Efficient solid-phase polypeptide synthesis device

Abstract

The utility model relates to the technical field of polypeptide synthesis, and discloses an efficient solid-phase polypeptide synthesis device which comprises a synthesis barrel, a barrel cover is arranged at the top of the synthesis barrel, barrel legs are arranged at the bottom of the synthesis barrel, a transverse plate is fixedly connected to the left side of the synthesis barrel, and stabilizing legs are fixedly connected to the bottom of the transverse plate. An adjusting column is fixedly connected to the top of the transverse plate, a first motor is fixedly connected to the top of the adjusting column, a lifting groove is formed in the right side of the adjusting column, a lead screw is arranged in the lifting groove, and a lifting block is in threaded connection with the surface of the lead screw. According to the efficient solid-phase polypeptide synthesis device, when the lead screw rotates, the lifting block can move up and down along the lead screw, the right side of the lifting block is fixedly connected with the barrel cover, and when the first motor drives the lead screw to rotate, the barrel cover can move up and down, so that the barrel cover can be conveniently opened and closed; and cleaning of the interior of the synthesis barrel is facilitated, manual opening of the barrel cover is avoided, and the manual labor amount is reduced.

Description

Technical Field

[0001] The utility model relates to the technical field of polypeptide synthesis, in particular to a high-efficiency solid-phase polypeptide synthesis device. Background Art

[0002] Peptides are compounds formed by α-amino acids linked together by peptide bonds. They are intermediate products of protein hydrolysis. Compounds formed by dehydration condensation of two amino acid molecules are called dipeptides. By analogy, there are tripeptides, tetrapeptides, pentapeptides, etc. Peptides composed of three or more amino acid molecules are called polypeptides.

[0003] A solid phase peptide synthesis device is provided in the Chinese utility model patent application disclosure specification CN 210974485 U, comprising a peptide synthesis cylinder with an opening on the upper side and a cylinder cover with a sealing cover arranged on the upper end of the peptide synthesis cylinder, a lower connecting rod is fixedly connected to the lower end of the cylinder cover at an eccentric position, two parallel interception nets are fixedly connected to the outer wall of the lower connecting rod, and a surrounding plate is fixedly connected to the edge of the interception net. By arranging two interception nets arranged up and down, during nitrogen stirring, large bubbles formed by nitrogen will be torn into small bubbles by the interception net located at the upper end, reducing the degree of surging, greatly reducing the phenomenon of splashing and wall adhesion due to nitrogen, and improving the purity of the product; after the excess air in the peptide synthesis cylinder is pressed out, the nitrogen circulation stirs the reactant and the resin, and the volatilized reaction liquid will also circulate, effectively avoiding the possibility of the reactant being lost to the outside due to its volatility, and further improving the purity of the product.

[0004] However, the above-mentioned prior art requires manual operation or the use of other instruments to open or close the sealing cover, which is inconvenient. Therefore, an efficient solid-phase peptide synthesis device is urgently needed. Utility Model Content

[0005] The purpose of the utility model is to provide a high-efficiency solid-phase peptide synthesis device to solve the problems raised in the above-mentioned background technology.

[0006] In order to solve the above technical problems, the utility model provides the following technical solutions: an efficient solid phase peptide synthesis device, comprising a synthesis barrel, a barrel cover is arranged on the top of the synthesis barrel, and barrel legs are arranged on the bottom of the synthesis barrel;

[0007] A horizontal plate is fixedly connected to the left side of the synthetic barrel, a stabilizing leg is fixedly connected to the bottom of the horizontal plate, an adjusting column is fixedly connected to the top of the horizontal plate, a first motor is fixedly connected to the top of the adjusting column, a lifting slot is opened on the right side of the adjusting column, a screw rod is arranged in the lifting slot, and a lifting block is threadedly connected to the surface of the screw rod.

[0008] Preferably, the lifting block is slidably arranged in the lifting slot, and the right side of the lifting block is fixedly connected to the barrel cover.

[0009] There is a horizontal plate on the left side of the synthetic barrel, and there are stabilizing legs at the bottom of the horizontal plate to increase stability. There is an adjusting column on the top of the horizontal plate, and a first motor is installed on the top of the adjusting column. There is a lifting slot on the right side of the adjusting column, and there is a screw rod in the lifting slot. There is a lifting block on the surface of the screw rod, and this lifting block is connected to the screw rod through a thread, so when the screw rod rotates, the lifting block will move up and down along the screw rod. The right side of the lifting block is fixedly connected to the barrel cover, so when the first motor drives the screw rod to rotate, the barrel cover will move up and down, so that the barrel cover can be easily opened and closed.

