 is used for cleaning the tip of the sampling needle, and the No. 4 hole 233 is used for cleaning the outer wall of the sampling needle.

[0053] Reference Figure 2 and Figure 3 A hole No. 24 is also provided at the bottom of the cleaning pool 21. The hole No. 24 is provided in the vertical direction. A waste liquid hole 25 is provided at the bottom of the hole No. 24. The waste liquid hole 25 is provided along the length direction of the device body 1. One end of the waste liquid hole 25 in the length direction is connected to the hole No. 24, and the other end passes through the side wall of the device body 1.

[0054] Reference Figure 2 and Figure 3When cleaning the sampling needle, the cleaning liquid is drawn into the corresponding syringe by the injection pump, and then the liquid is discharged by the injection pump. In this way, the inner wall of the sampling needle in the No. 1 hole 24 is cleaned. A drainage pump can be installed on the device body 1, and the waste liquid hole 25 can be connected through the drainage pump. Then, during the cleaning process of the inner wall of the sampling needle and after the cleaning is completed, the drainage pump can extract the cleaning liquid flowing out of the sampling needle. Therefore, the inner wall of the sampling needle can be cleaned by pumping the cleaning liquid by the injection pump.

[0055] After that, after the No. 1 hole 24 is cleaned, the cleaning liquid is pumped out by the syringe pump, and the cleaning liquid is discharged to the No. 3 hole 232 through the sampling needle in a high-pressure state, so that the tip of the sampling needle can be cleaned in the No. 3 hole 232. Similarly, after the No. 3 hole 232 is cleaned, the cleaning liquid is pumped out by the syringe pump, and the cleaning liquid is discharged to the No. 4 hole 233 through the sampling needle in a high-pressure state, so that the outer wall of the sampling needle can be cleaned in the No. 4 hole 233. The No. 2 hole 231 is used to detect the liquid level of the cleaning liquid.

[0056] And because the No. 1 hole 24 is opened at the bottom of the cleaning tank 21, the No. 1 hole 24 can also collect and discharge all the cleaning waste liquid.

[0057] Preferably, in this embodiment, refer to Figure 2 and Figure 3 Hole No. 24 is located on a straight line where the cleaning holes 23 are located. Hole No. 24 is located on the side of hole No. 231 away from hole No. 3 232 along the length direction of the device body 1. The depth of hole No. 1 24 is less than the depth of hole No. 4 233. The depth of hole No. 1 24 is greater than the depths of hole No. 231 and hole No. 3 232.

[0058] In addition, the No. 1 hole 24 can also be arranged alternately with the plurality of cleaning holes 23, and the relative position relationship between the No. 1 hole 24 and the plurality of cleaning holes 23 is determined, which can also meet the above requirements.

[0059] Reference Figure 1 and Figure 2 , a drainage groove 234 is also provided on the upper side of the cleaning platform 22. The drainage groove 234 is arranged one-to-one with the cleaning hole 23. The length direction of the drainage groove 234 is arranged along the width direction of the device body 1. One side of the drainage groove 234 is connected with the corresponding cleaning hole 23 in the length direction, and the other side passes through the cleaning platform 22. Therefore, the bottom of the drainage groove 234 is lower than the upper side of the cleaning platform 22, and then in the process of cleaning the sampling needle, the excess cleaning liquid will pass through the corresponding drainage groove 234 and be discharged into the cleaning pool 21, and then into the No. 1 hole 24, and finally discharged through the waste liquid hole 25. Therefore, the opening of the drainage groove 234 can, on the one hand, prevent the cleaning water from overflowing the device body 1; on the other hand, it can also prevent the cleaning water in different cleaning holes 23 from contaminating each other.

[0060] Reference Figure 1 and Figure 2 A position calibration point 12 is arranged on the upper side of the device body 1. The arrangement of the position calibration point 12 enables the position calibration of the sampling needle to be achieved through the position calibration point 12 when the automated machine uses the sampling needle for automated cleaning.

