Three-dimensional slice scanning immersion inspection structure

Abstract

The utility model discloses a three-dimensional slice scanning immersion detection structure which comprises an immersion detection bin used for bearing an immersion detection medium for detection. The displacement table is arranged in the immersion inspection bin in a lifting manner, and the displacement table is used for bearing a sample; the illumination objective lens is arranged in the immersion inspection bin, the optical axis of the illumination objective lens and the illumination path are collinear, and the illumination objective lens is used for emitting a light beam to illuminate a sample; the imaging objective lens is arranged in the immersion detection bin, the optical axis of the imaging objective lens is collinear with the detection path, and the imaging objective lens is used for collecting light emitted by the sample; the illumination objective lens and the imaging objective lens are immersed in an immersion detection medium, a certain angle is formed between the imaging objective lens and the illumination objective lens, and the displacement table passes through the intersection of the detection path and the illumination path when ascending and descending. The scanning imaging device has the advantage of being capable of scanning and imaging a sample with a large thickness.

Description

Technical Field

[0001] The utility model relates to the technical field of three-dimensional scanning detection, in particular to a three-dimensional slice scanning immersion detection structure. Background Art

[0002] In recent years, methods for processing large biological samples (such as whole mouse brain and spinal cord) and three-dimensional microscopic imaging methods have gradually emerged. Selective plane illumination microscopy (SPIM) is a new imaging device that breaks the diffraction limit and serves the above three-dimensional microscopic imaging methods. It can be used for rapid three-dimensional fluorescence imaging of transparent large tissues.

[0003] Selective plane illumination microscopy (SPIM) illuminates the sample by forming a light sheet, and can scan and detect the optical section of the sample in the vertical direction for imaging. In the existing SPIM, the sample is generally immersed in a sample chamber with an immersion medium for detection. However, in the prior art, the illumination objective lens and the imaging objective lens are generally arranged outside the chamber, so that the space for the sample to move in the vertical direction is very limited, resulting in the thickness of the sample that can be detected by the prior art being very limited, and it is difficult to exceed a thickness of 3 mm.

[0004] In view of this, the purpose of the utility model is to provide a new technical solution to solve the existing technical problems. Utility Model Content

[0005] In order to overcome the deficiencies of the prior art, the utility model provides a three-dimensional slice scanning immersion inspection structure, which solves the problem that the prior inspection structure can only inspect samples with a relatively thin thickness.

[0006] The technical solution adopted by the utility model to solve its technical problems is:

[0007] A three-dimensional slice scanning immersion inspection structure, comprising:

[0008] An immersion inspection chamber, the immersion inspection chamber is used to carry an immersion inspection medium for inspection;

[0009] A displacement stage, which can be raised and lowered in the immersion inspection chamber and is used to carry samples;

[0010] An illumination objective lens, wherein the illumination objective lens is disposed in the immersion inspection chamber, and an optical axis of the illumination objective lens is colinear with an illumination path, and the illumination objective lens is used to emit a light beam to illuminate the sample;

[0011] An imaging objective lens, which is disposed in the immersion inspection chamber, and whose optical axis is collinear with the detection path, and is used to collect light emitted by the sample;

[0012] The illumination objective lens and the imaging objective lens are immersed in the immersion test medium, and the imaging objective lens and the illumination objective lens are arranged at a certain angle. When the displacement stage is lifted or lowered, it passes through the intersection of the detection path and the illumination path.

[0013] In the above structure, the angle formed by the imaging objective lens and the illumination objective lens is 90°.

[0014] In the above structure, a sample box is detachably provided on the displacement stage, the sample is placed in the sample box, and at least the bottom of the sample box is a light-transmitting structure.

[0015] In the above structure, the sample box includes a box body and a box cover matched with the box body, a sample cavity is formed between the box cover and the box body, and the sample is placed in the sample cavity.

[0016] In the above structure, the sample box is fixed to the translation stage by magnetic attraction.

[0017] In the above structure, the two opposite sides of the immersion inspection chamber are inclined to form a first inclined plane and a second inclined plane, the first inclined plane is provided with a first mounting hole for mounting an illumination objective lens, and the second inclined plane is provided with a second mounting hole for mounting an imaging objective lens.