[0010] Preferably, the top of the screw rod movably passes through the adjustment column and is fixedly connected to the first motor, and the bottom of the screw rod is movably connected to the inner bottom wall of the lifting slot.

[0011] Preferably, a sealing gasket is provided inside the barrel cover, and a feed hopper is provided on the top of the barrel cover.

[0012] There is a sealing gasket inside the barrel cover to increase the sealing performance of the barrel cover, and there is a feeding hopper on the top of the barrel cover to add raw materials into the synthesis barrel.

[0013] Preferably, a mounting ring is fixedly connected to the interior of the synthesis barrel, a mesh plate is threadedly connected to the interior of the mounting ring, a discharge pipe is provided at the bottom of the synthesis barrel, and a discharge valve is provided on the discharge pipe.

[0014] Preferably, an air inlet pipe is provided on the right side of the synthesis barrel, an air inlet valve is provided on the air inlet pipe, an aeration head is provided on the left side of the air inlet pipe which passes through the synthesis barrel, and an air outlet pipe is provided on the right side of the synthesis barrel, an air outlet valve is provided on the air outlet pipe.

[0015] There is a discharge pipe at the bottom of the synthesis barrel, and there is a discharge valve on the discharge pipe for controlling the discharge. There is an air inlet pipe on the right side of the synthesis barrel, and there is an air inlet valve on the air inlet pipe. The left side of the air inlet pipe extends into the synthesis barrel, and there is an aeration head, which can be used to introduce nitrogen gas into the synthesis barrel. At the same time, there is also an air outlet pipe on the right side of the synthesis barrel, and there is an air outlet valve on the air outlet pipe for controlling the discharge of gas.

[0016] Preferably, the top of the barrel cover is fixedly connected to a second motor, the output end of the second motor is fixedly connected to a rotating rod, the bottom of the rotating rod movably passes through the barrel cover and is provided with a blade fan, and both ends of the rotating rod are fixedly connected to connecting rods, and both ends of the connecting rod are respectively fixedly connected to scrapers.

[0017] Compared with the prior art, the beneficial effects achieved by the utility model are:

[0018] First, the utility model has a horizontal plate on the left side of the synthetic barrel, and the bottom of the horizontal plate has stabilizing legs to increase stability, and the top of the horizontal plate has an adjusting column, and the top of the adjusting column is equipped with a first motor, and the right side of the adjusting column has a lifting groove, and the lifting groove has a screw rod, and the surface of the screw rod has a lifting block, and the lifting block is connected to the screw rod through a thread, so when the screw rod rotates, the lifting block will move up and down along the screw rod, and the right side of the lifting block is fixedly connected to the barrel cover, so when the first motor drives the screw rod to rotate, the barrel cover will move up and down, so that the barrel cover can be easily opened and closed, which facilitates the opening and closing of the barrel cover, facilitates the cleaning of the inside of the synthetic barrel, and avoids manually opening the barrel cover, thereby reducing the amount of manual labor.

[0019] Second, the utility model has a sealing gasket inside the barrel cover for increasing the sealing performance of the barrel cover, a feed hopper on the top of the barrel cover for adding raw materials into the synthesis barrel, a mounting ring inside the synthesis barrel, a mesh plate inside the mounting ring, the mesh plate can be conveniently installed and disassembled through threads, a discharge pipe at the bottom of the synthesis barrel, a discharge valve on the discharge pipe for controlling the discharge, an air inlet pipe on the right side of the synthesis barrel, an air inlet valve on the air inlet pipe, an aeration head on the left side of the air inlet pipe extending into the synthesis barrel, and a gas outlet head for introducing nitrogen gas into the synthesis barrel, and at the same time, an air outlet pipe on the right side of the synthesis barrel, an air outlet valve on the air outlet pipe for controlling the discharge of gas, a second motor on the top of the barrel cover, a rotating rod at the output end of the second motor, the bottom of the rotating rod extending into the synthesis barrel, and a blade fan for stirring the material in the synthesis barrel to accelerate the reaction of polypeptide synthesis. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the three-dimensional structure of the utility model;

[0021] Figure 2 This is a schematic diagram of the front cutaway structure of the adjustment column of the utility model;

[0022] Figure 3 This is a schematic diagram of the front cutaway structure of the barrel cover of the utility model;

[0023] Figure 4 This is a front cutaway structural diagram of the composite barrel and barrel cover of the utility model.