[0061] Reference Figure 1 and Figure 2 The position calibration point 12 and the hole No. 1 24 , the hole No. 231 , the hole No. 3 232 and the hole No. 4 233 are all located on the same straight line, and the spacing direction of the position calibration point 12 and the hole No. 1 24 , the hole No. 231 , the hole No. 3 232 and the hole No. 4 233 is set along the spacing direction of the cleaning component 2 and the mixing component 3.

[0062] Therefore, when the fully automated machine is installed and used for the first time or after maintenance and replacement, the fully automated machine adjusts and confirms the position of the sampling needle through the position calibration point 12. Since the position calibration point 12 and the No. 1 hole 24, the No. 2 hole 231, the No. 3 hole 232 and the No. 4 hole 233 are arranged in a straight line, after the position calibration point 12, the position coordinates of the No. 1 hole 24, the No. 2 hole 231, the No. 3 hole 232 and the No. 4 hole 233 are automatically adjusted according to the distance relationship set by the program, thereby simplifying the calibration process.

[0063] Reference Figure 2 and Figure 3 The mixing assembly 3 further includes a mixing tank 31, a mounting groove 311 is provided at the bottom of the mixing tank 31, a cup holder 32 is plugged and matched with the mounting groove 311 in the vertical direction, and the cup holder 32 slides along the inner wall of the mounting groove 311 in the vertical direction, and the bottom of the cup holder 32 abuts against the bottom of the mounting groove 311. Therefore, the mounting groove 311 is provided for mounting the cup holder 32.

[0064] Reference Figure 2 and Figure 3 The bottom of the installation groove 311 is provided with a clearance groove 312, and the mixing cup 33 is plugged and matched with the clearance groove 312 along the vertical direction, and the mixing cup 33 slides along the inner wall of the clearance groove 312 along the vertical direction. Therefore, the clearance groove 312 is provided to provide a clearance space for the mixing cup 33.

[0065] Reference Figure 2 and Figure 3The length direction of the cup holder 32 is arranged along the length direction of the device body 1, and a plurality of mixing cups 33 are arranged in sequence along the length direction of the cup holder 32. The cup holder 32 and the mixing cup 33 are detachably connected. An overflow groove 321 is provided on the upper side of the cup holder 32. The mixing cup 33 is arranged along the vertical direction, and the upper side of the mixing cup 33 is higher than the bottom of the overflow groove 321. Therefore, the mixing cup 33 is used to achieve the mixing of reagents, and the overflow groove 321 is provided to collect the reagents overflowing from the mixing cup 33 to prevent the reagents from overflowing out of the device body 1.

[0066] Reference Figure 2 and Figure 3 The bottom of the installation groove 311 is provided with an intermediate groove 313, which is connected to the clearance groove 312. The bottom of the overflow groove 321 is provided with an overflow hole 322, which is connected to the intermediate groove 313. The bottom of the clearance groove 312 is provided with a drainage groove 314, the length direction of which is arranged along the length direction of the device body 1, the drainage groove 314 is connected to the clearance groove 312, and one side of the length of the drainage groove 314 passes through the side wall of the device body 1.

[0067] Therefore, the reagent overflowing from the mixing cup 33 can enter the drainage groove 234 through the overflow hole 322 , and then be discharged into the drainage long groove 314 , and then flow out through the drainage long groove 314 .

[0068] Reference Figure 2 and Figure 3 An overflow platform 26 is provided on the inner wall of the cleaning tank 21 near the mixing tank 31, and a first groove 261 is provided on the upper side of the overflow platform 26. The length direction of the first groove 261 is arranged along the width direction of the device body 1, and a second groove 262 is provided on the inner wall of the first groove 261 away from the mixing tank 31 along its own width direction. The second groove 262 penetrates the side wall corresponding to the first groove 261 along the length direction of the device body 1, and the second groove 262 penetrates the upper side of the overflow platform 26. Therefore, the first groove 261 and the second groove 262 are connected to the cleaning tank 21.

