Nucleic acid amplification detection device

Abstract

The utility model belongs to the technical field of nucleic acid amplification detection, and particularly relates to a nucleic acid amplification detection device, which comprises a shell, a reaction bin, a detection device and a control device, wherein the upper end surface of the shell is provided with a test tube insertion reaction bin; the at least one detection mechanism comprises a multi-channel transmitting assembly and a multi-channel receiving assembly which are arranged on the outer side of the test tube detection position in the reaction bin; wherein the multi-channel emission assembly emits at least one beam of excitation light into the test tube to enable a reagent in the test tube to generate fluorescence, and the multi-channel receiving assembly receives the corresponding fluorescence and detects the intensity corresponding to each fluorescence; by arranging the detection mechanism, the functional requirement of double-channel fluorescence multi-joint detection is met on the basis of single-channel fluorescence detection, the application range is wide, the detection device can be used for multi-joint detection, and the requirements can be met by one-time detection experiment aiming at different targets.

Description

Technical Field

[0001] The utility model belongs to the technical field of reagent detection, and specifically relates to a nucleic acid amplification detection device. Background Art

[0002] Nucleic acid amplification detection is a method of amplifying the nucleic acid sequence to be tested through the action of enzymes and then detecting it. The principle of multi-enzyme isothermal rapid amplification technology (MIRA) is to mainly use proteins such as recombinase, polymerase, and single-stranded binding protein to specifically amplify and generate new chains using primers and templates under a constant temperature of 37-42°C: the recombinase primer binds to form a recombinase-primer complex. After the complex is located in the homologous region of the primer, the recombinase unwinds the double-stranded structure and promotes the chain exchange between the primer and the template to form a D-loop structure; the single-stranded binding protein stabilizes the D-loop structure; the DNA polymerase binds to the primer to initiate DNA amplification to form an amplicon.

[0003] The utility model with patent number CN202322059377.1 discloses a constant temperature nucleic acid amplification analyzer, which belongs to the field of medical testing equipment, including a shell, a main control board and a detection unit are installed inside the shell, and operation buttons are arranged on the surface of the shell, and the detection unit and the display screen are both connected to the main control board signal; the detection unit includes a reaction chamber located inside the shell, and the reaction chamber includes a chamber body and a heating module located on one side of the chamber body, a light excitation element is arranged at the bottom of the chamber body, and a light receiving element is arranged on the side, and an excitation light of a fixed wavelength is generated by the light excitation element to excite the matching MIRA constant temperature reagent to generate fluorescence, and then the fluorescence intensity is detected by the light receiving element, and the positive and negative result judgment is calculated by the difference method, and its optical path structure is a single channel with a fixed single wavelength, which cannot be suitable for the needs of multi-wavelength multi-joint detection.

[0004] Therefore, a nucleic acid amplification detection device is designed to solve the technical problem that the constant temperature nucleic acid amplification analyzer in the prior art cannot be used for multi-wavelength multi-joint detection. Utility Model Content

[0005] The purpose of the utility model is to provide a nucleic acid amplification detection device to solve the above technical problems.

[0006] In order to solve the above technical problems, the utility model provides a nucleic acid amplification detection device, comprising:

[0007] The housing has an upper end surface provided with a reaction chamber for inserting a test tube; and

[0008] At least one detection mechanism, which includes a multi-channel transmitting component and a multi-channel receiving component arranged outside the test tube detection position in the reaction chamber; wherein

[0009] The multi-channel emitting component emits at least one beam of excitation light into the test tube to make the reagent in the test tube generate fluorescence, and the multi-channel receiving component receives the corresponding fluorescence and detects the intensity corresponding to each fluorescence.

[0010] Further, the multi-channel transmitting component includes:

[0011] An emission dichroic mirror is tilted and disposed below the cuvette detection position; and

[0012] At least two emission units to form multi-channel excitation light; wherein

[0013] At least one beam of excitation light passes through the emission dichroic mirror to be emitted from the bottom of the cuvette detection position.

[0014] Furthermore, each of the transmitting units comprises:

[0015] A light emitting element, the light emitting end of which is provided with an emission filter;

[0016] When the multi-channel emission assembly includes two emission units, each light emitting element is arranged at approximately 90°; wherein

[0017] The excitation light emitted by at least one of the light emitting elements is filtered by a corresponding emission filter and then refracted by an emission dichroic mirror to be emitted from the bottom of the test tube detection position.