[0018] In the above structure, it also includes an objective lens mounting frame, the immersion inspection chamber is provided with a connecting boss, the objective lens mounting frame is fixedly mounted on the connecting boss, and the illumination objective lens and the imaging objective lens are arranged on the objective lens mounting frame.

[0019] In the above structure, a Z-axis translation mounting seat is provided on the objective lens mounting frame, and the imaging objective lens is mounted on the Z-axis translation mounting seat.

[0020] In the above structure, a deflection adjustment component is also included, the Z-axis translation mounting seat is rotatably connected to the objective lens mounting frame, and the deflection adjustment component includes an adjustment screw, the adjustment screw is threadedly connected to the objective lens mounting frame, and one end abuts against the Z-axis translation mounting seat.

[0021] In the above structure, the deflection adjustment assembly further comprises a return spring, one end of which is connected to the Z-axis translation mounting seat, and the other end of which is connected to the objective lens mounting frame.

[0022] The beneficial effect of the utility model is that the illumination objective lens and the imaging objective lens are arranged in the immersion inspection chamber and immersed in the immersion inspection medium, so that the immersion inspection chamber is freed from the limitation of the connection and installation of the illumination objective lens and the imaging objective lens, which is conducive to the setting of a larger immersion inspection chamber, so that the displacement stage can move a larger distance in the vertical direction, and thus can scan samples of larger thickness. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The utility model is further described below in conjunction with the accompanying drawings and embodiments.

[0024] Figure 1 It is a partial structural schematic diagram of the utility model;

[0025] Figure 2 It is a schematic diagram of the cross-sectional structure of the utility model;

[0026] Figure 3 It is a schematic diagram of the split structure of the utility model;

[0027] Figure 4 This is a schematic diagram of the structure of the mid-immersion inspection bin of the utility model;

[0028] Figure 5 This is a schematic diagram of the connection structure between the immersion inspection chamber and the objective lens mounting frame of the utility model;

[0029] Figure 6 This is a schematic structural diagram of the utility model from another angle;

[0030] Figure 7 yes Figure 6 Enlarged schematic diagram of part A in the middle.

[0031] Figure numerals: 1, immersion inspection chamber; 11, first inclined surface; 111, first mounting hole; 12, second inclined surface; 121, second mounting hole; 13, connecting boss; 2, illumination objective lens; 3, imaging objective lens; 4, translation stage; 41, sample box; 411, box cover; 412, box body; 5, objective lens mounting bracket; 51, Z-axis translation mounting seat; 52, deflection adjustment assembly; 521, adjustment screw; 522, return spring. DETAILED DESCRIPTION

[0032] The following is combined with Figure 1-7 The utility model is further described.

[0033] The following will clearly and completely describe the concept, specific structure and technical effects of the utility model in combination with the embodiments and drawings, so as to fully understand the purpose, characteristics and effects of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, not all of them. Based on the embodiments of the utility model, other embodiments obtained by technicians in this field without creative work are all within the scope of protection of the utility model. In addition, all the connection / connection relationships involved in the patent do not refer to the direct connection of components, but refer to the formation of a better connection structure by adding or reducing connection accessories according to the specific implementation situation. The various technical features in the creation of the utility model can be combined interchangeably without conflicting with each other.

[0034] Reference Figures 1 to 7The utility model provides a three-dimensional slice scanning immersion inspection structure, which improves the existing immersion inspection structure and breaks through the problem that the size of the immersion inspection chamber 1 is limited due to the existing lighting objective lens 2 and imaging objective lens 3 being arranged outside the immersion inspection chamber 1, resulting in the limited vertical movable distance of the sample to be tested and the thin sample detection thickness. The three-dimensional slice scanning immersion inspection structure includes an immersion inspection chamber 1, a displacement stage 4, an lighting objective lens 2 and an imaging objective lens 3, wherein the immersion inspection chamber 1 is used to carry the immersion inspection medium; the displacement stage 4 is used to carry the sample to be tested; the lighting objective lens 2 is used to emit a light beam to illuminate the sample to be tested; and the imaging objective lens 3 is used to collect the light emitted by the sample to be tested when it is illuminated.