[0069] Reference Figure 2 and Figure 3 The bottom of the first groove 261 is also provided with a first hole 263, which vertically penetrates the bottom of the first groove 261, and the side wall of the first hole 263 is connected to one side of the length direction of the drainage groove 314. Therefore, the drainage groove 314 can also discharge the overflowed reagent into the first hole 263, and then discharge it from the device body 1 through the first hole 263.

[0070] Reference Figure 2 and Figure 3, the bottom of the first groove 261 is flush with the bottom of the second groove 262, the bottom of the first groove 261 is higher than the bottom of the drainage groove 234 and the bottom of the first groove 261 is also higher than the bottom of the overflow groove 321. Therefore, the liquid that cannot be discharged from the cleaning tank 21 can also flow from the second groove 262 into the first groove 261, and then be discharged from the first hole 263; similarly, when the waste liquid that cannot be discharged from the first hole 24, that is, when the waste liquid hole 25 or the pump connected to the waste liquid hole 25 fails, the waste liquid will also overflow into the first groove 261 and the second groove 262, and finally be discharged from the first hole 263, so as to prevent the waste liquid from overflowing the cleaning tank 21.

[0071] Reference Figure 2 and Figure 3 The overflow trough 321 is provided with an overflow pipe 323 at its bottom, which is arranged in a vertical direction, and the lower end of the overflow pipe 323 is connected to the bottom of the overflow trough 321, and the overflow pipe 323 is connected to the overflow hole 322. Therefore, the overflow pipe 323 can be arranged so that after a certain amount of reagent is stored in the overflow trough 321, the excess reagent will be discharged from the overflow pipe 323.

[0072] Reference Figure 2 and Figure 3 The bottom of the installation groove 311 is provided with a positioning groove 315, and the positioning groove 315 is connected with the clearance groove 312. A positioning block 324 is provided at the lower side of the cup holder 32, and the positioning block 324 is plugged and matched with the positioning groove 315, and the positioning block 324 is plugged and matched with the positioning groove 315 along the vertical direction. Therefore, the arrangement of the positioning block 324 and the positioning groove 315 can improve the positioning accuracy of the installation of the cup holder 32.

[0073] Reference Figure 2 and Figure 3 , a pick-up plate 325 is provided on the cup holder 32, and the pick-up plate 325 is located on the side of the cup holder 32 in the length direction away from the cleaning tank 21. A pick-up groove 316 is provided on the upper side of the device body 1, and the pick-up groove 316 is located on the side of the mixing tank 31 away from the cleaning tank 21 along the length direction of the device body 1. The pick-up groove 316 is connected to the mixing tank 31 on one side along the length direction of the device body 1, and the other side penetrates the device body 1. The pick-up plate 325 is engaged with the pick-up groove 316, and the pick-up plate 325 is connected to the cup holder 32 on one side along the length direction of the device body 1, and the other side extends beyond the corresponding side wall of the device body 1. Therefore, the cooperation between the pick-up plate 325 and the pick-up groove 316 facilitates the positioning and installation of the cup holder 32, and the pick-up plate 325 also facilitates the staff to take the cup holder 32.

[0074] In addition, a QR code is attached to the groove of the taking plate 325 to record the usage time so as to facilitate timely reminder for replacement.

[0075] Reference Figure 1 and Figure 2, the mixing cups 33 are arranged in a straight line, and the mixing cups 33, the position calibration point 12, the first hole 24, the second hole 231, the third hole 232 and the fourth hole 233 are all located on the same straight line. Among them, the position calibration point 12 is located on the side of the cleaning tank 21 away from the mixing tank 31.

[0076] Therefore, after the automated machine aligns the sampling needle with the position calibration point 12, the position coordinates of each mixing cup 33 can be automatically adjusted, thus simplifying the calibration process.

