Plant pathogen spore capturing and analyzing device

Abstract

The utility model discloses a plant germ spore capturing and analyzing device, and belongs to the technical field of agricultural monitoring. Comprising a photographing bin and a culture bin which are arranged in a box body, and a glass slide belt driven by a power piece, openings are formed in the side faces of the photographing bin and the culture bin, and the glass slide belt sequentially passes through the culture bin and the photographing bin through the openings. A constant-temperature assembly is arranged in the culture bin; the upper portion of the culture bin communicates with an air inlet assembly. According to the utility model, the problems of inconvenience in operation, influence on precision and the like caused by single collection mode of conventional spore capturing equipment are solved; the glass slide and the glass slide belt are combined, advantages and disadvantages are complementary, and the effects of fast and convenient data acquisition and high data acquisition precision are achieved.

Description

Technical Field

[0001] The utility model relates to the technical field of agricultural monitoring, in particular to a device for capturing and analyzing plant pathogenic bacteria spores. Background Art

[0002] There are two main types of spore collection devices on the market: glass slides and glass tapes. Glass slide collection cannot achieve large-scale collection due to equipment limitations, and the glass slides need to be frequently replenished. Glass tape collection can solve this problem.

[0003] For example, referring to the patent application number CN202320879013.5, our company has previously designed a glass carrier tape assembly for a spore capture instrument; it includes an active chuck and a passive chuck rotatably arranged on one side of a mounting plate, and a glass carrier tape roll is mounted on the active chuck and the passive chuck; a first groove wheel and a second groove wheel are horizontally arranged on the mounting plate; and a camera is installed on the mounting plate through a displacement assembly. The device uses a glass carrier tape to capture and analyze spores, and has the characteristics of sustainable capture, low cost, easy maintenance, and convenient replacement.

[0004] However, after a long period of testing and use, it was found that this previous device still had some problems.

[0005] 1. The glass carrier tape is exposed as a whole. The uncollected glass carrier tape is easily contaminated by the air outlet of the fan, resulting in low collection accuracy and may affect the test results.

[0006] 2. The collected data cannot be reviewed again; and, because the collected spores may be interfered with by the camera or dust, the data collection images are unclear and cannot be manually checked, resulting in inaccurate data.

[0007] 3. The glass slide assembly of the whole spore capture instrument does not have a constant temperature culture area, and the collected spore pests and diseases are relatively small, which may not be captured by a microscope.

[0008] 4. Due to the use of two x and y motion devices, the assembly requirements of the equipment are high and the assembly cost is high.

[0009] 5. When photographing spores, the electron microscope needs to focus precisely, and without a separate photo chamber, it is easy to be disturbed.

[0010] Simply put, when the spore capture and analysis device uses glass slides for collection, the slides need to be frequently replenished; when the spores are collected using glass tapes, if the photos are not clear, the spores on the glass tapes cannot be manually reviewed more accurately.

[0011] Therefore, our company improved the previous equipment and designed a new device for capturing and analyzing plant pathogen spores. Utility Model Content

[0012] The utility model aims to solve the above problems in the prior art and proposes a device for capturing and analyzing plant pathogenic bacteria spores.

[0013] In order to achieve the above purpose, the utility model adopts the following technical solutions:

[0014] The plant pathogen spore capture and analysis device comprises a photographing chamber, a culture chamber, and a glass carrier belt driven by a power member arranged in a box;

[0015] The sides of the photographing chamber and the culture chamber are provided with openings, and the slide tape passes through the culture chamber and the photographing chamber in sequence through the openings;

[0016] A constant temperature component is provided in the culture chamber;

[0017] The top of the culture bin is connected to the air inlet assembly.

[0018] In some embodiments, a vertically movable camera is disposed above the photo chamber;

[0019] A fill light is arranged below the photographing chamber.

[0020] In some embodiments, the constant temperature component includes a temperature controller, and a heating element and a cooling element disposed in the culture chamber.