[0024] Among them: 1. synthetic barrel; 2. barrel cover; 3. barrel legs; 11. horizontal plate; 12. stabilizing legs; 13. adjusting column; 14. first motor; 15. screw rod; 16. lifting block; 21. sealing gasket; 22. feed hopper; 17. mounting ring; 18. mesh plate; 19. discharge pipe; 4. air inlet pipe; 5. aeration head; 6. air outlet pipe; 23. second motor; 24. rotating rod; 25. blade fan; 26. scraper. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the utility model to clearly and completely describe the technical solutions in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the utility model.

[0026] The utility model provides the following technical solutions:

[0027] Embodiment 1

[0028] See also Figure 1 , Figure 2 , a high-efficiency solid-phase peptide synthesis device, comprising a synthesis barrel 1, a barrel cover 2 is arranged on the top of the synthesis barrel 1, and a barrel leg 3 is arranged on the bottom of the synthesis barrel 1;

[0029] A horizontal plate 11 is fixedly connected to the left side of the synthetic barrel 1, a stabilizing leg 12 is fixedly connected to the bottom of the horizontal plate 11, an adjusting column 13 is fixedly connected to the top of the horizontal plate 11, a first motor 14 is fixedly connected to the top of the adjusting column 13, a lifting groove is opened on the right side of the adjusting column 13, a screw rod 15 is arranged in the lifting groove, and a lifting block 16 is threadedly connected to the surface of the screw rod 15.

[0030] The lifting block 16 is slidably disposed in the lifting slot, and the right side of the lifting block 16 is fixedly connected to the barrel cover 2 .

[0031] The top of the screw rod 15 movably passes through the adjustment column 13 and is fixedly connected to the first motor 14 , and the bottom of the screw rod 15 is movably connected to the inner bottom wall of the lifting slot.

[0032] Through the above technical scheme, there is a horizontal plate 11 on the left side of the synthesis barrel 1, and the bottom of the horizontal plate 11 has a stabilizing leg 12 to increase stability. There is an adjusting column 13 on the top of the horizontal plate 11, and a first motor 14 is installed on the top of the adjusting column 13. There is a lifting groove on the right side of the adjusting column 13, and a screw rod 15 is arranged in the lifting groove. There is a lifting block 16 on the surface of the screw rod 15, and this lifting block 16 is connected to the screw rod 15 through a thread. Therefore, when the screw rod 15 rotates, the lifting block 16 will move up and down along the screw rod 15. The right side of the lifting block 16 is fixedly connected to the barrel cover 2, so when the first motor 14 drives the screw rod 15 to rotate, the barrel cover 2 will move up and down, so that the barrel cover 2 can be easily opened and closed, which facilitates the opening and closing of the barrel cover 2, facilitates the cleaning of the inside of the synthesis barrel 1, and avoids manually opening the barrel cover 2, thereby reducing the amount of manual labor.

[0033] Embodiment 2

[0034] See also Figure 3 , Figure 4, and on the basis of the first embodiment, it is further obtained that: a sealing gasket 21 is arranged inside the barrel cover 2, and a feed hopper 22 is arranged on the top of the barrel cover 2.

[0035] A mounting ring 17 is fixedly connected to the interior of the synthesis barrel 1, and a mesh plate 18 is threadedly connected to the interior of the mounting ring 17. A discharge pipe 19 is provided at the bottom of the synthesis barrel 1, and a discharge valve is provided on the discharge pipe 19.

[0036] An air inlet pipe 4 is arranged on the right side of the synthesis barrel 1, and an air inlet valve is arranged on the air inlet pipe 4. The left side of the air inlet pipe 4 runs through the synthesis barrel 1 and is provided with an aeration head 5. An air outlet pipe 6 is arranged on the right side of the synthesis barrel 1, and an air outlet valve is arranged on the air outlet pipe 6.

[0037] A second motor 23 is fixedly connected to the top of the barrel cover 2, and a rotating rod 24 is fixedly connected to the output end of the second motor 23. The bottom of the rotating rod 24 movably passes through the barrel cover 2 and is provided with a blade fan 25. Both ends of the rotating rod 24 are fixedly connected to connecting rods, and both ends of the connecting rod are respectively fixedly connected to scrapers 26.