[0077] Reference Figure 1 and Figure 2 The reagent cleaning and mixing device further includes a spray assembly 4, which includes a spray pool 41. The spray pool 41 is opened on the upper side of the device body 1, and the spray pool 41 is located on the side of the cleaning pool 21 away from the mixing pool 31 along the length direction of the device body 1. Therefore, the cleaning pool 21, the mixing pool 31 and the spray pool 41 are arranged in a straight line along the length direction of the device body 1. A negative pressure suction hole 42 is opened at the bottom of the spray pool 41, and the negative pressure suction hole 42 is opened in the vertical direction, and the lower side of the negative pressure suction hole 42 is connected to the waste liquid hole 25.

[0078] In the process of preparing the biological sample, in addition to cleaning the sampling needle, the glass slide also needs to be cleaned. In order to ensure the flushing effect of the carrier slide, it is often flushed through a spray head. Before the normal use of the spray head, there is some air in the spray head. Directly using the spray head to discharge the spray liquid will make the spray liquid spray unsmooth or uneven. Therefore, when using the spray head, pre-spray it in the spray pool first, so that the air in the spray head can be discharged, thereby ensuring the normal spraying of the spray head. The negative pressure suction hole 42 can accelerate the discharge of the spray waste liquid in the spray pool 41.

[0079] In the present application, the cleaning pool 21 and the spray pool 31 are arranged adjacent to each other to ensure that the non-toxic waste liquid in the spray pool 21 and the non-toxic waste liquid in the spray pool 31 both flow out from the waste liquid hole 25. The position of the mixing pool 31 is selected according to the actual situation, and the toxic waste liquid generated in the mixing pool 31 flows out from the drainage slot 314, ensuring that the drainage slot 314 is not connected to the waste liquid hole 25.

[0080] Preferably, refer to Figure 1 and Figure 2 , the cleaning pool 21, the mixing pool 31 and the spraying pool 41 are arranged in a straight line, the mixing pool 31 and the spraying pool 41 are located on opposite sides of the cleaning pool 21, or the mixing pool 31 and the cleaning pool 21 are located on opposite sides of the spraying pool 41. When the cleaning pool 21, the mixing pool 31 and the spraying pool 41 are arranged in a straight line, when the automated machine performs reagent mixing, the automated machine can move in a straight line between cleaning, mixing and spraying, and can quickly select the corresponding step to complete the corresponding process.

[0081] In addition, under the premise that the negative pressure suction hole 42 in the spray pool 41 is connected to the waste liquid hole 25, the cleaning pool 21, the mixing pool 31 and the spray pool 41 are staggered with each other, which can also meet the above requirements.

[0082] Reference Figure 1 , a metal shielding plate 11 is also provided on the outside of the device body 1. Therefore, in the process of cleaning and mixing, in order to ensure the normal function of the reagent liquid level detection, the metal shielding plate 11 wrapped around the entire device body 1 is grounded. Since the liquid level detection adopts the method of detecting the capacitance value (the dielectric constants of air and liquid are different) to realize the liquid level detection function; the liquid level detection function is required in the mixing cup to detect the liquid level height of the reagent; the shielding sheet metal and the reagent needle form a stable capacitor structure, thereby improving the accuracy of liquid level detection.

[0083] The implementation principle of the embodiment of the present application is: first, by installing the cup holder 32 and the mixing cup 33 on the cup holder 32, the corresponding reagent or sample is sucked by the sampling needle, and then added to the mixing cup 33, and then the sampling needle is cleaned.

[0084] The cleaning process is as follows: the first step is to move the sampling needle into hole No. 1 24, and repeatedly clean the inner wall of the sampling needle in hole No. 1 24 by pumping out cleaning liquid through the syringe pump. The cleaning liquid is discharged to hole No. 3 232 through the sampling needle in a high-pressure state, so that the tip of the sampling needle can be cleaned in hole No. 3 232. The third step is to pump out the cleaning liquid after cleaning hole No. 3 232, and the cleaning liquid is discharged to hole No. 4 233 through the sampling needle in a high-pressure state, so that the outer wall of the sampling needle can be cleaned in hole No. 4 233. At the same time, during this process, excess waste liquid flows into hole No. 1 24 through the corresponding drainage groove 234, and is finally discharged.