[0035] Reference Figure 1 and Figure 2 Specifically, the illumination objective lens 2 is fixedly arranged in the immersion inspection chamber 1, and one end of the outgoing light beam of the illumination objective lens 2 extends into the immersion inspection chamber 1, that is, one end of the outgoing light beam of the illumination objective lens 2 is immersed in the immersion inspection medium, and the optical axis of the illumination objective lens 2 is collinear with the illumination path. The imaging objective lens 3 is fixedly arranged in the immersion inspection chamber 1, and one end of the imaging objective lens 3 receiving the light beam extends into the immersion inspection chamber 1, that is, one end of the illumination objective lens 2 receiving the light beam is immersed in the immersion inspection medium, and the optical axis of the imaging objective lens 3 is collinear with the imaging path. The illumination path and the imaging path intersect and form a certain angle, and the intersection of the two paths is the place where the image is clearest during the detection process, that is, the imaging focal plane. The displacement stage 4 can be raised and lowered and arranged on one side of the immersion inspection chamber 1, and the displacement stage 4 passes through the imaging focal plane of the detection path and the illumination path during the lifting process. At this time, the light sheet emitted from the illumination objective lens 2 irradiates and scans the sample, and the sample tissue structure is imaged, and as the displacement stage 4 rises and falls, a three-dimensional scanning image of the sample is formed. The structure driving the translation platform 4 to move up and down can be a screw lifting structure, a synchronous belt lifting structure, a guide rail slider structure, etc., which can realize the lifting function of the translation platform 4 as described above, and is not specifically limited here.

[0036] The illumination objective lens 2 and the imaging objective lens 3 are both arranged in the immersion inspection chamber 1. Compared with the case where the imaging objective lens 3 and the illumination objective lens 2 are both arranged outside the immersion inspection chamber 1, the size of the immersion inspection chamber 1 can be free from the restrictions of the positions of the illumination objective lens 2 and the imaging objective lens 3, which is conducive to designing the immersion inspection chamber 1 to be larger. The larger the size of the immersion inspection chamber 1 is, the greater the lifting distance of the displacement stage 4 in the vertical direction is, which is conducive to the detection and scanning of thicker samples.

[0037] In this embodiment, the angle formed by the intersection of the imaging path and the illumination path is 90°, that is, the illumination objective lens 2 and the imaging objective lens 3 are arranged perpendicularly.

[0038] Reference Figures 4 to 7Specifically, the side walls of the immersion inspection chamber 1 are inclined at opposite sides, which are the first inclined surface 11 and the second inclined surface 12. The first inclined surface 11 is provided with a first mounting hole 111, and the illumination objective lens 2 is fixedly mounted in the first mounting hole 111, and extends into the immersion inspection chamber 1 through the first mounting hole 111. The second inclined surface 12 is provided with a second mounting hole 121, and the imaging objective lens 3 is fixedly mounted in the second mounting hole 121, and extends into the immersion inspection chamber 1 through the second mounting hole 121. The aperture size of the first mounting hole 111 is consistent with the diameter size of the illumination objective lens 2, and the illumination objective lens 2 is fixed to the first mounting hole 111 by glue dispensing connection; the aperture size of the second mounting hole 121 is consistent with the diameter size of the imaging objective lens 3, and the imaging objective lens 3 is also fixed to the second mounting hole 121 by glue dispensing connection. The side walls of the immersion inspection chamber 1 are inclined at opposite sides to cooperate with the illumination objective lens 2 and the imaging objective lens 3, which is conducive to the installation and fixation of the illumination objective lens 2 and the imaging objective lens 3.

[0039] Furthermore, the three-dimensional slice scanning immersion inspection structure further includes an objective lens mounting frame 5, which is detachably fixedly connected to the immersion inspection chamber 1. Specifically, the immersion inspection chamber 1 is fixedly provided with connecting bosses 13 around it, and screw holes are provided on the connecting bosses 13. The objective lens mounting frame 5 is connected and fixed to the connecting bosses 13 by fasteners such as screws. The ends of the illumination objective lens 2 and the imaging objective lens 3 away from the immersion inspection chamber 1 are respectively fixedly connected to the objective lens mounting frame 5. In actual setting, the immersion inspection chamber 1 and the connecting bosses 13 can be set by integral molding to ensure the connection strength between the two.