[0018] Furthermore, a first convex lens is provided between the test tube detection position and the emission dichroic mirror;

[0019] The first convex lens is suitable for converging the excitation light passing through the emission dichroic mirror to the bottom of the test tube detection position.

[0020] Further, the multi-channel receiving component includes:

[0021] A receiving dichroic mirror is tilted and arranged on one side of the test tube detection position; and

[0022] At least two receiving units form a multi-channel, which are used to receive the fluorescence generated by the reagent in the tube;

[0023] At least one receiving unit receives the fluorescence that has passed through the receiving dichroic mirror to detect the intensity of the fluorescence.

[0024] Furthermore, each of the receiving units comprises:

[0025] A receiving element, wherein a receiving filter is provided at the light receiving end thereof;

[0026] When the multi-channel receiving assembly includes two receiving units, each receiving member is arranged at approximately 90°;

[0027] At least one of the receiving elements receives the fluorescence filtered by the corresponding receiving filter to detect the intensity of the fluorescence.

[0028] Furthermore, a second convex lens is provided between the test tube detection position and the receiving dichroic mirror;

[0029] The second convex lens is suitable for converging the fluorescence generated by the reagent in the tube onto the receiving dichroic mirror.

[0030] Furthermore, a mixing mechanism is provided inside the housing, which includes:

[0031] The motor is arranged inside the housing and at one side of the test tube detection position;

[0032] a turntable connected to the output end of the motor and having a magnetic block disposed thereon; and

[0033] A mixing element is pre-set in the test tube; wherein

[0034] The magnetic block is suitable for rotating when the motor is started, and drives the mixing element to perform reciprocating motion in the test tube through magnetic attraction.

[0035] The beneficial effect of the utility model is that the utility model realizes the functional requirements of dual-channel fluorescence multi-detection on the basis of single-channel fluorescence detection by providing a detection mechanism, has a wide range of applications, can be used for multi-detection, and can meet the requirements for different targets in one detection experiment.

[0036] Other features and advantages of the utility model will be described in the following description, and partly become apparent from the description, or understood by practicing the utility model. The purpose and other advantages of the utility model are realized and obtained by the structures particularly pointed out in the description and the drawings.

[0037] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, preferred embodiments are given below and described in detail with reference to the accompanying drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the specific implementation methods of the utility model or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the utility model. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0039] Figure 1 It is a schematic diagram of the overall three-dimensional structure of the utility model;

[0040] Figure 2This is a three-dimensional diagram of the cross-sectional structure of the multi-channel detection mechanism of the utility model;

[0041] Figure 3 It is a sectional plan view of the multi-channel detection mechanism of the utility model.

[0042] In the figure:

[0043] 1. Shell;

[0044] 2. Test tube; 20. Reaction chamber;

[0045] 3. Testing agency;

[0046] 31. Multi-channel emission assembly; 310. Emission fixing frame; 311. Light emitting element; 312. Emission filter; 313. Emission dichroic mirror; 314. First convex lens;

[0047] 32. Multi-channel receiving assembly; 320. Receiving fixing frame; 321. Receiving member; 322. Receiving filter; 323. Receiving dichroic mirror; 324. Second convex lens;

[0048] 4. Mixing mechanism; 40. Motor; 41. Turntable; 42. Magnetic block; 43. Mixing element. DETAILED DESCRIPTION

[0049] In order to make the purpose, technical solution and advantages of the embodiments of the utility model clearer, the technical solution of the utility model will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments of the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0050] The existing nucleic acid amplification detection device has a light excitation element at the bottom of its chamber and a light receiving element on the side. The light excitation element generates excitation light of a fixed wavelength to excite the matching MIRA constant temperature reagent to produce fluorescence, and then the fluorescence intensity is detected by the light receiving element. The positive and negative results are calculated by the difference method. Its optical path structure is a single channel with a fixed single wavelength, which cannot meet the needs of multi-wavelength multi-joint detection.