[0021] In some embodiments, it further includes: a slide collection chamber;

[0022] The air outlet of the air inlet assembly is connected to the top of the slide collection chamber.

[0023] In some embodiments, the interior of the slide collection chamber is provided with a track extending through and extending forward and backward;

[0024] A tray is slidably arranged on the track, and a glass slide is placed on the tray.

[0025] In some embodiments, the power member includes an active chuck and a passive chuck;

[0026] The glass carrier tape is clamped on the passive chuck in the form of a glass carrier tape roll, and the glass carrier tape passes through the culture chamber and the photographing chamber in sequence and then is wound on the active chuck.

[0027] In some embodiments, the air inlet assembly includes a fan, an air inlet pipe, and an air outlet pipe.

[0028] In some embodiments, the air inlet duct is connected to the hood.

[0029] In some embodiments, a door is provided on the front side of the box body;

[0030] A panel is provided at the rear of the box door;

[0031] The panel is provided with a glass slide replacement door and a glass slide taking and placing opening.

[0032] In some embodiments, a mounting plate is provided in the box;

[0033] The photographing chamber and the culture chamber are detachably arranged on the mounting plate.

[0034] Compared with the prior art, the utility model provides a plant pathogen spore capture and analysis device, which has the following beneficial effects.

[0035] 1. The utility model solves the problems of the conventional spore capturing equipment having a single collection method, causing inconvenience in operation and affecting accuracy. The slide and the slide tape are combined to complement each other's advantages and disadvantages, so as to achieve the effect of quick and convenient data collection and high data accuracy.

[0036] 2. The utility model adds a culture chamber, a photographing chamber, and a slide collection chamber to solve the problem that the uncollected slides are easily contaminated by the air outlet of the fan, resulting in low collection accuracy; it solves the problem that the spores have not been cultured and are too small to be observed by the camera and cannot be observed clearly, etc.

[0037] 3. The utility model can provide a constant temperature culture chamber with cooling in summer and heating in winter, thereby obtaining a good spore culture environment. Moreover, the whole structure is simpler, easy to assemble, and more stable in structure; each component operates stably, data collection is reliable, and the use and maintenance costs are reduced.

[0038] Other advantages, objectives and features of the present invention will be described in the following description to some extent; and will be apparent to those skilled in the art based on the following examination and study to some extent; or, may be taught from the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0039] Figure 1 A schematic diagram of the overall structure.

[0040] Figure 2 This is a schematic diagram of the state where the box door is removed.

[0041] Figure 3 This is a schematic diagram of the panel removal status.

[0042] Figure 4 This is a schematic diagram of the internal structure.

[0043] Figure 5 Schematic diagram of the internal rear structure.

[0044] Figure 6This is a schematic diagram of the state where the air inlet component is removed.

[0045] Figure 7 This is a schematic diagram of the structure of the slide collection chamber.

[0046] Figure 8 An exploded view of the slide collection chamber.

[0047] Fig. 9 It is a schematic diagram of the structure of the tray and a schematic diagram of the rear structure.

[0048] Fig.10 Schematic diagram of the state of the collection chamber after the slide is removed.

[0049] Fig.11 This is a schematic diagram of the state where the outer shells of the photography chamber and the culture chamber are removed.

[0050] Fig.12 This is a schematic diagram of the structure of the photo booth.

[0051] Fig.13 This is a schematic diagram of the structure of the culture chamber.

[0052] Fig.14 It is a structural diagram of the mounting plate.

[0053] Fig.15 This is a schematic diagram of the lower side of the culture chamber shell.

[0054] Fig.16 This is a schematic diagram of the rear side of the culture chamber shell.