[0040] Furthermore, a Z-axis translation mounting seat 51 is provided on the objective lens mounting frame 5, and the imaging objective lens 3 is mounted on the Z-axis translation mounting seat 51. The Z-axis translation mounting seat 51 is an optical mounting seat that can be precisely positioned along the optical axis. The imaging objective lens 3 is arranged on the Z-axis translation mounting seat 51 to achieve precise adjustment of the imaging objective lens 3 in the direction of its optical axis, so as to accurately determine the imaging focal plane. Since the Z-axis translation mounting seat 51 is a prior art, its structure and adjustment principle are not described in detail here.

[0041] Furthermore, the objective lens mounting frame 5 is also provided with a deflection adjustment assembly 52, which is used to fine-tune the deflection angle of the imaging objective lens 3. Specifically, the deflection adjustment assembly 52 includes an adjustment screw 521 and a return spring 522. The adjustment screw 521 is threadedly connected to the objective lens mounting frame 5, and one end of the adjustment screw 521 abuts against a side edge of the Z-axis translation mounting seat 51. One end of the return spring 522 is connected to the objective lens mounting frame 5, and the other end is connected to the Z-axis translation mounting seat 51, and the return spring 522 is arranged above the adjustment screw 521. Correspondingly, the Z-axis translation mounting seat 51 is rotatably connected to the objective lens mounting frame 5. When the adjusting screw 521 is rotated in the forward direction, the adjusting screw 521 moves toward the direction close to the Z-axis translation mounting seat 51 so that the adjusting screw 521 pushes the Z-axis translation mounting seat 51. The Z-axis translation mounting seat 51 is deflected under the pushing action of the adjusting screw 521. At this time, the elastic compression of the reset spring 522 is realized to adjust the deflection angle of the imaging objective lens 3; when the adjusting screw 521 is rotated in the reverse direction, the adjusting screw 521 moves toward the direction away from the Z-axis translation mounting seat 51. At this time, the elasticity of the reset spring 522 is gradually released, thereby driving the Z-axis translation mounting seat 51 to gradually reset.

[0042] When installing the lighting objective lens 2 and the imaging objective lens 3, first install the lighting objective lens 2 and the imaging objective lens 3 to the corresponding positions of the objective lens mounting frame 5, then connect and fix the objective lens mounting frame 5 to the immersion inspection chamber 1, and then adjust the position of the lighting objective lens 2. After the adjustment is completed, fix and seal the lighting objective lens 2 and the first mounting hole 111 with glue; then fine-tune the position of the imaging objective lens 3 through the Z-axis translation mounting seat 51 and the deflection adjustment assembly 52 to achieve the desired effect. After the adjustment is completed, fix and seal the lighting objective lens 2 and the second mounting hole 121 with glue.

[0043] Reference Figure 1 and Figure 3 Furthermore, a sample box 41 is provided on the displacement stage 4, and the sample box 41 is detachably fixed to the displacement stage 4. In this embodiment, the sample box 41 is fixed to the displacement stage 4 by magnetic attraction. The sample box 41 is detachably fixed to the displacement stage 4 by magnetic attraction, which is not only reliable in connection and alignment, but also convenient in installation and disassembly, which is conducive to taking and placing samples. The sample is placed in the sample box 41 for detection and scanning. At least the bottom of the sample box 41 is a light-transmitting structure. In actual setting, a light-transmitting material with a refractive index close to or the same as that of the immersion test medium can be selected to make the light-transmitting structure. Such a setting is conducive to reducing optical aberrations during scanning and imaging.