[0051] In order to solve the above technical problems, in at least one embodiment, a nucleic acid amplification detection device is provided, comprising: a housing 1, the upper end surface of which is provided with a reaction chamber 20 for inserting a test tube 2; and at least one detection mechanism 3, which comprises a multi-channel transmitting component 31 and a multi-channel receiving component 32 arranged outside the test tube detection position in the reaction chamber 20; wherein the multi-channel transmitting component 31 transmits at least one beam of excitation light into the test tube 2 to make the reagent in the test tube 2 generate fluorescence, and the multi-channel receiving component 32 receives the corresponding fluorescence and detects the intensity corresponding to each fluorescence;

[0052] By setting up a detection mechanism, the functional requirements of dual-channel fluorescence multiplex detection can be realized on the basis of single-channel fluorescence detection. It has a wide range of applications and can be used for multiplex detection. The requirements can be met in one detection experiment for different targets.

[0053] In some embodiments, the operator uses a pipette or a quantitative dropper to add 50ul of purified sample liquid into the test tube 2 in advance. For the outer shell 1, a flip cover can be set on the upper end surface of the outer shell 1, above the reaction chamber 20, and the test tube 2 is placed in the reaction chamber 20, and the flip cover is closed for sealing. For the reaction chamber 20, its internal cavity fits the contour of the outer wall of the test tube, which is the test tube detection position.

[0054] In some embodiments, an emission fixture 310 is disposed directly below the test tube detection position. When the test tube 2 is located at the test tube detection position, the light emitting element 311 (the light emitting element may preferably be an LED light emitting element) is activated to emit an excitation light, such as Figures 2 to 3 As shown, preferably, two light-emitting components 311 can be provided, both of which are fixed on the emission fixture 310, emitting different excitation light. Both light-emitting ends are provided with emission filters 312 to filter the emitted excitation light. One of the light-emitting components 311 is located in the same horizontal plane as the emission dichroic mirror 313 obliquely set in the emission fixture 310, and the other light-emitting component 311 is located directly below the emission dichroic mirror 313. The excitation light emitted by the two light-emitting components 311 is refracted by the emission dichroic mirror 313 and irradiated on the first convex lens 314. The excitation light is converged by the first convex lens 314 and emitted from the bottom of the test tube detection position to irradiate the reagent in the test tube 2 and excite the reagent to produce fluorescence.

[0055] In some embodiments, a receiving fixture 320 is fixed inside the housing 1 at one side of the test tube detection position. When the reagent in the test tube 2 generates fluorescence, the fluorescence will pass through the second convex lens 324 at one side of the test tube detection position and be collected by the second convex lens 324 on the receiving dichroic mirror 323. The receiving dichroic mirror 323 is tilted and fixed inside the receiving fixture 320 to refract the fluorescence toward the two receiving parts 321, such as Figures 2 to 3As shown, the number of receiving elements 321 is preferably two (the receiving element is preferably a photodetector), one of the receiving elements 321 is located directly below the receiving dichroic mirror 323, and the other receiving element 321 and the receiving dichroic mirror 323 are located in the same horizontal plane, and a receiving filter 322 is fixed to the light receiving ends of both, and the receiving filter 322 filters the refracted fluorescence and is received by the corresponding receiving element 321, and each receiving element 321 detects its corresponding fluorescence intensity, and calculates the positive and negative detection result according to the principle of the endpoint method.

[0056] In some embodiments, the angle between the two light-emitting elements 311 is approximately 90°, and different excitation lights are emitted. To avoid mutual interference between the excitation lights, the angle between the two light-emitting elements 311 is preferably 90°; the angle between the two receiving elements 321 is approximately 90°, and the angle between the two receiving elements 321 is preferably 90°.

[0057] In some embodiments, in order to accelerate the mixing of the reagents in the test tube 2, a mixing piece 43 (preferably a steel ball) is pre-packaged in the test tube 2. When the test tube 2 is placed in the test tube detection position, the motor 40 is started (the motor is preferably a DC motor), and its output end drives the turntable 41 and the magnetic block 42 fixed on the turntable 41 to rotate. The magnetic block 42 adsorbs the mixing piece 43, driving the mixing piece 43 to reciprocate in the vertical direction in the test tube 2, replacing the manual mixing method of shaking the test tube 2 up and down in the prior art to perform the reagent mixing operation, so as to accelerate the mixing of the reagents in the test tube 2.