[0055] In the figure:

[0056] 1. Box body;

[0057] 11. Box door; 12. Panel; 13. Glass slide replacement door; 14. Glass slide take-out and put-out port;

[0058] 2. Photo booth;

[0059] 21. Camera; 22. Fill light;

[0060] 3. Cultivation chamber;

[0061] 31. Air inlet; 32. Air outlet;

[0062] 4. Glass tape;

[0063] 5. Constant temperature components;

[0064] 51. Temperature controller; 52. Heating element; 53. Refrigeration element;

[0065] 6. Air inlet assembly;

[0066] 61. Fan; 62. Air inlet pipe; 63. Air outlet pipe; 64. Air branch pipe; 65. Air cap;

[0067] 7. Slide collection chamber;

[0068] 71. track; 72. tray; 73. slide; 74. slide press; 75. handle;

[0069] 8. Power parts;

[0070] 81. Active chuck; 82. Passive chuck; 83. Driving motor; 84. Rotary damper;

[0071] 9. Install the plate. DETAILED DESCRIPTION

[0072] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0073] Reference Figure 1-16 The plant pathogen spore capture and analysis device includes a photographing chamber 2, a culture chamber 3, and a glass carrier belt 4 driven by a power member 8, which are arranged in a box body 1.

[0074] Among them, openings are set on the sides of the photographing chamber 2 and the culture chamber 3, and the glass carrier belt 4 passes through the culture chamber 3 and the photographing chamber 2 in turn through the openings; spores are collected in the culture chamber 3 and cultured to make them grow; then, photos are taken in the closed environment in the photographing chamber 2, and the obtained photos are stored locally or transmitted to the cloud through the communication component.

[0075] It can be understood that in the closed environment of the photographing chamber 2 and the culture chamber 3, the glass slide 4 can be prevented from being contaminated, thereby ensuring the accuracy and reliability of the data.

[0076] Rollers for supporting and positioning the glass slide 4 are arranged on both sides of the culture chamber 3 and the photographing chamber 2 .

[0077] The roller is a grooved wheel, the groove size of which is slightly larger than the width of the glass carrier 4, and at least one is provided at each of the two sides; preferably, one is provided at each of the two sides and between the culture chamber 3 and the photographing chamber 2; Fig.11 As shown, three sheaves are provided.

[0078] A constant temperature component 5 is arranged in the culture chamber 3 to provide a constant temperature environment; it can be refrigerated and kept at a constant temperature in summer and heated and kept at a constant temperature in winter, thereby forming a good spore culture environment.

[0079] An air inlet 31 is provided above the culture chamber 3 and connected to the air inlet assembly 6 to inhale air containing spores. At the same time, an air outlet 32 ​​is provided at the rear of the culture chamber 3; by providing the air inlet 31 and the air outlet 32, rapid air circulation is achieved to improve collection efficiency.

[0080] In some embodiments, a vertically movable camera 21 is disposed above the photographing chamber 2 for taking pictures.

[0081] It is understandable that the camera 21 cooperates with the microscope to realize the photographing of spores; the setting here is a conventional form in the field and will not be repeated.

[0082] Furthermore, a fill light 22 is provided below the photographing chamber 2 .

[0083] By arranging a large area of ​​LED fill light inside the photographing chamber 2, the light source is supplemented in microscope photography to obtain clearer and more reliable photos.

[0084] Furthermore, the camera 21 is moved by a Z-axis driving member.

[0085] The Z-axis driving component includes: a lead screw arranged at the output end of the stepping motor, a moving seat threadably connected to the lead screw, and a fixed bracket adapted for the camera 21 .

[0086] The camera 21 is detachably mounted on the mobile base via a fixed bracket.

[0087] In some embodiments, the constant temperature component 5 includes a temperature controller 51 , and a heating element 52 and a cooling element 53 disposed in the culture chamber 3 .

[0088] Preferably, the heating element 52 is a ceramic heating plate, and the cooling element 53 is a semiconductor cooling plate, which are controlled by the temperature controller 51 .

[0089] It is understandable that a temperature sensor is also provided in the culture chamber 3; according to the data collected by the temperature sensor, the chamber is cooled or heated to maintain the set temperature.