[0085] The reagent mixing process is as follows: after adding the sample through the sampling needle, a turbid liquid composed of multiple reagents is formed in the mixing cup 33, the sampling needle is lowered to the bottom of the mixing cup 33, and after extracting part of the turbid liquid, the needle is lifted to the top of the liquid surface and then discharged, and finally the sampling needle is used to reciprocate and suck out the specified volume of the mixed liquid to achieve mixing. When there is too much liquid in the mixing cup 33, the excess liquid will overflow from the mixing cup 33 and then flow out through the overflow pipe 323.

[0086] Before the shower head rinses the glass slide, the shower head is turned on toward the shower pool 41 until the shower head sprays the spray liquid stably.

[0087] The present application also discloses an immunohistochemical instrument, comprising the above-mentioned reagent cleaning and mixing device.

[0088] The implementation principle of the embodiment of the present application is: through the above-mentioned reagent cleaning and mixing device, the sampling needle cleaning, sample mixing and spraying processes in the immunohistochemical instrument can be connected with each other, thereby improving the working efficiency of the immunohistochemical instrument.

[0089] The embodiments of this specific implementation are all preferred embodiments of the present application, and are not intended to limit the protection scope of the present application. The same components are represented by the same figure marks. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the protection scope of the present application.

Claims

1. A reagent cleaning and mixing device, characterized in that, It comprises a device body (1), a cleaning component (2) and a mixing component (3), wherein the cleaning component (2) and the mixing component (3) are both arranged on the device body (1); The cleaning assembly (2) comprises a plurality of cleaning holes (23) for cleaning the sampling needles, and the cleaning holes (23) are all opened on the upper side of the device body (1); The mixing assembly (3) comprises a cup holder (32), a plurality of mixing cups (33) are arranged on the cup holder (32), and the cup holder (32) is arranged on the device body (1).

2. A reagent cleaning and mixing device according to claim 1, characterized in that: It also comprises a spray assembly (4), wherein the cleaning assembly (2), the mixing assembly (3) and the spray assembly (4) are all arranged on the device body (1), and the cleaning assembly (2) and the spray assembly (4) are arranged adjacent to each other.

3. A reagent cleaning and mixing device according to claim 2, characterized in that: The cleaning assembly (2) comprises a cleaning pool (21), the pool bottom of the cleaning pool (21) is provided with a No. 1 hole (24) for cleaning the inner wall of the sampling needle, the side wall of the No. 1 hole (24) is provided with a waste liquid hole (25) for collecting waste liquid, and the waste liquid hole (25) passes through the device body (1).

4. A reagent cleaning and mixing device according to claim 3, characterized in that: The spray assembly (4) comprises a spray pool (41), a negative pressure suction hole (42) is provided at the bottom of the spray pool (41), and the negative pressure suction hole (42) is connected to the waste liquid hole (25).

5. A reagent cleaning and mixing device according to claim 1, characterized in that: The cleaning component (2), the mixing component (3) and the spraying component (4) are arranged in a straight line.

6. A reagent cleaning and mixing device according to claim 1, characterized in that: A cleaning pool (21) is provided on the upper side of the device body (1), a cleaning platform (22) is provided on the inner wall of the cleaning pool (21), and a plurality of cleaning holes (23) are provided on the upper side of the cleaning platform (22) and are arranged in a straight line.

7. A reagent cleaning and mixing device according to claim 6, characterized in that: A position calibration point (12) is provided on the device body (1), and the position calibration point (12) is located on the straight line where the cleaning hole (23) is located.

8. A reagent cleaning and mixing device according to claim 7, characterized in that: The plurality of mixing cups (33) are all located on the same straight line, and the straight line on which the plurality of mixing cups (33) are located coincides with the straight line on which the plurality of cleaning holes (23) are located.

9. A reagent cleaning and mixing device according to any one of claims 1 to 8, characterized in that: A metal shielding plate (11) is sleeved on the outer side of the device body (1).

10. An immunohistochemical instrument, characterized in that: include: A reagent cleaning and mixing device as described in any one of claims 1 to 9 above.

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