[0044] In this embodiment, the sample box 41 includes a box body 412 and a box cover 411. The inner side of the box body 412 cooperates with the box cover 411. A sample cavity is formed between the box body 412 and the box cover 411. The sample is placed in the sample cavity for scanning and imaging. At least the bottom of the box body 412 is a light-transmitting structure. The light sheet emitted by the illumination objective lens 2 can be irradiated from the light-transmitting structure onto the sample to illuminate the sample, and the light emitted by the sample can be imaged by the objective lens 3 structure from the light-transmitting structure. The box cover 411 and the box body 412 are detachably connected, and the connection method can be an interference fit, a hinged fit, or a snap-fit ​​connection, etc., and the connection structure between the box cover 411 and the box body 412 is not specifically limited here.

[0045] The above is a specific description of the preferred implementation of the utility model, but the invention of the utility model is not limited to the described embodiments. Technical personnel familiar with the field can also make various equivalent deformations or substitutions without violating the spirit of the utility model. These equivalent deformations or substitutions are all included in the scope defined by the claims of this application.

Claims

1. A three-dimensional slice scanning immersion inspection structure, characterized in that: include: An immersion inspection chamber, the immersion inspection chamber is used to carry an immersion inspection medium for inspection; A displacement stage, which can be raised and lowered in the immersion inspection chamber and is used to carry samples; An illumination objective lens, wherein the illumination objective lens is disposed in the immersion inspection chamber, and an optical axis of the illumination objective lens is colinear with an illumination path, and the illumination objective lens is used to emit a light beam to illuminate the sample; An imaging objective lens, which is disposed in the immersion inspection chamber, and whose optical axis is collinear with the detection path, and is used to collect light emitted by the sample; The illumination objective lens and the imaging objective lens are immersed in the immersion test medium, and the imaging objective lens and the illumination objective lens are arranged at a certain angle. When the displacement stage is lifted or lowered, it passes through the intersection of the detection path and the illumination path.

2. A three-dimensional slice scanning and immersion inspection structure according to claim 1, characterized in that: The angle formed by the imaging objective lens and the illumination objective lens is 90°.

3. A three-dimensional slice scanning and immersion inspection structure according to claim 1, characterized in that: A sample box is detachably arranged on the displacement table, a sample is placed in the sample box, and at least the bottom of the sample box is a light-transmitting structure.

4. A three-dimensional slice scanning and immersion inspection structure according to claim 3, characterized in that: The sample box comprises a box body and a box cover matched with the box body, a sample cavity is formed between the box cover and the box body, and the sample is placed in the sample cavity.

5. The three-dimensional slice scanning and immersion inspection structure according to claim 3, characterized in that: The sample box is fixed to the translation stage by magnetic attraction.

6. A three-dimensional slice scanning and immersion inspection structure according to claim 1, characterized in that: The two opposite sides of the immersion inspection chamber are inclined to form a first inclined plane and a second inclined plane. The first inclined plane is provided with a first mounting hole for mounting an illumination objective lens, and the second inclined plane is provided with a second mounting hole for mounting an imaging objective lens.

7. A three-dimensional slice scanning and immersion inspection structure according to claim 6, characterized in that: It also includes an objective lens mounting frame, a connecting boss is arranged on the immersion inspection chamber, the objective lens mounting frame is fixedly mounted on the connecting boss, and the illumination objective lens and the imaging objective lens are arranged on the objective lens mounting frame.

8. A three-dimensional slice scanning and immersion inspection structure according to claim 7, characterized in that: The objective lens mounting frame is provided with a Z-axis translation mounting seat, and the imaging objective lens is mounted on the Z-axis translation mounting seat.

9. A three-dimensional slice scanning and immersion inspection structure according to claim 8, characterized in that: It also includes a deflection adjustment component, the Z-axis translation mounting seat is rotatably connected to the objective lens mounting frame, and the deflection adjustment component includes an adjustment screw, the adjustment screw is threadedly connected to the objective lens mounting frame, and one end of the adjustment screw abuts against the Z-axis translation mounting seat.

10. The three-dimensional slice scanning and immersion inspection structure according to claim 9, characterized in that: The deflection adjustment assembly also includes a return spring, one end of which is connected to the Z-axis translation mounting seat, and the other end of which is connected to the objective lens mounting frame.

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