[0090] In some embodiments, it also includes: a glass slide collection chamber 7; the air outlet of the air inlet component 6 is connected to the top of the glass slide collection chamber 7 to send the spores into the glass slide collection chamber 7.

[0091] Specifically, a track 71 extending frontward and rearward is provided inside the glass slide collection chamber 7 ; a tray 72 is slidably provided on the track 71 , and a glass slide 73 is provided on the tray 72 .

[0092] The slide glass 73 is carried by the tray 72 and is taken out or placed.

[0093] Furthermore, a pressing sheet 74 is provided at the front end of the tray 72 to press and fix the glass slide 73 .

[0094] Preferably, a handle 75 is provided in front of the tray 72 to facilitate operation.

[0095] In some embodiments, the power member 8 includes an active chuck 81 and a passive chuck 82 .

[0096] The glass carrier tape 4 is mounted on the passive chuck 82 in the form of a glass carrier tape roll. The glass carrier tape 4 passes through the culture chamber 3 and the photographing chamber 2 in sequence and then is wound on the roll on the active chuck 81 .

[0097] It can be understood that the active chuck 81 is driven by a driving motor 83 , and the passive chuck 82 is provided with a rotation damper 84 ; when working, the passive chuck 82 is driven to rotate by the glass carrier belt 4 .

[0098] In some embodiments, the air inlet assembly 6 includes a fan 61 , an air inlet pipe 62 , and an air outlet pipe 63 .

[0099] Furthermore, two air branch pipes 64 are provided on the air outlet pipe 63 ; after the spores are collected by the fan 61 , the two air branch pipes 64 divide the air flow into two, one end of which is blown onto the glass slide 4 and the other end is blown onto the glass slide 73 .

[0100] In some embodiments, the air inlet pipe 62 is connected to the air hood 65 .

[0101] The hood 65 is located at the top of the whole and is provided with air holes.

[0102] In some embodiments, a box door 11 is provided on the front side of the box body 1; the box door 11 may also be provided on the other side surfaces, or a detachable baffle may be provided.

[0103] In some embodiments, a panel 12 is disposed behind the door 11 .

[0104] The panel 12 is vertically arranged and fixed in the box; a display screen and an operation keyboard are arranged on the panel 12 according to actual needs.

[0105] In some embodiments, the panel 12 is provided with a glass slide replacement door 13 and a glass slide access opening 14 .

[0106] The glass carrier tape replacement door 13 corresponds to the positions of the active chuck 81 and the passive chuck 82 ; after the glass carrier tape replacement door 13 is opened, the glass carrier tape roll can be replaced quickly and conveniently.

[0107] The opening shape of the slide glass taking-in and putting-out opening 14 can be matched with the cross section of the tray 72 ; the tray 72 can be put in or taken out through the opening of the slide glass taking-in and putting-out opening 14 .

[0108] In some embodiments, a mounting plate 9 is provided in the box body 1; the photographing chamber 2 and the culture chamber 3 are detachably provided on the mounting plate 9. Fig.14 As shown, the mounting plate 9 is provided with mounting positions, openings and other structures of various components, providing a mounting basis.

[0109] In some embodiments, Fig.15 , 16 As shown; the culture chamber 3 is divided into a collection part and a culture part; that is, the culture chamber 3 is divided into two parts by a partition, the air inlet 31 and the air outlet 32 ​​are arranged in the collection part, and the constant temperature component 5 is located in the culture part.

[0110] In this form, spores are collected in the collection part, and air flows in and out at the same time; then, the glass carrier belt 4 carries the spores to the culture part for constant temperature culture; by separating the collection and culture, contamination on the glass carrier belt 4 can be further avoided; and the culture part is more closed, which is more conducive to the culture of spores.