[0058] To sum up, compared with the single-channel fluorescence detection adopted in the prior art, the functional requirements of multi-channel fluorescence multi-detection are realized on the basis of single-channel fluorescence detection. It has a wide range of applications and can be used for multi-detection. It can meet the requirements of one detection experiment for different targets, and adds a mixing function. In the process of sample reagent amplification, a step of automatic mixing operation is added, so that the DNA amplicons in the sample can be better mixed and amplified, the sensitivity of reagent detection is increased, and the manual mixing operation of personnel and the normative requirements of manual mixing operation are saved.

[0059] In the description of the embodiments of the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0060] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0061] In addition, each functional unit in each embodiment of the present invention may be integrated into one processing unit, or each unit may exist physically separately, or two or more units may be integrated into one unit.

[0062] Based on the above ideal embodiments of the utility model, the relevant staff can make various changes and modifications without departing from the technical concept of the utility model through the above description. The technical scope of the utility model is not limited to the content of the specification, and its technical scope must be determined according to the scope of the claims.

Claims

1. A nucleic acid amplification detection device, characterized in that: include: The housing has an upper end surface provided with a reaction chamber for inserting a test tube; and At least one detection mechanism, which includes a multi-channel transmitting component and a multi-channel receiving component arranged outside the test tube detection position in the reaction chamber; wherein The multi-channel emitting component emits at least one beam of excitation light into the test tube to make the reagent in the test tube generate fluorescence, and the multi-channel receiving component receives the corresponding fluorescence and detects the intensity corresponding to each fluorescence.

2. The nucleic acid amplification detection device according to claim 1, characterized in that: The multi-channel transmitting assembly comprises: An emission dichroic mirror is tilted and disposed below the cuvette detection position; and At least two emission units to form multi-channel excitation light; wherein At least one beam of excitation light passes through the emission dichroic mirror to be emitted from the bottom of the cuvette detection position.

3. The nucleic acid amplification detection device according to claim 2, characterized in that: Each of the transmitting units comprises: A light emitting element, the light emitting end of which is provided with an emission filter; When the multi-channel emission assembly includes two emission units, each light emitting element is arranged at approximately 90°; wherein The excitation light emitted by at least one of the light emitting elements is filtered by a corresponding emission filter and then refracted by an emission dichroic mirror to be emitted from the bottom of the test tube detection position.

4. The nucleic acid amplification detection device according to claim 3, characterized in that: A first convex lens is arranged between the test tube detection position and the emission dichroic mirror; The first convex lens is suitable for converging the excitation light passing through the emission dichroic mirror to the bottom of the test tube detection position.

5. The nucleic acid amplification detection device according to claim 1, characterized in that: The multi-channel receiving component comprises: A receiving dichroic mirror is tilted and arranged on one side of the test tube detection position; and At least two receiving units form a multi-channel, which are used to receive the fluorescence generated by the reagent in the tube; At least one receiving unit receives the fluorescence that has passed through the receiving dichroic mirror to detect the intensity of the fluorescence.

6. The nucleic acid amplification detection device according to claim 5, characterized in that: Each of the receiving units comprises: A receiving element, wherein a receiving filter is provided at the light receiving end thereof; When the multi-channel receiving assembly includes two receiving units, each receiving member is arranged at approximately 90°; At least one of the receiving elements receives the fluorescence filtered by the corresponding receiving filter to detect the intensity of the fluorescence.

7. The nucleic acid amplification detection device according to claim 6, characterized in that: A second convex lens is arranged between the test tube detection position and the receiving dichroic mirror; The second convex lens is suitable for converging the fluorescence generated by the reagent in the tube onto the receiving dichroic mirror.

8. The nucleic acid amplification detection device according to claim 1, characterized in that: A mixing mechanism is provided inside the housing, which comprises: The motor is arranged inside the housing and at one side of the test tube detection position; a turntable connected to the output end of the motor and having a magnetic block disposed thereon; and A mixing element is pre-set in the test tube; wherein The magnetic block is suitable for rotating when the motor is started, and drives the mixing element to perform reciprocating motion in the test tube through magnetic attraction.

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