[0038] Through the above technical scheme, the inside of the barrel cover 2 is provided with a sealing gasket 21 for increasing the sealing performance of the barrel cover 2, the top of the barrel cover 2 is provided with a feed hopper 22 for adding raw materials into the synthesis barrel 1, the inside of the synthesis barrel 1 is provided with a mounting ring 17, the inside of the mounting ring 17 is provided with a mesh plate 18, the mesh plate 18 can be conveniently installed and disassembled through threads, the bottom of the synthesis barrel 1 is provided with a discharge pipe 19, the discharge pipe 19 is provided with a discharge valve for controlling the discharge, the right side of the synthesis barrel 1 is provided with an air inlet pipe 4, the air inlet pipe 4 is provided with an air inlet valve, the left side of the air inlet pipe 4 extends into the synthesis barrel 1, and an aeration head 5 is provided for introducing nitrogen gas into the synthesis barrel 1, and at the same time, the right side of the synthesis barrel 1 is provided with an air outlet pipe 6, the air outlet pipe 6 is provided with an air outlet valve for controlling the discharge of gas, the top of the barrel cover 2 is provided with a second motor 23, the output end of the second motor 23 is provided with a rotating rod 24, the bottom of the rotating rod 24 extends into the synthesis barrel 1, and a blade fan 25 is provided for stirring the material in the synthesis barrel 1 to accelerate the reaction of polypeptide synthesis.

[0039] In actual operation, when the device is in use, when the first motor 14 drives the screw rod 15 to rotate, the lifting block 16 moves in the lifting groove to move the barrel cover 2, so that the barrel cover 2 is covered on the synthesis barrel 1. The sealing gasket 21 is provided to increase the sealing of the barrel cover 2, and the raw materials are fed from the feed hopper 22. There is a second motor 23 on the top of the barrel cover 2, and the output end of the second motor 23 has a rotating rod 24. The bottom of the rotating rod 24 extends into the synthesis barrel 1, and there is a blade fan 25 for stirring the material in the synthesis barrel 1 to accelerate the reaction of polypeptide synthesis. There is an air inlet pipe 4 on the right side of the barrel, and there is an air inlet valve on the air inlet pipe 4. The left side of the air inlet pipe 4 extends into the synthesis barrel 1, and there is an aeration head 5, which can be used to introduce nitrogen gas into the synthesis barrel 1. The nitrogen gas passes through the mesh plate 18 to accelerate the efficiency of solid-phase polypeptide synthesis, and the mesh plate 18 filters the material so that the waste liquid passes through the mesh plate 18 and is discharged from the discharge pipe 19.

[0040] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit thereof, and the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. An efficient solid phase peptide synthesis device, comprising a synthesis barrel (1), characterized in that: The top of the synthetic barrel (1) is provided with a barrel cover (2), and the bottom of the synthetic barrel (1) is provided with barrel legs (3); The left side of the synthetic barrel (1) is fixedly connected to a transverse plate (11), the bottom of the transverse plate (11) is fixedly connected to a stabilizing leg (12), the top of the transverse plate (11) is fixedly connected to an adjusting column (13), the top of the adjusting column (13) is fixedly connected to a first motor (14), a lifting groove is provided on the right side of the adjusting column (13), a screw rod (15) is provided in the lifting groove, and a lifting block (16) is threadedly connected to the surface of the screw rod (15); The interior of the synthesis barrel (1) is fixedly connected with a mounting ring (17), the interior of the mounting ring (17) is threadedly connected with a mesh plate (18), a discharge pipe (19) is provided at the bottom of the synthesis barrel (1), and a discharge valve is provided on the discharge pipe (19); An air inlet pipe (4) is arranged on the right side of the synthesis barrel (1), and an air inlet valve is arranged on the air inlet pipe (4); the left side of the air inlet pipe (4) passes through the synthesis barrel (1) and is provided with an aeration head (5); an air outlet pipe (6) is arranged on the right side of the synthesis barrel (1), and an air outlet valve is arranged on the air outlet pipe (6); The top of the barrel cover (2) is fixedly connected to a second motor (23), the output end of the second motor (23) is fixedly connected to a rotating rod (24), the bottom of the rotating rod (24) movably passes through the barrel cover (2) and is provided with a blade fan (25), and both ends of the rotating rod (24) are fixedly connected to connecting rods, and both ends of the connecting rod are respectively fixedly connected to scrapers (26).

2. A high-efficiency solid-phase peptide synthesis device according to claim 1, characterized in that: The lifting block (16) is slidably arranged in the lifting slot, and the right side of the lifting block (16) is fixedly connected to the barrel cover (2).

3. A high-efficiency solid-phase peptide synthesis device according to claim 1, characterized in that: The top of the screw rod (15) movably penetrates the adjustment column (13) and is fixedly connected to the first motor (14), and the bottom of the screw rod (15) is movably connected to the inner bottom wall of the lifting slot.

4. The high-efficiency solid-phase peptide synthesis device according to claim 1, characterized in that: A sealing gasket (21) is arranged inside the barrel cover (2), and a feeding hopper (22) is arranged on the top of the barrel cover (2).

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