[0111] When the utility model is used, spores on the glass belt 4 and the glass slide 73 can be collected at the same time; a high-precision microscope is used above the glass belt 4 in combination with an electronic eyepiece to take photos of the spores, and the real-time collection of plant spores is achieved through uploading on the network, so that the collected data can be obtained conveniently and quickly; the spores collected on the glass slide 73 are manually removed and the glass slide 73 is sent to the research laboratory to more accurately judge the situation of the plant pathogen spores. Through the setting of the device, the data collection of the spore capture device can be efficient and fast, while ensuring the accuracy of the data.

[0112] The utility model solves the problems that the conventional spore capturing equipment has a single collection method, which causes inconvenience in operation and affects the accuracy. The slide 73 and the slide belt 4 are combined to complement each other's advantages and disadvantages, so as to achieve the effect of quick and convenient data collection and high data accuracy.

[0113] In the utility model, a culture chamber 3, a photographing chamber 2, and a slide collection chamber 7 are added to the existing structure of the previous patent "spore capture instrument slide assembly", so as to solve the problem that the uncollected slide 4 is easily contaminated by the fan outlet, resulting in low collection accuracy; and solve the problem that the spores are not cultured and the volume is too small, so the camera cannot observe them and the observation is not clear. At the same time, the constant temperature culture chamber 3 can be refrigerated and kept at a constant temperature in summer, and heated and kept at a constant temperature in winter, so as to obtain a good spore culture environment. Moreover, the whole is simpler, easy to assemble, and the structure is more stable; each component operates stably, and data collection is reliable; the use and maintenance costs are reduced.

[0114] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes within the technical scope disclosed by the present invention according to the technical scheme and the utility model concept of the present invention, which should be covered by the protection scope of the present invention.

[0115] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0116] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the utility model. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0117] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and cannot be understood as limitations of the present invention. Ordinary technicians in the field can change, modify, replace and modify the above embodiments within the scope of the present invention.

Claims

1. A device for capturing and analyzing plant pathogenic bacteria spores, characterized in that: It comprises a photographing chamber (2), a culture chamber (3), and a glass carrier belt (4) driven by a power member (8) which are arranged in a box body (1); The sides of the photographing chamber (2) and the culture chamber (3) are provided with openings, and the glass slide (4) passes through the openings and passes through the culture chamber (3) and the photographing chamber (2) in sequence; A constant temperature component (5) is provided in the culture chamber (3); The top of the culture bin (3) is connected to an air inlet assembly (6).

2. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: A vertically movable camera (21) is arranged above the photographing chamber (2); A fill light (22) is provided below the photographing chamber (2).

3. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: The constant temperature component (5) comprises a temperature controller (51), and a heating element (52) and a cooling element (53) arranged in the culture chamber (3).

4. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: Also includes: Slide collection chamber (7); The air outlet of the air inlet assembly (6) is connected to the top of the glass slide collection chamber (7).

5. The plant pathogen spore capture and analysis device according to claim 4, characterized in that: The interior of the glass slide collection chamber (7) is provided with a track (71) extending through the front and rear; A tray (72) is slidably disposed on the track (71), and a glass slide (73) is placed on the tray (72).

6. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: The power member (8) comprises an active chuck (81) and a passive chuck (82); The glass carrier tape (4) is clamped on the passive chuck (82) in the form of a glass carrier tape roll. The glass carrier tape (4) passes through the culture chamber (3) and the photographing chamber (2) in sequence and then is wound on the active chuck (81).

7. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: The air inlet assembly (6) comprises a fan (61), an air inlet pipe (62), and an air outlet pipe (63).

8. The plant pathogen spore capture and analysis device according to claim 7, characterized in that: The air inlet pipe (62) is connected to the inside of the air hood (65).

9. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: A box door (11) is provided on the front side of the box body (1); A panel (12) is provided at the rear of the box door (11); The panel (12) is provided with a glass slide replacement door (13) and a glass slide taking and placing opening (14).

10. The plant pathogen spore capture and analysis device according to claim 1, characterized in that: A mounting plate (9) is provided in the box body (1); The photographing chamber (2) and the culture chamber (3) are detachably arranged on the mounting plate (9).

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